MS-Fit Search Results

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Sample ID (comment): HSG073
Database searched: SwissProt.8.20.2001
Molecular weight search (1000 - 100000 Da) selects 94194 entries.
Full pI range: 99754 entries.
Species search ( HUMAN MOUSE ) selects 11713 entries.
Combined molecular weight, pI and species searches select 10428 entries.
Pre searches select 10428 entries.
MS-Fit search selects 621 entries (results displayed for top 5 matches).

Parameters Used in Search

Considered modifications: | Oxidation of M | Protein N-terminus Acetylated | Acrylamide Modified Cys |

4	150.000 ppm	monoisotopic	Trypsin	1	carbamidomethylation	Hydrogen (H)	Free Acid (O H)
Min. # Peptides to Match	Peptide Mass Tolerance (+/-)	Peptide Masses are	Digest Used	Max. # Missed Cleavages	Cysteines Modified by	Peptide N terminus	Peptide C terminus

Result Summary

Rank	MOWSE Score	# (%) Masses Matched	Protein MW (Da)/pI	Species	Protein Name
1	3.07e+011	19/40 (47%)	45672.3 / 5.38	HUMAN	HETEROGENEOUS NUCLEAR RIBONUCLEOPROTEIN F (HNRNP F)
2	3.42e+004	7/40 (17%)	88413.3 / 6.15	HUMAN	CAMP AND CAMP-INHIBITED CGMP 3',5'-CYCLIC PHOSPHODIESTERASE 10A
3	1.85e+004	7/40 (17%)	46394.6 / 5.33	HUMAN	EUKARYOTIC INITIATION FACTOR 4A-II (EIF-4A-II) (EIF4A-II)
4	1.85e+004	7/40 (17%)	46402.7 / 5.33	MOUSE	EUKARYOTIC INITIATION FACTOR 4A-II (EIF-4A-II) (EIF4A-II)
5	1.63e+004	12/40 (30%)	86564.7 / 5.57	HUMAN	INHIBITOR OF NUCLEAR FACTOR KAPPA B KINASE BETA SUBUNIT (I-KAPPA-B-KINASE BETA) (IKBKB) (IKK-BETA) (IKK-B) (I-KAPPA-B KINASE 2) (IKK2) (NUCLEAR FACTOR NF-KAPPA-B INHIBITOR KINASE BETA) (NFKBIKB)

Detailed Results

1. 19/40 matches (47%). 45672.3 Da, pI = 5.38. Acc. # P52597. HUMAN. HETEROGENEOUS NUCLEAR RIBONUCLEOPROTEIN F (HNRNP F).

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
798.5010	798.4402	76.1570	82	87	(R)(S)
798.5010	798.4402	76.1570	180	185	(R)(S)
834.4313	834.3957	42.5899	186	192	(K)(S)
1092.6770	1092.5802	88.5757	317	326	(R)(V)
1361.7964	1361.6775	87.3352	1	14	(-)MLGPEGGEGFVVK(L)	Acet N
1377.8012	1377.6724	93.4472	1	14	(-)MLGPEGGEGFVVK(L)	1Met-ox Acet N
1401.8234	1401.6949	91.6861	88	98	(K)(H)
1630.8665	1630.8627	2.3143	1	16	(-)MLGPEGGEGFVVKLR(G)	Acet N
1630.8665	1630.7210	89.2099	99	114	(K)(L)
1630.8665	1630.6994	102.4560	263	275	(R)DLSYCLSGMYDHR(Y)	1Cys-am
1646.8625	1646.8576	2.9606	1	16	(-)MLGPEGGEGFVVKLR(G)	1Met-ox Acet N
1646.8625	1646.6943	102.1296	263	275	(R)DLSYCLSGMYDHR(Y)	1Met-ox 1Cys-am
1709.9491	1709.7870	94.7911	53	68	(R)(M)
1868.1117	1867.9442	89.6747	151	167	(K)(A)
1935.1461	1934.9659	93.1076	201	217	(K)(R)
1951.1330	1950.9608	88.2316	201	217	(K)FMSVQRPGPYDRPGTAR(R)	1Met-ox
1997.1653	1996.9769	94.3577	300	316	(K)(V)
2192.2380	2192.0181	100.2906	327	347	(R)(D)
2208.2173	2208.0131	92.5131	327	347	(R)VTGEADVEFATHEEAVAAMSK(D)	1Met-ox
2212.2769	2211.9552	145.4542	276	294	(R)(G)
2226.2041	2225.9708	104.8082	276	294	(R)YGDSEFTVQSTTGHCVHMR(G)	1Cys-am
2242.1673	2241.9657	89.9075	276	294	(R)YGDSEFTVQSTTGHCVHMR(G)	1Met-ox 1Cys-am
2463.4155	2463.1462	109.3369	327	349	(R)(A)

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The matched peptides cover 46% (194/415AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 47.5 Mean Error = 85.1083 ppm Data Tolerance = 62.5335 ppm Mean Missed Cleavages = 0.2

2. 7/40 matches (17%). 88413.3 Da, pI = 6.15. Acc. # Q9Y233. HUMAN. CAMP AND CAMP-INHIBITED CGMP 3',5'-CYCLIC PHOSPHODIESTERASE 10A.

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
868.6112 868.4966 131.9070 501 507 (R)FIMSVKK(N) 1Met-ox
1566.8856 1566.7409 92.3861 423 434 (R)HSECIYRVTMEK(L) 1Cys-am
1630.8665 1630.8077 36.0476 337 351 (R)(F)
1692.8987 1692.8314 39.7350 545 558 (K)(G)
2212.2769 2212.0077 121.7121 403 420 (K)(I)
2226.2041 2226.0233 81.2166 403 420 (K)MFAVFCALALHCANMYHR(I) 1Cys-am
2242.1673 2242.0182 66.4845 403 420 (K)MFAVFCALALHCANMYHR(I) 1Met-ox 1Cys-am

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m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
868.6112	868.4966	131.9070	501	507	(R)FIMSVKK(N)	1Met-ox
1566.8856	1566.7409	92.3861	423	434	(R)HSECIYRVTMEK(L)	1Cys-am
1630.8665	1630.8077	36.0476	337	351	(R)(F)
1692.8987	1692.8314	39.7350	545	558	(K)(G)
2212.2769	2212.0077	121.7121	403	420	(K)(I)
2226.2041	2226.0233	81.2166	403	420	(K)MFAVFCALALHCANMYHR(I)	1Cys-am
2242.1673	2242.0182	66.4845	403	420	(K)MFAVFCALALHCANMYHR(I)	1Met-ox 1Cys-am

The matched peptides cover 8% (66/779AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 17.5 Mean Error = 81.3556 ppm Data Tolerance = 74.4402 ppm Mean Missed Cleavages = 0.3

3. 7/40 matches (17%). 46394.6 Da, pI = 5.33. Acc. # Q14240. HUMAN. EUKARYOTIC INITIATION FACTOR 4A-II (EIF-4A-II) (EIF4A-II).

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
1050.6335 1050.5519 77.7119 163 170 (R)(Y)
1068.6582 1068.5478 103.3298 249 256 (K)(E)
1401.8234 1401.6949 91.6861 286 296 (K)VDWLTEKMHAR(D) 1Met-ox
1555.8893 1555.7136 112.9435 179 191 (K)(G)
1828.0912 1827.9394 83.0760 47 62 (R)(A)
2144.3366 2144.1352 93.9701 336 354 (R)(E)
2463.4155 2463.1947 89.6500 120 142 (K)VILALGDYMGATCHACIGGTNVR(N) 1Cys-am

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m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
1050.6335	1050.5519	77.7119	163	170	(R)(Y)
1068.6582	1068.5478	103.3298	249	256	(K)(E)
1401.8234	1401.6949	91.6861	286	296	(K)VDWLTEKMHAR(D)	1Met-ox
1555.8893	1555.7136	112.9435	179	191	(K)(G)
1828.0912	1827.9394	83.0760	47	62	(R)(A)
2144.3366	2144.1352	93.9701	336	354	(R)(E)
2463.4155	2463.1947	89.6500	120	142	(K)VILALGDYMGATCHACIGGTNVR(N)	1Cys-am

The matched peptides cover 24% (98/407AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 17.5 Mean Error = 93.1953 ppm Data Tolerance = 23.8088 ppm Mean Missed Cleavages = 0.3

4. 7/40 matches (17%). 46402.7 Da, pI = 5.33. Acc. # P10630. MOUSE. EUKARYOTIC INITIATION FACTOR 4A-II (EIF-4A-II) (EIF4A-II).

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
1050.6335 1050.5519 77.7119 163 170 (R)(Y)
1068.6582 1068.5478 103.3298 249 256 (K)(E)
1401.8234 1401.6949 91.6861 286 296 (K)VDWLTEKMHAR(D) 1Met-ox
1555.8893 1555.7136 112.9435 179 191 (K)(G)
1828.0912 1827.9394 83.0760 47 62 (R)(A)
2144.3366 2144.1352 93.9701 336 354 (R)(E)
2463.4155 2463.1947 89.6500 120 142 (K)VILALGDYMGATCHACIGGTNVR(N) 1Cys-am

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m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
1050.6335	1050.5519	77.7119	163	170	(R)(Y)
1068.6582	1068.5478	103.3298	249	256	(K)(E)
1401.8234	1401.6949	91.6861	286	296	(K)VDWLTEKMHAR(D)	1Met-ox
1555.8893	1555.7136	112.9435	179	191	(K)(G)
1828.0912	1827.9394	83.0760	47	62	(R)(A)
2144.3366	2144.1352	93.9701	336	354	(R)(E)
2463.4155	2463.1947	89.6500	120	142	(K)VILALGDYMGATCHACIGGTNVR(N)	1Cys-am

The matched peptides cover 24% (98/407AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 17.5 Mean Error = 93.1953 ppm Data Tolerance = 23.8088 ppm Mean Missed Cleavages = 0.3

5. 12/40 matches (30%). 86564.7 Da, pI = 5.57. Acc. # O14920. HUMAN. INHIBITOR OF NUCLEAR FACTOR KAPPA B KINASE BETA SUBUNIT (I-KAPPA-B-KINASE BETA) (IKBKB) (IKK-BETA) (IKK-B) (I-KAPPA-B KINASE 2) (IKK2) (NUCLEAR FACTOR NF-KAPPA-B INHIBITOR KINASE BETA) (NFKBIKB).

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
798.5010 798.4950 7.4857 574 579 (R)(D)
822.3267 822.3780 -62.3577 461 467 (R)(M)
868.6112 868.4933 135.7888 481 487 (K)(T)
1361.7964 1361.6120 135.4884 667 680 (R)(L)
1377.8012 1377.6069 141.0417 667 680 (R)GPVSGSPDSMNASR(L) 1Met-ox
1383.7995 1383.8364 -26.6344 160 171 (R)(E)
1566.8856 1566.6818 130.0712 580 592 (R)DQRTEGDSQEMVR(L) 1Met-ox
1709.9491 1709.9491 -0.0368 428 441 (R)(E)
1935.1461 1934.8952 129.6699 516 531 (R)EMEQAVELCGRENEVK(L) 1Cys-am
1951.1330 1950.8901 124.4939 516 531 (R)EMEQAVELCGRENEVK(L) 1Met-ox 1Cys-am
2144.3366 2144.1572 83.7097 532 549 (K)(S)
2208.2173 2208.0647 69.1232 376 394 (K)(I)

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m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
798.5010	798.4950	7.4857	574	579	(R)(D)
822.3267	822.3780	-62.3577	461	467	(R)(M)
868.6112	868.4933	135.7888	481	487	(K)(T)
1361.7964	1361.6120	135.4884	667	680	(R)(L)
1377.8012	1377.6069	141.0417	667	680	(R)GPVSGSPDSMNASR(L)	1Met-ox
1383.7995	1383.8364	-26.6344	160	171	(R)(E)
1566.8856	1566.6818	130.0712	580	592	(R)DQRTEGDSQEMVR(L)	1Met-ox
1709.9491	1709.9491	-0.0368	428	441	(R)(E)
1935.1461	1934.8952	129.6699	516	531	(R)EMEQAVELCGRENEVK(L)	1Cys-am
1951.1330	1950.8901	124.4939	516	531	(R)EMEQAVELCGRENEVK(L)	1Met-ox 1Cys-am
2144.3366	2144.1572	83.7097	532	549	(K)(S)
2208.2173	2208.0647	69.1232	376	394	(K)(I)

The matched peptides cover 16% (126/756AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 30.0 Mean Error = 72.3203 ppm Data Tolerance = 147.2123 ppm Mean Missed Cleavages = 0.7