MS-Fit Search Results

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Sample ID (comment): HSG057
Database searched: SwissProt.r36
Molecular weight search (1000 - 100000 Da) selects 69977 entries.
Full pI range: 74019 entries.
Species search ( HUMAN MOUSE ) selects 8141 entries.
Combined molecular weight, pI and species searches select 7373 entries.
MS-Fit search selects 1670 entries (results displayed for top 5 matches).

Considered modifications: | Oxidation of M | Protein N-terminus Acetylated | Acrylamide Modified Cys |

Min. # Peptides
to Match
4

Peptide Mass
Tolerance (+/-)
50.000 ppm

Peptide Masses
are
monoisotopic

Digest
Used
Trypsin

Max. # Missed
Cleavages
4

Cysteines
Modified by
unmodified

Peptide
N terminus
Hydrogen (H)

Peptide
C terminus
Free Acid (O H)

Input #
Peptide Masses
67

Result Summary

Rank	MOWSE Score	# (%) Masses Matched	Protein MW (Da)/pI	Species	Protein Name
1	1.46e+007	15/67 (22%)	42644.5 / 5.34	HUMAN	CREATINE KINASE, B CHAIN (EC 2.7.3.2) (B-CK).
2	2.54e+005	13/67 (19%)	65886.8 / 8.16	HUMAN	KERATIN, TYPE II CYTOSKELETAL 1 (CYTOKERATIN 1) (K1) (CK 1) (67 KD CYTOKERATIN) (HAIR ALPHA PROTEIN).
3	4.76e+004	10/67 (14%)	42713.5 / 5.40	MOUSE	CREATINE KINASE, B CHAIN (EC 2.7.3.2) (B-CK).
4	2.7e+004	12/67 (17%)	61987.6 / 5.14	HUMAN	KERATIN, TYPE I CYTOSKELETAL 9 (CYTOKERATIN 9) (K9) (CK 9).
5	2.61e+003	5/67 (7%)	17738.9 / 8.49	HUMAN	CHORIOGONADOTROPIN BETA CHAIN PRECURSOR (CHORIONIC GONADOTROPHIN BETA SUBUNIT).

Detailed Results

1. 15/67 matches (22%). 42644.5 Da, pI = 5.34. Acc. # P12277. HUMAN. CREATINE KINASE, B CHAIN (EC 2.7.3.2) (B-CK)..

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
1031.5741	1031.5559	17.6400	359	366	(K)(L)
1232.6275	1232.6163	9.1245	87	96	(K)(H)
1258.6075	1258.5825	19.8420	139	148	(R)GFCLPPHCSR(G)	2Cys-am
1303.7389	1303.7262	9.7598	33	43	(K)(A)
1571.8462	1571.8143	20.2842	253	265	(R)FCTGLTQIETLFK(S)	1Cys-am
1586.8580	1586.8390	12.0064	157	172	(K)(Y)
1657.8383	1657.8338	2.6783	224	236	(K)(V)
1848.9499	1848.9781	-15.2803	342	358	(R)(L)
1928.9438	1928.9700	-13.5533	133	148	(R)TGRSIRGFCLPPHCSR(G)	2Cys-am
1964.9481	1964.9314	8.5358	321	341	(R)(L)
2169.9694	2169.9664	1.4120	14	32	(R)(V)
2184.0649	2184.1395	-34.1590	131	148	(R)VRTGRSIRGFCLPPHCSR(G)	2Cys-am
2439.1693	2439.1516	7.2689	12	32	(K)(V)
2455.1801	2455.1465	13.7015	12	32	(K)LRFPAEDEFPDLSAHNNHMAK(V)	1Met-ox
2455.1801	2455.2675	-35.6117	131	151	(R)VRTGRSIRGFCLPPHCSRGER(R)	1Cys-am
2518.2426	2518.1698	28.9106	108	130	(K)(V)

52 unmatched masses: 601.2207 644.2771 656.0851 745.4015 825.1079 842.5159 1065.5392 1109.5099 1140.5889 1157.6065 1165.6188 1179.6130 1234.6547 1277.7348 1300.5591 1307.7012 1383.7258 1475.7696 1493.7485 1515.7826 1517.7172 1638.8683 1655.8270 1698.7712 1707.7758 1716.8530 1790.8677 1791.7931 1834.8486 1837.9426 1851.9560 1890.9359 1940.9299 1954.0806 1993.9853 2173.0822 2211.0946 2234.0783 2283.2408 2284.1858 2299.0830 2383.9895 2510.1407 2705.1842 2717.1078 2872.3865 2873.4460 2895.3622 2903.4262 3223.2124 3224.4041 3265.5346

The matched peptides cover 45% (174/381 AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

2. 13/67 matches (19%). 65886.8 Da, pI = 8.16. Acc. # P04264. HUMAN. KERATIN, TYPE II CYTOSKELETAL 1 (CYTOKERATIN 1) (K1) (CK 1) (67 KD CYTOKERATIN) (HAIR ALPHA PROTEIN)..

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
1179.6130 1179.6010 10.1659 376 385 (K)(H)
1277.7348 1277.7105 18.9818 472 482 (K)(T)
1300.5591 1300.5302 22.1926 257 266 (K)(N)
1383.7258 1383.6909 25.2643 185 196 (K)(V)
1475.7696 1475.7858 -10.9765 199 210 (R)(W)
1475.7696 1475.7494 13.6788 211 222 (K)(T)
1638.8683 1638.8604 4.8229 185 198 (K)(F)
1657.8383 1657.7934 27.0531 12 28 (R)(R)
1716.8530 1716.8517 0.7905 417 431 (K)(G)
1954.0806 1954.0106 35.7894 386 402 (R)(V)
1993.9853 1993.9772 4.0789 223 238 (R)(R)
2173.0822 2173.1689 -39.8850 239 256 (R)(N)
2184.0649 2184.1260 -28.0061 441 459 (K)(L)
2383.9895 2383.9524 15.5495 518 548 (R)(G)

54 unmatched masses: 601.2207 644.2771 656.0851 745.4015 825.1079 842.5159 1031.5741 1065.5392 1109.5099 1140.5889 1157.6065 1165.6188 1232.6275 1234.6547 1258.6075 1303.7389 1307.7012 1493.7485 1515.7826 1517.7172 1571.8462 1586.8580 1655.8270 1698.7712 1707.7758 1790.8677 1791.7931 1834.8486 1837.9426 1848.9499 1851.9560 1890.9359 1928.9438 1940.9299 1964.9481 2169.9694 2211.0946 2234.0783 2283.2408 2284.1858 2299.0830 2439.1693 2455.1801 2510.1407 2518.2426 2705.1842 2717.1078 2872.3865 2873.4460 2895.3622 2903.4262 3223.2124 3224.4041 3265.5346

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)
1179.6130	1179.6010	10.1659	376	385	(K)(H)
1277.7348	1277.7105	18.9818	472	482	(K)(T)
1300.5591	1300.5302	22.1926	257	266	(K)(N)
1383.7258	1383.6909	25.2643	185	196	(K)(V)
1475.7696	1475.7858	-10.9765	199	210	(R)(W)
1475.7696	1475.7494	13.6788	211	222	(K)(T)
1638.8683	1638.8604	4.8229	185	198	(K)(F)
1657.8383	1657.7934	27.0531	12	28	(R)(R)
1716.8530	1716.8517	0.7905	417	431	(K)(G)
1954.0806	1954.0106	35.7894	386	402	(R)(V)
1993.9853	1993.9772	4.0789	223	238	(R)(R)
2173.0822	2173.1689	-39.8850	239	256	(R)(N)
2184.0649	2184.1260	-28.0061	441	459	(K)(L)
2383.9895	2383.9524	15.5495	518	548	(R)(G)

The matched peptides cover 31% (202/643 AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

3. 10/67 matches (14%). 42713.5 Da, pI = 5.40. Acc. # Q04447. MOUSE. CREATINE KINASE, B CHAIN (EC 2.7.3.2) (B-CK)..

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
1031.5741 1031.5559 17.6400 359 366 (K)(L)
1258.6075 1258.5825 19.8420 139 148 (R)GFCLPPHCSR(G) 2Cys-am
1303.7389 1303.7262 9.7598 33 43 (K)(A)
1571.8462 1571.8143 20.2842 253 265 (R)FCTGLTQIETLFK(S) 1Cys-am
1848.9499 1848.9781 -15.2803 342 358 (R)(L)
1928.9438 1928.9700 -13.5533 133 148 (R)TGRSIRGFCLPPHCSR(G) 2Cys-am
1964.9481 1964.9314 8.5358 321 341 (R)(L)
2184.0649 2184.1395 -34.1590 131 148 (R)VRTGRSIRGFCLPPHCSR(G) 2Cys-am
2455.1801 2455.1465 13.7015 12 32 (K)(V)
2455.1801 2455.2675 -35.6117 131 151 (R)VRTGRSIRGFCLPPHCSRGER(R) 1Cys-am
2518.2426 2518.1698 28.9106 108 130 (K)(V)

57 unmatched masses: 601.2207 644.2771 656.0851 745.4015 825.1079 842.5159 1065.5392 1109.5099 1140.5889 1157.6065 1165.6188 1179.6130 1232.6275 1234.6547 1277.7348 1300.5591 1307.7012 1383.7258 1475.7696 1493.7485 1515.7826 1517.7172 1586.8580 1638.8683 1655.8270 1657.8383 1698.7712 1707.7758 1716.8530 1790.8677 1791.7931 1834.8486 1837.9426 1851.9560 1890.9359 1940.9299 1954.0806 1993.9853 2169.9694 2173.0822 2211.0946 2234.0783 2283.2408 2284.1858 2299.0830 2383.9895 2439.1693 2510.1407 2705.1842 2717.1078 2872.3865 2873.4460 2895.3622 2903.4262 3223.2124 3224.4041 3265.5346

The matched peptides cover 35% (135/381 AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

4. 12/67 matches (17%). 61987.6 Da, pI = 5.14. Acc. # P35527. HUMAN. KERATIN, TYPE I CYTOSKELETAL 9 (CYTOKERATIN 9) (K9) (CK 9)..

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
745.4015 745.4208 -25.9302 368 373 (K)(H)
1065.5392 1065.4999 36.8559 154 162 (K)(L)
1157.6065 1157.5915 12.9940 250 260 (R)(Q)
1307.7012 1307.6782 17.5909 240 249 (R)(Q)
1791.7931 1791.7283 36.2074 490 512 (R)(G)
1837.9426 1837.9660 -12.7295 374 389 (R)(K)
1851.9560 1851.9275 15.3940 321 335 (K)(N)
2299.0830 2299.1202 -16.1759 271 289 (K)(N)
2510.1407 2510.1323 3.3456 449 471 (K)(I)
2705.1842 2705.1617 8.3488 63 94 (R)(G)
2895.3622 2895.3907 -9.8484 290 316 (K)(D)
3223.2124 3223.2821 -21.6244 579 618 (R)(S)

55 unmatched masses: 601.2207 644.2771 656.0851 825.1079 842.5159 1031.5741 1109.5099 1140.5889 1165.6188 1179.6130 1232.6275 1234.6547 1258.6075 1277.7348 1300.5591 1303.7389 1383.7258 1475.7696 1493.7485 1515.7826 1517.7172 1571.8462 1586.8580 1638.8683 1655.8270 1657.8383 1698.7712 1707.7758 1716.8530 1790.8677 1834.8486 1848.9499 1890.9359 1928.9438 1940.9299 1954.0806 1964.9481 1993.9853 2169.9694 2173.0822 2184.0649 2211.0946 2234.0783 2283.2408 2284.1858 2383.9895 2439.1693 2455.1801 2518.2426 2717.1078 2872.3865 2873.4460 2903.4262 3224.4041 3265.5346

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)
745.4015	745.4208	-25.9302	368	373	(K)(H)
1065.5392	1065.4999	36.8559	154	162	(K)(L)
1157.6065	1157.5915	12.9940	250	260	(R)(Q)
1307.7012	1307.6782	17.5909	240	249	(R)(Q)
1791.7931	1791.7283	36.2074	490	512	(R)(G)
1837.9426	1837.9660	-12.7295	374	389	(R)(K)
1851.9560	1851.9275	15.3940	321	335	(K)(N)
2299.0830	2299.1202	-16.1759	271	289	(K)(N)
2510.1407	2510.1323	3.3456	449	471	(K)(I)
2705.1842	2705.1617	8.3488	63	94	(R)(G)
2895.3622	2895.3907	-9.8484	290	316	(K)(D)
3223.2124	3223.2821	-21.6244	579	618	(R)(S)

The matched peptides cover 37% (231/622 AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

5. 5/67 matches (7%). 17738.9 Da, pI = 8.49. Acc. # P01233. HUMAN. CHORIOGONADOTROPIN BETA CHAIN PRECURSOR (CHORIONIC GONADOTROPHIN BETA SUBUNIT)..

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
1234.6547 1234.6683 -11.0115 154 165 (R)(-)
2439.1693 2439.2854 -47.5999 1 22 (-)(E)
2455.1801 2455.2803 -40.8102 1 22 (-)MEMFQGLLLLLLLSMGGTWASK(E) 1Met-ox
2717.1078 2717.2229 -42.3846 41 63 (K)EGCPVCITVNTTICAGYCPTMTR(V) 4Cys-am
2903.4262 2903.4629 -12.6417 89 115 (R)LPGCPRGVNPVVSYAVALSCQCALCRR(S) 1Cys-am

62 unmatched masses: 601.2207 644.2771 656.0851 745.4015 825.1079 842.5159 1031.5741 1065.5392 1109.5099 1140.5889 1157.6065 1165.6188 1179.6130 1232.6275 1258.6075 1277.7348 1300.5591 1303.7389 1307.7012 1383.7258 1475.7696 1493.7485 1515.7826 1517.7172 1571.8462 1586.8580 1638.8683 1655.8270 1657.8383 1698.7712 1707.7758 1716.8530 1790.8677 1791.7931 1834.8486 1837.9426 1848.9499 1851.9560 1890.9359 1928.9438 1940.9299 1954.0806 1964.9481 1993.9853 2169.9694 2173.0822 2184.0649 2211.0946 2234.0783 2283.2408 2284.1858 2299.0830 2383.9895 2510.1407 2518.2426 2705.1842 2872.3865 2873.4460 2895.3622 3223.2124 3224.4041 3265.5346

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
1234.6547	1234.6683	-11.0115	154	165	(R)(-)
2439.1693	2439.2854	-47.5999	1	22	(-)(E)
2455.1801	2455.2803	-40.8102	1	22	(-)MEMFQGLLLLLLLSMGGTWASK(E)	1Met-ox
2717.1078	2717.2229	-42.3846	41	63	(K)EGCPVCITVNTTICAGYCPTMTR(V)	4Cys-am
2903.4262	2903.4629	-12.6417	89	115	(R)LPGCPRGVNPVVSYAVALSCQCALCRR(S)	1Cys-am

The matched peptides cover 50% (83/165 AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):