MS-Fit Search Results

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Sample ID (comment): HSG078
Database searched: SwissProt.12.15.2000
Molecular weight search (1000 - 100000 Da) selects 86001 entries.
Full pI range: 90939 entries.
Species search ( HUMAN MOUSE ) selects 10164 entries.
Combined molecular weight, pI and species searches select 9152 entries.
Pre searches select 9152 entries.
MS-Fit search selects 359 entries (results displayed for top 5 matches).

Parameters Used in Search

Considered modifications: | Oxidation of M | Protein N-terminus Acetylated |

4	75.000 ppm	monoisotopic	Trypsin	3	acrylamide	Hydrogen (H)	Free Acid (O H)
Min. # Peptides to Match	Peptide Mass Tolerance (+/-)	Peptide Masses are	Digest Used	Max. # Missed Cleavages	Cysteines Modified by	Peptide N terminus	Peptide C terminus

Result Summary

Rank	MOWSE Score	# (%) Masses Matched	Protein MW (Da)/pI	Species	Protein Name
1	1.2e+005	11/33 (33%)	50976.7 / 5.39	HUMAN	HETEROGENEOUS NUCLEAR RIBONUCLEOPROTEIN K (HNRNP K) (DC-STRETCH BINDING PROTEIN) (CSBP) (TRANSFORMATION UPREGULATED NUCLEAR PROTEIN) (TUNP)
2	550	6/33 (18%)	66018.2 / 8.16	HUMAN	KERATIN, TYPE II CYTOSKELETAL 1 (CYTOKERATIN 1) (K1) (CK 1) (67 KDA CYTOKERATIN) (HAIR ALPHA PROTEIN)
3	107	5/33 (15%)	85253.2 / 6.82	HUMAN	PROBABLE SERINE/THREONINE PROTEIN KINASE SNF1LK
4	96	5/33 (15%)	75492.4 / 5.97	HUMAN	COAGULATION FACTOR XIII B CHAIN PRECURSOR (PROTEIN-GLUTAMINE GAMMA-GLUTAMYLTRANSFERASE B CHAIN) (TRANSGLUTAMINASE B CHAIN)
5	72.1	5/33 (15%)	59556.0 / 5.44	MOUSE	T-COMPLEX PROTEIN 1, THETA SUBUNIT (TCP-1-THETA) (CCT-THETA)

Detailed Results

1. 11/33 matches (33%). 50976.7 Da, pI = 5.39. Acc. # Q07244. HUMAN. HETEROGENEOUS NUCLEAR RIBONUCLEOPROTEIN K (HNRNP K) (DC-STRETCH BINDING PROTEIN) (CSBP) (TRANSFORMATION UPREGULATED NUCLEAR PROTEIN) (TUNP).

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
1112.5044	1112.4682	32.5185	140	148	(K)(L)
1194.7227	1194.6999	19.1085	306	316	(R)(G)
1259.6109	1259.5756	28.0269	423	433	(K)(I)
1518.9661	1518.9372	19.0151	149	163	(R)(G)
1577.7604	1577.6841	48.4099	180	191	(K)(V)
1735.8317	1735.8074	13.9954	22	35	(K)(S)
1751.8702	1751.8023	38.7908	22	35	(K)RPAEDMEEEQAFKR(S)	1Met-ox
1780.8672	1780.7990	38.2980	70	86	(R)(I)
1843.1280	1843.0792	26.4489	87	103	(R)(I)
1917.0908	1917.0333	29.9635	378	396	(R)(D)
2589.5202	2589.3888	50.7248	434	456	(R)(Q)

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The matched peptides cover 31% (148/463AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 33.3 Mean Error = 31.3909 ppm Data Tolerance = 23.7620 ppm Mean Missed Cleavages = 0.3

2. 6/33 matches (18%). 66018.2 Da, pI = 8.16. Acc. # P04264. HUMAN. KERATIN, TYPE II CYTOSKELETAL 1 (CYTOKERATIN 1) (K1) (CK 1) (67 KDA CYTOKERATIN) (HAIR ALPHA PROTEIN).

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
832.5057 832.4892 19.8133 75 82 (K)(G)
1179.6352 1179.6010 29.0455 377 386 (K)(H)
1475.8412 1475.7858 37.5294 200 211 (R)(W)
1475.8412 1475.7494 62.1860 212 223 (K)(T)
1751.8702 1751.8272 24.5370 603 624 (R)(S)
1994.0799 1993.9772 51.4985 224 239 (R)(R)
2384.1104 2383.9524 66.2878 519 549 (R)(G)

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m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)
832.5057	832.4892	19.8133	75	82	(K)(G)
1179.6352	1179.6010	29.0455	377	386	(K)(H)
1475.8412	1475.7858	37.5294	200	211	(R)(W)
1475.8412	1475.7494	62.1860	212	223	(K)(T)
1751.8702	1751.8272	24.5370	603	624	(R)(S)
1994.0799	1993.9772	51.4985	224	239	(R)(R)
2384.1104	2383.9524	66.2878	519	549	(R)(G)

The matched peptides cover 17% (111/644AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 18.2 Mean Error = 41.5568 ppm Data Tolerance = 37.1594 ppm Mean Missed Cleavages = 0.1

3. 5/33 matches (15%). 85253.2 Da, pI = 6.82. Acc. # P57059. HUMAN. PROBABLE SERINE/THREONINE PROTEIN KINASE SNF1LK.

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
1179.6352 1179.6560 -17.5902 71 79 (K)(L)
1475.8412 1475.7416 67.5142 152 164 (K)(L)
1715.9424 1715.8604 47.7608 649 662 (R)(L)
2475.1370 2475.2124 -30.4629 1 23 (-)(V)
2717.2668 2717.3887 -44.8473 128 148 (R)(D)

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m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)
1179.6352	1179.6560	-17.5902	71	79	(K)(L)
1475.8412	1475.7416	67.5142	152	164	(K)(L)
1715.9424	1715.8604	47.7608	649	662	(R)(L)
2475.1370	2475.2124	-30.4629	1	23	(-)(V)
2717.2668	2717.3887	-44.8473	128	148	(R)(D)

The matched peptides cover 10% (80/786AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 15.2 Mean Error = 4.4749 ppm Data Tolerance = 99.9391 ppm Mean Missed Cleavages = 0.6

4. 5/33 matches (15%). 75492.4 Da, pI = 5.97. Acc. # P05160. HUMAN. COAGULATION FACTOR XIII B CHAIN PRECURSOR (PROTEIN-GLUTAMINE GAMMA-GLUTAMYLTRANSFERASE B CHAIN) (TRANSGLUTAMINASE B CHAIN).

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
1196.6501 1196.5992 42.5447 35 43 (R)(S)
1838.9801 1838.9322 26.0223 90 105 (K)(L)
1851.0073 1850.9111 51.9763 54 69 (K)(Q)
2097.0783 2097.0075 33.7767 334 352 (K)(I)
2369.1487 2369.0720 32.3723 149 168 (K)(T)

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m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)
1196.6501	1196.5992	42.5447	35	43	(R)(S)
1838.9801	1838.9322	26.0223	90	105	(K)(L)
1851.0073	1850.9111	51.9763	54	69	(K)(Q)
2097.0783	2097.0075	33.7767	334	352	(K)(I)
2369.1487	2369.0720	32.3723	149	168	(K)(T)

The matched peptides cover 12% (80/661AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 15.2 Mean Error = 37.3385 ppm Data Tolerance = 20.1687 ppm Mean Missed Cleavages = 0.4

5. 5/33 matches (15%). 59556.0 Da, pI = 5.44. Acc. # P42932. MOUSE. T-COMPLEX PROTEIN 1, THETA SUBUNIT (TCP-1-THETA) (CCT-THETA).

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
1307.7192 1307.6384 61.7533 21 31 (K)(N)
1780.8672 1780.9519 -47.5783 255 270 (K)(G)
1838.9801 1838.9574 12.3445 121 137 (R)(K)
2384.1104 2384.1114 -0.4047 261 281 (K)(A)
2465.3930 2465.2393 62.3405 38 59 (K)(L)

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m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)
1307.7192	1307.6384	61.7533	21	31	(K)(N)
1780.8672	1780.9519	-47.5783	255	270	(K)(G)
1838.9801	1838.9574	12.3445	121	137	(R)(K)
2384.1104	2384.1114	-0.4047	261	281	(K)(A)
2465.3930	2465.2393	62.3405	38	59	(K)(L)

The matched peptides cover 14% (77/548AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 15.2 Mean Error = 17.6911 ppm Data Tolerance = 92.4726 ppm Mean Missed Cleavages = 0.8