MS-Fit Search Results

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Sample ID (comment): HSG061
Database searched: SwissProt.r36
Molecular weight search (1000 - 100000 Da) selects 69977 entries.
Full pI range: 74019 entries.
Species search ( HUMAN MOUSE ) selects 8141 entries.
Combined molecular weight, pI and species searches select 7373 entries.
MS-Fit search selects 1035 entries (results displayed for top 5 matches).

Considered modifications: | Oxidation of M | Protein N-terminus Acetylated | Acrylamide Modified Cys |

Min. # Peptides
to Match
4

Peptide Mass
Tolerance (+/-)
50.000 ppm

Peptide Masses
are
monoisotopic

Digest
Used
Trypsin

Max. # Missed
Cleavages
4

Cysteines
Modified by
unmodified

Peptide
N terminus
Hydrogen (H)

Peptide
C terminus
Free Acid (O H)

Input #
Peptide Masses
58

Result Summary

Rank	MOWSE Score	# (%) Masses Matched	Protein MW (Da)/pI	Species	Protein Name
1	1.57e+003	11/58 (18%)	27764.4 / 4.68	HUMAN	14-3-3 PROTEIN TAU (14-3-3 PROTEIN THETA) (14-3-3 PROTEIN T-CELL) (HS1 PROTEIN).
2	696	7/58 (12%)	7743.1 / 8.72	MOUSE	P8 MTCP-1 PROTEIN (MATURE T-CELL PROLIFERATION-1 TYPE A) (MTCP-1 TYPE A) (P8MTCP1).
3	624	8/58 (13%)	49902.3 / 8.40	HUMAN	DNA PRIMASE SMALL SUBUNIT (EC 2.7.7.-) (DNA PRIMASE 49 KD SUBUNIT) (P49).
4	426	9/58 (15%)	64193.6 / 9.17	HUMAN	ZINC FINGER PROTEIN 74.
4	426	9/58 (15%)	83269.5 / 8.50	HUMAN	ZINC FINGER PROTEIN 184 (FRAGMENT).

Detailed Results

1. 11/58 matches (18%). 27764.4 Da, pI = 4.68. Acc. # P27348. HUMAN. 14-3-3 PROTEIN TAU (14-3-3 PROTEIN THETA) (14-3-3 PROTEIN T-CELL) (HS1 PROTEIN)..

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
1189.6840	1189.6615	18.9205	213	222	(K)(D)
1282.5860	1282.5738	9.5197	128	138	(R)YLAEVACGDDR(K)	1Cys-am
1410.7051	1410.6687	25.7797	128	139	(R)YLAEVACGDDRK(Q)	1Cys-am
1532.7304	1532.7193	7.2906	28	41	(K)(N)
1945.8455	1945.8458	-0.1423	12	27	(K)LAEQAERYDDMATCMK(A)	1Cys-am
1961.8489	1961.8407	4.1535	12	27	(K)LAEQAERYDDMATCMK(A)	1Met-ox 1Cys-am
1977.8282	1977.8356	-3.7330	12	27	(K)LAEQAERYDDMATCMK(A)	2Met-ox 1Cys-am
1977.8282	1977.9129	-42.7956	123	139	(K)(Q)
2014.9245	2014.9358	-5.6019	140	157	(K)(K)
2143.0265	2143.0308	-2.0044	139	157	(R)(K)
2143.0265	2143.0308	-2.0044	140	158	(K)(E)
2195.9377	2195.9854	-21.7246	121	138	(K)MKGDYFRYLAEVACGDDR(K)	1Met-ox 1Cys-am
2271.0714	2271.1257	-23.9201	139	158	(R)(E)

47 unmatched masses: 602.2805 603.1670 614.3490 619.3055 644.3223 679.5286 768.5147 842.5310 882.5460 1045.5845 1109.5307 1117.5370 1283.6035 1296.6825 1367.6805 1548.7175 1566.7326 1643.7958 1915.8193 1929.8566 1931.8484 1932.8215 1940.9276 1944.8774 1948.7791 2011.8983 2038.8842 2040.9398 2041.9766 2049.0863 2082.9603 2093.0703 2109.0616 2122.9452 2153.0832 2169.0683 2209.0570 2211.0943 2215.1693 2231.0738 2233.0503 2234.0916 2265.8877 2283.2063 2284.1480 2299.1441 2465.1741

The matched peptides cover 31% (78/245 AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

2. 7/58 matches (12%). 7743.1 Da, pI = 8.72. Acc. # Q61908. MOUSE. P8 MTCP-1 PROTEIN (MATURE T-CELL PROLIFERATION-1 TYPE A) (MTCP-1 TYPE A) (P8MTCP1)..

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
1282.5860 1282.6102 -18.8489 17 27 (K)(C)
1532.7304 1532.7201 6.7240 5 16 (K)DPCQKQACEIQK(C) 2Cys-am
1945.8455 1945.9298 -43.3116 1 16 (-)MPQKDPCQKQACEIQK(C) 1Cys-am
1961.8489 1961.9247 -38.6640 1 16 (-)MPQKDPCQKQACEIQK(C) 1Met-ox 1Cys-am
2082.9603 2082.9952 -16.7861 10 27 (K)(C)
2215.1693 2215.0705 44.5792 42 60 (R)(L)
2465.1741 2465.2281 -21.8961 17 36 (K)CLQANNYLESKCQAVIQELR(K) 2Cys-am

51 unmatched masses: 602.2805 603.1670 614.3490 619.3055 644.3223 679.5286 768.5147 842.5310 882.5460 1045.5845 1109.5307 1117.5370 1189.6840 1283.6035 1296.6825 1367.6805 1410.7051 1548.7175 1566.7326 1643.7958 1915.8193 1929.8566 1931.8484 1932.8215 1940.9276 1944.8774 1948.7791 1977.8282 2011.8983 2014.9245 2038.8842 2040.9398 2041.9766 2049.0863 2093.0703 2109.0616 2122.9452 2143.0265 2153.0832 2169.0683 2195.9377 2209.0570 2211.0943 2231.0738 2233.0503 2234.0916 2265.8877 2271.0714 2283.2063 2284.1480 2299.1441

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
1282.5860	1282.6102	-18.8489	17	27	(K)(C)
1532.7304	1532.7201	6.7240	5	16	(K)DPCQKQACEIQK(C)	2Cys-am
1945.8455	1945.9298	-43.3116	1	16	(-)MPQKDPCQKQACEIQK(C)	1Cys-am
1961.8489	1961.9247	-38.6640	1	16	(-)MPQKDPCQKQACEIQK(C)	1Met-ox 1Cys-am
2082.9603	2082.9952	-16.7861	10	27	(K)(C)
2215.1693	2215.0705	44.5792	42	60	(R)(L)
2465.1741	2465.2281	-21.8961	17	36	(K)CLQANNYLESKCQAVIQELR(K)	2Cys-am

The matched peptides cover 80% (55/68 AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

3. 8/58 matches (13%). 49902.3 Da, pI = 8.40. Acc. # P49642. HUMAN. DNA PRIMASE SMALL SUBUNIT (EC 2.7.7.-) (DNA PRIMASE 49 KD SUBUNIT) (P49)..

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
614.3490 614.3302 30.5862 15 18 (K)(R)
1296.6825 1296.6267 43.0145 131 140 (K)CWTLMTMAIR(I) 1Cys-am
2049.0863 2049.0630 11.3515 78 95 (K)(L)
2169.0683 2169.1120 -20.1469 1 18 (-)ETFDPTELPELLKLYYR(R) Acet N
2209.0570 2209.1671 -49.8030 145 162 (R)(R)
2231.0738 2231.0458 12.5504 288 304 (K)(L)
2233.0503 2232.9770 32.8236 121 140 (R)(I)
2234.0916 2234.0802 5.0999 368 385 (K)(T)

50 unmatched masses: 602.2805 603.1670 619.3055 644.3223 679.5286 768.5147 842.5310 882.5460 1045.5845 1109.5307 1117.5370 1189.6840 1282.5860 1283.6035 1367.6805 1410.7051 1532.7304 1548.7175 1566.7326 1643.7958 1915.8193 1929.8566 1931.8484 1932.8215 1940.9276 1944.8774 1945.8455 1948.7791 1961.8489 1977.8282 2011.8983 2014.9245 2038.8842 2040.9398 2041.9766 2082.9603 2093.0703 2109.0616 2122.9452 2143.0265 2153.0832 2195.9377 2211.0943 2215.1693 2265.8877 2271.0714 2283.2063 2284.1480 2299.1441 2465.1741

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
614.3490	614.3302	30.5862	15	18	(K)(R)
1296.6825	1296.6267	43.0145	131	140	(K)CWTLMTMAIR(I)	1Cys-am
2049.0863	2049.0630	11.3515	78	95	(K)(L)
2169.0683	2169.1120	-20.1469	1	18	(-)ETFDPTELPELLKLYYR(R)	Acet N
2209.0570	2209.1671	-49.8030	145	162	(R)(R)
2231.0738	2231.0458	12.5504	288	304	(K)(L)
2233.0503	2232.9770	32.8236	121	140	(R)(I)
2234.0916	2234.0802	5.0999	368	385	(K)(T)

The matched peptides cover 25% (109/420 AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

4. 9/58 matches (15%). 64193.6 Da, pI = 9.17. Acc. # Q16587. HUMAN. ZINC FINGER PROTEIN 74..

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
1283.6035 1283.5625 31.9571 290 300 (R)CGECGKAFNQR(T) 1Cys-am
1410.7051 1410.7566 -36.4893 81 94 (R)(R)
1532.7304 1532.6903 26.2064 145 159 (R)(F)
1532.7304 1532.7102 13.1806 436 448 (K)AFSCHSSLIMHQR(I) 1Met-ox
1915.8193 1915.8431 -12.4299 168 186 (R)GAGAGEGEFVCGECGKAFR(Q) 1Cys-am
1961.8489 1961.8387 5.1900 489 504 (K)(L)
1977.8282 1977.8336 -2.7048 489 504 (K)CGEMFNWSSHLTEHQR(L) 1Met-ox
2109.0616 2108.9871 35.3427 306 323 (R)HHRIHTGEKPYQCGSCGK(A) 1Cys-am
2231.0738 2231.0767 -1.2738 145 164 (R)LCAGENASTPSEPEKFPQVR(R) 1Cys-am
2231.0738 2231.0081 29.4441 262 280 (K)CSACEKAFSCSSLLSMHLR(V) 1Met-ox 2Cys-am
2271.0714 2271.0399 13.8685 166 186 (R)QRGAGAGEGEFVCGECGKAFR(Q) 2Cys-am

49 unmatched masses: 602.2805 603.1670 614.3490 619.3055 644.3223 679.5286 768.5147 842.5310 882.5460 1045.5845 1109.5307 1117.5370 1189.6840 1282.5860 1296.6825 1367.6805 1548.7175 1566.7326 1643.7958 1929.8566 1931.8484 1932.8215 1940.9276 1944.8774 1945.8455 1948.7791 2011.8983 2014.9245 2038.8842 2040.9398 2041.9766 2049.0863 2082.9603 2093.0703 2122.9452 2143.0265 2153.0832 2169.0683 2195.9377 2209.0570 2211.0943 2215.1693 2233.0503 2234.0916 2265.8877 2283.2063 2284.1480 2299.1441 2465.1741

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
1283.6035	1283.5625	31.9571	290	300	(R)CGECGKAFNQR(T)	1Cys-am
1410.7051	1410.7566	-36.4893	81	94	(R)(R)
1532.7304	1532.6903	26.2064	145	159	(R)(F)
1532.7304	1532.7102	13.1806	436	448	(K)AFSCHSSLIMHQR(I)	1Met-ox
1915.8193	1915.8431	-12.4299	168	186	(R)GAGAGEGEFVCGECGKAFR(Q)	1Cys-am
1961.8489	1961.8387	5.1900	489	504	(K)(L)
1977.8282	1977.8336	-2.7048	489	504	(K)CGEMFNWSSHLTEHQR(L)	1Met-ox
2109.0616	2108.9871	35.3427	306	323	(R)HHRIHTGEKPYQCGSCGK(A)	1Cys-am
2231.0738	2231.0767	-1.2738	145	164	(R)LCAGENASTPSEPEKFPQVR(R)	1Cys-am
2231.0738	2231.0081	29.4441	262	280	(K)CSACEKAFSCSSLLSMHLR(V)	1Met-ox 2Cys-am
2271.0714	2271.0399	13.8685	166	186	(R)QRGAGAGEGEFVCGECGKAFR(Q)	2Cys-am

The matched peptides cover 23% (132/572 AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

4. 9/58 matches (15%). 83269.5 Da, pI = 8.50. Acc. # Q99676. HUMAN. ZINC FINGER PROTEIN 184 (FRAGMENT)..

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
1109.5307 1109.5744 -39.3435 373 382 (K)(H)
1548.7175 1548.7117 3.7355 610 621 (K)THTEEKPYQCNK(C) 1Cys-am
1961.8489 1961.9438 -48.3968 553 568 (R)KIHTGERPYKCNECGR(A) 1Cys-am
1977.8282 1977.8985 -35.5216 199 214 (K)CNECGKAFSYCSALIR(H) 3Cys-am
2082.9603 2082.9233 17.7334 196 214 (K)(H)
2122.9452 2123.0166 -33.6547 582 599 (R)IHTGAKPYECAECGKAFR(H) 2Cys-am
2233.0503 2233.0836 -14.8929 161 181 (K)(R)
2271.0714 2271.0763 -2.1530 271 288 (R)HQRIHTGEKPYKCDECGK(A) 2Cys-am
2271.0714 2270.9745 42.6571 383 400 (R)HHMIHTGEKPYECNECGK(A) 1Met-ox 2Cys-am
2299.1441 2299.0600 36.5819 218 236 (R)THTGEKPYKCNECEKAFSR(S) 1Cys-am

49 unmatched masses: 602.2805 603.1670 614.3490 619.3055 644.3223 679.5286 768.5147 842.5310 882.5460 1045.5845 1117.5370 1189.6840 1282.5860 1283.6035 1296.6825 1367.6805 1410.7051 1532.7304 1566.7326 1643.7958 1915.8193 1929.8566 1931.8484 1932.8215 1940.9276 1944.8774 1945.8455 1948.7791 2011.8983 2014.9245 2038.8842 2040.9398 2041.9766 2049.0863 2093.0703 2109.0616 2143.0265 2153.0832 2169.0683 2195.9377 2209.0570 2211.0943 2215.1693 2231.0738 2234.0916 2265.8877 2283.2063 2284.1480 2465.1741

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
1109.5307	1109.5744	-39.3435	373	382	(K)(H)
1548.7175	1548.7117	3.7355	610	621	(K)THTEEKPYQCNK(C)	1Cys-am
1961.8489	1961.9438	-48.3968	553	568	(R)KIHTGERPYKCNECGR(A)	1Cys-am
1977.8282	1977.8985	-35.5216	199	214	(K)CNECGKAFSYCSALIR(H)	3Cys-am
2082.9603	2082.9233	17.7334	196	214	(K)(H)
2122.9452	2123.0166	-33.6547	582	599	(R)IHTGAKPYECAECGKAFR(H)	2Cys-am
2233.0503	2233.0836	-14.8929	161	181	(K)(R)
2271.0714	2271.0763	-2.1530	271	288	(R)HQRIHTGEKPYKCDECGK(A)	2Cys-am
2271.0714	2270.9745	42.6571	383	400	(R)HHMIHTGEKPYECNECGK(A)	1Met-ox 2Cys-am
2299.1441	2299.0600	36.5819	218	236	(R)THTGEKPYKCNECEKAFSR(S)	1Cys-am

The matched peptides cover 20% (151/726 AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):