MS-Fit Search Results

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Sample ID (comment): HSG080
Database searched: SwissProt.8.20.2001
Molecular weight search (1000 - 100000 Da) selects 94194 entries.
Full pI range: 99754 entries.
Species search ( HUMAN MOUSE ) selects 11713 entries.
Combined molecular weight, pI and species searches select 10428 entries.
Pre searches select 10428 entries.
MS-Fit search selects 804 entries (results displayed for top 5 matches).

Parameters Used in Search

Considered modifications: | Oxidation of M | Protein N-terminus Acetylated | Acrylamide Modified Cys |

4	50.000 ppm	monoisotopic	Trypsin	3	carbamidomethylation	Hydrogen (H)	Free Acid (O H)
Min. # Peptides to Match	Peptide Mass Tolerance (+/-)	Peptide Masses are	Digest Used	Max. # Missed Cleavages	Cysteines Modified by	Peptide N terminus	Peptide C terminus

Result Summary

Rank	MOWSE Score	# (%) Masses Matched	Protein MW (Da)/pI	Species	Protein Name
1	2.44e+007	17/52 (32%)	34273.8 / 5.72	HUMAN	60S ACIDIC RIBOSOMAL PROTEIN P0 (L10E)
2	1.01e+006	15/52 (28%)	34216.7 / 5.91	MOUSE	60S ACIDIC RIBOSOMAL PROTEIN P0 (L10E)
3	6.52e+004	11/52 (21%)	66018.2 / 8.16	HUMAN	KERATIN, TYPE II CYTOSKELETAL 1 (CYTOKERATIN 1) (K1) (CK 1) (67 KDA CYTOKERATIN) (HAIR ALPHA PROTEIN)
4	924	8/52 (15%)	61987.8 / 5.14	HUMAN	KERATIN, TYPE I CYTOSKELETAL 9 (CYTOKERATIN 9) (K9) (CK 9)
5	240	5/52 (9%)	77241.4 / 6.60	HUMAN	ZINC FINGER PROTEIN 263 (ZINC FINGER PROTEIN FPM315)

Detailed Results

1. 17/52 matches (32%). 34273.8 Da, pI = 5.72. Acc. # P05388. HUMAN. 60S ACIDIC RIBOSOMAL PROTEIN P0 (L10E).

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
720.4182	720.4044	19.1040	215	220	(R)(N)
748.4932	748.4834	13.0913	78	83	(K)(G)
771.4194	771.4041	19.7963	11	16	(K)(I)
803.4345	803.4198	18.3219	39	44	(K)(M)
875.4492	875.4474	1.9723	93	99	(K)(D)
968.5404	968.5205	20.5388	84	92	(R)(E)
1217.6978	1217.6782	16.1088	17	26	(K)(C)
1221.6518	1221.6228	23.7332	67	77	(R)(L)
1280.6351	1280.6309	3.2891	27	38	(K)CFIVGADNVGSK(Q)	1Cys-am
1313.7272	1313.7105	12.6943	135	146	(K)(I)
1428.8526	1428.8314	14.8486	150	162	(R)(T)
1475.7884	1475.7939	-3.7144	39	50	(K)(A)
1676.8533	1676.8529	0.2118	93	106	(K)EDLTEIRDMLLANK(V)	1Met-ox
1824.9323	1824.9496	-9.4641	84	99	(R)(D)
1896.0251	1896.0006	12.9315	248	264	(R)(V)
2194.1529	2194.1178	15.9974	113	134	(R)AGAIAPCEVTVPAQNTGLGPEK(T)	1Cys-am
2752.5319	2752.4674	23.4428	267	297	(K)(V)

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The matched peptides cover 57% (181/317AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 32.7 Mean Error = 11.9355 ppm Data Tolerance = 19.7435 ppm Mean Missed Cleavages = 0.2

2. 15/52 matches (28%). 34216.7 Da, pI = 5.91. Acc. # P14869. MOUSE. 60S ACIDIC RIBOSOMAL PROTEIN P0 (L10E).

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
720.4182 720.4044 19.1040 215 220 (R)(N)
748.4932 748.4834 13.0913 78 83 (K)(G)
771.4194 771.4041 19.7963 11 16 (K)(I)
803.4345 803.4198 18.3219 39 44 (K)(M)
875.4492 875.4474 1.9723 93 99 (K)(D)
968.5404 968.5205 20.5388 84 92 (R)(E)
1217.6978 1217.6782 16.1088 17 26 (K)(C)
1221.6518 1221.6228 23.7332 67 77 (R)(L)
1280.6351 1280.6309 3.2891 27 38 (K)CFIVGADNVGSK(Q) 1Cys-am
1313.7272 1313.7105 12.6943 135 146 (K)(I)
1428.8526 1428.8314 14.8486 150 162 (R)(T)
1475.7884 1475.7939 -3.7144 39 50 (K)(A)
1676.8533 1676.8529 0.2118 93 106 (K)EDLTEIRDMLLANK(V) 1Met-ox
1824.9323 1824.9496 -9.4641 84 99 (R)(D)
2194.1529 2194.1178 15.9974 113 134 (R)AGAIAPCEVTVPAQNTGLGPEK(T) 1Cys-am

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The matched peptides cover 41% (133/317AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 28.8 Mean Error = 11.1020 ppm Data Tolerance = 20.1096 ppm Mean Missed Cleavages = 0.3

3. 11/52 matches (21%). 66018.2 Da, pI = 8.16. Acc. # P04264. HUMAN. KERATIN, TYPE II CYTOSKELETAL 1 (CYTOKERATIN 1) (K1) (CK 1) (67 KDA CYTOKERATIN) (HAIR ALPHA PROTEIN).

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
1033.5046 1033.5166 -11.5853 484 492 (R)(M)
1127.5575 1127.5407 14.9229 289 298 (K)(V)
1179.6283 1179.6010 23.1664 377 386 (K)(H)
1277.7333 1277.7105 17.8308 473 483 (K)(T)
1300.5640 1300.5302 25.9598 258 267 (K)(N)
1302.6494 1302.7018 -40.1639 393 403 (R)(V)
1383.7105 1383.6909 14.1733 186 197 (K)(V)
1475.7884 1475.7858 1.7600 200 211 (R)(W)
1475.7884 1475.7494 26.4157 212 223 (K)(T)
1716.8718 1716.8517 11.7487 418 432 (K)(G)
1994.0259 1993.9772 24.4350 224 239 (R)(R)
2383.9867 2383.9524 14.4009 519 549 (R)(G)

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m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)
1033.5046	1033.5166	-11.5853	484	492	(R)(M)
1127.5575	1127.5407	14.9229	289	298	(K)(V)
1179.6283	1179.6010	23.1664	377	386	(K)(H)
1277.7333	1277.7105	17.8308	473	483	(K)(T)
1300.5640	1300.5302	25.9598	258	267	(K)(N)
1302.6494	1302.7018	-40.1639	393	403	(R)(V)
1383.7105	1383.6909	14.1733	186	197	(K)(V)
1475.7884	1475.7858	1.7600	200	211	(R)(W)
1475.7884	1475.7494	26.4157	212	223	(K)(T)
1716.8718	1716.8517	11.7487	418	432	(K)(G)
1994.0259	1993.9772	24.4350	224	239	(R)(R)
2383.9867	2383.9524	14.4009	519	549	(R)(G)

The matched peptides cover 24% (159/644AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 21.2 Mean Error = 10.2553 ppm Data Tolerance = 38.5216 ppm Mean Missed Cleavages = 0.2

4. 8/52 matches (15%). 61987.8 Da, pI = 5.14. Acc. # P35527. HUMAN. KERATIN, TYPE I CYTOSKELETAL 9 (CYTOKERATIN 9) (K9) (CK 9).

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
1065.4885 1065.4999 -10.6416 154 162 (K)(L)
1095.5988 1095.5475 46.8024 184 191 (K)(G)
1307.6918 1307.6782 10.4151 240 249 (R)(Q)
1707.7854 1707.7509 20.2188 46 62 (R)(G)
1791.7157 1791.7283 -6.9964 490 512 (R)(G)
1837.9630 1837.9660 -1.6060 374 389 (R)(K)
2705.2020 2705.1617 14.9183 63 94 (R)(G)
2752.5319 2752.4014 47.4331 427 448 (R)(E)

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m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)
1065.4885	1065.4999	-10.6416	154	162	(K)(L)
1095.5988	1095.5475	46.8024	184	191	(K)(G)
1307.6918	1307.6782	10.4151	240	249	(R)(Q)
1707.7854	1707.7509	20.2188	46	62	(R)(G)
1791.7157	1791.7283	-6.9964	490	512	(R)(G)
1837.9630	1837.9660	-1.6060	374	389	(R)(K)
2705.2020	2705.1617	14.9183	63	94	(R)(G)
2752.5319	2752.4014	47.4331	427	448	(R)(E)

The matched peptides cover 22% (137/622AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 15.4 Mean Error = 15.0679 ppm Data Tolerance = 44.9038 ppm Mean Missed Cleavages = 0.6

5. 5/52 matches (9%). 77241.4 Da, pI = 6.60. Acc. # O14978. HUMAN. ZINC FINGER PROTEIN 263 (ZINC FINGER PROTEIN FPM315).

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
771.4194 771.3936 33.4705 674 679 (R)(T)
875.4492 875.4375 13.2763 49 56 (R)(E)
1127.5575 1127.5229 30.6732 574 582 (K)(S)
1824.9323 1824.9002 17.6253 62 75 (R)(T)
2752.5319 2752.5501 -6.5908 78 100 (K)(V)

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m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)
771.4194	771.3936	33.4705	674	679	(R)(T)
875.4492	875.4375	13.2763	49	56	(R)(E)
1127.5575	1127.5229	30.6732	574	582	(K)(S)
1824.9323	1824.9002	17.6253	62	75	(R)(T)
2752.5319	2752.5501	-6.5908	78	100	(K)(V)

The matched peptides cover 8% (60/683AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 9.6 Mean Error = 17.6909 ppm Data Tolerance = 32.0410 ppm Mean Missed Cleavages = 0.0