MS-Fit Search Results

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Sample ID (comment): HSG077
Database searched: SwissProt.12.15.2000
Molecular weight search (1000 - 100000 Da) selects 86001 entries.
Full pI range: 90939 entries.
Species search ( HUMAN MOUSE ) selects 10164 entries.
Combined molecular weight, pI and species searches select 9152 entries.
Pre searches select 9152 entries.
MS-Fit search selects 318 entries (results displayed for top 5 matches).

Parameters Used in Search

Considered modifications: | Oxidation of M | Protein N-terminus Acetylated |

4	60.000 ppm	monoisotopic	Trypsin	3	acrylamide	Hydrogen (H)	Free Acid (O H)
Min. # Peptides to Match	Peptide Mass Tolerance (+/-)	Peptide Masses are	Digest Used	Max. # Missed Cleavages	Cysteines Modified by	Peptide N terminus	Peptide C terminus

Result Summary

Rank	MOWSE Score	# (%) Masses Matched	Protein MW (Da)/pI	Species	Protein Name
1	2.83e+005	15/31 (48%)	59671.6 / 5.45	HUMAN	T-COMPLEX PROTEIN 1, EPSILON SUBUNIT (TCP-1-EPSILON) (CCT-EPSILON) (KIAA0098)
2	4.33e+004	10/31 (32%)	59624.6 / 5.72	MOUSE	T-COMPLEX PROTEIN 1, EPSILON SUBUNIT (TCP-1-EPSILON) (CCT-EPSILON)
3	5.68e+003	9/31 (29%)	57878.3 / 7.58	HUMAN	PYRUVATE KINASE, M1 ISOZYME (PYRUVATE KINASE MUSCLE ISOZYME) (CYTOSOLIC THYROID HORMONE-BINDING PROTEIN) (CTHBP) (THBP1)
4	5.68e+003	9/31 (29%)	57914.4 / 7.95	HUMAN	PYRUVATE KINASE, M2 ISOZYME
5	145	4/31 (12%)	23373.2 / 10.22	HUMAN	TRANSCRIPTION FACTOR BTF3 HOMOLOG 3

Detailed Results

1. 15/31 matches (48%). 59671.6 Da, pI = 5.45. Acc. # P48643. HUMAN. T-COMPLEX PROTEIN 1, EPSILON SUBUNIT (TCP-1-EPSILON) (CCT-EPSILON) (KIAA0098).

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
819.5138	819.4650	59.5660	90	96	(K)(S)
819.5138	819.5092	5.5694	382	388	(R)(G)
1093.5618	1093.5278	31.0527	133	142	(R)(V)
1197.6611	1197.6414	16.4240	401	410	(R)(N)
1274.7257	1274.6891	28.7510	515	525	(K)(M)
1282.6473	1282.5924	42.7846	440	449	(K)(A)
1624.9514	1624.9137	23.2390	248	261	(K)(T)
1667.9348	1667.9008	20.3385	353	368	(K)(D)
1713.9058	1713.8634	24.7482	227	241	(K)GVIVDKDFSHPQMPK(K)	1Met-ox
1738.9847	1738.9492	20.4149	324	340	(R)(I)
1961.0809	1960.9836	49.6177	184	201	(R)(R)
1977.0237	1976.9785	22.8601	184	201	(R)QMAEIAVNAVLTVADMER(R)	1Met-ox
2171.2272	2171.1211	48.8816	1	20	(-)ASMGTLAFDEYGRPFLIIK(D)	Acet N
2185.2868	2185.1968	41.2290	151	170	(K)(T)
2187.2264	2187.1160	50.4702	1	20	(-)ASMGTLAFDEYGRPFLIIK(D)	1Met-ox Acet N
3393.8552	3393.6650	56.0363	294	323	(K)(W)

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The matched peptides cover 37% (205/541AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 48.4 Mean Error = 33.8740 ppm Data Tolerance = 32.0631 ppm Mean Missed Cleavages = 0.1

2. 10/31 matches (32%). 59624.6 Da, pI = 5.72. Acc. # P80316. MOUSE. T-COMPLEX PROTEIN 1, EPSILON SUBUNIT (TCP-1-EPSILON) (CCT-EPSILON).

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
819.5138 819.4650 59.5660 90 96 (K)(S)
819.5138 819.5092 5.5694 382 388 (R)(G)
1093.5618 1093.5278 31.0527 133 142 (R)(I)
1197.6611 1197.6414 16.4240 401 410 (R)(N)
1274.7257 1274.6891 28.7510 515 525 (K)(M)
1282.6473 1282.5924 42.7846 440 449 (K)(A)
1624.9514 1624.9137 23.2390 248 261 (K)(T)
1713.9058 1713.8634 24.7482 227 241 (K)GVIVDKDFSHPQMPK(K) 1Met-ox
1738.9847 1738.9492 20.4149 324 340 (R)(I)
1961.0809 1960.9836 49.6177 184 201 (R)(R)
1977.0237 1976.9785 22.8601 184 201 (R)QMAEIAVNAVLTVADMER(R) 1Met-ox

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The matched peptides cover 21% (119/541AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 32.3 Mean Error = 29.5480 ppm Data Tolerance = 31.1138 ppm Mean Missed Cleavages = 0.1

3. 9/31 matches (29%). 57878.3 Da, pI = 7.58. Acc. # P14618. HUMAN. PYRUVATE KINASE, M1 ISOZYME (PYRUVATE KINASE MUSCLE ISOZYME) (CYTOSOLIC THYROID HORMONE-BINDING PROTEIN) (CTHBP) (THBP1).

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
1019.5354 1019.5162 18.8818 368 376 (K)(M)
1197.6611 1197.6479 10.9756 33 43 (R)(N)
1642.8623 1642.7713 55.3883 476 489 (K)(V)
1667.9348 1667.8678 40.1335 137 151 (K)(C)
1837.9819 1837.9118 38.1233 279 294 (R)RFDEILEASDGIMVAR(G) 1Met-ox
1859.9721 1859.9002 38.6508 231 246 (K)(K)
1932.0357 1931.9867 25.3758 384 399 (R)(R)
2088.1425 2088.0878 26.1722 384 400 (R)(L)
2465.4108 2465.2928 47.8547 93 115 (R)(G)

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m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
1019.5354	1019.5162	18.8818	368	376	(K)(M)
1197.6611	1197.6479	10.9756	33	43	(R)(N)
1642.8623	1642.7713	55.3883	476	489	(K)(V)
1667.9348	1667.8678	40.1335	137	151	(K)(C)
1837.9819	1837.9118	38.1233	279	294	(R)RFDEILEASDGIMVAR(G)	1Met-ox
1859.9721	1859.9002	38.6508	231	246	(K)(K)
1932.0357	1931.9867	25.3758	384	399	(R)(R)
2088.1425	2088.0878	26.1722	384	400	(R)(L)
2465.4108	2465.2928	47.8547	93	115	(R)(G)

The matched peptides cover 22% (121/531AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 29.0 Mean Error = 33.5062 ppm Data Tolerance = 28.4412 ppm Mean Missed Cleavages = 0.3

4. 9/31 matches (29%). 57914.4 Da, pI = 7.95. Acc. # P14786. HUMAN. PYRUVATE KINASE, M2 ISOZYME.

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
1019.5354 1019.5162 18.8818 368 376 (K)(M)
1197.6611 1197.6479 10.9756 33 43 (R)(N)
1642.8623 1642.7713 55.3883 476 489 (K)(V)
1667.9348 1667.8678 40.1335 137 151 (K)(C)
1837.9819 1837.9118 38.1233 279 294 (R)RFDEILEASDGIMVAR(G) 1Met-ox
1859.9721 1859.9002 38.6508 231 246 (K)(K)
1932.0357 1931.9867 25.3758 384 399 (R)(R)
2088.1425 2088.0878 26.1722 384 400 (R)(L)
2465.4108 2465.2928 47.8547 93 115 (R)(G)

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Click individual mass to do a non-specific cleavage search.

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
1019.5354	1019.5162	18.8818	368	376	(K)(M)
1197.6611	1197.6479	10.9756	33	43	(R)(N)
1642.8623	1642.7713	55.3883	476	489	(K)(V)
1667.9348	1667.8678	40.1335	137	151	(K)(C)
1837.9819	1837.9118	38.1233	279	294	(R)RFDEILEASDGIMVAR(G)	1Met-ox
1859.9721	1859.9002	38.6508	231	246	(K)(K)
1932.0357	1931.9867	25.3758	384	399	(R)(R)
2088.1425	2088.0878	26.1722	384	400	(R)(L)
2465.4108	2465.2928	47.8547	93	115	(R)(G)

The matched peptides cover 22% (121/531AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 29.0 Mean Error = 33.5062 ppm Data Tolerance = 28.4412 ppm Mean Missed Cleavages = 0.3

5. 4/31 matches (12%). 23373.2 Da, pI = 10.22. Acc. # Q13892. HUMAN. TRANSCRIPTION FACTOR BTF3 HOMOLOG 3.

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
1837.9819 1837.8939 47.8857 15 32 (R)(S)
1961.0809 1960.9703 56.3835 92 108 (K)(F)
1977.0237 1976.9653 29.5709 92 108 (K)VNMFTNQGTVIHFNNPK(F) 1Met-ox
2187.2264 2187.2865 -27.4893 73 91 (K)(V)

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m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
1837.9819	1837.8939	47.8857	15	32	(R)(S)
1961.0809	1960.9703	56.3835	92	108	(K)(F)
1977.0237	1976.9653	29.5709	92	108	(K)VNMFTNQGTVIHFNNPK(F)	1Met-ox
2187.2264	2187.2865	-27.4893	73	91	(K)(V)

The matched peptides cover 25% (54/214AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 12.9 Mean Error = 26.5877 ppm Data Tolerance = 75.4950 ppm Mean Missed Cleavages = 1.0