MS-Fit Search Results

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Sample ID (comment): HSG066
Database searched: SwissProt.7.2.2001
Molecular weight search (1000 - 100000 Da) selects 93834 entries.
Full pI range: 99359 entries.
Species search ( HUMAN MOUSE ) selects 11676 entries.
Combined molecular weight, pI and species searches select 10399 entries.
Pre searches select 10399 entries.
MS-Fit search selects 1261 entries (results displayed for top 5 matches).

Parameters Used in Search

Considered modifications: | Peptide N-terminal Gln to pyroGlu | Oxidation of M | Protein N-terminus Acetylated |

4	50.000 ppm	monoisotopic	Trypsin	3	acrylamide	Hydrogen (H)	Free Acid (O H)
Min. # Peptides to Match	Peptide Mass Tolerance (+/-)	Peptide Masses are	Digest Used	Max. # Missed Cleavages	Cysteines Modified by	Peptide N terminus	Peptide C terminus

Result Summary

Rank	MOWSE Score	# (%) Masses Matched	Protein MW (Da)/pI	Species	Protein Name
1	1.82e+011	30/60 (50%)	49671.3 / 4.78	MOUSE	TUBULIN BETA-5 CHAIN
2	1.15e+011	28/60 (46%)	49831.5 / 4.79	HUMAN	TUBULIN BETA-2 CHAIN
3	4.98e+010	26/60 (43%)	49759.4 / 4.75	HUMAN	TUBULIN BETA-1 CHAIN
4	6.19e+007	21/60 (35%)	50518.3 / 4.86	HUMAN	TUBULIN BETA-4 CHAIN (TUBULIN BETA-III)
5	3.48e+007	20/60 (33%)	49631.3 / 4.81	HUMAN	TUBULIN BETA-5 CHAIN

Detailed Results

1. 30/60 matches (50%). 49671.3 Da, pI = 4.78. Acc. # P05218. MOUSE. TUBULIN BETA-5 CHAIN.

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
1039.6064	1039.5940	11.8844	310	318	(R)(G)
1042.5650	1042.5355	28.2744	351	359	(K)(G)
1077.5408	1077.5329	7.3099	155	162	(K)(I)
1130.6170	1130.5958	18.7636	242	251	(R)(K)
1143.6557	1143.6349	18.2235	253	262	(K)(L)
1159.6561	1159.6298	22.7188	253	262	(K)LAVNMVPFPR(L)	1Met-ox
1229.6309	1229.5989	26.0333	381	390	(R)(R)
1245.6238	1245.5938	24.1089	381	390	(R)ISEQFTAMFR(R)	1Met-ox
1258.7241	1258.6908	26.4362	242	252	(R)(L)
1271.7676	1271.7298	29.7429	252	262	(R)(L)
1287.7565	1287.7247	24.7012	252	262	(R)KLAVNMVPFPR(L)	1Met-ox
1301.6575	1301.6378	15.1844	47	58	(R)(Y)
1385.7304	1385.7000	21.9287	380	390	(K)(R)
1385.7304	1385.7000	21.9287	381	391	(R)(K)
1401.7462	1401.6949	36.5814	380	390	(K)RISEQFTAMFR(R)	1Met-ox
1401.7462	1401.6949	36.5814	381	391	(R)ISEQFTAMFRR(K)	1Met-ox
1615.8884	1615.8365	32.1131	63	77	(R)(S)
1620.8898	1620.8361	33.1422	263	276	(R)(G)
1631.8844	1631.8314	32.4493	63	77	(R)AILVDLEPGTMDSVR(S)	1Met-ox
1636.8845	1636.8310	32.6792	263	276	(R)LHFFMPGFAPLTSR(G)	1Met-ox
1659.9380	1659.8958	25.4330	283	297	(R)(N)
1696.8799	1696.8335	27.3503	337	350	(K)(T)
1836.9902	1836.9390	27.8303	3	19	(R)(F)
1870.0401	1869.9784	32.9925	363	379	(K)(R)
1959.0750	1958.9823	47.3230	104	121	(K)(K)
2026.1354	2026.0796	27.5800	363	380	(K)(I)
2087.1683	2087.0773	43.6030	104	122	(K)(E)
2124.1846	2124.0806	48.9392	1	19	(-)(F)
2736.4519	2736.3159	49.7121	283	306	(R)ALTVPELTQQVFDAKNMMAACDPR(H)	1Met-ox
2738.4809	2738.3493	48.0364	217	241	(K)LTTPTYGDLNHLVSATMSGVTTCLR(F)	1Met-ox
2798.4687	2798.3439	44.5977	78	103	(R)(G)
3102.5851	3102.4923	29.8956	283	309	(R)ALTVPELTQQVFDAKNMMAACDPRHGR(Y)	2Met-ox

Click link below to search for another component.

Click individual mass to do a non-specific cleavage search.

The matched peptides cover 55% (247/444AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 50.0 Mean Error = 29.8150 ppm Data Tolerance = 21.3610 ppm Mean Missed Cleavages = 0.4

2. 28/60 matches (46%). 49831.5 Da, pI = 4.79. Acc. # P05217. HUMAN. TUBULIN BETA-2 CHAIN.

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
1039.6064 1039.5940 11.8844 310 318 (R)(G)
1042.5650 1042.5355 28.2744 351 359 (K)(G)
1077.5408 1077.5329 7.3099 155 162 (K)(I)
1130.6170 1130.5958 18.7636 242 251 (R)(K)
1143.6557 1143.6349 18.2235 253 262 (K)(L)
1159.6561 1159.6298 22.7188 253 262 (K)LAVNMVPFPR(L) 1Met-ox
1229.6309 1229.5989 26.0333 381 390 (R)(R)
1245.6238 1245.5938 24.1089 381 390 (R)ISEQFTAMFR(R) 1Met-ox
1258.7241 1258.6908 26.4362 242 252 (R)(L)
1271.7676 1271.7298 29.7429 252 262 (R)(L)
1287.7565 1287.7247 24.7012 252 262 (R)KLAVNMVPFPR(L) 1Met-ox
1328.6806 1328.6487 24.0738 47 58 (R)(Y)
1385.7304 1385.7000 21.9287 380 390 (K)(R)
1385.7304 1385.7000 21.9287 381 391 (R)(K)
1401.7462 1401.6949 36.5814 380 390 (K)RISEQFTAMFR(R) 1Met-ox
1401.7462 1401.6949 36.5814 381 391 (R)ISEQFTAMFRR(K) 1Met-ox
1601.8861 1601.8209 40.7190 63 77 (R)(S)
1620.8898 1620.8361 33.1422 263 276 (R)(G)
1636.8845 1636.8310 32.6792 263 276 (R)LHFFMPGFAPLTSR(G) 1Met-ox
1691.9127 1691.8678 26.4958 283 297 (R)(N)
1696.8799 1696.8335 27.3503 337 350 (K)(T)
1707.9031 1707.8627 23.6417 283 297 (R)ALTVPELTQQMFDAK(N) 1Met-ox
1836.9902 1836.9390 27.8303 3 19 (R)(F)
1858.0296 1857.9421 47.0912 363 379 (K)(R)
1959.0750 1958.9823 47.3230 104 121 (K)(K)
2087.1683 2087.0773 43.6030 104 122 (K)(E)
2124.1846 2124.0806 48.9392 1 19 (-)(F)
2738.4809 2738.3493 48.0364 217 241 (K)LTTPTYGDLNHLVSATMSGVTTCLR(F) 1Met-ox
2798.4687 2798.3439 44.5977 78 103 (R)(G)
3102.5851 3102.4746 35.6197 283 309 (R)(Y)

Click link below to search for another component.

Click individual mass to do a non-specific cleavage search.

The matched peptides cover 55% (247/445AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 46.7 Mean Error = 30.2120 ppm Data Tolerance = 21.6818 ppm Mean Missed Cleavages = 0.4

3. 26/60 matches (43%). 49759.4 Da, pI = 4.75. Acc. # P07437. HUMAN. TUBULIN BETA-1 CHAIN.

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
1039.6064 1039.5940 11.8844 310 318 (R)(G)
1042.5650 1042.5355 28.2744 351 359 (K)(G)
1077.5408 1077.5329 7.3099 155 162 (K)(I)
1130.6170 1130.5958 18.7636 242 251 (R)(K)
1143.6557 1143.6349 18.2235 253 262 (K)(L)
1159.6561 1159.6298 22.7188 253 262 (K)LAVNMVPFPR(L) 1Met-ox
1229.6309 1229.5989 26.0333 381 390 (R)(R)
1245.6238 1245.5938 24.1089 381 390 (R)ISEQFTAMFR(R) 1Met-ox
1258.7241 1258.6908 26.4362 242 252 (R)(L)
1271.7676 1271.7298 29.7429 252 262 (R)(L)
1287.7565 1287.7247 24.7012 252 262 (R)KLAVNMVPFPR(L) 1Met-ox
1301.6575 1301.6378 15.1844 47 58 (R)(Y)
1385.7304 1385.7000 21.9287 380 390 (K)(R)
1385.7304 1385.7000 21.9287 381 391 (R)(K)
1401.7462 1401.6949 36.5814 380 390 (K)RISEQFTAMFR(R) 1Met-ox
1401.7462 1401.6949 36.5814 381 391 (R)ISEQFTAMFRR(K) 1Met-ox
1615.8884 1615.8365 32.1131 63 77 (R)(S)
1620.8898 1620.8361 33.1422 263 276 (R)(G)
1631.8844 1631.8314 32.4493 63 77 (R)AILVDLEPGTMDSVR(S) 1Met-ox
1636.8845 1636.8310 32.6792 263 276 (R)LHFFMPGFAPLTSR(G) 1Met-ox
1696.8799 1696.8335 27.3503 337 350 (K)(T)
1836.9902 1836.9390 27.8303 3 19 (R)(F)
1870.0401 1869.9784 32.9925 363 379 (K)(R)
1959.0750 1958.9823 47.3230 104 121 (K)(K)
2026.1354 2026.0796 27.5800 363 380 (K)(I)
2087.1683 2087.0773 43.6030 104 122 (K)(E)
2124.1846 2124.0806 48.9392 1 19 (-)(F)
2798.4687 2798.3439 44.5977 78 103 (R)(G)

Click link below to search for another component.

Click individual mass to do a non-specific cleavage search.

The matched peptides cover 43% (195/444AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 43.3 Mean Error = 28.6072 ppm Data Tolerance = 20.1781 ppm Mean Missed Cleavages = 0.4

4. 21/60 matches (35%). 50518.3 Da, pI = 4.86. Acc. # Q13509. HUMAN. TUBULIN BETA-4 CHAIN (TUBULIN BETA-III).

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
1130.6170 1130.5958 18.7636 242 251 (R)(K)
1143.6557 1143.6349 18.2235 253 262 (K)(L)
1159.6561 1159.6298 22.7188 253 262 (K)LAVNMVPFPR(L) 1Met-ox
1229.6309 1229.5989 26.0333 381 390 (R)(R)
1245.6238 1245.5938 24.1089 381 390 (R)ISEQFTAMFR(R) 1Met-ox
1258.7241 1258.6908 26.4362 242 252 (R)(L)
1271.7676 1271.7298 29.7429 252 262 (R)(L)
1287.7565 1287.7247 24.7012 252 262 (R)KLAVNMVPFPR(L) 1Met-ox
1385.7304 1385.7000 21.9287 380 390 (K)(R)
1385.7304 1385.7000 21.9287 381 391 (R)(K)
1401.7462 1401.6949 36.5814 380 390 (K)RISEQFTAMFR(R) 1Met-ox
1401.7462 1401.6949 36.5814 381 391 (R)ISEQFTAMFRR(K) 1Met-ox
1615.8884 1615.8365 32.1131 63 77 (R)(S)
1631.8844 1631.8314 32.4493 63 77 (R)AILVDLEPGTMDSVR(S) 1Met-ox
1691.9127 1691.8678 26.4958 283 297 (R)(N)
1696.8799 1696.8335 27.3503 337 350 (K)(V)
1707.9031 1707.8627 23.6417 283 297 (R)ALTVPELTQQMFDAK(N) 1Met-ox
1836.9902 1836.9390 27.8303 3 19 (R)(F)
1959.0750 1958.9823 47.3230 104 121 (K)(K)
2087.1683 2087.0773 43.6030 104 122 (K)(E)
2124.1846 2124.0806 48.9392 1 19 (-)(F)
2814.4767 2814.5216 -15.9796 253 276 (K)(G)
3102.5851 3102.4746 35.6197 283 309 (R)(Y)

Click link below to search for another component.

Click individual mass to do a non-specific cleavage search.

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
1130.6170	1130.5958	18.7636	242	251	(R)(K)
1143.6557	1143.6349	18.2235	253	262	(K)(L)
1159.6561	1159.6298	22.7188	253	262	(K)LAVNMVPFPR(L)	1Met-ox
1229.6309	1229.5989	26.0333	381	390	(R)(R)
1245.6238	1245.5938	24.1089	381	390	(R)ISEQFTAMFR(R)	1Met-ox
1258.7241	1258.6908	26.4362	242	252	(R)(L)
1271.7676	1271.7298	29.7429	252	262	(R)(L)
1287.7565	1287.7247	24.7012	252	262	(R)KLAVNMVPFPR(L)	1Met-ox
1385.7304	1385.7000	21.9287	380	390	(K)(R)
1385.7304	1385.7000	21.9287	381	391	(R)(K)
1401.7462	1401.6949	36.5814	380	390	(K)RISEQFTAMFR(R)	1Met-ox
1401.7462	1401.6949	36.5814	381	391	(R)ISEQFTAMFRR(K)	1Met-ox
1615.8884	1615.8365	32.1131	63	77	(R)(S)
1631.8844	1631.8314	32.4493	63	77	(R)AILVDLEPGTMDSVR(S)	1Met-ox
1691.9127	1691.8678	26.4958	283	297	(R)(N)
1696.8799	1696.8335	27.3503	337	350	(K)(V)
1707.9031	1707.8627	23.6417	283	297	(R)ALTVPELTQQMFDAK(N)	1Met-ox
1836.9902	1836.9390	27.8303	3	19	(R)(F)
1959.0750	1958.9823	47.3230	104	121	(K)(K)
2087.1683	2087.0773	43.6030	104	122	(K)(E)
2124.1846	2124.0806	48.9392	1	19	(-)(F)
2814.4767	2814.5216	-15.9796	253	276	(K)(G)
3102.5851	3102.4746	35.6197	283	309	(R)(Y)

The matched peptides cover 31% (141/450AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 35.0 Mean Error = 27.7015 ppm Data Tolerance = 25.4740 ppm Mean Missed Cleavages = 0.6

5. 20/60 matches (33%). 49631.3 Da, pI = 4.81. Acc. # P04350. HUMAN. TUBULIN BETA-5 CHAIN.

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
1039.6064 1039.5940 11.8844 310 318 (R)(G)
1042.5650 1042.5355 28.2744 351 359 (K)(G)
1130.6170 1130.5958 18.7636 242 251 (R)(K)
1143.6557 1143.6349 18.2235 253 262 (K)(L)
1159.6561 1159.6298 22.7188 253 262 (K)LAVNMVPFPR(L) 1Met-ox
1229.6309 1229.5989 26.0333 381 390 (R)(R)
1245.6238 1245.5938 24.1089 381 390 (R)ISEQFTAMFR(R) 1Met-ox
1258.7241 1258.6908 26.4362 242 252 (R)(L)
1271.7676 1271.7298 29.7429 252 262 (R)(L)
1287.7565 1287.7247 24.7012 252 262 (R)KLAVNMVPFPR(L) 1Met-ox
1385.7304 1385.7000 21.9287 380 390 (K)(R)
1385.7304 1385.7000 21.9287 381 391 (R)(K)
1401.7462 1401.6949 36.5814 380 390 (K)RISEQFTAMFR(R) 1Met-ox
1401.7462 1401.6949 36.5814 381 391 (R)ISEQFTAMFRR(K) 1Met-ox
1601.8861 1601.8209 40.7190 63 77 (R)(S)
1696.8799 1696.8335 27.3503 337 350 (K)(T)
1836.9902 1836.9390 27.8303 3 19 (R)(F)
1870.0401 1869.9784 32.9925 363 379 (K)(R)
2026.1354 2026.0796 27.5800 363 380 (K)(I)
2124.1846 2124.0806 48.9392 1 19 (-)(F)
2738.4809 2738.3493 48.0364 217 241 (K)LTTPTYGDLNHLVSATMSGVTTCLR(F) 1Met-ox
2798.4687 2798.3439 44.5977 78 103 (R)(G)

Click link below to search for another component.

Click individual mass to do a non-specific cleavage search.

The matched peptides cover 37% (167/444AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 33.3 Mean Error = 29.3615 ppm Data Tolerance = 19.4539 ppm Mean Missed Cleavages = 0.4