MS-Fit Search Results

Press stop on your browser if you wish to abort this MS-Fit search prematurely.
Sample ID (comment): HSG062
Database searched: SwissProt.12.15.2000
Molecular weight search (1000 - 100000 Da) selects 86001 entries.
Full pI range: 90939 entries.
Species search ( HUMAN MOUSE ) selects 10164 entries.
Combined molecular weight, pI and species searches select 9152 entries.
Pre searches select 9152 entries.
MS-Fit search selects 473 entries (results displayed for top 5 matches).

Parameters Used in Search

Considered modifications: | Oxidation of M | Protein N-terminus Acetylated | Acrylamide Modified Cys |

4	50.000 ppm	monoisotopic	Trypsin	3	carbamidomethylation	Hydrogen (H)	Free Acid (O H)
Min. # Peptides to Match	Peptide Mass Tolerance (+/-)	Peptide Masses are	Digest Used	Max. # Missed Cleavages	Cysteines Modified by	Peptide N terminus	Peptide C terminus

Result Summary

Rank	MOWSE Score	# (%) Masses Matched	Protein MW (Da)/pI	Species	Protein Name
1	2.28e+010	23/30 (76%)	61055.2 / 5.70	HUMAN	60 KDA HEAT SHOCK PROTEIN, MITOCHONDRIAL PRECURSOR (HSP60) (60 KDA CHAPERONIN) (CPN60) (HEAT SHOCK PROTEIN 60) (HSP-60) (MITOCHONDRIAL MATRIX PROTEIN P1) (P60 LYMPHOCYTE PROTEIN) (HUCHA60)
2	9.7e+005	12/30 (40%)	60956.0 / 5.91	MOUSE	60 KDA HEAT SHOCK PROTEIN, MITOCHONDRIAL PRECURSOR (HSP60) (60 KDA CHAPERONIN) (CPN60) (HEAT SHOCK PROTEIN 60) (HSP-60) (MITOCHONDRIAL MATRIX PROTEIN P1) (HSP-65)
3	1.73e+003	6/30 (20%)	57878.3 / 7.58	HUMAN	PYRUVATE KINASE, M1 ISOZYME (PYRUVATE KINASE MUSCLE ISOZYME) (CYTOSOLIC THYROID HORMONE-BINDING PROTEIN) (CTHBP) (THBP1)
4	1.73e+003	6/30 (20%)	57914.4 / 7.95	HUMAN	PYRUVATE KINASE, M2 ISOZYME
5	1.43e+003	8/30 (26%)	57887.5 / 7.17	MOUSE	PYRUVATE KINASE, M2 ISOZYME

Detailed Results

1. 23/30 matches (76%). 61055.2 Da, pI = 5.70. Acc. # P10809. HUMAN. 60 KDA HEAT SHOCK PROTEIN, MITOCHONDRIAL PRECURSOR (HSP60) (60 KDA CHAPERONIN) (CPN60) (HEAT SHOCK PROTEIN 60) (HSP-60) (MITOCHONDRIAL MATRIX PROTEIN P1) (P60 LYMPHOCYTE PROTEIN) (HUCHA60).

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
833.4059	833.3906	18.3141	302	309	(K)(K)
855.4910	855.4688	25.8729	134	141	(K)(R)
960.5306	960.5114	19.9585	421	429	(R)(A)
1344.7345	1344.7163	13.5010	61	72	(R)(V)
1389.7029	1389.7054	-1.8486	222	233	(R)(G)
1615.7790	1615.7678	6.9383	237	249	(K)CEFQDAYVLLSEK(K)	1Cys-am
1698.9237	1698.9213	1.4331	430	446	(R)AAVEEGIVLGGGCALLR(C)	1Cys-am
1785.8538	1785.8693	-8.6648	447	462	(R)CIPALDSLTPANEDQK(I)	1Cys-am
1859.9004	1859.9901	-48.1981	353	369	(K)(R)
1875.9323	1875.9850	-28.0667	353	369	(K)DDAMLLKGKGDKAQIEK(R)	1Met-ox
1919.0937	1919.0714	11.5879	251	268	(K)(K)
1938.9497	1938.9483	0.7639	206	221	(K)TLNDELEIIEGMKFDR(G)	1Met-ox
2038.0160	2038.0232	-3.5289	371	387	(R)(E)
2047.1712	2047.1664	2.3648	250	268	(K)(K)
2113.1144	2113.1401	-12.1426	38	58	(R)(G)
2129.1528	2129.1350	8.3265	38	58	(R)ALMLQGVDLLADAVAVTMGPK(G)	1Met-ox
2194.1252	2194.1243	0.3824	370	387	(K)(E)
2295.1517	2295.1608	-3.9300	371	389	(R)(L)
2365.3581	2365.3343	10.0700	269	290	(R)(L)
2508.0528	2508.1097	-22.6562	494	516	(K)(G)
2524.0961	2524.1046	-3.3754	494	516	(K)IMQSSSEVGYDAMAGDFVNMVEK(G)	1Met-ox
2560.1990	2560.2491	-19.5514	97	121	(K)(S)
3097.4971	3097.5152	-5.8379	315	344	(K)(V)

Click link below to search for another component.

Click individual mass to do a non-specific cleavage search.

The matched peptides cover 50% (288/573AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 76.7 Mean Error = -1.6647 ppm Data Tolerance = 33.5448 ppm Mean Missed Cleavages = 0.4

2. 12/30 matches (40%). 60956.0 Da, pI = 5.91. Acc. # P19226. MOUSE. 60 KDA HEAT SHOCK PROTEIN, MITOCHONDRIAL PRECURSOR (HSP60) (60 KDA CHAPERONIN) (CPN60) (HEAT SHOCK PROTEIN 60) (HSP-60) (MITOCHONDRIAL MATRIX PROTEIN P1) (HSP-65).

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
833.4059 833.3906 18.3141 302 309 (K)(K)
855.4910 855.4688 25.8729 134 141 (K)(R)
960.5306 960.5114 19.9585 421 429 (R)(A)
1344.7345 1344.7163 13.5010 61 72 (R)(V)
1389.7029 1389.7054 -1.8486 222 233 (R)(G)
1615.7790 1615.7678 6.9383 237 249 (K)CEFQDAYVLLSEK(K) 1Cys-am
1698.9237 1698.9213 1.4331 430 446 (R)AAVEEGIVLGGGCALLR(C) 1Cys-am
1938.9497 1938.9483 0.7639 206 221 (K)TLNDELEIIEGMKFDR(G) 1Met-ox
2113.1144 2113.1401 -12.1426 38 58 (R)(G)
2129.1528 2129.1350 8.3265 38 58 (R)ALMLQGVDLLADAVAVTMGPK(G) 1Met-ox
2365.3581 2365.3343 10.0700 269 290 (R)(L)
2560.1990 2560.2491 -19.5514 97 121 (K)(S)

Click link below to search for another component.

Click individual mass to do a non-specific cleavage search.

The matched peptides cover 28% (163/573AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 40.0 Mean Error = 5.9696 ppm Data Tolerance = 26.3709 ppm Mean Missed Cleavages = 0.1

3. 6/30 matches (20%). 57878.3 Da, pI = 7.58. Acc. # P14618. HUMAN. PYRUVATE KINASE, M1 ISOZYME (PYRUVATE KINASE MUSCLE ISOZYME) (CYTOSOLIC THYROID HORMONE-BINDING PROTEIN) (CTHBP) (THBP1).

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
1859.9004 1859.9002 0.1234 231 246 (K)(K)
1875.9323 1875.8951 19.8438 231 246 (K)FGVEQDVDMVFASFIR(K) 1Met-ox
1931.9543 1931.9867 -16.7668 384 399 (R)(R)
2465.2771 2465.2928 -6.3502 93 115 (R)(G)
2508.0528 2508.1598 -42.6390 343 367 (R)AEGSDVANAVLDGADCIMLSGETAK(G) 1Cys-am
2524.0961 2524.1547 -23.2320 343 367 (R)AEGSDVANAVLDGADCIMLSGETAK(G) 1Met-ox 1Cys-am

Click link below to search for another component.

Click individual mass to do a non-specific cleavage search.

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
1859.9004	1859.9002	0.1234	231	246	(K)(K)
1875.9323	1875.8951	19.8438	231	246	(K)FGVEQDVDMVFASFIR(K)	1Met-ox
1931.9543	1931.9867	-16.7668	384	399	(R)(R)
2465.2771	2465.2928	-6.3502	93	115	(R)(G)
2508.0528	2508.1598	-42.6390	343	367	(R)AEGSDVANAVLDGADCIMLSGETAK(G)	1Cys-am
2524.0961	2524.1547	-23.2320	343	367	(R)AEGSDVANAVLDGADCIMLSGETAK(G)	1Met-ox 1Cys-am

The matched peptides cover 15% (80/531AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 20.0 Mean Error = -11.5035 ppm Data Tolerance = 42.6997 ppm Mean Missed Cleavages = 0.0

4. 6/30 matches (20%). 57914.4 Da, pI = 7.95. Acc. # P14786. HUMAN. PYRUVATE KINASE, M2 ISOZYME.

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
1859.9004 1859.9002 0.1234 231 246 (K)(K)
1875.9323 1875.8951 19.8438 231 246 (K)FGVEQDVDMVFASFIR(K) 1Met-ox
1931.9543 1931.9867 -16.7668 384 399 (R)(R)
2465.2771 2465.2928 -6.3502 93 115 (R)(G)
2508.0528 2508.1598 -42.6390 343 367 (R)AEGSDVANAVLDGADCIMLSGETAK(G) 1Cys-am
2524.0961 2524.1547 -23.2320 343 367 (R)AEGSDVANAVLDGADCIMLSGETAK(G) 1Met-ox 1Cys-am

Click link below to search for another component.

Click individual mass to do a non-specific cleavage search.

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
1859.9004	1859.9002	0.1234	231	246	(K)(K)
1875.9323	1875.8951	19.8438	231	246	(K)FGVEQDVDMVFASFIR(K)	1Met-ox
1931.9543	1931.9867	-16.7668	384	399	(R)(R)
2465.2771	2465.2928	-6.3502	93	115	(R)(G)
2508.0528	2508.1598	-42.6390	343	367	(R)AEGSDVANAVLDGADCIMLSGETAK(G)	1Cys-am
2524.0961	2524.1547	-23.2320	343	367	(R)AEGSDVANAVLDGADCIMLSGETAK(G)	1Met-ox 1Cys-am

The matched peptides cover 15% (80/531AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 20.0 Mean Error = -11.5035 ppm Data Tolerance = 42.6997 ppm Mean Missed Cleavages = 0.0

5. 8/30 matches (26%). 57887.5 Da, pI = 7.17. Acc. # P52480. MOUSE. PYRUVATE KINASE, M2 ISOZYME.

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
1859.9004 1859.9002 0.1234 231 246 (K)(K)
1875.9323 1875.8951 19.8438 231 246 (K)FGVEQDVDMVFASFIR(K) 1Met-ox
1919.0937 1919.0168 40.0472 320 336 (R)(K)
1931.9543 1931.9867 -16.7668 384 399 (R)(R)
1938.9497 1938.9815 -16.3746 57 73 (R)SVEMLKEMIKSGMNVAR(L) 1Met-ox
2508.0528 2508.1598 -42.6390 343 367 (R)AEGSDVANAVLDGADCIMLSGETAK(G) 1Cys-am
2524.0961 2524.1547 -23.2320 343 367 (R)AEGSDVANAVLDGADCIMLSGETAK(G) 1Met-ox 1Cys-am
3098.5586 3098.6575 -31.9222 317 342 (R)CNRAGKPVICSTQMLEIMIKKPRPTR(A) 1Cys-am

Click link below to search for another component.

Click individual mass to do a non-specific cleavage search.

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
1859.9004	1859.9002	0.1234	231	246	(K)(K)
1875.9323	1875.8951	19.8438	231	246	(K)FGVEQDVDMVFASFIR(K)	1Met-ox
1919.0937	1919.0168	40.0472	320	336	(R)(K)
1931.9543	1931.9867	-16.7668	384	399	(R)(R)
1938.9497	1938.9815	-16.3746	57	73	(R)SVEMLKEMIKSGMNVAR(L)	1Met-ox
2508.0528	2508.1598	-42.6390	343	367	(R)AEGSDVANAVLDGADCIMLSGETAK(G)	1Cys-am
2524.0961	2524.1547	-23.2320	343	367	(R)AEGSDVANAVLDGADCIMLSGETAK(G)	1Met-ox 1Cys-am
3098.5586	3098.6575	-31.9222	317	342	(R)CNRAGKPVICSTQMLEIMIKKPRPTR(A)	1Cys-am

The matched peptides cover 18% (100/531AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 26.7 Mean Error = -8.8650 ppm Data Tolerance = 55.0062 ppm Mean Missed Cleavages = 0.5