MS-Fit Search Results

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Sample ID (comment): HSG068
Database searched: SwissProt.7.2.2001
Molecular weight search (1000 - 100000 Da) selects 93834 entries.
Full pI range: 99359 entries.
Species search ( HUMAN MOUSE ) selects 11676 entries.
Combined molecular weight, pI and species searches select 10399 entries.
Pre searches select 10399 entries.
MS-Fit search selects 346 entries (results displayed for top 5 matches).

Parameters Used in Search

Considered modifications: | Peptide N-terminal Gln to pyroGlu | Oxidation of M | Protein N-terminus Acetylated |

4	30.000 ppm	monoisotopic	Trypsin	3	acrylamide	Hydrogen (H)	Free Acid (O H)
Min. # Peptides to Match	Peptide Mass Tolerance (+/-)	Peptide Masses are	Digest Used	Max. # Missed Cleavages	Cysteines Modified by	Peptide N terminus	Peptide C terminus

Result Summary

Rank	MOWSE Score	# (%) Masses Matched	Protein MW (Da)/pI	Species	Protein Name
1	1.37e+005	11/67 (16%)	47790.5 / 4.89	MOUSE	HISTONE ACETYLTRANSFERASE TYPE B SUBUNIT 2 (RETINOBLASTOMA BINDING PROTEIN P46) (RETINOBLASTOMA-BINDING PROTEIN 7)
2	1.37e+005	11/67 (16%)	47820.5 / 4.89	HUMAN	HISTONE ACETYLTRANSFERASE TYPE B SUBUNIT 2 (RETINOBLASTOMA BINDING PROTEIN P46) (RETINOBLASTOMA-BINDING PROTEIN 7)
3	1.06e+003	11/67 (16%)	53674.7 / 5.52	HUMAN	KERATIN, TYPE II CYTOSKELETAL 8 (CYTOKERATIN 8) (K8) (CK 8)
4	320	5/67 (7%)	49229.9 / 5.89	HUMAN	HETEROGENEOUS NUCLEAR RIBONUCLEOPROTEIN H (HNRNP H)
5	236	5/67 (7%)	66018.2 / 8.16	HUMAN	KERATIN, TYPE II CYTOSKELETAL 1 (CYTOKERATIN 1) (K1) (CK 1) (67 KDA CYTOKERATIN) (HAIR ALPHA PROTEIN)

Detailed Results

1. 11/67 matches (16%). 47790.5 Da, pI = 4.89. Acc. # Q60973. MOUSE. HISTONE ACETYLTRANSFERASE TYPE B SUBUNIT 2 (RETINOBLASTOMA BINDING PROTEIN P46) (RETINOBLASTOMA-BINDING PROTEIN 7).

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
934.4706	934.4820	-12.2140	251	257	(K)(S)
950.4605	950.4770	-17.3086	251	257	(K)LMIWDTR(S)	1Met-ox
973.5368	973.5471	-10.5427	296	303	(K)(N)
1130.6104	1130.6322	-19.2542	340	348	(R)(I)
1284.5667	1284.5418	19.3905	5	14	(K)(V)
1412.7256	1412.7360	-7.3656	131	142	(R)(T)
1428.6961	1428.7309	-24.4085	131	142	(R)YMPQNPHIIATK(T)	1Met-ox
1628.7422	1628.7114	18.9373	1	14	(-)ASKEMFEDTVEER(V)	1Met-ox Acet N
1803.8270	1803.8448	-9.8644	156	171	(K)(L)
2774.4131	2774.4014	4.2019	59	82	(K)(V)
2847.4095	2847.3437	23.0927	349	375	(K)(I)

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The matched peptides cover 27% (117/425AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 16.4 Mean Error = -3.2123 ppm Data Tolerance = 33.7639 ppm Mean Missed Cleavages = 0.2

2. 11/67 matches (16%). 47820.5 Da, pI = 4.89. Acc. # Q16576. HUMAN. HISTONE ACETYLTRANSFERASE TYPE B SUBUNIT 2 (RETINOBLASTOMA BINDING PROTEIN P46) (RETINOBLASTOMA-BINDING PROTEIN 7).

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
934.4706 934.4820 -12.2140 251 257 (K)(S)
950.4605 950.4770 -17.3086 251 257 (K)LMIWDTR(S) 1Met-ox
973.5368 973.5471 -10.5427 296 303 (K)(N)
1130.6104 1130.6322 -19.2542 340 348 (R)(I)
1284.5667 1284.5418 19.3905 5 14 (K)(V)
1412.7256 1412.7360 -7.3656 131 142 (R)(T)
1428.6961 1428.7309 -24.4085 131 142 (R)YMPQNPHIIATK(T) 1Met-ox
1628.7422 1628.7114 18.9373 1 14 (-)ASKEMFEDTVEER(V) 1Met-ox Acet N
1803.8270 1803.8448 -9.8644 156 171 (K)(L)
2774.4131 2774.4014 4.2019 59 82 (K)(V)
2847.4095 2847.3437 23.0927 349 375 (K)(I)

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The matched peptides cover 27% (117/425AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 16.4 Mean Error = -3.2123 ppm Data Tolerance = 33.7639 ppm Mean Missed Cleavages = 0.2

3. 11/67 matches (16%). 53674.7 Da, pI = 5.52. Acc. # P05787. HUMAN. KERATIN, TYPE II CYTOSKELETAL 8 (CYTOKERATIN 8) (K8) (CK 8).

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
1079.5447 1079.5373 6.8149 226 233 (R)(E)
1129.5942 1129.6217 -24.3695 342 352 (R)(L)
1129.5942 1129.6217 -24.3695 353 362 (K)(A)
1169.5292 1169.5512 -18.8863 276 285 (R)(Y)
1169.5292 1169.5512 -18.8863 373 381 (R)EYQELMNVK(L) 1Met-ox
1277.7115 1277.7105 0.7558 382 392 (K)(K)
1344.6887 1344.6759 9.4827 329 341 (R)(G)
1368.6857 1368.6721 9.9542 187 197 (R)TEMENEFVLIK(K) 1Met-ox
1412.7256 1412.6844 29.1898 274 285 (R)(Y)
1419.7498 1419.7484 1.0428 214 225 (R)(Q)
1428.6961 1428.6793 11.7370 274 285 (R)SRAEAESMYQIK(Y) 1Met-ox
1566.7756 1566.7950 -12.4069 370 381 (R)QLREYQELMNVK(L) 1Met-ox
2775.4679 2775.4980 -10.8432 109 130 (K)(T)

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m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
1079.5447	1079.5373	6.8149	226	233	(R)(E)
1129.5942	1129.6217	-24.3695	342	352	(R)(L)
1129.5942	1129.6217	-24.3695	353	362	(K)(A)
1169.5292	1169.5512	-18.8863	276	285	(R)(Y)
1169.5292	1169.5512	-18.8863	373	381	(R)EYQELMNVK(L)	1Met-ox
1277.7115	1277.7105	0.7558	382	392	(K)(K)
1344.6887	1344.6759	9.4827	329	341	(R)(G)
1368.6857	1368.6721	9.9542	187	197	(R)TEMENEFVLIK(K)	1Met-ox
1412.7256	1412.6844	29.1898	274	285	(R)(Y)
1419.7498	1419.7484	1.0428	214	225	(R)(Q)
1428.6961	1428.6793	11.7370	274	285	(R)SRAEAESMYQIK(Y)	1Met-ox
1566.7756	1566.7950	-12.4069	370	381	(R)QLREYQELMNVK(L)	1Met-ox
2775.4679	2775.4980	-10.8432	109	130	(K)(T)

The matched peptides cover 25% (122/483AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 16.4 Mean Error = -3.1373 ppm Data Tolerance = 33.0232 ppm Mean Missed Cleavages = 0.5

4. 5/67 matches (7%). 49229.9 Da, pI = 5.89. Acc. # P31943. HUMAN. HETEROGENEOUS NUCLEAR RIBONUCLEOPROTEIN H (HNRNP H).

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
1079.5447 1079.5533 -7.9520 225 233 (R)(G)
1518.7237 1518.7011 14.9037 17 29 (R)(F)
1684.7495 1684.7679 -10.9699 99 114 (K)(L)
2107.0245 2106.9831 19.6347 50 68 (R)(L)
2142.0138 2142.0256 -5.5270 356 375 (R)(Y)

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m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)
1079.5447	1079.5533	-7.9520	225	233	(R)(G)
1518.7237	1518.7011	14.9037	17	29	(R)(F)
1684.7495	1684.7679	-10.9699	99	114	(K)(L)
2107.0245	2106.9831	19.6347	50	68	(R)(L)
2142.0138	2142.0256	-5.5270	356	375	(R)(Y)

The matched peptides cover 17% (77/449AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 7.5 Mean Error = 2.0179 ppm Data Tolerance = 28.3091 ppm Mean Missed Cleavages = 0.4

5. 5/67 matches (7%). 66018.2 Da, pI = 8.16. Acc. # P04264. HUMAN. KERATIN, TYPE II CYTOSKELETAL 1 (CYTOKERATIN 1) (K1) (CK 1) (67 KDA CYTOKERATIN) (HAIR ALPHA PROTEIN).

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
973.5368 973.5318 5.1280 396 403 (K)(V)
1179.6023 1179.6010 1.1216 377 386 (K)(H)
1277.7115 1277.7105 0.7558 473 483 (K)(T)
1475.7646 1475.7858 -14.3453 200 211 (R)(W)
1475.7646 1475.7494 10.3100 212 223 (K)(T)
1993.9732 1993.9772 -2.0312 224 239 (R)(R)

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m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)
973.5368	973.5318	5.1280	396	403	(K)(V)
1179.6023	1179.6010	1.1216	377	386	(K)(H)
1277.7115	1277.7105	0.7558	473	483	(K)(T)
1475.7646	1475.7858	-14.3453	200	211	(R)(W)
1475.7646	1475.7494	10.3100	212	223	(K)(T)
1993.9732	1993.9772	-2.0312	224	239	(R)(R)

The matched peptides cover 10% (69/644AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 7.5 Mean Error = 0.1565 ppm Data Tolerance = 16.5937 ppm Mean Missed Cleavages = 0.0