MS-Fit Search Results

Press stop on your browser if you wish to abort this MS-Fit search prematurely.
Sample ID (comment): HSG064
Database searched: SwissProt.7.2.2001
Molecular weight search (1000 - 100000 Da) selects 93834 entries.
Full pI range: 99359 entries.
Species search ( HUMAN MOUSE ) selects 11676 entries.
Combined molecular weight, pI and species searches select 10399 entries.
Pre searches select 10399 entries.
MS-Fit search selects 318 entries (results displayed for top 5 matches).

Parameters Used in Search

Considered modifications: | Peptide N-terminal Gln to pyroGlu | Oxidation of M | Protein N-terminus Acetylated |

4	50.000 ppm	monoisotopic	Trypsin	3	acrylamide	Hydrogen (H)	Free Acid (O H)
Min. # Peptides to Match	Peptide Mass Tolerance (+/-)	Peptide Masses are	Digest Used	Max. # Missed Cleavages	Cysteines Modified by	Peptide N terminus	Peptide C terminus

Result Summary

Rank	MOWSE Score	# (%) Masses Matched	Protein MW (Da)/pI	Species	Protein Name
1	1.69e+004	10/80 (12%)	32575.3 / 4.64	HUMAN	NUCLEOPHOSMIN (NPM) (NUCLEOLAR PHOSPHOPROTEIN B23) (NUMATRIN) (NUCLEOLAR PROTEIN NO38)
2	9.61e+003	9/80 (11%)	32560.3 / 4.62	MOUSE	NUCLEOPHOSMIN (NPM) (NUCLEOLAR PHOSPHOPROTEIN B23) (NUMATRIN) (NUCLEOLAR PROTEIN NO38)
3	648	7/80 (8%)	66018.2 / 8.16	HUMAN	KERATIN, TYPE II CYTOSKELETAL 1 (CYTOKERATIN 1) (K1) (CK 1) (67 KDA CYTOKERATIN) (HAIR ALPHA PROTEIN)
4	426	9/80 (11%)	88949.1 / 8.65	MOUSE	CULLIN HOMOLOG 3 (CUL-3)
5	203	8/80 (10%)	88931.0 / 8.68	HUMAN	CULLIN HOMOLOG 3 (CUL-3)

Detailed Results

1. 10/80 matches (12%). 32575.3 Da, pI = 4.64. Acc. # P06748. HUMAN. NUCLEOPHOSMIN (NPM) (NUCLEOLAR PHOSPHOPROTEIN B23) (NUMATRIN) (NUCLEOLAR PROTEIN NO38).

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
599.3717	599.3881	-27.2328	151	155	(K)(V)
931.4699	931.4737	-4.0207	240	248	(K)(A)
1568.7848	1568.7305	34.6073	33	45	(K)(T)
1819.8784	1819.8437	19.0373	278	291	(R)(K)
1835.8704	1835.8387	17.3175	278	291	(R)MTDQEAIQDLWQWR(K)	1Met-ox
1994.0563	1994.0307	12.8432	213	230	(K)(Q)
2227.2942	2227.2161	35.0836	81	101	(K)(L)
2243.2918	2243.2110	36.0413	81	101	(K)MSVQPTVSLGGFEITPPVVLR(L)	1Met-ox
2573.2268	2573.2021	9.5983	25	45	(K)(T)
2945.5554	2945.4566	33.5386	46	73	(R)TVSLGAGAKDELHIVEAEAMNYEGSPIK(V)	1Met-ox

Click link below to search for another component.

Click individual mass to do a non-specific cleavage search.

The matched peptides cover 39% (116/294AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 12.5 Mean Error = 16.6814 ppm Data Tolerance = 40.8076 ppm Mean Missed Cleavages = 0.7

2. 9/80 matches (11%). 32560.3 Da, pI = 4.62. Acc. # Q61937. MOUSE. NUCLEOPHOSMIN (NPM) (NUCLEOLAR PHOSPHOPROTEIN B23) (NUMATRIN) (NUCLEOLAR PROTEIN NO38).

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
599.3717 599.3881 -27.2328 151 155 (K)(V)
931.4699 931.4737 -4.0207 238 246 (K)(A)
1568.7848 1568.7305 34.6073 33 45 (K)(T)
1819.8784 1819.8437 19.0373 276 289 (R)(K)
1835.8704 1835.8387 17.3175 276 289 (R)MTDQEAIQDLWQWR(K) 1Met-ox
2227.2942 2227.2161 35.0836 81 101 (K)(L)
2243.2918 2243.2110 36.0413 81 101 (K)MSVQPTVSLGGFEITPPVVLR(L) 1Met-ox
2573.2268 2573.2021 9.5983 25 45 (K)(T)
2945.5554 2945.4566 33.5386 46 73 (R)TVSLGAGAKDELHIVEAEAMNYEGSPIK(V) 1Met-ox

Click link below to search for another component.

Click individual mass to do a non-specific cleavage search.

The matched peptides cover 33% (98/292AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 11.3 Mean Error = 17.1078 ppm Data Tolerance = 43.1884 ppm Mean Missed Cleavages = 0.4

3. 7/80 matches (8%). 66018.2 Da, pI = 8.16. Acc. # P04264. HUMAN. KERATIN, TYPE II CYTOSKELETAL 1 (CYTOKERATIN 1) (K1) (CK 1) (67 KDA CYTOKERATIN) (HAIR ALPHA PROTEIN).

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
515.3226 515.3306 -15.4312 404 407 (R)(L)
973.5803 973.5318 49.7983 396 403 (K)(V)
1179.6398 1179.6010 32.8722 377 386 (K)(H)
1383.7379 1383.6909 34.0086 186 197 (K)(V)
1475.8136 1475.7858 18.8479 200 211 (R)(W)
1475.8136 1475.7494 43.5040 212 223 (K)(T)
1487.7728 1487.8043 -21.1168 240 251 (R)(L)
1994.0563 1993.9772 39.6704 224 239 (R)(R)

Click link below to search for another component.

Click individual mass to do a non-specific cleavage search.

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)
515.3226	515.3306	-15.4312	404	407	(R)(L)
973.5803	973.5318	49.7983	396	403	(K)(V)
1179.6398	1179.6010	32.8722	377	386	(K)(H)
1383.7379	1383.6909	34.0086	186	197	(K)(V)
1475.8136	1475.7858	18.8479	200	211	(R)(W)
1475.8136	1475.7494	43.5040	212	223	(K)(T)
1487.7728	1487.8043	-21.1168	240	251	(R)(L)
1994.0563	1993.9772	39.6704	224	239	(R)(R)

The matched peptides cover 13% (86/644AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 8.8 Mean Error = 22.7692 ppm Data Tolerance = 53.8526 ppm Mean Missed Cleavages = 0.4

4. 9/80 matches (11%). 88949.1 Da, pI = 8.65. Acc. # Q9JLV5. MOUSE. CULLIN HOMOLOG 3 (CUL-3).

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
715.3337 715.3667 -46.1258 763 768 (K)(-)
1234.7313 1234.6809 40.8462 431 439 (R)(L)
1234.7313 1234.6809 40.8462 537 546 (R)(Q)
1487.7728 1487.8110 -25.6254 337 349 (K)(S)
1819.8784 1819.8422 19.8727 570 588 (K)(K)
1838.9071 1838.9488 -22.6920 522 536 (K)(R)
1941.0310 1941.0704 -20.3141 626 642 (R)(V)
1994.0563 1994.1221 -32.9911 715 731 (R)(A)
2573.2268 2573.3112 -32.8208 650 671 (K)(V)
2945.5554 2945.6763 -41.0309 717 742 (K)(K)

Click link below to search for another component.

Click individual mass to do a non-specific cleavage search.

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)
715.3337	715.3667	-46.1258	763	768	(K)(-)
1234.7313	1234.6809	40.8462	431	439	(R)(L)
1234.7313	1234.6809	40.8462	537	546	(R)(Q)
1487.7728	1487.8110	-25.6254	337	349	(K)(S)
1819.8784	1819.8422	19.8727	570	588	(K)(K)
1838.9071	1838.9488	-22.6920	522	536	(K)(R)
1941.0310	1941.0704	-20.3141	626	642	(R)(V)
1994.0563	1994.1221	-32.9911	715	731	(R)(A)
2573.2268	2573.3112	-32.8208	650	671	(K)(V)
2945.5554	2945.6763	-41.0309	717	742	(K)(K)

The matched peptides cover 18% (139/768AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 11.3 Mean Error = -12.0035 ppm Data Tolerance = 66.1712 ppm Mean Missed Cleavages = 1.2

5. 8/80 matches (10%). 88931.0 Da, pI = 8.68. Acc. # Q13618. HUMAN. CULLIN HOMOLOG 3 (CUL-3).

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
715.3337 715.3667 -46.1258 763 768 (K)(-)
1234.7313 1234.6809 40.8462 431 439 (R)(L)
1234.7313 1234.6809 40.8462 537 546 (R)(Q)
1487.7728 1487.8110 -25.6254 337 349 (K)(S)
1819.8784 1819.8422 19.8727 570 588 (K)(K)
1838.9071 1838.9488 -22.6920 522 536 (K)(R)
1941.0310 1941.0704 -20.3141 626 642 (R)(V)
1994.0563 1994.1221 -32.9911 715 731 (R)(A)
2945.5554 2945.6763 -41.0309 717 742 (K)(K)

Click link below to search for another component.

Click individual mass to do a non-specific cleavage search.

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)
715.3337	715.3667	-46.1258	763	768	(K)(-)
1234.7313	1234.6809	40.8462	431	439	(R)(L)
1234.7313	1234.6809	40.8462	537	546	(R)(Q)
1487.7728	1487.8110	-25.6254	337	349	(K)(S)
1819.8784	1819.8422	19.8727	570	588	(K)(K)
1838.9071	1838.9488	-22.6920	522	536	(K)(R)
1941.0310	1941.0704	-20.3141	626	642	(R)(V)
1994.0563	1994.1221	-32.9911	715	731	(R)(A)
2945.5554	2945.6763	-41.0309	717	742	(K)(K)

The matched peptides cover 15% (117/768AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 10.0 Mean Error = -9.6905 ppm Data Tolerance = 68.4485 ppm Mean Missed Cleavages = 1.1