MS-Fit Search Results

Press stop on your browser if you wish to abort this MS-Fit search prematurely.
Sample ID (comment): HSG063
Database searched: SwissProt.12.15.2000
Molecular weight search (1000 - 100000 Da) selects 86001 entries.
Full pI range: 90939 entries.
Species search ( HUMAN MOUSE ) selects 10164 entries.
Combined molecular weight, pI and species searches select 9152 entries.
Pre searches select 9152 entries.
MS-Fit search selects 348 entries (results displayed for top 5 matches).

Parameters Used in Search

Considered modifications: | Oxidation of M | Protein N-terminus Acetylated | Acrylamide Modified Cys |

4	50.000 ppm	monoisotopic	Trypsin	3	carbamidomethylation	Hydrogen (H)	Free Acid (O H)
Min. # Peptides to Match	Peptide Mass Tolerance (+/-)	Peptide Masses are	Digest Used	Max. # Missed Cleavages	Cysteines Modified by	Peptide N terminus	Peptide C terminus

Result Summary

Rank	MOWSE Score	# (%) Masses Matched	Protein MW (Da)/pI	Species	Protein Name
1	1.63e+006	14/32 (43%)	53674.7 / 5.52	HUMAN	KERATIN, TYPE II CYTOSKELETAL 8 (CYTOKERATIN 8) (K8) (CK 8)
2	3.19e+005	12/32 (37%)	54449.6 / 5.50	MOUSE	KERATIN, TYPE II CYTOSKELETAL 8 (CYTOKERATIN 8) (CYTOKERATIN ENDO A)
3	3.55e+003	8/32 (25%)	66018.2 / 8.16	HUMAN	KERATIN, TYPE II CYTOSKELETAL 1 (CYTOKERATIN 1) (K1) (CK 1) (67 KDA CYTOKERATIN) (HAIR ALPHA PROTEIN)
4	791	5/32 (15%)	59519.1 / 5.13	HUMAN	KERATIN, TYPE I CYTOSKELETAL 10 (CYTOKERATIN 10) (K10) (CK 10)
5	664	10/32 (31%)	68719.1 / 9.48	HUMAN	ZINC FINGER PROTEIN 85 (ZINC FINGER PROTEIN HPF4) (HTF1)

Detailed Results

1. 14/32 matches (43%). 53674.7 Da, pI = 5.52. Acc. # P05787. HUMAN. KERATIN, TYPE II CYTOSKELETAL 8 (CYTOKERATIN 8) (K8) (CK 8).

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
1079.5163	1079.5373	-19.4649	226	233	(R)(E)
1079.5163	1079.5122	3.8342	265	273	(K)(S)
1129.6141	1129.6217	-6.7114	342	352	(R)(L)
1129.6141	1129.6217	-6.7114	353	362	(K)(A)
1137.5747	1137.5792	-3.9293	286	295	(K)(H)
1277.6985	1277.7105	-9.3780	382	392	(K)(K)
1344.6502	1344.6759	-19.1182	329	341	(R)(G)
1352.6647	1352.6772	-9.2146	187	197	(R)(K)
1419.7347	1419.7484	-9.6436	214	225	(R)(Q)
1475.7536	1475.6987	37.2177	402	414	(R)(T)
1847.7482	1847.8056	-31.0775	134	148	(R)(R)
1863.7478	1863.8006	-28.3189	134	148	(R)SNMDNMFESYINNLR(R)	1Met-ox
1879.7034	1879.7955	-48.9731	134	148	(R)SNMDNMFESYINNLR(R)	2Met-ox
2034.0042	2034.0582	-26.5325	159	176	(K)(N)
2108.9888	2109.0134	-11.6786	234	252	(R)(S)
2367.2276	2367.1913	15.3436	363	381	(R)AKQDMARQLREYQELMNVK(L)	1Met-ox

Click link below to search for another component.

Click individual mass to do a non-specific cleavage search.

The matched peptides cover 37% (179/483AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 43.8 Mean Error = -10.8973 ppm Data Tolerance = 39.5223 ppm Mean Missed Cleavages = 0.3

2. 12/32 matches (37%). 54449.6 Da, pI = 5.50. Acc. # P11679. MOUSE. KERATIN, TYPE II CYTOSKELETAL 8 (CYTOKERATIN 8) (CYTOKERATIN ENDO A).

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
1079.5163 1079.5122 3.8342 271 279 (R)(S)
1344.6502 1344.6759 -19.1182 334 346 (R)(G)
1352.6647 1352.6772 -9.2146 193 203 (R)(K)
1383.6929 1383.7272 -24.8207 103 114 (K)(V)
1419.7347 1419.7484 -9.6436 220 231 (R)(Q)
1475.7536 1475.6987 37.2177 407 419 (R)(T)
1796.9312 1796.9758 -24.8302 353 369 (K)(Q)
1847.7482 1847.8056 -31.0775 140 154 (R)(R)
1863.7478 1863.8006 -28.3189 140 154 (R)SNMDNMFESYINNLR(R) 1Met-ox
1879.7034 1879.7955 -48.9731 140 154 (R)SNMDNMFESYINNLR(R) 2Met-ox
2034.0042 2034.0582 -26.5325 165 182 (K)(N)
2108.9888 2109.0134 -11.6786 240 258 (R)(S)

Click link below to search for another component.

Click individual mass to do a non-specific cleavage search.

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
1079.5163	1079.5122	3.8342	271	279	(R)(S)
1344.6502	1344.6759	-19.1182	334	346	(R)(G)
1352.6647	1352.6772	-9.2146	193	203	(R)(K)
1383.6929	1383.7272	-24.8207	103	114	(K)(V)
1419.7347	1419.7484	-9.6436	220	231	(R)(Q)
1475.7536	1475.6987	37.2177	407	419	(R)(T)
1796.9312	1796.9758	-24.8302	353	369	(K)(Q)
1847.7482	1847.8056	-31.0775	140	154	(R)(R)
1863.7478	1863.8006	-28.3189	140	154	(R)SNMDNMFESYINNLR(R)	1Met-ox
1879.7034	1879.7955	-48.9731	140	154	(R)SNMDNMFESYINNLR(R)	2Met-ox
2034.0042	2034.0582	-26.5325	165	182	(K)(N)
2108.9888	2109.0134	-11.6786	240	258	(R)(S)

The matched peptides cover 28% (139/489AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 37.5 Mean Error = -16.0963 ppm Data Tolerance = 42.9514 ppm Mean Missed Cleavages = 0.3

3. 8/32 matches (25%). 66018.2 Da, pI = 8.16. Acc. # P04264. HUMAN. KERATIN, TYPE II CYTOSKELETAL 1 (CYTOKERATIN 1) (K1) (CK 1) (67 KDA CYTOKERATIN) (HAIR ALPHA PROTEIN).

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
1179.5704 1179.6010 -25.9324 377 386 (K)(H)
1277.6985 1277.7105 -9.3780 473 483 (K)(T)
1383.6929 1383.6909 1.4746 186 197 (K)(V)
1475.7536 1475.7858 -21.8437 200 211 (R)(W)
1475.7536 1475.7494 2.8114 212 223 (K)(T)
1487.7327 1487.8043 -48.1089 240 251 (R)(L)
1993.9384 1993.9772 -19.4396 224 239 (R)(R)
2150.0727 2150.0783 -2.6326 224 240 (R)(R)
2383.9202 2383.9524 -13.5003 519 549 (R)(G)

Click link below to search for another component.

Click individual mass to do a non-specific cleavage search.

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)
1179.5704	1179.6010	-25.9324	377	386	(K)(H)
1277.6985	1277.7105	-9.3780	473	483	(K)(T)
1383.6929	1383.6909	1.4746	186	197	(K)(V)
1475.7536	1475.7858	-21.8437	200	211	(R)(W)
1475.7536	1475.7494	2.8114	212	223	(K)(T)
1487.7327	1487.8043	-48.1089	240	251	(R)(L)
1993.9384	1993.9772	-19.4396	224	239	(R)(R)
2150.0727	2150.0783	-2.6326	224	240	(R)(R)
2383.9202	2383.9524	-13.5003	519	549	(R)(G)

The matched peptides cover 18% (116/644AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 25.0 Mean Error = -15.1722 ppm Data Tolerance = 32.0739 ppm Mean Missed Cleavages = 0.4

4. 5/32 matches (15%). 59519.1 Da, pI = 5.13. Acc. # P13645. HUMAN. KERATIN, TYPE I CYTOSKELETAL 10 (CYTOKERATIN 10) (K10) (CK 10).

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
1796.9312 1797.0122 -45.0774 371 386 (R)(Q)
2095.9974 2096.0473 -23.8111 267 284 (K)(K)
2212.0779 2212.0985 -9.3292 344 362 (K)(S)
2367.2276 2367.2632 -15.0306 208 228 (K)(L)
2872.3267 2872.3933 -23.1861 296 322 (R)(S)

Click link below to search for another component.

Click individual mass to do a non-specific cleavage search.

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)
1796.9312	1797.0122	-45.0774	371	386	(R)(Q)
2095.9974	2096.0473	-23.8111	267	284	(K)(K)
2212.0779	2212.0985	-9.3292	344	362	(K)(S)
2367.2276	2367.2632	-15.0306	208	228	(K)(L)
2872.3267	2872.3933	-23.1861	296	322	(R)(S)

The matched peptides cover 17% (101/593AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 15.6 Mean Error = -23.2869 ppm Data Tolerance = 27.1679 ppm Mean Missed Cleavages = 0.2

5. 10/32 matches (31%). 68719.1 Da, pI = 9.48. Acc. # Q03923. HUMAN. ZINC FINGER PROTEIN 85 (ZINC FINGER PROTEIN HPF4) (HTF1).

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
1137.5747 1137.5653 8.3062 462 471 (K)(H)
1419.7347 1419.7385 -2.6733 210 221 (K)(R)
1487.7327 1487.8044 -48.2318 278 289 (K)IIHTGEKPYKCK(E) 1Cys-am
1487.7327 1487.8044 -48.2318 334 345 (K)IIHTGEKPYKCK(K) 1Cys-am
1487.7327 1487.8044 -48.2318 390 401 (K)IIHTGEKPYKCK(E) 1Cys-am
1708.7201 1708.7747 -31.9184 129 144 (K)(I)
1893.8716 1893.8475 12.7070 559 573 (R)(A)
2150.0727 2150.0123 28.0785 232 249 (K)(K)
2211.0073 2211.0327 -11.4846 204 221 (K)CEECGKAFNWSSTLTKHK(R) 2Cys-am
2212.0779 2212.1548 -34.8018 416 433 (K)(A)
2367.2276 2367.1338 39.6412 204 222 (K)CEECGKAFNWSSTLTKHKR(I) 2Cys-am
2872.3267 2872.3623 -12.3850 17 38 (K)(N)

Click link below to search for another component.

Click individual mass to do a non-specific cleavage search.

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
1137.5747	1137.5653	8.3062	462	471	(K)(H)
1419.7347	1419.7385	-2.6733	210	221	(K)(R)
1487.7327	1487.8044	-48.2318	278	289	(K)IIHTGEKPYKCK(E)	1Cys-am
1487.7327	1487.8044	-48.2318	334	345	(K)IIHTGEKPYKCK(K)	1Cys-am
1487.7327	1487.8044	-48.2318	390	401	(K)IIHTGEKPYKCK(E)	1Cys-am
1708.7201	1708.7747	-31.9184	129	144	(K)(I)
1893.8716	1893.8475	12.7070	559	573	(R)(A)
2150.0727	2150.0123	28.0785	232	249	(K)(K)
2211.0073	2211.0327	-11.4846	204	221	(K)CEECGKAFNWSSTLTKHK(R)	2Cys-am
2212.0779	2212.1548	-34.8018	416	433	(K)(A)
2367.2276	2367.1338	39.6412	204	222	(K)CEECGKAFNWSSTLTKHKR(I)	2Cys-am
2872.3267	2872.3623	-12.3850	17	38	(K)(N)

The matched peptides cover 25% (154/595AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 31.3 Mean Error = -12.4355 ppm Data Tolerance = 61.0094 ppm Mean Missed Cleavages = 1.4