MS-Fit Search Results

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Sample ID (comment): HSG065
Database searched: SwissProt.7.2.2001
Molecular weight search (1000 - 100000 Da) selects 93834 entries.
Full pI range: 99359 entries.
Species search ( HUMAN MOUSE ) selects 11676 entries.
Combined molecular weight, pI and species searches select 10399 entries.
Pre searches select 10399 entries.
MS-Fit search selects 730 entries (results displayed for top 5 matches).

Parameters Used in Search

Considered modifications: | Peptide N-terminal Gln to pyroGlu | Oxidation of M | Protein N-terminus Acetylated |

4	75.000 ppm	monoisotopic	Trypsin	3	acrylamide	Hydrogen (H)	Free Acid (O H)
Min. # Peptides to Match	Peptide Mass Tolerance (+/-)	Peptide Masses are	Digest Used	Max. # Missed Cleavages	Cysteines Modified by	Peptide N terminus	Peptide C terminus

Result Summary

Rank	MOWSE Score	# (%) Masses Matched	Protein MW (Da)/pI	Species	Protein Name
1	1.12e+005	10/49 (20%)	32575.3 / 4.64	HUMAN	NUCLEOPHOSMIN (NPM) (NUCLEOLAR PHOSPHOPROTEIN B23) (NUMATRIN) (NUCLEOLAR PROTEIN NO38)
2	5e+004	9/49 (18%)	32560.3 / 4.62	MOUSE	NUCLEOPHOSMIN (NPM) (NUCLEOLAR PHOSPHOPROTEIN B23) (NUMATRIN) (NUCLEOLAR PROTEIN NO38)
3	432	4/49 (8%)	19294.5 / 5.33	HUMAN	DNA-DIRECTED RNA POLYMERASE II 19 KDA POLYPEPTIDE (RPB7)
4	249	10/49 (20%)	88949.1 / 8.65	MOUSE	CULLIN HOMOLOG 3 (CUL-3)
5	239	5/49 (10%)	56173.7 / 9.23	MOUSE	CYTOCHROME P450 2A12 (CYPIIA12) (STEROID HORMONES 7-ALPHA-HYDROXYLASE) (TESTOSTERONE 7-ALPHA-HYDROXYLASE)

Detailed Results

1. 10/49 matches (20%). 32575.3 Da, pI = 4.64. Acc. # P06748. HUMAN. NUCLEOPHOSMIN (NPM) (NUCLEOLAR PHOSPHOPROTEIN B23) (NUMATRIN) (NUCLEOLAR PROTEIN NO38).

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
647.2836	647.3113	-42.7974	207	212	(K)(D)
660.2827	660.3317	-74.0997	143	150	(R)(V)
931.5298	931.4737	60.3118	240	248	(K)(A)
1819.9592	1819.8437	63.4466	278	291	(R)(K)
1835.9388	1835.8387	54.5458	278	291	(R)MTDQEAIQDLWQWR(K)	1Met-ox
2227.3689	2227.2161	68.6295	81	101	(K)(L)
2243.3725	2243.2110	72.0085	81	101	(K)MSVQPTVSLGGFEITPPVVLR(L)	1Met-ox
2573.3484	2573.2021	56.8639	25	45	(K)(T)
2929.4900	2929.4617	9.6721	46	73	(R)(V)
2945.6486	2945.4566	65.1728	46	73	(R)TVSLGAGAKDELHIVEAEAMNYEGSPIK(V)	1Met-ox

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The matched peptides cover 36% (107/294AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 20.4 Mean Error = 33.3754 ppm Data Tolerance = 103.9043 ppm Mean Missed Cleavages = 0.4

2. 9/49 matches (18%). 32560.3 Da, pI = 4.62. Acc. # Q61937. MOUSE. NUCLEOPHOSMIN (NPM) (NUCLEOLAR PHOSPHOPROTEIN B23) (NUMATRIN) (NUCLEOLAR PROTEIN NO38).

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
647.2836 647.3113 -42.7974 206 211 (K)(D)
931.5298 931.4737 60.3118 238 246 (K)(A)
1819.9592 1819.8437 63.4466 276 289 (R)(K)
1835.9388 1835.8387 54.5458 276 289 (R)MTDQEAIQDLWQWR(K) 1Met-ox
2227.3689 2227.2161 68.6295 81 101 (K)(L)
2243.3725 2243.2110 72.0085 81 101 (K)MSVQPTVSLGGFEITPPVVLR(L) 1Met-ox
2573.3484 2573.2021 56.8639 25 45 (K)(T)
2929.4900 2929.4617 9.6721 46 73 (R)(V)
2945.6486 2945.4566 65.1728 46 73 (R)TVSLGAGAKDELHIVEAEAMNYEGSPIK(V) 1Met-ox

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m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
647.2836	647.3113	-42.7974	206	211	(K)(D)
931.5298	931.4737	60.3118	238	246	(K)(A)
1819.9592	1819.8437	63.4466	276	289	(R)(K)
1835.9388	1835.8387	54.5458	276	289	(R)MTDQEAIQDLWQWR(K)	1Met-ox
2227.3689	2227.2161	68.6295	81	101	(K)(L)
2243.3725	2243.2110	72.0085	81	101	(K)MSVQPTVSLGGFEITPPVVLR(L)	1Met-ox
2573.3484	2573.2021	56.8639	25	45	(K)(T)
2929.4900	2929.4617	9.6721	46	73	(R)(V)
2945.6486	2945.4566	65.1728	46	73	(R)TVSLGAGAKDELHIVEAEAMNYEGSPIK(V)	1Met-ox

The matched peptides cover 33% (99/292AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 18.4 Mean Error = 45.3171 ppm Data Tolerance = 75.6865 ppm Mean Missed Cleavages = 0.4

3. 4/49 matches (8%). 19294.5 Da, pI = 5.33. Acc. # P52433. HUMAN. DNA-DIRECTED RNA POLYMERASE II 19 KDA POLYPEPTIDE (RPB7).

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
1819.9592 1819.8384 66.3922 130 144 (K)(L)
1835.9388 1835.8333 57.4657 130 144 (K)TMDEDIVIQQDDEIR(L) 1Met-ox
2929.4900 2929.5305 -13.7954 130 154 (K)(N)
2945.6486 2945.5254 41.8313 130 154 (K)TMDEDIVIQQDDEIRLKIVGTRVDK(N) 1Met-ox

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m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
1819.9592	1819.8384	66.3922	130	144	(K)(L)
1835.9388	1835.8333	57.4657	130	144	(K)TMDEDIVIQQDDEIR(L)	1Met-ox
2929.4900	2929.5305	-13.7954	130	154	(K)(N)
2945.6486	2945.5254	41.8313	130	154	(K)TMDEDIVIQQDDEIRLKIVGTRVDK(N)	1Met-ox

The matched peptides cover 14% (25/172AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 8.2 Mean Error = 37.9734 ppm Data Tolerance = 71.9488 ppm Mean Missed Cleavages = 1.5

4. 10/49 matches (20%). 88949.1 Da, pI = 8.65. Acc. # Q9JLV5. MOUSE. CULLIN HOMOLOG 3 (CUL-3).

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
1819.9592 1819.8422 64.2820 570 588 (K)(K)
1941.0691 1941.0704 -0.6837 626 642 (R)(V)
1963.0296 1962.9417 44.7580 290 305 (K)NGKTEDLACMYKLFSR(V) 1Met-ox
1964.9855 1965.0704 -43.1980 717 733 (K)(F)
2247.3180 2247.1708 65.4746 60 78 (R)NAYTMVLHKHGEKLYTGLR(E) 1Met-ox
2283.3236 2283.1841 61.1093 248 266 (R)(E)
2299.2628 2299.1790 36.4466 248 266 (R)VMHCLDKSTEEPIVKVVER(E) 1Met-ox
2573.3484 2573.3112 14.4428 650 671 (K)(V)
2929.4900 2929.5159 -8.8103 323 349 (R)(S)
2945.6486 2945.6763 -9.3992 717 742 (K)(K)

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m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
1819.9592	1819.8422	64.2820	570	588	(K)(K)
1941.0691	1941.0704	-0.6837	626	642	(R)(V)
1963.0296	1962.9417	44.7580	290	305	(K)NGKTEDLACMYKLFSR(V)	1Met-ox
1964.9855	1965.0704	-43.1980	717	733	(K)(F)
2247.3180	2247.1708	65.4746	60	78	(R)NAYTMVLHKHGEKLYTGLR(E)	1Met-ox
2283.3236	2283.1841	61.1093	248	266	(R)(E)
2299.2628	2299.1790	36.4466	248	266	(R)VMHCLDKSTEEPIVKVVER(E)	1Met-ox
2573.3484	2573.3112	14.4428	650	671	(K)(V)
2929.4900	2929.5159	-8.8103	323	349	(R)(S)
2945.6486	2945.6763	-9.3992	717	742	(K)(K)

The matched peptides cover 21% (165/768AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 20.4 Mean Error = 22.4422 ppm Data Tolerance = 75.0989 ppm Mean Missed Cleavages = 1.6

5. 5/49 matches (10%). 56173.7 Da, pI = 9.23. Acc. # P56593. MOUSE. CYTOCHROME P450 2A12 (CYPIIA12) (STEROID HORMONES 7-ALPHA-HYDROXYLASE) (TESTOSTERONE 7-ALPHA-HYDROXYLASE).

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
2247.3180 2247.3018 7.2063 32 51 (K)(D)
2284.3115 2284.2189 40.5434 465 484 (R)(Y)
2573.3484 2573.5084 -62.1789 28 50 (K)(K)
2929.4900 2929.7027 -72.5768 1 27 (-)(I)
2945.6486 2945.6976 -16.6341 1 27 (-)MLGSGLLLLAILAFLSVMVLVSVWQQK(I) 1Met-ox

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Click individual mass to do a non-specific cleavage search.

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
2247.3180	2247.3018	7.2063	32	51	(K)(D)
2284.3115	2284.2189	40.5434	465	484	(R)(Y)
2573.3484	2573.5084	-62.1789	28	50	(K)(K)
2929.4900	2929.7027	-72.5768	1	27	(-)(I)
2945.6486	2945.6976	-16.6341	1	27	(-)MLGSGLLLLAILAFLSVMVLVSVWQQK(I)	1Met-ox

The matched peptides cover 14% (71/492AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 10.2 Mean Error = -20.7280 ppm Data Tolerance = 94.6454 ppm Mean Missed Cleavages = 1.0