MS-Fit Search Results

Press stop on your browser if you wish to abort this MS-Fit search prematurely.
Sample ID (comment): HSG084
Database searched: SwissProt.1.20.2002
Molecular weight search (1000 - 100000 Da) selects 98142 entries.
Full pI range: 103987 entries.
Species search ( HUMAN MOUSE ) selects 12504 entries.
Combined molecular weight, pI and species searches select 11102 entries.
Pre searches select 11102 entries.
MS-Fit search selects 1385 entries (results displayed for top 5 matches).

Parameters Used in Search

Considered modifications: | Oxidation of M | Protein N-terminus Acetylated | Acrylamide Modified Cys |

4	50.000 ppm	monoisotopic	Trypsin	3	carbamidomethylation	Hydrogen (H)	Free Acid (O H)
Min. # Peptides to Match	Peptide Mass Tolerance (+/-)	Peptide Masses are	Digest Used	Max. # Missed Cleavages	Cysteines Modified by	Peptide N terminus	Peptide C terminus

Result Summary

Rank	MOWSE Score	# (%) Masses Matched	Protein MW (Da)/pI	Species	Protein Name
1	1.72e+005	10/54 (18%)	29804.3 / 5.57	HUMAN	PROHIBITIN
2	4.88e+004	9/54 (16%)	29820.3 / 5.57	MOUSE	PROHIBITIN (B-CELL RECEPTOR ASSOCIATED PROTEIN 32) (BAP 32)
3	1.09e+004	11/54 (20%)	59519.1 / 5.13	HUMAN	KERATIN, TYPE I CYTOSKELETAL 10 (CYTOKERATIN 10) (K10) (CK 10)
4	1.07e+003	9/54 (16%)	66018.2 / 8.16	HUMAN	Keratin, type II cytoskeletal 1 (Cytokeratin 1) (K1) (CK 1) (67 kDa cytokeratin) (Hair alpha protein)
5	403	6/54 (11%)	64466.2 / 6.39	HUMAN	BIFUNCTIONAL PURINE BIOSYNTHESIS PROTEIN PURH [INCLUDES: PHOSPHORIBOSYLAMINOIMIDAZOLECARBOXAMIDE FORMYLTRANSFERASE (AICAR TRANSFORMYLASE); IMP CYCLOHYDROLASE (INOSINICASE) (IMP SYNTHETASE) (ATIC)]

Detailed Results

1. 10/54 matches (18%). 29804.3 Da, pI = 5.57. Acc. # P35232. HUMAN. PROHIBITIN.

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)
720.4138	720.4044	12.9922	36	41	(R)(F)
1058.5316	1058.5231	8.0802	187	195	(K)(A)
1149.6244	1149.5904	29.5416	134	143	(R)(E)
1185.7015	1185.6592	35.7055	84	93	(K)(I)
1396.8834	1396.8429	28.9983	94	105	(R)(I)
1444.7032	1444.6596	30.1718	106	117	(R)(V)
1606.8958	1606.8441	32.1974	240	253	(R)(S)
1998.1360	1998.0871	24.4711	220	239	(K)(K)
2119.2448	2119.1439	47.5979	158	177	(R)(E)
2371.3319	2371.2523	33.5929	12	35	(K)(A)

Click link below to search for another component.

Click individual mass to do a non-specific cleavage search.

The matched peptides cover 50% (137/272AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 18.5 Mean Error = 28.3349 ppm Data Tolerance = 22.4480 ppm Mean Missed Cleavages = 0.1

2. 9/54 matches (16%). 29820.3 Da, pI = 5.57. Acc. # P24142. MOUSE. PROHIBITIN (B-CELL RECEPTOR ASSOCIATED PROTEIN 32) (BAP 32).

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
720.4138 720.4044 12.9922 36 41 (R)(F)
1058.5316 1058.5231 8.0802 187 195 (K)(A)
1149.6244 1149.5904 29.5416 134 143 (R)(E)
1185.7015 1185.6592 35.7055 84 93 (K)(I)
1396.8834 1396.8429 28.9983 94 105 (R)(I)
1606.8958 1606.8441 32.1974 240 253 (R)(S)
1998.1360 1998.0871 24.4711 220 239 (K)(K)
2119.2448 2119.1439 47.5979 158 177 (R)(E)
2371.3319 2371.2523 33.5929 12 35 (K)(A)

Click link below to search for another component.

Click individual mass to do a non-specific cleavage search.

The matched peptides cover 45% (125/272AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 16.7 Mean Error = 28.1308 ppm Data Tolerance = 23.7703 ppm Mean Missed Cleavages = 0.1

3. 11/54 matches (20%). 59519.1 Da, pI = 5.13. Acc. # P13645. HUMAN. KERATIN, TYPE I CYTOSKELETAL 10 (CYTOKERATIN 10) (K10) (CK 10).

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
1090.5526 1090.5315 19.3372 148 156 (K)(L)
1109.4981 1109.4904 6.9601 335 343 (K)(S)
1234.7153 1234.6796 28.9406 236 245 (R)(Q)
1300.6135 1300.5956 13.8006 286 295 (K)(N)
1434.8362 1434.7705 45.8228 440 450 (K)(S)
1444.7032 1444.6855 12.2867 285 295 (K)KNHEEEMKDLR(N) 1Met-ox
1707.8404 1707.7727 39.6659 41 59 (K)(G)
1796.9912 1797.0122 -11.6809 371 386 (R)(Q)
2224.1875 2224.1423 20.3233 267 285 (K)(N)
2367.3245 2367.2632 25.8955 208 228 (K)(L)
2872.4680 2872.3933 25.9899 296 322 (R)(S)

Click link below to search for another component.

Click individual mass to do a non-specific cleavage search.

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
1090.5526	1090.5315	19.3372	148	156	(K)(L)
1109.4981	1109.4904	6.9601	335	343	(K)(S)
1234.7153	1234.6796	28.9406	236	245	(R)(Q)
1300.6135	1300.5956	13.8006	286	295	(K)(N)
1434.8362	1434.7705	45.8228	440	450	(K)(S)
1444.7032	1444.6855	12.2867	285	295	(K)KNHEEEMKDLR(N)	1Met-ox
1707.8404	1707.7727	39.6659	41	59	(K)(G)
1796.9912	1797.0122	-11.6809	371	386	(R)(Q)
2224.1875	2224.1423	20.3233	267	285	(K)(N)
2367.3245	2367.2632	25.8955	208	228	(K)(L)
2872.4680	2872.3933	25.9899	296	322	(R)(S)

The matched peptides cover 25% (151/593AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 20.4 Mean Error = 20.6674 ppm Data Tolerance = 31.4415 ppm Mean Missed Cleavages = 0.5

4. 9/54 matches (16%). 66018.2 Da, pI = 8.16. Acc. # P04264. HUMAN. Keratin, type II cytoskeletal 1 (Cytokeratin 1) (K1) (CK 1) (67 kDa cytokeratin) (Hair alpha protein).

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
1045.5765 1045.5754 0.9757 178 185 (K)(S)
1179.6354 1179.6010 29.1455 377 386 (K)(H)
1277.7563 1277.7105 35.8302 473 483 (K)(T)
1383.7293 1383.6909 27.7898 186 197 (K)(V)
1475.7956 1475.7858 6.6578 200 211 (R)(W)
1475.7956 1475.7494 31.3135 212 223 (K)(T)
1487.7587 1487.8043 -30.6234 240 251 (R)(L)
1638.8954 1638.8604 21.3670 186 199 (K)(F)
1994.0366 1993.9772 29.8097 224 239 (R)(R)
2150.1672 2150.0783 41.3612 224 240 (R)(R)

Click link below to search for another component.

Click individual mass to do a non-specific cleavage search.

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)
1045.5765	1045.5754	0.9757	178	185	(K)(S)
1179.6354	1179.6010	29.1455	377	386	(K)(H)
1277.7563	1277.7105	35.8302	473	483	(K)(T)
1383.7293	1383.6909	27.7898	186	197	(K)(V)
1475.7956	1475.7858	6.6578	200	211	(R)(W)
1475.7956	1475.7494	31.3135	212	223	(K)(T)
1487.7587	1487.8043	-30.6234	240	251	(R)(L)
1638.8954	1638.8604	21.3670	186	199	(K)(F)
1994.0366	1993.9772	29.8097	224	239	(R)(R)
2150.1672	2150.0783	41.3612	224	240	(R)(R)

The matched peptides cover 14% (95/644AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 16.7 Mean Error = 19.3627 ppm Data Tolerance = 43.0809 ppm Mean Missed Cleavages = 0.7

5. 6/54 matches (11%). 64466.2 Da, pI = 6.39. Acc. # P31939. HUMAN. BIFUNCTIONAL PURINE BIOSYNTHESIS PROTEIN PURH [INCLUDES: PHOSPHORIBOSYLAMINOIMIDAZOLECARBOXAMIDE FORMYLTRANSFERASE (AICAR TRANSFORMYLASE); IMP CYCLOHYDROLASE (INOSINICASE) (IMP SYNTHETASE) (ATIC)].

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
1717.9294 1717.8900 22.9521 509 523 (K)(K)
1877.0684 1877.0245 23.3829 389 405 (K)(N)
2119.2448 2119.1624 38.8941 389 407 (K)(D)
2150.1672 2150.1643 1.3450 569 587 (K)VVIEACDELGIILAHTNLR(L) 1Cys-am
2234.1591 2234.1444 6.5772 335 355 (R)(K)
2872.4680 2872.4746 -2.3089 171 194 (R)(Q)

Click link below to search for another component.

Click individual mass to do a non-specific cleavage search.

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
1717.9294	1717.8900	22.9521	509	523	(K)(K)
1877.0684	1877.0245	23.3829	389	405	(K)(N)
2119.2448	2119.1624	38.8941	389	407	(K)(D)
2150.1672	2150.1643	1.3450	569	587	(K)VVIEACDELGIILAHTNLR(L)	1Cys-am
2234.1591	2234.1444	6.5772	335	355	(R)(K)
2872.4680	2872.4746	-2.3089	171	194	(R)(Q)

The matched peptides cover 16% (98/591AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 11.1 Mean Error = 15.1404 ppm Data Tolerance = 31.7656 ppm Mean Missed Cleavages = 1.3