MS-Fit Search Results

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Sample ID (comment): HSG072
Database searched: SwissProt.8.20.2001
Molecular weight search (1000 - 100000 Da) selects 94194 entries.
Full pI range: 99754 entries.
Species search ( HUMAN MOUSE ) selects 11713 entries.
Combined molecular weight, pI and species searches select 10428 entries.
Pre searches select 10428 entries.
MS-Fit search selects 340 entries (results displayed for top 5 matches).

Parameters Used in Search

Considered modifications: | Oxidation of M | Protein N-terminus Acetylated | Acrylamide Modified Cys |

4	75.000 ppm	monoisotopic	Trypsin	1	carbamidomethylation	Hydrogen (H)	Free Acid (O H)
Min. # Peptides to Match	Peptide Mass Tolerance (+/-)	Peptide Masses are	Digest Used	Max. # Missed Cleavages	Cysteines Modified by	Peptide N terminus	Peptide C terminus

Result Summary

Rank	MOWSE Score	# (%) Masses Matched	Protein MW (Da)/pI	Species	Protein Name
1	9.76e+011	22/45 (48%)	48106.1 / 4.97	HUMAN	KERATIN, TYPE I CYTOSKELETAL 17 (CYTOKERATIN 17) (K17) (CK 17) (39.1) (VERSION 1)
2	4.82e+004	9/45 (20%)	87193.1 / 8.92	MOUSE	CARNITINE O-PALMITOYLTRANSFERASE I, MITOCHONDRIAL LIVER ISOFORM (CPT I) (CPTI-L)
3	5.57e+003	10/45 (22%)	51622.0 / 5.09	HUMAN	KERATIN, TYPE I CYTOSKELETAL 14 (CYTOKERATIN 14) (K14) (CK 14)
4	2.67e+003	9/45 (20%)	41479.8 / 4.91	MOUSE	KERATIN, TYPE I CYTOSKELETAL 14 (CYTOKERATIN 14) (K14) (CK 14)
5	2.33e+003	6/45 (13%)	51050.6 / 5.32	HUMAN	KERATIN, TYPE I CYTOSKELETAL 16 (CYTOKERATIN 16) (K16) (CK 16)

Detailed Results

1. 22/45 matches (48%). 48106.1 Da, pI = 4.97. Acc. # Q04695. HUMAN. KERATIN, TYPE I CYTOSKELETAL 17 (CYTOKERATIN 17) (K17) (CK 17) (39.1) (VERSION 1).

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
775.4406	775.4136	34.7829	252	257	(R)(D)
807.3895	807.4001	-13.1524	164	170	(R)(T)
989.5423	989.5380	4.3356	298	305	(K)(T)
994.4591	994.4270	32.2534	130	136	(R)(T)
1029.6237	1029.5944	28.3821	193	201	(R)(A)
1036.5656	1036.5328	31.5776	116	122	(K)(Q)
1036.5656	1036.5427	22.0302	164	172	(R)(F)
1048.5528	1048.5210	30.4062	31	41	(R)LSGGLGAGSCR(L)	1Cys-am
1144.5384	1144.4951	37.8133	270	278	(K)(T)
1222.7129	1222.6432	57.0468	171	180	(R)(L)
1242.6201	1242.5537	53.4575	221	230	(K)(G)
1342.7856	1342.7443	30.7377	181	192	(R)(V)
1379.7910	1379.7283	45.4541	375	385	(K)(R)
1403.7332	1403.6767	40.2550	322	334	(K)(Y)
1438.7612	1438.7000	42.4939	252	262	(R)(M)
1454.7474	1454.6950	36.0607	252	262	(R)ILNEMRDQYEK(M)	1Met-ox
1500.6785	1500.6099	45.7198	358	368	(R)CEMEQQNQEYK(I)	1Cys-am
1516.7668	1516.7647	1.3609	387	399	(R)(K)
1849.9795	1849.9403	21.1798	306	321	(R)(A)
1866.0120	1865.9353	41.1315	306	321	(R)TMQALEIELQSQLSMK(A)	1Met-ox
2068.2335	2068.1402	45.0759	145	163	(K)(L)
2114.1124	2114.0188	44.2607	231	251	(R)(I)
2130.1189	2130.0137	49.3881	231	251	(R)GQVGGEINVEMDAAPGVDLSR(I)	1Met-ox

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The matched peptides cover 47% (205/432AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 48.9 Mean Error = 33.1327 ppm Data Tolerance = 33.9722 ppm Mean Missed Cleavages = 0.3

2. 9/45 matches (20%). 87193.1 Da, pI = 8.92. Acc. # P97742. MOUSE. CARNITINE O-PALMITOYLTRANSFERASE I, MITOCHONDRIAL LIVER ISOFORM (CPT I) (CPTI-L).

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
1036.5656 1036.5904 -23.9088 500 508 (K)(L)
1222.7129 1222.6440 56.3365 137 146 (R)(V)
1342.7856 1342.6942 68.0678 21 31 (K)QICLSGLHSWK(K) 1Cys-am
1454.7474 1454.7327 10.1286 626 636 (K)(L)
1516.7668 1516.8204 -35.3372 443 454 (R)(N)
2114.1124 2114.1221 -4.5954 147 164 (K)(L)
2130.1189 2130.1170 0.8986 147 164 (K)VFSGRKPMLYSFQTSLPR(L) 1Met-ox
2278.2861 2278.2456 17.7842 94 114 (K)(Y)
2294.2810 2294.2405 17.6521 94 114 (K)NIVSGVLFGTGLWVAIIMTMR(Y) 1Met-ox

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m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
1036.5656	1036.5904	-23.9088	500	508	(K)(L)
1222.7129	1222.6440	56.3365	137	146	(R)(V)
1342.7856	1342.6942	68.0678	21	31	(K)QICLSGLHSWK(K)	1Cys-am
1454.7474	1454.7327	10.1286	626	636	(K)(L)
1516.7668	1516.8204	-35.3372	443	454	(R)(N)
2114.1124	2114.1221	-4.5954	147	164	(K)(L)
2130.1189	2130.1170	0.8986	147	164	(K)VFSGRKPMLYSFQTSLPR(L)	1Met-ox
2278.2861	2278.2456	17.7842	94	114	(K)(Y)
2294.2810	2294.2405	17.6521	94	114	(K)NIVSGVLFGTGLWVAIIMTMR(Y)	1Met-ox

The matched peptides cover 12% (92/764AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 20.0 Mean Error = 11.8918 ppm Data Tolerance = 67.4797 ppm Mean Missed Cleavages = 0.6

3. 10/45 matches (22%). 51622.0 Da, pI = 5.09. Acc. # P02533. HUMAN. KERATIN, TYPE I CYTOSKELETAL 14 (CYTOKERATIN 14) (K14) (CK 14).

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
775.4406 775.4136 34.7829 283 288 (R)(D)
807.3895 807.4001 -13.1524 195 201 (R)(T)
989.5423 989.5380 4.3356 329 336 (K)(T)
1029.6237 1029.5944 28.3821 224 232 (R)(A)
1036.5656 1036.5328 31.5776 147 153 (K)(Q)
1036.5656 1036.5427 22.0302 195 203 (R)(Y)
1242.6201 1242.5537 53.4575 252 261 (K)(G)
1379.7910 1379.7283 45.4541 406 416 (K)(R)
1438.7612 1438.7000 42.4939 283 293 (R)(M)
1454.7474 1454.6950 36.0607 283 293 (R)ILNEMRDQYEK(M) 1Met-ox
1500.6785 1500.6099 45.7198 389 399 (R)CEMEQQNQEYK(I) 1Cys-am

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m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
775.4406	775.4136	34.7829	283	288	(R)(D)
807.3895	807.4001	-13.1524	195	201	(R)(T)
989.5423	989.5380	4.3356	329	336	(K)(T)
1029.6237	1029.5944	28.3821	224	232	(R)(A)
1036.5656	1036.5328	31.5776	147	153	(K)(Q)
1036.5656	1036.5427	22.0302	195	203	(R)(Y)
1242.6201	1242.5537	53.4575	252	261	(K)(G)
1379.7910	1379.7283	45.4541	406	416	(K)(R)
1438.7612	1438.7000	42.4939	283	293	(R)(M)
1454.7474	1454.6950	36.0607	283	293	(R)ILNEMRDQYEK(M)	1Met-ox
1500.6785	1500.6099	45.7198	389	399	(R)CEMEQQNQEYK(I)	1Cys-am

The matched peptides cover 16% (76/472AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 22.2 Mean Error = 30.1038 ppm Data Tolerance = 39.1980 ppm Mean Missed Cleavages = 0.5

4. 9/45 matches (20%). 41479.8 Da, pI = 4.91. Acc. # Q61781. MOUSE. KERATIN, TYPE I CYTOSKELETAL 14 (CYTOKERATIN 14) (K14) (CK 14).

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
775.4406 775.4136 34.7829 164 169 (R)(D)
807.3895 807.4001 -13.1524 76 82 (R)(T)
989.5423 989.5380 4.3356 210 217 (K)(T)
1029.6237 1029.5944 28.3821 105 113 (R)(A)
1036.5656 1036.5328 31.5776 28 34 (K)(Q)
1036.5656 1036.5427 22.0302 76 84 (R)(F)
1379.7910 1379.7283 45.4541 287 297 (K)(R)
1438.7612 1438.7000 42.4939 164 174 (R)(M)
1454.7474 1454.6950 36.0607 164 174 (R)ILNEMRDQYEK(M) 1Met-ox
1500.6785 1500.6099 45.7198 270 280 (R)CEMEQQNQEYK(I) 1Cys-am

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m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
775.4406	775.4136	34.7829	164	169	(R)(D)
807.3895	807.4001	-13.1524	76	82	(R)(T)
989.5423	989.5380	4.3356	210	217	(K)(T)
1029.6237	1029.5944	28.3821	105	113	(R)(A)
1036.5656	1036.5328	31.5776	28	34	(K)(Q)
1036.5656	1036.5427	22.0302	76	84	(R)(F)
1379.7910	1379.7283	45.4541	287	297	(K)(R)
1438.7612	1438.7000	42.4939	164	174	(R)(M)
1454.7474	1454.6950	36.0607	164	174	(R)ILNEMRDQYEK(M)	1Met-ox
1500.6785	1500.6099	45.7198	270	280	(R)CEMEQQNQEYK(I)	1Cys-am

The matched peptides cover 18% (66/359AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 20.0 Mean Error = 27.7684 ppm Data Tolerance = 37.9547 ppm Mean Missed Cleavages = 0.6

5. 6/45 matches (13%). 51050.6 Da, pI = 5.32. Acc. # P30654. HUMAN. KERATIN, TYPE I CYTOSKELETAL 16 (CYTOKERATIN 16) (K16) (CK 16).

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
807.3895 807.4001 -13.1524 198 204 (R)(T)
1029.6237 1029.5944 28.3821 227 235 (R)(T)
1036.5656 1036.5468 18.1492 304 312 (R)(T)
1379.7910 1379.7283 45.4541 409 419 (K)(R)
1849.9795 1849.9734 3.3334 340 355 (R)(A)
1866.0120 1865.9683 23.4377 340 355 (R)VFQGLEMELQSQLSIK(A) 1Met-ox

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m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
807.3895	807.4001	-13.1524	198	204	(R)(T)
1029.6237	1029.5944	28.3821	227	235	(R)(T)
1036.5656	1036.5468	18.1492	304	312	(R)(T)
1379.7910	1379.7283	45.4541	409	419	(K)(R)
1849.9795	1849.9734	3.3334	340	355	(R)(A)
1866.0120	1865.9683	23.4377	340	355	(R)VFQGLEMELQSQLSIK(A)	1Met-ox

The matched peptides cover 10% (52/474AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 13.3 Mean Error = 17.6007 ppm Data Tolerance = 40.7503 ppm Mean Missed Cleavages = 0.2