MS-Fit Search Results

Press stop on your browser if you wish to abort this MS-Fit search prematurely.
Sample ID (comment): HSG067
Database searched: SwissProt.7.2.2001
Molecular weight search (1000 - 100000 Da) selects 93834 entries.
Full pI range: 99359 entries.
Species search ( HUMAN MOUSE ) selects 11676 entries.
Combined molecular weight, pI and species searches select 10399 entries.
Pre searches select 10399 entries.
MS-Fit search selects 387 entries (results displayed for top 5 matches).

Parameters Used in Search

Considered modifications: | Peptide N-terminal Gln to pyroGlu | Oxidation of M | Protein N-terminus Acetylated |

4	25.000 ppm	monoisotopic	Trypsin	3	acrylamide	Hydrogen (H)	Free Acid (O H)
Min. # Peptides to Match	Peptide Mass Tolerance (+/-)	Peptide Masses are	Digest Used	Max. # Missed Cleavages	Cysteines Modified by	Peptide N terminus	Peptide C terminus

Result Summary

Rank	MOWSE Score	# (%) Masses Matched	Protein MW (Da)/pI	Species	Protein Name
1	7.27e+014	34/67 (50%)	49671.3 / 4.78	MOUSE	TUBULIN BETA-5 CHAIN
2	4.95e+013	33/67 (49%)	49831.5 / 4.79	HUMAN	TUBULIN BETA-2 CHAIN
3	6.67e+012	32/67 (47%)	49759.4 / 4.75	HUMAN	TUBULIN BETA-1 CHAIN
4	1.39e+011	28/67 (41%)	49631.3 / 4.81	HUMAN	TUBULIN BETA-5 CHAIN
5	3.45e+008	23/67 (34%)	50518.3 / 4.86	HUMAN	TUBULIN BETA-4 CHAIN (TUBULIN BETA-III)

Detailed Results

1. 34/67 matches (50%). 49671.3 Da, pI = 4.78. Acc. # P05218. MOUSE. TUBULIN BETA-5 CHAIN.

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
1039.5723	1039.5940	-20.8649	310	318	(R)(G)
1042.5342	1042.5355	-1.2756	351	359	(K)(G)
1077.5120	1077.5329	-19.3622	155	162	(K)(I)
1079.4436	1079.4436	0.0017	298	306	(K)(H)
1130.5804	1130.5958	-13.6061	242	251	(R)(K)
1143.6217	1143.6349	-11.4818	253	262	(K)(L)
1159.6088	1159.6298	-18.1167	253	262	(K)LAVNMVPFPR(L)	1Met-ox
1229.5904	1229.5989	-6.8880	381	390	(R)(R)
1245.5807	1245.5938	-10.5324	381	390	(R)ISEQFTAMFR(R)	1Met-ox
1258.6875	1258.6908	-2.6448	242	252	(R)(L)
1271.7249	1271.7298	-3.8436	252	262	(R)(L)
1287.7081	1287.7247	-12.9155	252	262	(R)KLAVNMVPFPR(L)	1Met-ox
1301.6322	1301.6378	-4.2864	47	58	(R)(Y)
1319.6794	1319.7033	-18.1143	163	174	(R)(V)
1335.6656	1335.6982	-24.4278	163	174	(R)IMNTFSVVPSPK(V)	1Met-ox
1385.6978	1385.7000	-1.5815	380	390	(K)(R)
1385.6978	1385.7000	-1.5815	381	391	(R)(K)
1401.6844	1401.6949	-7.4781	380	390	(K)RISEQFTAMFR(R)	1Met-ox
1401.6844	1401.6949	-7.4781	381	391	(R)ISEQFTAMFRR(K)	1Met-ox
1615.8347	1615.8365	-1.1644	63	77	(R)(S)
1620.8199	1620.8361	-9.9751	263	276	(R)(G)
1631.8258	1631.8314	-3.4318	63	77	(R)AILVDLEPGTMDSVR(S)	1Met-ox
1636.8357	1636.8310	2.8814	263	276	(R)LHFFMPGFAPLTSR(G)	1Met-ox
1659.8701	1659.8958	-15.4455	283	297	(R)(N)
1696.8161	1696.8335	-10.2373	337	350	(K)(T)
1836.9109	1836.9390	-15.3236	3	19	(R)(F)
1869.9871	1869.9784	4.6243	363	379	(K)(R)
1885.9464	1885.9734	-14.2777	363	379	(K)MAVTFIGNSTAIQELFK(R)	1Met-ox
1958.9899	1958.9823	3.8657	104	121	(K)(K)
2087.0986	2087.0773	10.2190	104	122	(K)(E)
2124.1123	2124.0806	14.9019	1	19	(-)(F)
2140.0889	2140.0756	6.2424	1	19	(-)MREIVHIQAGQCGNQIGAK(F)	1Met-ox
2722.4121	2722.3544	21.1964	217	241	(K)(F)
2738.3568	2738.3493	2.7310	217	241	(K)LTTPTYGDLNHLVSATMSGVTTCLR(F)	1Met-ox
2798.3741	2798.3439	10.7998	78	103	(R)(G)
3102.4654	3102.4081	18.4675	20	46	(K)(I)
3102.4654	3102.4923	-8.6792	283	309	(R)ALTVPELTQQVFDAKNMMAACDPRHGR(Y)	2Met-ox

Click link below to search for another component.

Click individual mass to do a non-specific cleavage search.

The matched peptides cover 64% (286/444AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 50.7 Mean Error = -4.5698 ppm Data Tolerance = 22.0754 ppm Mean Missed Cleavages = 0.4

2. 33/67 matches (49%). 49831.5 Da, pI = 4.79. Acc. # P05217. HUMAN. TUBULIN BETA-2 CHAIN.

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
1039.5723 1039.5940 -20.8649 310 318 (R)(G)
1042.5342 1042.5355 -1.2756 351 359 (K)(G)
1077.5120 1077.5329 -19.3622 155 162 (K)(I)
1079.4436 1079.4436 0.0017 298 306 (K)(H)
1130.5804 1130.5958 -13.6061 242 251 (R)(K)
1143.6217 1143.6349 -11.4818 253 262 (K)(L)
1159.6088 1159.6298 -18.1167 253 262 (K)LAVNMVPFPR(L) 1Met-ox
1229.5904 1229.5989 -6.8880 381 390 (R)(R)
1245.5807 1245.5938 -10.5324 381 390 (R)ISEQFTAMFR(R) 1Met-ox
1258.6875 1258.6908 -2.6448 242 252 (R)(L)
1271.7249 1271.7298 -3.8436 252 262 (R)(L)
1287.7081 1287.7247 -12.9155 252 262 (R)KLAVNMVPFPR(L) 1Met-ox
1319.6794 1319.7033 -18.1143 163 174 (R)(V)
1328.6531 1328.6487 3.3550 47 58 (R)(Y)
1335.6656 1335.6982 -24.4278 163 174 (R)IMNTFSVVPSPK(V) 1Met-ox
1385.6978 1385.7000 -1.5815 380 390 (K)(R)
1385.6978 1385.7000 -1.5815 381 391 (R)(K)
1401.6844 1401.6949 -7.4781 380 390 (K)RISEQFTAMFR(R) 1Met-ox
1401.6844 1401.6949 -7.4781 381 391 (R)ISEQFTAMFRR(K) 1Met-ox
1601.8281 1601.8209 4.5321 63 77 (R)(S)
1620.8199 1620.8361 -9.9751 263 276 (R)(G)
1636.8357 1636.8310 2.8814 263 276 (R)LHFFMPGFAPLTSR(G) 1Met-ox
1691.8589 1691.8678 -5.3052 283 297 (R)(N)
1696.8161 1696.8335 -10.2373 337 350 (K)(T)
1836.9109 1836.9390 -15.3236 3 19 (R)(F)
1857.8990 1857.9421 -23.1779 363 379 (K)(R)
1958.9899 1958.9823 3.8657 104 121 (K)(K)
2087.0986 2087.0773 10.2190 104 122 (K)(E)
2124.1123 2124.0806 14.9019 1 19 (-)(F)
2140.0889 2140.0756 6.2424 1 19 (-)MREIVHLQAGQCGNQIGAK(F) 1Met-ox
2722.4121 2722.3544 21.1964 217 241 (K)(F)
2738.3568 2738.3493 2.7310 217 241 (K)LTTPTYGDLNHLVSATMSGVTTCLR(F) 1Met-ox
2798.3741 2798.3439 10.7998 78 103 (R)(G)
3102.4654 3102.4746 -2.9553 283 309 (R)(Y)
3116.4391 3116.4237 4.9337 20 46 (K)(I)

Click link below to search for another component.

Click individual mass to do a non-specific cleavage search.

The matched peptides cover 64% (286/445AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 49.3 Mean Error = -4.6716 ppm Data Tolerance = 21.9981 ppm Mean Missed Cleavages = 0.4

3. 32/67 matches (47%). 49759.4 Da, pI = 4.75. Acc. # P07437. HUMAN. TUBULIN BETA-1 CHAIN.

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
1039.5723 1039.5940 -20.8649 310 318 (R)(G)
1042.5342 1042.5355 -1.2756 351 359 (K)(G)
1077.5120 1077.5329 -19.3622 155 162 (K)(I)
1079.4436 1079.4436 0.0017 298 306 (K)(H)
1130.5804 1130.5958 -13.6061 242 251 (R)(K)
1143.6217 1143.6349 -11.4818 253 262 (K)(L)
1159.6088 1159.6298 -18.1167 253 262 (K)LAVNMVPFPR(L) 1Met-ox
1229.5904 1229.5989 -6.8880 381 390 (R)(R)
1245.5807 1245.5938 -10.5324 381 390 (R)ISEQFTAMFR(R) 1Met-ox
1258.6875 1258.6908 -2.6448 242 252 (R)(L)
1271.7249 1271.7298 -3.8436 252 262 (R)(L)
1287.7081 1287.7247 -12.9155 252 262 (R)KLAVNMVPFPR(L) 1Met-ox
1301.6322 1301.6378 -4.2864 47 58 (R)(Y)
1319.6794 1319.7033 -18.1143 163 174 (R)(V)
1335.6656 1335.6982 -24.4278 163 174 (R)IMNTFSVVPSPK(V) 1Met-ox
1385.6978 1385.7000 -1.5815 380 390 (K)(R)
1385.6978 1385.7000 -1.5815 381 391 (R)(K)
1401.6844 1401.6949 -7.4781 380 390 (K)RISEQFTAMFR(R) 1Met-ox
1401.6844 1401.6949 -7.4781 381 391 (R)ISEQFTAMFRR(K) 1Met-ox
1615.8347 1615.8365 -1.1644 63 77 (R)(S)
1620.8199 1620.8361 -9.9751 263 276 (R)(G)
1631.8258 1631.8314 -3.4318 63 77 (R)AILVDLEPGTMDSVR(S) 1Met-ox
1636.8357 1636.8310 2.8814 263 276 (R)LHFFMPGFAPLTSR(G) 1Met-ox
1696.8161 1696.8335 -10.2373 337 350 (K)(T)
1836.9109 1836.9390 -15.3236 3 19 (R)(F)
1869.9871 1869.9784 4.6243 363 379 (K)(R)
1885.9464 1885.9734 -14.2777 363 379 (K)MAVTFIGNSTAIQELFK(R) 1Met-ox
1958.9899 1958.9823 3.8657 104 121 (K)(K)
2087.0986 2087.0773 10.2190 104 122 (K)(E)
2124.1123 2124.0806 14.9019 1 19 (-)(F)
2140.0889 2140.0756 6.2424 1 19 (-)MREIVHIQAGQCGNQIGAK(F) 1Met-ox
2738.3568 2738.2952 22.5040 283 306 (R)ALTVPDLTQQVFDAKNMMAACDPR(H) 2Met-ox
2798.3741 2798.3439 10.7998 78 103 (R)(G)
3102.4654 3102.4081 18.4675 20 46 (K)(I)

Click link below to search for another component.

Click individual mass to do a non-specific cleavage search.

The matched peptides cover 58% (258/444AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 47.8 Mean Error = -4.3053 ppm Data Tolerance = 22.7347 ppm Mean Missed Cleavages = 0.4

4. 28/67 matches (41%). 49631.3 Da, pI = 4.81. Acc. # P04350. HUMAN. TUBULIN BETA-5 CHAIN.

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
1039.5723 1039.5940 -20.8649 310 318 (R)(G)
1042.5342 1042.5355 -1.2756 351 359 (K)(G)
1079.4436 1079.4436 0.0017 298 306 (K)(H)
1095.4826 1095.4893 -6.1443 155 162 (K)MREEFPDR(I) 1Met-ox
1130.5804 1130.5958 -13.6061 242 251 (R)(K)
1143.6217 1143.6349 -11.4818 253 262 (K)(L)
1159.6088 1159.6298 -18.1167 253 262 (K)LAVNMVPFPR(L) 1Met-ox
1229.5904 1229.5989 -6.8880 381 390 (R)(R)
1245.5807 1245.5938 -10.5324 381 390 (R)ISEQFTAMFR(R) 1Met-ox
1258.6875 1258.6908 -2.6448 242 252 (R)(L)
1271.7249 1271.7298 -3.8436 252 262 (R)(L)
1287.7081 1287.7247 -12.9155 252 262 (R)KLAVNMVPFPR(L) 1Met-ox
1319.6794 1319.7033 -18.1143 163 174 (R)(V)
1335.6656 1335.6982 -24.4278 163 174 (R)IMNTFSVVPSPK(V) 1Met-ox
1385.6978 1385.7000 -1.5815 380 390 (K)(R)
1385.6978 1385.7000 -1.5815 381 391 (R)(K)
1401.6844 1401.6949 -7.4781 380 390 (K)RISEQFTAMFR(R) 1Met-ox
1401.6844 1401.6949 -7.4781 381 391 (R)ISEQFTAMFRR(K) 1Met-ox
1601.8281 1601.8209 4.5321 63 77 (R)(S)
1696.8161 1696.8335 -10.2373 337 350 (K)(T)
1836.9109 1836.9390 -15.3236 3 19 (R)(F)
1869.9871 1869.9784 4.6243 363 379 (K)(R)
1885.9464 1885.9734 -14.2777 363 379 (K)MAVTFIGNSTAIQELFK(R) 1Met-ox
2124.1123 2124.0806 14.9019 1 19 (-)(F)
2140.0889 2140.0756 6.2424 1 19 (-)MREIVHLQAGQCGNQIGAK(F) 1Met-ox
2722.4121 2722.3544 21.1964 217 241 (K)(F)
2738.3568 2738.3493 2.7310 217 241 (K)LTTPTYGDLNHLVSATMSGVTTCLR(F) 1Met-ox
2798.3741 2798.3439 10.7998 78 103 (R)(G)
3104.4195 3104.4538 -11.0600 283 309 (R)GLTVPELTQQMFDAKNMMAACDPRHGR(Y) 1Met-ox
3116.4391 3116.4237 4.9337 20 46 (K)(I)

Click link below to search for another component.

Click individual mass to do a non-specific cleavage search.

The matched peptides cover 54% (241/444AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 41.8 Mean Error = -4.9970 ppm Data Tolerance = 21.4184 ppm Mean Missed Cleavages = 0.4

5. 23/67 matches (34%). 50518.3 Da, pI = 4.86. Acc. # Q13509. HUMAN. TUBULIN BETA-4 CHAIN (TUBULIN BETA-III).

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
1079.4436 1079.4436 0.0017 298 306 (K)(H)
1130.5804 1130.5958 -13.6061 242 251 (R)(K)
1143.6217 1143.6349 -11.4818 253 262 (K)(L)
1159.6088 1159.6298 -18.1167 253 262 (K)LAVNMVPFPR(L) 1Met-ox
1229.5904 1229.5989 -6.8880 381 390 (R)(R)
1245.5807 1245.5938 -10.5324 381 390 (R)ISEQFTAMFR(R) 1Met-ox
1258.6875 1258.6908 -2.6448 242 252 (R)(L)
1271.7249 1271.7298 -3.8436 252 262 (R)(L)
1287.7081 1287.7247 -12.9155 252 262 (R)KLAVNMVPFPR(L) 1Met-ox
1319.6794 1319.7033 -18.1143 163 174 (R)(V)
1335.6656 1335.6982 -24.4278 163 174 (R)IMNTFSVVPSPK(V) 1Met-ox
1385.6978 1385.7000 -1.5815 380 390 (K)(R)
1385.6978 1385.7000 -1.5815 381 391 (R)(K)
1401.6844 1401.6949 -7.4781 380 390 (K)RISEQFTAMFR(R) 1Met-ox
1401.6844 1401.6949 -7.4781 381 391 (R)ISEQFTAMFRR(K) 1Met-ox
1615.8347 1615.8365 -1.1644 63 77 (R)(S)
1631.8258 1631.8314 -3.4318 63 77 (R)AILVDLEPGTMDSVR(S) 1Met-ox
1691.8589 1691.8678 -5.3052 283 297 (R)(N)
1696.8161 1696.8335 -10.2373 337 350 (K)(V)
1836.9109 1836.9390 -15.3236 3 19 (R)(F)
1958.9899 1958.9823 3.8657 104 121 (K)(K)
2087.0986 2087.0773 10.2190 104 122 (K)(E)
2124.1123 2124.0806 14.9019 1 19 (-)(F)
2140.0889 2140.0756 6.2424 1 19 (-)MREIVHIQAGQCGNQIGAK(F) 1Met-ox
3102.4654 3102.4746 -2.9553 283 309 (R)(Y)

Click link below to search for another component.

Click individual mass to do a non-specific cleavage search.

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
1079.4436	1079.4436	0.0017	298	306	(K)(H)
1130.5804	1130.5958	-13.6061	242	251	(R)(K)
1143.6217	1143.6349	-11.4818	253	262	(K)(L)
1159.6088	1159.6298	-18.1167	253	262	(K)LAVNMVPFPR(L)	1Met-ox
1229.5904	1229.5989	-6.8880	381	390	(R)(R)
1245.5807	1245.5938	-10.5324	381	390	(R)ISEQFTAMFR(R)	1Met-ox
1258.6875	1258.6908	-2.6448	242	252	(R)(L)
1271.7249	1271.7298	-3.8436	252	262	(R)(L)
1287.7081	1287.7247	-12.9155	252	262	(R)KLAVNMVPFPR(L)	1Met-ox
1319.6794	1319.7033	-18.1143	163	174	(R)(V)
1335.6656	1335.6982	-24.4278	163	174	(R)IMNTFSVVPSPK(V)	1Met-ox
1385.6978	1385.7000	-1.5815	380	390	(K)(R)
1385.6978	1385.7000	-1.5815	381	391	(R)(K)
1401.6844	1401.6949	-7.4781	380	390	(K)RISEQFTAMFR(R)	1Met-ox
1401.6844	1401.6949	-7.4781	381	391	(R)ISEQFTAMFRR(K)	1Met-ox
1615.8347	1615.8365	-1.1644	63	77	(R)(S)
1631.8258	1631.8314	-3.4318	63	77	(R)AILVDLEPGTMDSVR(S)	1Met-ox
1691.8589	1691.8678	-5.3052	283	297	(R)(N)
1696.8161	1696.8335	-10.2373	337	350	(K)(V)
1836.9109	1836.9390	-15.3236	3	19	(R)(F)
1958.9899	1958.9823	3.8657	104	121	(K)(K)
2087.0986	2087.0773	10.2190	104	122	(K)(E)
2124.1123	2124.0806	14.9019	1	19	(-)(F)
2140.0889	2140.0756	6.2424	1	19	(-)MREIVHIQAGQCGNQIGAK(F)	1Met-ox
3102.4654	3102.4746	-2.9553	283	309	(R)(Y)

The matched peptides cover 30% (139/450AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 34.3 Mean Error = -5.7551 ppm Data Tolerance = 18.1467 ppm Mean Missed Cleavages = 0.5