MS-Fit Search Results

Press stop on your browser if you wish to abort this MS-Fit search prematurely.

Sample ID (comment): HSG059
Database searched: SwissProt.r36
Molecular weight search (1000 - 100000 Da) selects 69977 entries.
Full pI range: 74019 entries.
Species search ( HUMAN MOUSE ) selects 8141 entries.
Combined molecular weight, pI and species searches select 7373 entries.
MS-Fit search selects 672 entries (results displayed for top 5 matches).

Considered modifications: | Oxidation of M | Protein N-terminus Acetylated | Acrylamide Modified Cys |

Min. # Peptides
to Match
4

Peptide Mass
Tolerance (+/-)
50.000 ppm

Peptide Masses
are
monoisotopic

Digest
Used
Trypsin

Max. # Missed
Cleavages
4

Cysteines
Modified by
unmodified

Peptide
N terminus
Hydrogen (H)

Peptide
C terminus
Free Acid (O H)

Input #
Peptide Masses
57

Result Summary

Rank	MOWSE Score	# (%) Masses Matched	Protein MW (Da)/pI	Species	Protein Name
1	3.76e+004	14/57 (24%)	27951.3 / 4.76	HUMAN	14-3-3 PROTEIN BETA/ALPHA (PROTEIN KINASE C INHIBITOR PROTEIN-1) (KCIP-1) (PROTEIN 1054).
2	987	8/57 (14%)	65886.8 / 8.16	HUMAN	KERATIN, TYPE II CYTOSKELETAL 1 (CYTOKERATIN 1) (K1) (CK 1) (67 KD CYTOKERATIN) (HAIR ALPHA PROTEIN).
3	608	7/57 (12%)	27745.3 / 4.73	HUMAN	14-3-3 PROTEIN ZETA/DELTA (PROTEIN KINASE C INHIBITOR PROTEIN-1) (KCIP-1) (FACTOR ACTIVATING EXOENZYME S) (FAS).
3	608	7/57 (12%)	27771.3 / 4.73	MOUSE	14-3-3 PROTEIN ZETA/DELTA (PROTEIN KINASE C INHIBITOR PROTEIN-1) (KCIP-1) (MITOCHONDRIAL IMPORT STIMULATION FACTOR S1 SUBUNIT).
4	374	8/57 (14%)	89614.6 / 8.61	HUMAN	TUMOR NECROSIS FACTOR, ALPHA-INDUCED PROTEIN 3 (PUTATIVE DNA BINDING PROTEIN A20) (ZINC FINGER PROTEIN A20).

Detailed Results

1. 14/57 matches (24%). 27951.3 Da, pI = 4.76. Acc. # P31946. HUMAN. 14-3-3 PROTEIN BETA/ALPHA (PROTEIN KINASE C INHIBITOR PROTEIN-1) (KCIP-1) (PROTEIN 1054)..

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
615.3669	615.3578	14.7821	51	56	(K)(R)
916.4208	916.4351	-15.5994	122	128	(K)(Y)
1108.5623	1108.5573	4.4316	160	168	(K)(L)
1124.5177	1124.5523	-30.7268	160	168	(K)EMQPTHPIR(L)	1Met-ox
1189.6641	1189.6615	2.2308	214	223	(K)(D)
1205.6626	1205.6564	5.1792	214	223	(K)DSTLIMQLLR(D)	1Met-ox
1296.6793	1296.6370	32.5972	77	86	(K)(I)
1566.7729	1566.8143	-26.4303	117	128	(K)(Y)
1598.7461	1598.7411	3.1314	29	42	(K)(N)
1812.8119	1812.8260	-7.8009	13	28	(K)(A)
1940.9351	1940.9612	-13.4424	70	84	(K)TERNEKKQQMGKEYR(E)	1Met-ox
2159.0140	2159.0257	-5.3983	140	158	(K)(K)
2159.0140	2159.0032	5.0077	195	213	(K)(D)
2287.1112	2287.1206	-4.1411	140	159	(K)(E)
2487.1795	2487.2480	-27.5280	29	50	(K)(N)

43 unmatched masses: 619.2664 626.2089 633.2575 639.4321 641.2098 645.2590 647.2445 650.0463 656.0630 666.0298 677.2875 699.3343 724.3247 780.1977 824.4355 825.1181 842.5184 861.0827 864.4784 868.5662 882.5500 1045.5784 1179.5960 1284.5662 1307.6652 1383.6932 1475.7513 1493.7075 1638.8518 1707.7952 1851.9213 1994.0003 2093.1072 2180.9968 2211.1074 2283.1594 2285.1397 2383.9193 2384.9782 2465.2206 2501.2267 2705.1775 2717.1038

The matched peptides cover 53% (131/245 AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

2. 8/57 matches (14%). 65886.8 Da, pI = 8.16. Acc. # P04264. HUMAN. KERATIN, TYPE II CYTOSKELETAL 1 (CYTOKERATIN 1) (K1) (CK 1) (67 KD CYTOKERATIN) (HAIR ALPHA PROTEIN)..

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
1045.5784 1045.5754 2.7964 177 184 (K)(S)
1179.5960 1179.6010 -4.2423 376 385 (K)(H)
1383.6932 1383.6909 1.6760 185 196 (K)(V)
1475.7513 1475.7858 -23.3699 199 210 (R)(W)
1475.7513 1475.7494 1.2851 211 222 (K)(T)
1638.8518 1638.8604 -5.2201 185 198 (K)(F)
1994.0003 1993.9772 11.5770 223 238 (R)(R)
2383.9193 2383.9524 -13.8864 518 548 (R)(G)
2501.2267 2501.2524 -10.2663 364 385 (K)(H)

49 unmatched masses: 615.3669 619.2664 626.2089 633.2575 639.4321 641.2098 645.2590 647.2445 650.0463 656.0630 666.0298 677.2875 699.3343 724.3247 780.1977 824.4355 825.1181 842.5184 861.0827 864.4784 868.5662 882.5500 916.4208 1108.5623 1124.5177 1189.6641 1205.6626 1284.5662 1296.6793 1307.6652 1493.7075 1566.7729 1598.7461 1707.7952 1812.8119 1851.9213 1940.9351 2093.1072 2159.0140 2180.9968 2211.1074 2283.1594 2285.1397 2287.1112 2384.9782 2465.2206 2487.1795 2705.1775 2717.1038

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)
1045.5784	1045.5754	2.7964	177	184	(K)(S)
1179.5960	1179.6010	-4.2423	376	385	(K)(H)
1383.6932	1383.6909	1.6760	185	196	(K)(V)
1475.7513	1475.7858	-23.3699	199	210	(R)(W)
1475.7513	1475.7494	1.2851	211	222	(K)(T)
1638.8518	1638.8604	-5.2201	185	198	(K)(F)
1994.0003	1993.9772	11.5770	223	238	(R)(R)
2383.9193	2383.9524	-13.8864	518	548	(R)(G)
2501.2267	2501.2524	-10.2663	364	385	(K)(H)

The matched peptides cover 17% (115/643 AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

3. 7/57 matches (12%). 27745.3 Da, pI = 4.73. Acc. # P29312. HUMAN. 14-3-3 PROTEIN ZETA/DELTA (PROTEIN KINASE C INHIBITOR PROTEIN-1) (KCIP-1) (FACTOR ACTIVATING EXOENZYME S) (FAS)..

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
615.3669 615.3578 14.7821 50 55 (K)(R)
1108.5623 1108.5573 4.4316 159 167 (K)(L)
1124.5177 1124.5523 -30.7268 159 167 (K)EMQPTHPIR(L) 1Met-ox
1189.6641 1189.6615 2.2308 213 222 (K)(D)
1205.6626 1205.6564 5.1792 213 222 (K)DSTLIMQLLR(D) 1Met-ox
1598.7461 1598.8041 -36.3094 116 127 (K)VFYLKMKGDYYR(Y) 1Met-ox
2705.1775 2705.2868 -40.3802 188 212 (K)(D)

50 unmatched masses: 619.2664 626.2089 633.2575 639.4321 641.2098 645.2590 647.2445 650.0463 656.0630 666.0298 677.2875 699.3343 724.3247 780.1977 824.4355 825.1181 842.5184 861.0827 864.4784 868.5662 882.5500 916.4208 1045.5784 1179.5960 1284.5662 1296.6793 1307.6652 1383.6932 1475.7513 1493.7075 1566.7729 1638.8518 1707.7952 1812.8119 1851.9213 1940.9351 1994.0003 2093.1072 2159.0140 2180.9968 2211.1074 2283.1594 2285.1397 2287.1112 2383.9193 2384.9782 2465.2206 2487.1795 2501.2267 2717.1038

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
615.3669	615.3578	14.7821	50	55	(K)(R)
1108.5623	1108.5573	4.4316	159	167	(K)(L)
1124.5177	1124.5523	-30.7268	159	167	(K)EMQPTHPIR(L)	1Met-ox
1189.6641	1189.6615	2.2308	213	222	(K)(D)
1205.6626	1205.6564	5.1792	213	222	(K)DSTLIMQLLR(D)	1Met-ox
1598.7461	1598.8041	-36.3094	116	127	(K)VFYLKMKGDYYR(Y)	1Met-ox
2705.1775	2705.2868	-40.3802	188	212	(K)(D)

The matched peptides cover 25% (62/245 AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

3. 7/57 matches (12%). 27771.3 Da, pI = 4.73. Acc. # P35215. MOUSE. 14-3-3 PROTEIN ZETA/DELTA (PROTEIN KINASE C INHIBITOR PROTEIN-1) (KCIP-1) (MITOCHONDRIAL IMPORT STIMULATION FACTOR S1 SUBUNIT)..

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
615.3669 615.3578 14.7821 50 55 (K)(R)
1108.5623 1108.5573 4.4316 159 167 (K)(L)
1124.5177 1124.5523 -30.7268 159 167 (K)EMQPTHPIR(L) 1Met-ox
1189.6641 1189.6615 2.2308 213 222 (K)(D)
1205.6626 1205.6564 5.1792 213 222 (K)DSTLIMQLLR(D) 1Met-ox
1598.7461 1598.8041 -36.3094 116 127 (K)VFYLKMKGDYYR(Y) 1Met-ox
2705.1775 2705.2868 -40.3802 188 212 (K)(D)

50 unmatched masses: 619.2664 626.2089 633.2575 639.4321 641.2098 645.2590 647.2445 650.0463 656.0630 666.0298 677.2875 699.3343 724.3247 780.1977 824.4355 825.1181 842.5184 861.0827 864.4784 868.5662 882.5500 916.4208 1045.5784 1179.5960 1284.5662 1296.6793 1307.6652 1383.6932 1475.7513 1493.7075 1566.7729 1638.8518 1707.7952 1812.8119 1851.9213 1940.9351 1994.0003 2093.1072 2159.0140 2180.9968 2211.1074 2283.1594 2285.1397 2287.1112 2383.9193 2384.9782 2465.2206 2487.1795 2501.2267 2717.1038

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
615.3669	615.3578	14.7821	50	55	(K)(R)
1108.5623	1108.5573	4.4316	159	167	(K)(L)
1124.5177	1124.5523	-30.7268	159	167	(K)EMQPTHPIR(L)	1Met-ox
1189.6641	1189.6615	2.2308	213	222	(K)(D)
1205.6626	1205.6564	5.1792	213	222	(K)DSTLIMQLLR(D)	1Met-ox
1598.7461	1598.8041	-36.3094	116	127	(K)VFYLKMKGDYYR(Y)	1Met-ox
2705.1775	2705.2868	-40.3802	188	212	(K)(D)

The matched peptides cover 25% (62/245 AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

4. 8/57 matches (14%). 89614.6 Da, pI = 8.61. Acc. # P21580. HUMAN. TUMOR NECROSIS FACTOR, ALPHA-INDUCED PROTEIN 3 (PUTATIVE DNA BINDING PROTEIN A20) (ZINC FINGER PROTEIN A20)..

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
864.4784 864.4579 23.7169 281 287 (R)(V)
1108.5623 1108.5210 37.2553 677 685 (K)(F)
1179.5960 1179.5581 32.1361 677 685 (K)CFIEAQNQR(F) 1Cys-am
1475.7513 1475.6841 45.5883 288 299 (K)VHFLTDPENEMK(E) 1Met-ox
1493.7075 1493.6921 10.2958 622 632 (K)GFCTLCFIEYR(E) 2Cys-am
1851.9213 1851.9751 -29.0405 421 439 (K)(G)
1994.0003 1994.0500 -24.9175 25 41 (R)(T)
2287.1112 2287.1101 0.4708 542 562 (K)RTTAEASSSLSTSLPPSCHQR(S) 1Cys-am

49 unmatched masses: 615.3669 619.2664 626.2089 633.2575 639.4321 641.2098 645.2590 647.2445 650.0463 656.0630 666.0298 677.2875 699.3343 724.3247 780.1977 824.4355 825.1181 842.5184 861.0827 868.5662 882.5500 916.4208 1045.5784 1124.5177 1189.6641 1205.6626 1284.5662 1296.6793 1307.6652 1383.6932 1566.7729 1598.7461 1638.8518 1707.7952 1812.8119 1940.9351 2093.1072 2159.0140 2180.9968 2211.1074 2283.1594 2285.1397 2383.9193 2384.9782 2465.2206 2487.1795 2501.2267 2705.1775 2717.1038

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
864.4784	864.4579	23.7169	281	287	(R)(V)
1108.5623	1108.5210	37.2553	677	685	(K)(F)
1179.5960	1179.5581	32.1361	677	685	(K)CFIEAQNQR(F)	1Cys-am
1475.7513	1475.6841	45.5883	288	299	(K)VHFLTDPENEMK(E)	1Met-ox
1493.7075	1493.6921	10.2958	622	632	(K)GFCTLCFIEYR(E)	2Cys-am
1851.9213	1851.9751	-29.0405	421	439	(K)(G)
1994.0003	1994.0500	-24.9175	25	41	(R)(T)
2287.1112	2287.1101	0.4708	542	562	(K)RTTAEASSSLSTSLPPSCHQR(S)	1Cys-am

The matched peptides cover 12% (96/790 AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):