MS-Fit Search Results

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Sample ID (comment): HSG058
Database searched: SwissProt.r36
Molecular weight search (1000 - 100000 Da) selects 69977 entries.
Full pI range: 74019 entries.
Species search ( HUMAN MOUSE ) selects 8141 entries.
Combined molecular weight, pI and species searches select 7373 entries.
MS-Fit search selects 860 entries (results displayed for top 5 matches).

Considered modifications: | Oxidation of M | Protein N-terminus Acetylated | Acrylamide Modified Cys |

Min. # Peptides
to Match
4

Peptide Mass
Tolerance (+/-)
50.000 ppm

Peptide Masses
are
monoisotopic

Digest
Used
Trypsin

Max. # Missed
Cleavages
4

Cysteines
Modified by
unmodified

Peptide
N terminus
Hydrogen (H)

Peptide
C terminus
Free Acid (O H)

Input #
Peptide Masses
49

Result Summary

Rank	MOWSE Score	# (%) Masses Matched	Protein MW (Da)/pI	Species	Protein Name
1	2.2e+004	13/49 (26%)	27745.3 / 4.73	HUMAN	14-3-3 PROTEIN ZETA/DELTA (PROTEIN KINASE C INHIBITOR PROTEIN-1) (KCIP-1) (FACTOR ACTIVATING EXOENZYME S) (FAS).
2	6.91e+003	12/49 (24%)	27771.3 / 4.73	MOUSE	14-3-3 PROTEIN ZETA/DELTA (PROTEIN KINASE C INHIBITOR PROTEIN-1) (KCIP-1) (MITOCHONDRIAL IMPORT STIMULATION FACTOR S1 SUBUNIT).
3	618	9/49 (18%)	27951.3 / 4.76	HUMAN	14-3-3 PROTEIN BETA/ALPHA (PROTEIN KINASE C INHIBITOR PROTEIN-1) (KCIP-1) (PROTEIN 1054).
4	554	8/49 (16%)	65886.8 / 8.16	HUMAN	KERATIN, TYPE II CYTOSKELETAL 1 (CYTOKERATIN 1) (K1) (CK 1) (67 KD CYTOKERATIN) (HAIR ALPHA PROTEIN).
5	203	5/49 (10%)	20541.8 / 5.55	MOUSE	MAJOR URINARY PROTEIN 4 PRECURSOR (MUP 4).

Detailed Results

1. 13/49 matches (26%). 27745.3 Da, pI = 4.73. Acc. # P29312. HUMAN. 14-3-3 PROTEIN ZETA/DELTA (PROTEIN KINASE C INHIBITOR PROTEIN-1) (KCIP-1) (FACTOR ACTIVATING EXOENZYME S) (FAS)..

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
633.3081	633.3143	-9.6798	76	80	(K)(E)
932.4469	932.4300	18.1414	121	127	(K)(Y)
1189.6936	1189.6615	27.0421	213	222	(K)(D)
1205.6920	1205.6564	29.5783	213	222	(K)DSTLIMQLLR(D)	1Met-ox
1279.6886	1279.6534	27.5154	128	139	(R)(G)
1304.7082	1304.6850	17.7726	104	115	(K)(V)
1548.7587	1548.7142	28.7682	28	41	(K)(N)
1598.7988	1598.8041	-3.3498	116	127	(K)VFYLKMKGDYYR(Y)	1Met-ox
1915.8744	1915.8352	20.4317	12	27	(K)LAEQAERYDDMAACMK(S)	1Cys-am
1931.8781	1931.8301	24.8335	12	27	(K)LAEQAERYDDMAACMK(S)	1Met-ox 1Cys-am
2041.0457	2040.9878	28.3578	140	157	(K)(K)
2132.0063	2131.9923	6.5871	194	212	(K)(D)
2169.1448	2169.0828	28.5950	139	157	(K)(K)
2169.1448	2169.0828	28.5950	140	158	(K)(E)

36 unmatched masses: 601.2831 609.2773 647.2367 713.4089 768.5453 842.5362 868.5552 882.5705 996.6156 1090.5494 1109.5311 1156.6277 1165.6386 1179.6362 1300.5817 1307.6991 1475.8099 1487.7741 1493.7724 1566.7789 1638.8982 1707.8086 1851.9760 1928.9436 1940.9630 2159.0458 2211.1696 2283.2813 2284.2285 2287.1619 2300.2065 2384.0185 2475.3331 2501.3440 2717.0993 2873.4540

The matched peptides cover 48% (119/245 AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

2. 12/49 matches (24%). 27771.3 Da, pI = 4.73. Acc. # P35215. MOUSE. 14-3-3 PROTEIN ZETA/DELTA (PROTEIN KINASE C INHIBITOR PROTEIN-1) (KCIP-1) (MITOCHONDRIAL IMPORT STIMULATION FACTOR S1 SUBUNIT)..

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
633.3081 633.3143 -9.6798 76 80 (K)(E)
932.4469 932.4300 18.1414 121 127 (K)(Y)
1189.6936 1189.6615 27.0421 213 222 (K)(D)
1205.6920 1205.6564 29.5783 213 222 (K)DSTLIMQLLR(D) 1Met-ox
1279.6886 1279.6534 27.5154 128 139 (R)(G)
1548.7587 1548.7142 28.7682 28 41 (K)(N)
1598.7988 1598.8041 -3.3498 116 127 (K)VFYLKMKGDYYR(Y) 1Met-ox
1915.8744 1915.8352 20.4317 12 27 (K)LAEQAERYDDMAACMK(S) 1Cys-am
1931.8781 1931.8301 24.8335 12 27 (K)LAEQAERYDDMAACMK(S) 1Met-ox 1Cys-am
2041.0457 2040.9878 28.3578 140 157 (K)(K)
2132.0063 2131.9923 6.5871 194 212 (K)(D)
2169.1448 2169.0828 28.5950 139 157 (K)(K)
2169.1448 2169.0828 28.5950 140 158 (K)(E)

37 unmatched masses: 601.2831 609.2773 647.2367 713.4089 768.5453 842.5362 868.5552 882.5705 996.6156 1090.5494 1109.5311 1156.6277 1165.6386 1179.6362 1300.5817 1304.7082 1307.6991 1475.8099 1487.7741 1493.7724 1566.7789 1638.8982 1707.8086 1851.9760 1928.9436 1940.9630 2159.0458 2211.1696 2283.2813 2284.2285 2287.1619 2300.2065 2384.0185 2475.3331 2501.3440 2717.0993 2873.4540

The matched peptides cover 43% (107/245 AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

3. 9/49 matches (18%). 27951.3 Da, pI = 4.76. Acc. # P31946. HUMAN. 14-3-3 PROTEIN BETA/ALPHA (PROTEIN KINASE C INHIBITOR PROTEIN-1) (KCIP-1) (PROTEIN 1054)..

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
932.4469 932.4300 18.1414 122 128 (K)MKGDYFR(Y) 1Met-ox
1090.5494 1090.5679 -16.9434 73 81 (R)(E)
1189.6936 1189.6615 27.0421 214 223 (K)(D)
1205.6920 1205.6564 29.5783 214 223 (K)DSTLIMQLLR(D) 1Met-ox
1566.7789 1566.8143 -22.5709 117 128 (K)(Y)
1598.7988 1598.7411 36.0923 29 42 (K)(N)
1940.9630 1940.9612 0.9165 70 84 (K)TERNEKKQQMGKEYR(E) 1Met-ox
2159.0458 2159.0257 9.3440 140 158 (K)(K)
2159.0458 2159.0032 19.7501 195 213 (K)(D)
2287.1619 2287.1206 18.0576 140 159 (K)(E)

40 unmatched masses: 601.2831 609.2773 633.3081 647.2367 713.4089 768.5453 842.5362 868.5552 882.5705 996.6156 1109.5311 1156.6277 1165.6386 1179.6362 1279.6886 1300.5817 1304.7082 1307.6991 1475.8099 1487.7741 1493.7724 1548.7587 1638.8982 1707.8086 1851.9760 1915.8744 1928.9436 1931.8781 2041.0457 2132.0063 2169.1448 2211.1696 2283.2813 2284.2285 2300.2065 2384.0185 2475.3331 2501.3440 2717.0993 2873.4540

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
932.4469	932.4300	18.1414	122	128	(K)MKGDYFR(Y)	1Met-ox
1090.5494	1090.5679	-16.9434	73	81	(R)(E)
1189.6936	1189.6615	27.0421	214	223	(K)(D)
1205.6920	1205.6564	29.5783	214	223	(K)DSTLIMQLLR(D)	1Met-ox
1566.7789	1566.8143	-22.5709	117	128	(K)(Y)
1598.7988	1598.7411	36.0923	29	42	(K)(N)
1940.9630	1940.9612	0.9165	70	84	(K)TERNEKKQQMGKEYR(E)	1Met-ox
2159.0458	2159.0257	9.3440	140	158	(K)(K)
2159.0458	2159.0032	19.7501	195	213	(K)(D)
2287.1619	2287.1206	18.0576	140	159	(K)(E)

The matched peptides cover 36% (90/245 AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

4. 8/49 matches (16%). 65886.8 Da, pI = 8.16. Acc. # P04264. HUMAN. KERATIN, TYPE II CYTOSKELETAL 1 (CYTOKERATIN 1) (K1) (CK 1) (67 KD CYTOKERATIN) (HAIR ALPHA PROTEIN)..

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
1179.6362 1179.6010 29.8217 376 385 (K)(H)
1300.5817 1300.5302 39.6247 257 266 (K)(N)
1475.8099 1475.7858 16.2842 199 210 (R)(W)
1475.8099 1475.7494 40.9402 211 222 (K)(T)
1487.7741 1487.8043 -20.2890 239 250 (R)(L)
1638.8982 1638.8604 23.0789 185 198 (K)(F)
2300.2065 2300.1985 3.4570 343 363 (R)(S)
2384.0185 2383.9524 27.7365 518 548 (R)(G)
2501.3440 2501.2524 36.6154 364 385 (K)(H)

41 unmatched masses: 601.2831 609.2773 633.3081 647.2367 713.4089 768.5453 842.5362 868.5552 882.5705 932.4469 996.6156 1090.5494 1109.5311 1156.6277 1165.6386 1189.6936 1205.6920 1279.6886 1304.7082 1307.6991 1493.7724 1548.7587 1566.7789 1598.7988 1707.8086 1851.9760 1915.8744 1928.9436 1931.8781 1940.9630 2041.0457 2132.0063 2159.0458 2169.1448 2211.1696 2283.2813 2284.2285 2287.1619 2475.3331 2717.0993 2873.4540

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)
1179.6362	1179.6010	29.8217	376	385	(K)(H)
1300.5817	1300.5302	39.6247	257	266	(K)(N)
1475.8099	1475.7858	16.2842	199	210	(R)(W)
1475.8099	1475.7494	40.9402	211	222	(K)(T)
1487.7741	1487.8043	-20.2890	239	250	(R)(L)
1638.8982	1638.8604	23.0789	185	198	(K)(F)
2300.2065	2300.1985	3.4570	343	363	(R)(S)
2384.0185	2383.9524	27.7365	518	548	(R)(G)
2501.3440	2501.2524	36.6154	364	385	(K)(H)

The matched peptides cover 20% (134/643 AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

5. 5/49 matches (10%). 20541.8 Da, pI = 5.55. Acc. # P11590. MOUSE. MAJOR URINARY PROTEIN 4 PRECURSOR (MUP 4)..

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
1090.5494 1090.5791 -27.2436 170 178 (K)(-)
1915.8744 1915.8900 -8.1697 111 125 (K)(D)
1931.8781 1931.8849 -3.5312 111 125 (K)TDYDNYIMFHLINEK(D) 1Met-ox
2475.3331 2475.2884 18.0552 23 44 (K)(R)
2873.4540 2873.5235 -24.1957 129 152 (K)(L)

44 unmatched masses: 601.2831 609.2773 633.3081 647.2367 713.4089 768.5453 842.5362 868.5552 882.5705 932.4469 996.6156 1109.5311 1156.6277 1165.6386 1179.6362 1189.6936 1205.6920 1279.6886 1300.5817 1304.7082 1307.6991 1475.8099 1487.7741 1493.7724 1548.7587 1566.7789 1598.7988 1638.8982 1707.8086 1851.9760 1928.9436 1940.9630 2041.0457 2132.0063 2159.0458 2169.1448 2211.1696 2283.2813 2284.2285 2287.1619 2300.2065 2384.0185 2501.3440 2717.0993

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
1090.5494	1090.5791	-27.2436	170	178	(K)(-)
1915.8744	1915.8900	-8.1697	111	125	(K)(D)
1931.8781	1931.8849	-3.5312	111	125	(K)TDYDNYIMFHLINEK(D)	1Met-ox
2475.3331	2475.2884	18.0552	23	44	(K)(R)
2873.4540	2873.5235	-24.1957	129	152	(K)(L)

The matched peptides cover 38% (69/178 AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):