MS-Fit Search Results

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Sample ID (comment): HSG070
Database searched: SwissProt.8.20.2001
Molecular weight search (1000 - 100000 Da) selects 94194 entries.
Full pI range: 99754 entries.
Species search ( HUMAN MOUSE ) selects 11713 entries.
Combined molecular weight, pI and species searches select 10428 entries.
Pre searches select 10428 entries.
MS-Fit search selects 644 entries (results displayed for top 5 matches).

Parameters Used in Search

Considered modifications: | Oxidation of M | Protein N-terminus Acetylated | Acrylamide Modified Cys |

4	70.000 ppm	monoisotopic	Trypsin	1	carbamidomethylation	Hydrogen (H)	Free Acid (O H)
Min. # Peptides to Match	Peptide Mass Tolerance (+/-)	Peptide Masses are	Digest Used	Max. # Missed Cleavages	Cysteines Modified by	Peptide N terminus	Peptide C terminus

Result Summary

Rank	MOWSE Score	# (%) Masses Matched	Protein MW (Da)/pI	Species	Protein Name
1	7.3e+007	11/50 (22%)	56560.4 / 5.26	HUMAN	ATP SYNTHASE BETA CHAIN, MITOCHONDRIAL PRECURSOR
2	4.34e+005	9/50 (18%)	56380.1 / 5.14	MOUSE	ATP SYNTHASE BETA CHAIN, MITOCHONDRIAL PRECURSOR
3	2.75e+004	6/50 (12%)	60071.6 / 8.24	HUMAN	UDP-GLUCURONOSYLTRANSFERASE 1-5 PRECURSOR, MICROSOMAL (UDPGT) (UGT-1E) (UGT1*5) (UGT1-05) (UGT1.5) (UGT1A5) (UGT1E)
4	1.77e+004	7/50 (14%)	36323.7 / 7.55	HUMAN	RAS-RELATED PROTEIN RAB-36
5	8e+003	6/50 (12%)	44552.6 / 6.10	HUMAN	CATHEPSIN D PRECURSOR

Detailed Results

1. 11/50 matches (22%). 56560.4 Da, pI = 5.26. Acc. # P06576. HUMAN. ATP SYNTHASE BETA CHAIN, MITOCHONDRIAL PRECURSOR.

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)
1385.7313	1385.7099	15.4596	144	155	(R)(G)
1406.7253	1406.6817	31.0199	226	239	(K)(T)
1435.7565	1435.7545	1.3697	311	324	(R)(I)
1439.8220	1439.7898	22.3322	282	294	(R)(D)
1601.8582	1601.8110	29.4557	265	279	(K)(A)
1650.9769	1650.9179	35.7532	95	109	(R)(T)
1815.8970	1815.8700	14.8877	407	422	(R)(G)
1919.1451	1919.0966	25.2655	125	143	(K)(I)
1988.1326	1988.0340	49.5528	388	406	(R)(I)
2266.1835	2266.0848	43.5822	325	345	(R)(I)
2318.2661	2318.1451	52.1994	240	259	(R)(D)

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The matched peptides cover 33% (178/529AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 22.0 Mean Error = 29.1707 ppm Data Tolerance = 31.1588 ppm Mean Missed Cleavages = 0.2

2. 9/50 matches (18%). 56380.1 Da, pI = 5.14. Acc. # P56480. MOUSE. ATP SYNTHASE BETA CHAIN, MITOCHONDRIAL PRECURSOR.

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
1385.7313 1385.7099 15.4596 144 155 (R)(G)
1435.7565 1435.7545 1.3697 311 324 (R)(I)
1439.8220 1439.7898 22.3322 282 294 (R)(D)
1650.9769 1650.9179 35.7532 95 109 (R)(T)
1815.8970 1815.9063 -5.1497 265 281 (K)VALVYGQMNEPPGAPTR(V) 1Met-ox
1919.1451 1919.0966 25.2655 125 143 (K)(I)
1988.1326 1988.0340 49.5528 388 406 (R)(I)
2266.1835 2266.0848 43.5822 325 345 (R)(I)
2318.2661 2318.1451 52.1994 240 259 (K)(D)
2318.2661 2318.1451 52.1994 260 281 (K)DATSKVALVYGQMNEPPGAPTR(V) 1Met-ox

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The matched peptides cover 29% (155/529AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 18.0 Mean Error = 29.2564 ppm Data Tolerance = 41.7815 ppm Mean Missed Cleavages = 0.3

3. 6/50 matches (12%). 60071.6 Da, pI = 8.24. Acc. # P35504. HUMAN. UDP-GLUCURONOSYLTRANSFERASE 1-5 PRECURSOR, MICROSOMAL (UDPGT) (UGT-1E) (UGT1*5) (UGT1-05) (UGT1.5) (UGT1A5) (UGT1E).

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
1543.7832 1543.7911 -5.1253 114 126 (R)(S)
1559.7967 1559.7860 6.8080 114 126 (R)MAIMNNMSLIIHR(S) 1Met-ox
2144.1033 2144.1214 -8.4452 92 109 (R)(F)
2318.2661 2318.2007 28.1863 450 468 (K)(H)
2550.3945 2550.3179 30.0274 92 112 (R)LLLGHTQSFFETEHLLMKFSR(R) 1Met-ox
2676.4656 2676.3972 25.5461 140 163 (R)HLHATSFDVVLTDPFHLCAAVLAK(Y) 1Cys-am

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m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
1543.7832	1543.7911	-5.1253	114	126	(R)(S)
1559.7967	1559.7860	6.8080	114	126	(R)MAIMNNMSLIIHR(S)	1Met-ox
2144.1033	2144.1214	-8.4452	92	109	(R)(F)
2318.2661	2318.2007	28.1863	450	468	(K)(H)
2550.3945	2550.3179	30.0274	92	112	(R)LLLGHTQSFFETEHLLMKFSR(R)	1Met-ox
2676.4656	2676.3972	25.5461	140	163	(R)HLHATSFDVVLTDPFHLCAAVLAK(Y)	1Cys-am

The matched peptides cover 14% (77/534AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 12.0 Mean Error = 12.8329 ppm Data Tolerance = 34.6936 ppm Mean Missed Cleavages = 0.2

4. 7/50 matches (14%). 36323.7 Da, pI = 7.55. Acc. # O95755. HUMAN. RAS-RELATED PROTEIN RAB-36.

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
1099.5885 1099.5359 47.8936 143 150 (R)FCKNVFDR(D) 1Cys-am
1481.7130 1481.8076 -63.8542 69 82 (R)(V)
1526.6676 1526.6923 -16.1331 101 113 (R)(R)
1788.9595 1788.9496 5.5561 283 298 (R)(Q)
2057.0624 2056.9650 47.3627 260 277 (R)(A)
2266.1835 2266.1396 19.4008 165 184 (R)(F)
2318.2661 2318.2104 23.9963 283 303 (R)(L)

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m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
1099.5885	1099.5359	47.8936	143	150	(R)FCKNVFDR(D)	1Cys-am
1481.7130	1481.8076	-63.8542	69	82	(R)(V)
1526.6676	1526.6923	-16.1331	101	113	(R)(R)
1788.9595	1788.9496	5.5561	283	298	(R)(Q)
2057.0624	2056.9650	47.3627	260	277	(R)(A)
2266.1835	2266.1396	19.4008	165	184	(R)(F)
2318.2661	2318.2104	23.9963	283	303	(R)(L)

The matched peptides cover 28% (94/333AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 14.0 Mean Error = 9.1746 ppm Data Tolerance = 78.6068 ppm Mean Missed Cleavages = 0.6

5. 6/50 matches (12%). 44552.6 Da, pI = 6.10. Acc. # P07339. HUMAN. CATHEPSIN D PRECURSOR.

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
1109.5285 1109.5955 -60.3775 257 266 (K)(K)
1263.6945 1263.6697 19.6205 174 184 (K)(Q)
1419.6976 1419.7571 -41.8793 112 122 (K)LLDIACWIHHK(Y) 1Cys-am
1601.8582 1601.8328 15.8576 223 235 (K)(D)
2266.1835 2266.2058 -9.8318 185 205 (K)(I)
2348.2383 2348.1114 54.0572 288 309 (K)EGCEAIVDTGTSLMVGPVDEVR(E) 1Cys-am

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m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
1109.5285	1109.5955	-60.3775	257	266	(K)(K)
1263.6945	1263.6697	19.6205	174	184	(K)(Q)
1419.6976	1419.7571	-41.8793	112	122	(K)LLDIACWIHHK(Y)	1Cys-am
1601.8582	1601.8328	15.8576	223	235	(K)(D)
2266.1835	2266.2058	-9.8318	185	205	(K)(I)
2348.2383	2348.1114	54.0572	288	309	(K)EGCEAIVDTGTSLMVGPVDEVR(E)	1Cys-am

The matched peptides cover 21% (88/412AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 12.0 Mean Error = -3.7589 ppm Data Tolerance = 84.7104 ppm Mean Missed Cleavages = 0.3