MS-Fit Search Results

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Sample ID (comment): HSG069
Database searched: SwissProt.7.2.2001
Molecular weight search (1000 - 100000 Da) selects 93834 entries.
Full pI range: 99359 entries.
Species search ( HUMAN MOUSE ) selects 11676 entries.
Combined molecular weight, pI and species searches select 10399 entries.
Pre searches select 10399 entries.
MS-Fit search selects 1077 entries (results displayed for top 5 matches).

Parameters Used in Search

Considered modifications: | Peptide N-terminal Gln to pyroGlu | Oxidation of M | Protein N-terminus Acetylated |

4	50.000 ppm	monoisotopic	Trypsin	3	acrylamide	Hydrogen (H)	Free Acid (O H)
Min. # Peptides to Match	Peptide Mass Tolerance (+/-)	Peptide Masses are	Digest Used	Max. # Missed Cleavages	Cysteines Modified by	Peptide N terminus	Peptide C terminus

Result Summary

Rank	MOWSE Score	# (%) Masses Matched	Protein MW (Da)/pI	Species	Protein Name
1	2.25e+015	29/61 (47%)	56560.4 / 5.26	HUMAN	ATP SYNTHASE BETA CHAIN, MITOCHONDRIAL PRECURSOR
2	3.27e+012	25/61 (40%)	56380.1 / 5.14	MOUSE	ATP SYNTHASE BETA CHAIN, MITOCHONDRIAL PRECURSOR
3	1.07e+003	12/61 (19%)	86564.7 / 5.57	HUMAN	INHIBITOR OF NUCLEAR FACTOR KAPPA B KINASE BETA SUBUNIT (I-KAPPA-B-KINASE BETA) (IKBKB) (IKK-BETA) (IKK-B) (I-KAPPA-B KINASE 2) (IKK2) (NUCLEAR FACTOR NF-KAPPA-B INHIBITOR KINASE BETA) (NFKBIKB)
4	926	9/61 (14%)	86691.0 / 6.03	MOUSE	INHIBITOR OF NUCLEAR FACTOR KAPPA B KINASE BETA SUBUNIT (I-KAPPA-B-KINASE BETA) (IKBKB) (IKK-BETA) (IKK-B) (I-KAPPA-B KINASE 2) (IKK2) (NUCLEAR FACTOR NF-KAPPA-B INHIBITOR KINASE BETA) (NFKBIKB)
5	455	5/61 (8%)	33150.1 / 5.61	HUMAN	C6.1A PROTEIN

Detailed Results

1. 29/61 matches (47%). 56560.4 Da, pI = 5.26. Acc. # P06576. HUMAN. ATP SYNTHASE BETA CHAIN, MITOCHONDRIAL PRECURSOR.

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
975.5368	975.5627	-26.5877	202	212	(K)(T)
1038.5728	1038.5948	-21.1164	134	143	(K)(I)
1088.5895	1088.6356	-42.2947	189	198	(K)(G)
1262.6008	1262.6415	-32.2408	110	121	(R)(G)
1278.6434	1278.6364	5.4940	110	121	(R)TIAMDGTEGLVR(G)	1Met-ox
1385.6873	1385.7099	-16.2685	144	155	(R)(G)
1401.6826	1401.7048	-15.8450	144	155	(R)IMNVIGEPIDER(G)	1Met-ox
1406.6577	1406.6817	-17.0251	226	239	(K)(T)
1435.7441	1435.7545	-7.2334	311	324	(R)(I)
1439.7691	1439.7898	-14.3745	282	294	(R)(D)
1457.7918	1457.8402	-33.1671	213	225	(K)(A)
1473.7982	1473.8351	-25.0333	213	225	(K)TVLIMELINNVAK(A)	1Met-ox
1601.7866	1601.8110	-15.2480	265	279	(K)(A)
1617.7855	1617.8059	-12.6103	265	279	(K)VALVYGQMNEPPGAR(A)	1Met-ox
1650.8964	1650.9179	-13.0372	95	109	(R)(T)
1815.8156	1815.8700	-29.9107	407	422	(R)(G)
1831.8122	1831.8649	-28.7328	407	422	(R)IMDPNIVGSEHYDVAR(G)	1Met-ox
1919.0717	1919.0966	-12.9770	125	143	(K)(I)
1921.9773	1921.9660	5.9008	295	310	(R)(F)
1987.9964	1988.0340	-18.9375	388	406	(R)(I)
2022.9908	2023.0111	-10.0750	463	480	(R)(L)
2038.9729	2039.0061	-16.2478	463	480	(R)FLSQPFQVAEVFTGHMGK(L)	1Met-ox
2060.9297	2060.9963	-32.3162	242	259	(R)(D)
2076.9746	2076.9912	-7.9886	242	259	(R)EGNDLYHEMIESGVINLK(D)	1Met-ox
2266.0251	2266.0848	-26.3168	325	345	(R)(I)
2282.0943	2282.0797	6.4088	325	345	(R)IPSAVGYQPTLATDMGTMQER(I)	1Met-ox
2298.0470	2298.0746	-12.0128	325	345	(R)IPSAVGYQPTLATDMGTMQER(I)	2Met-ox
2318.1113	2318.1451	-14.5643	240	259	(R)(D)
2334.1269	2334.1400	-5.6058	240	259	(R)TREGNDLYHEMIESGVINLK(D)	1Met-ox

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Click individual mass to do a non-specific cleavage search.

The matched peptides cover 48% (258/529AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 47.5 Mean Error = -16.8953 ppm Data Tolerance = 23.7943 ppm Mean Missed Cleavages = 0.1

2. 25/61 matches (40%). 56380.1 Da, pI = 5.14. Acc. # P56480. MOUSE. ATP SYNTHASE BETA CHAIN, MITOCHONDRIAL PRECURSOR.

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
975.5368 975.5627 -26.5877 202 212 (K)(T)
1038.5728 1038.5948 -21.1164 134 143 (K)(I)
1088.5895 1088.6356 -42.2947 189 198 (K)(G)
1262.6008 1262.6415 -32.2408 110 121 (R)(G)
1278.6434 1278.6364 5.4940 110 121 (R)TIAMDGTEGLVR(G) 1Met-ox
1385.6873 1385.7099 -16.2685 144 155 (R)(G)
1401.6826 1401.7048 -15.8450 144 155 (R)IMNVIGEPIDER(G) 1Met-ox
1435.7441 1435.7545 -7.2334 311 324 (R)(I)
1439.7691 1439.7898 -14.3745 282 294 (R)(D)
1457.7918 1457.8402 -33.1671 213 225 (K)(A)
1473.7982 1473.8351 -25.0333 213 225 (K)TVLIMELINNVAK(A) 1Met-ox
1650.8964 1650.9179 -13.0372 95 109 (R)(T)
1815.8156 1815.9063 -49.9472 265 281 (K)VALVYGQMNEPPGAPTR(V) 1Met-ox
1919.0717 1919.0966 -12.9770 125 143 (K)(I)
1921.9773 1921.9660 5.9008 295 310 (R)(F)
1987.9964 1988.0340 -18.9375 388 406 (R)(I)
2022.9908 2023.0111 -10.0750 463 480 (R)(L)
2038.9729 2039.0061 -16.2478 463 480 (R)FLSQPFQVAEVFTGHMGK(L) 1Met-ox
2060.9297 2060.9963 -32.3162 242 259 (R)(D)
2076.9746 2076.9912 -7.9886 242 259 (R)EGNDLYHEMIESGVINLK(D) 1Met-ox
2266.0251 2266.0848 -26.3168 325 345 (R)(I)
2282.0943 2282.0797 6.4088 325 345 (R)IPSAVGYQPTLATDMGTMQER(I) 1Met-ox
2298.0470 2298.0746 -12.0128 325 345 (R)IPSAVGYQPTLATDMGTMQER(I) 2Met-ox
2318.1113 2318.1451 -14.5643 240 259 (K)(D)
2318.1113 2318.1451 -14.5643 260 281 (K)DATSKVALVYGQMNEPPGAPTR(V) 1Met-ox
2334.1269 2334.1400 -5.6058 240 259 (K)TREGNDLYHEMIESGVINLK(D) 1Met-ox

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Click individual mass to do a non-specific cleavage search.

The matched peptides cover 44% (235/529AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 41.0 Mean Error = -17.3442 ppm Data Tolerance = 27.4960 ppm Mean Missed Cleavages = 0.2

3. 12/61 matches (19%). 86564.7 Da, pI = 5.57. Acc. # O14920. HUMAN. INHIBITOR OF NUCLEAR FACTOR KAPPA B KINASE BETA SUBUNIT (I-KAPPA-B-KINASE BETA) (IKBKB) (IKK-BETA) (IKK-B) (I-KAPPA-B KINASE 2) (IKK2) (NUCLEAR FACTOR NF-KAPPA-B INHIBITOR KINASE BETA) (NFKBIKB).

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
1151.5197 1151.5003 16.8809 583 592 (R)(L)
1262.6008 1262.6390 -30.2589 58 66 (R)(R)
1278.6434 1278.6339 7.4511 58 66 (R)WCLEIQIMR(R) 1Met-ox
1439.7691 1439.6987 48.9583 470 482 (K)NSMASMSQQLKAK(L) 1Met-ox
1457.7918 1457.7396 35.8474 45 55 (K)(E)
1483.7028 1483.7071 -2.8827 468 480 (K)(A)
1566.7420 1566.6818 38.3851 580 592 (R)DQRTEGDSQEMVR(L) 1Met-ox
1601.7866 1601.7205 41.2713 107 118 (K)(E)
2060.9297 2060.9783 -23.6112 576 592 (R)(L)
2076.9746 2076.9733 0.6496 576 592 (R)EKPRDQRTEGDSQEMVR(L) 1Met-ox
2318.1113 2318.1280 -7.1994 442 460 (K)(N)
2334.1269 2334.1229 1.7087 442 460 (K)EDCNRLQQGQRAAMMNLLR(N) 1Met-ox

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Click individual mass to do a non-specific cleavage search.

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
1151.5197	1151.5003	16.8809	583	592	(R)(L)
1262.6008	1262.6390	-30.2589	58	66	(R)(R)
1278.6434	1278.6339	7.4511	58	66	(R)WCLEIQIMR(R)	1Met-ox
1439.7691	1439.6987	48.9583	470	482	(K)NSMASMSQQLKAK(L)	1Met-ox
1457.7918	1457.7396	35.8474	45	55	(K)(E)
1483.7028	1483.7071	-2.8827	468	480	(K)(A)
1566.7420	1566.6818	38.3851	580	592	(R)DQRTEGDSQEMVR(L)	1Met-ox
1601.7866	1601.7205	41.2713	107	118	(K)(E)
2060.9297	2060.9783	-23.6112	576	592	(R)(L)
2076.9746	2076.9733	0.6496	576	592	(R)EKPRDQRTEGDSQEMVR(L)	1Met-ox
2318.1113	2318.1280	-7.1994	442	460	(K)(N)
2334.1269	2334.1229	1.7087	442	460	(K)EDCNRLQQGQRAAMMNLLR(N)	1Met-ox

The matched peptides cover 10% (83/756AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 19.7 Mean Error = 10.6000 ppm Data Tolerance = 51.8298 ppm Mean Missed Cleavages = 1.1

4. 9/61 matches (14%). 86691.0 Da, pI = 6.03. Acc. # O88351. MOUSE. INHIBITOR OF NUCLEAR FACTOR KAPPA B KINASE BETA SUBUNIT (I-KAPPA-B-KINASE BETA) (IKBKB) (IKK-BETA) (IKK-B) (I-KAPPA-B KINASE 2) (IKK2) (NUCLEAR FACTOR NF-KAPPA-B INHIBITOR KINASE BETA) (NFKBIKB).

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
1151.5197 1151.5003 16.8809 583 592 (R)(L)
1262.6008 1262.6390 -30.2589 58 66 (R)(R)
1278.6434 1278.6339 7.4511 58 66 (R)WCLEIQIMR(R) 1Met-ox
1495.7670 1495.7658 0.8345 32 44 (R)(Q)
1566.7420 1566.6818 38.3851 580 592 (R)DQRTEGDSQEMVR(L) 1Met-ox
1601.7866 1601.7205 41.2713 107 118 (R)(E)
1987.9964 1988.0745 -39.2624 483 498 (K)(E)
2060.9297 2060.9783 -23.6112 576 592 (R)(L)
2076.9746 2076.9733 0.6496 576 592 (R)EKPRDQRTEGDSQEMVR(L) 1Met-ox

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Click individual mass to do a non-specific cleavage search.

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
1151.5197	1151.5003	16.8809	583	592	(R)(L)
1262.6008	1262.6390	-30.2589	58	66	(R)(R)
1278.6434	1278.6339	7.4511	58	66	(R)WCLEIQIMR(R)	1Met-ox
1495.7670	1495.7658	0.8345	32	44	(R)(Q)
1566.7420	1566.6818	38.3851	580	592	(R)DQRTEGDSQEMVR(L)	1Met-ox
1601.7866	1601.7205	41.2713	107	118	(R)(E)
1987.9964	1988.0745	-39.2624	483	498	(K)(E)
2060.9297	2060.9783	-23.6112	576	592	(R)(L)
2076.9746	2076.9733	0.6496	576	592	(R)EKPRDQRTEGDSQEMVR(L)	1Met-ox

The matched peptides cover 8% (67/757AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 14.8 Mean Error = 1.3711 ppm Data Tolerance = 57.0791 ppm Mean Missed Cleavages = 0.8

5. 5/61 matches (8%). 33150.1 Da, pI = 5.61. Acc. # P46736. HUMAN. C6.1A PROTEIN.

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
1307.6646 1307.6041 46.3237 281 291 (K)(-)
2282.0943 2282.0281 29.0386 32 51 (K)(F)
2298.0470 2298.0230 10.4591 32 51 (K)EEVMGLCIGELNDDTRSDSK(F) 1Met-ox
2318.1113 2318.1273 -6.9037 246 265 (K)(L)
2334.1269 2334.1222 2.0024 246 265 (K)NLCSQMSAVSGPLLQWLEDR(L) 1Met-ox

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Click individual mass to do a non-specific cleavage search.

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
1307.6646	1307.6041	46.3237	281	291	(K)(-)
2282.0943	2282.0281	29.0386	32	51	(K)(F)
2298.0470	2298.0230	10.4591	32	51	(K)EEVMGLCIGELNDDTRSDSK(F)	1Met-ox
2318.1113	2318.1273	-6.9037	246	265	(K)(L)
2334.1269	2334.1222	2.0024	246	265	(K)NLCSQMSAVSGPLLQWLEDR(L)	1Met-ox

The matched peptides cover 17% (51/291AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 8.2 Mean Error = 16.1840 ppm Data Tolerance = 42.9019 ppm Mean Missed Cleavages = 0.4