MS-Fit Search Results

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Sample ID (comment): HSG076
Database searched: SwissProt.8.20.2001
Molecular weight search (1000 - 100000 Da) selects 94194 entries.
Full pI range: 99754 entries.
Species search ( HUMAN MOUSE ) selects 11713 entries.
Combined molecular weight, pI and species searches select 10428 entries.
Pre searches select 10428 entries.
MS-Fit search selects 246 entries (results displayed for top 5 matches).

Parameters Used in Search

Considered modifications: | Oxidation of M | Protein N-terminus Acetylated | Acrylamide Modified Cys |

4	75.000 ppm	monoisotopic	Trypsin	3	carbamidomethylation	Hydrogen (H)	Free Acid (O H)
Min. # Peptides to Match	Peptide Mass Tolerance (+/-)	Peptide Masses are	Digest Used	Max. # Missed Cleavages	Cysteines Modified by	Peptide N terminus	Peptide C terminus

Result Summary

Rank	MOWSE Score	# (%) Masses Matched	Protein MW (Da)/pI	Species	Protein Name
1	3.4e+007	15/37 (40%)	41737.1 / 5.29	HUMAN	ACTIN, CYTOPLASMIC 1 (BETA-ACTIN)
2	3.4e+007	15/37 (40%)	41793.2 / 5.31	HUMAN	ACTIN, CYTOPLASMIC 2 (GAMMA-ACTIN)
3	1.22e+005	10/37 (27%)	42019.4 / 5.23	HUMAN	ACTIN, ALPHA CARDIAC
4	1.22e+005	10/37 (27%)	42051.4 / 5.23	HUMAN	ACTIN, ALPHA SKELETAL MUSCLE (ALPHA-ACTIN 1)
5	3.5e+004	9/37 (24%)	41877.3 / 5.31	HUMAN	ACTIN, GAMMA-ENTERIC SMOOTH MUSCLE (ALPHA-ACTIN 3)

Detailed Results

1. 15/37 matches (40%). 41737.1 Da, pI = 5.29. Acc. # P02570. HUMAN. ACTIN, CYTOPLASMIC 1 (BETA-ACTIN).

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
791.3162	791.3722	-70.6797	285	290	(K)CDVDIR(K)	1Cys-am
795.4473	795.4729	-32.1712	329	335	(K)(K)
976.4157	976.4488	-33.9602	19	28	(K)(A)
1132.4831	1132.5275	-39.1669	197	206	(R)(E)
1171.5219	1171.5716	-42.4676	40	50	(R)(D)
1187.5358	1187.5665	-25.8507	40	50	(R)HQGVMVGMGQK(D)	1Met-ox
1198.6561	1198.7061	-41.6392	29	39	(R)(H)
1515.7072	1515.7497	-28.0362	85	95	(K)(V)
1516.6954	1516.7032	-5.1679	360	372	(K)(K)
1790.8213	1790.8925	-39.7235	239	254	(K)(F)
1953.9992	1954.0650	-33.6670	96	113	(R)(A)
2215.0291	2215.0705	-18.6970	292	312	(K)(M)
2231.0260	2231.0654	-17.6498	292	312	(K)DLYANTVLSGGTTMYPGIADR(M)	1Met-ox
2564.1787	2564.1900	-4.4314	216	238	(K)LCYVALDFEQEMATAASSSSLEK(S)	1Cys-am
3183.5094	3183.6149	-33.1216	148	177	(R)(L)

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Click individual mass to do a non-specific cleavage search.

The matched peptides cover 49% (187/375AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 40.5 Mean Error = -31.0953 ppm Data Tolerance = 32.6088 ppm Mean Missed Cleavages = 0.0

2. 15/37 matches (40%). 41793.2 Da, pI = 5.31. Acc. # P02571. HUMAN. ACTIN, CYTOPLASMIC 2 (GAMMA-ACTIN).

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
791.3162 791.3722 -70.6797 285 290 (K)CDVDIR(K) 1Cys-am
795.4473 795.4729 -32.1712 329 335 (K)(K)
976.4157 976.4488 -33.9602 19 28 (K)(A)
1132.4831 1132.5275 -39.1669 197 206 (R)(E)
1171.5219 1171.5716 -42.4676 40 50 (R)(D)
1187.5358 1187.5665 -25.8507 40 50 (R)HQGVMVGMGQK(D) 1Met-ox
1198.6561 1198.7061 -41.6392 29 39 (R)(H)
1515.7072 1515.7497 -28.0362 85 95 (K)(V)
1516.6954 1516.7032 -5.1679 360 372 (K)(K)
1790.8213 1790.8925 -39.7235 239 254 (K)(F)
1953.9992 1954.0650 -33.6670 96 113 (R)(A)
2215.0291 2215.0705 -18.6970 292 312 (K)(M)
2231.0260 2231.0654 -17.6498 292 312 (K)DLYANTVLSGGTTMYPGIADR(M) 1Met-ox
2564.1787 2564.1900 -4.4314 216 238 (K)LCYVALDFEQEMATAASSSSLEK(S) 1Cys-am
3183.5094 3183.6149 -33.1216 148 177 (R)(L)

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Click individual mass to do a non-specific cleavage search.

The matched peptides cover 49% (187/375AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 40.5 Mean Error = -31.0953 ppm Data Tolerance = 32.6088 ppm Mean Missed Cleavages = 0.0

3. 10/37 matches (27%). 42019.4 Da, pI = 5.23. Acc. # P04270. HUMAN. ACTIN, ALPHA CARDIAC.

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
791.3162 791.3722 -70.6797 287 292 (K)(K)
795.4473 795.4729 -32.1712 331 337 (K)(K)
976.4157 976.4488 -33.9602 21 30 (K)(A)
1171.5219 1171.5716 -42.4676 42 52 (R)(D)
1187.5358 1187.5665 -25.8507 42 52 (R)HQGVMVGMGQK(D) 1Met-ox
1198.6561 1198.7061 -41.6392 31 41 (R)(H)
1515.7072 1515.7497 -28.0362 87 97 (K)(V)
1790.8213 1790.8925 -39.7235 241 256 (K)(F)
1960.8704 1960.9115 -20.9333 71 86 (K)(I)
1976.9346 1976.9064 14.2404 71 86 (K)YPIEHGIITNWDDMEK(I) 1Met-ox

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Click individual mass to do a non-specific cleavage search.

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
791.3162	791.3722	-70.6797	287	292	(K)(K)
795.4473	795.4729	-32.1712	331	337	(K)(K)
976.4157	976.4488	-33.9602	21	30	(K)(A)
1171.5219	1171.5716	-42.4676	42	52	(R)(D)
1187.5358	1187.5665	-25.8507	42	52	(R)HQGVMVGMGQK(D)	1Met-ox
1198.6561	1198.7061	-41.6392	31	41	(R)(H)
1515.7072	1515.7497	-28.0362	87	97	(K)(V)
1790.8213	1790.8925	-39.7235	241	256	(K)(F)
1960.8704	1960.9115	-20.9333	71	86	(K)(I)
1976.9346	1976.9064	14.2404	71	86	(K)YPIEHGIITNWDDMEK(I)	1Met-ox

The matched peptides cover 23% (88/377AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 27.0 Mean Error = -32.1221 ppm Data Tolerance = 42.5632 ppm Mean Missed Cleavages = 0.0

4. 10/37 matches (27%). 42051.4 Da, pI = 5.23. Acc. # P02568. HUMAN. ACTIN, ALPHA SKELETAL MUSCLE (ALPHA-ACTIN 1).

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
791.3162 791.3722 -70.6797 287 292 (K)(K)
795.4473 795.4729 -32.1712 331 337 (K)(K)
976.4157 976.4488 -33.9602 21 30 (K)(A)
1171.5219 1171.5716 -42.4676 42 52 (R)(D)
1187.5358 1187.5665 -25.8507 42 52 (R)HQGVMVGMGQK(D) 1Met-ox
1198.6561 1198.7061 -41.6392 31 41 (R)(H)
1515.7072 1515.7497 -28.0362 87 97 (K)(V)
1790.8213 1790.8925 -39.7235 241 256 (K)(F)
1960.8704 1960.9115 -20.9333 71 86 (K)(I)
1976.9346 1976.9064 14.2404 71 86 (K)YPIEHGIITNWDDMEK(I) 1Met-ox

Click link below to search for another component.

Click individual mass to do a non-specific cleavage search.

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
791.3162	791.3722	-70.6797	287	292	(K)(K)
795.4473	795.4729	-32.1712	331	337	(K)(K)
976.4157	976.4488	-33.9602	21	30	(K)(A)
1171.5219	1171.5716	-42.4676	42	52	(R)(D)
1187.5358	1187.5665	-25.8507	42	52	(R)HQGVMVGMGQK(D)	1Met-ox
1198.6561	1198.7061	-41.6392	31	41	(R)(H)
1515.7072	1515.7497	-28.0362	87	97	(K)(V)
1790.8213	1790.8925	-39.7235	241	256	(K)(F)
1960.8704	1960.9115	-20.9333	71	86	(K)(I)
1976.9346	1976.9064	14.2404	71	86	(K)YPIEHGIITNWDDMEK(I)	1Met-ox

The matched peptides cover 23% (88/377AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 27.0 Mean Error = -32.1221 ppm Data Tolerance = 42.5632 ppm Mean Missed Cleavages = 0.0

5. 9/37 matches (24%). 41877.3 Da, pI = 5.31. Acc. # P12718. HUMAN. ACTIN, GAMMA-ENTERIC SMOOTH MUSCLE (ALPHA-ACTIN 3).

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
791.3162 791.3722 -70.6797 286 291 (K)(K)
795.4473 795.4729 -32.1712 330 336 (K)(K)
976.4157 976.4488 -33.9602 20 29 (K)(A)
1171.5219 1171.5716 -42.4676 41 51 (R)(D)
1187.5358 1187.5665 -25.8507 41 51 (R)HQGVMVGMGQK(D) 1Met-ox
1198.6561 1198.7061 -41.6392 30 40 (R)(H)
1790.8213 1790.8925 -39.7235 240 255 (K)(F)
1960.8704 1960.9115 -20.9333 70 85 (K)(I)
1976.9346 1976.9064 14.2404 70 85 (K)YPIEHGIITNWDDMEK(I) 1Met-ox

Click link below to search for another component.

Click individual mass to do a non-specific cleavage search.

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
791.3162	791.3722	-70.6797	286	291	(K)(K)
795.4473	795.4729	-32.1712	330	336	(K)(K)
976.4157	976.4488	-33.9602	20	29	(K)(A)
1171.5219	1171.5716	-42.4676	41	51	(R)(D)
1187.5358	1187.5665	-25.8507	41	51	(R)HQGVMVGMGQK(D)	1Met-ox
1198.6561	1198.7061	-41.6392	30	40	(R)(H)
1790.8213	1790.8925	-39.7235	240	255	(K)(F)
1960.8704	1960.9115	-20.9333	70	85	(K)(I)
1976.9346	1976.9064	14.2404	70	85	(K)YPIEHGIITNWDDMEK(I)	1Met-ox

The matched peptides cover 20% (77/376AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 24.3 Mean Error = -32.5761 ppm Data Tolerance = 45.0422 ppm Mean Missed Cleavages = 0.0