MS-Fit Search Results

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Sample ID (comment): HSG085
Database searched: SwissProt.1.20.2002
Molecular weight search (1000 - 100000 Da) selects 98142 entries.
Full pI range: 103987 entries.
Species search ( HUMAN MOUSE ) selects 12504 entries.
Combined molecular weight, pI and species searches select 11102 entries.
Pre searches select 11102 entries.
MS-Fit search selects 972 entries (results displayed for top 5 matches).

Parameters Used in Search

Considered modifications: | Oxidation of M | Protein N-terminus Acetylated | Acrylamide Modified Cys |

4	50.000 ppm	monoisotopic	Trypsin	3	carbamidomethylation	Hydrogen (H)	Free Acid (O H)
Min. # Peptides to Match	Peptide Mass Tolerance (+/-)	Peptide Masses are	Digest Used	Max. # Missed Cleavages	Cysteines Modified by	Peptide N terminus	Peptide C terminus

Result Summary

Rank	MOWSE Score	# (%) Masses Matched	Protein MW (Da)/pI	Species	Protein Name
1	3.55e+008	24/41 (58%)	56782.9 / 5.99	HUMAN	PROTEIN DISULFIDE ISOMERASE A3 PRECURSOR (DISULFIDE ISOMERASE ER-60) (ERP60) (58 KDA MICROSOMAL PROTEIN) (P58) (ERP57)
2	1.96e+004	16/41 (39%)	56621.8 / 5.99	MOUSE	PROTEIN DISULFIDE ISOMERASE A3 PRECURSOR (DISULFIDE ISOMERASE ER-60) (ERP60) (58 KDA MICROSOMAL PROTEIN) (P58) (ERP57)
3	8.62e+003	15/41 (36%)	77957.3 / 5.79	HUMAN	DELTA-LIKE PROTEIN 1 PRECURSOR (DROSOPHILA DELTA HOMOLOG 1) (DELTA1) (H-DELTA-1)
4	525	8/41 (19%)	62784.2 / 9.32	HUMAN	ZINC FINGER PROTEIN 136
5	310	7/41 (17%)	70438.9 / 9.20	MOUSE	ZINC FINGER PROTEIN 28 (ZFP-28) (MKR5 PROTEIN)

Detailed Results

1. 24/41 matches (58%). 56782.9 Da, pI = 5.99. Acc. # P30101. HUMAN. PROTEIN DISULFIDE ISOMERASE A3 PRECURSOR (DISULFIDE ISOMERASE ER-60) (ERP60) (58 KDA MICROSOMAL PROTEIN) (P58) (ERP57).

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
782.3409	782.3572	-20.8644	141	146	(R)(K)
782.3409	782.3586	-22.5738	275	280	(K)(N)
877.4688	877.4896	-23.6288	297	304	(K)(K)
1084.5481	1084.5679	-18.2145	95	104	(K)(I)
1172.5447	1172.5410	3.1409	336	344	(K)(D)
1188.5357	1188.5359	-0.1614	336	344	(K)FVMQEEFSR(D)	1Met-ox
1191.5959	1191.6010	-4.2421	63	73	(R)(L)
1194.6290	1194.6734	-37.2031	416	425	(K)(D)
1236.5317	1236.5133	14.8950	108	119	(R)(T)
1341.7025	1341.6843	13.5579	449	460	(R)(K)
1347.6705	1347.7021	-23.4658	62	73	(K)(L)
1359.6560	1359.6585	-1.8591	352	362	(R)(R)
1370.7052	1370.6956	7.0143	472	482	(R)(E)
1488.7303	1488.6793	34.2716	336	347	(K)FVMQEEFSRDGK(A)	1Met-ox
1515.7723	1515.7596	8.3821	352	363	(R)(Y)
1619.8150	1619.7845	18.8444	259	271	(K)(N)
1645.8937	1645.8814	7.4208	290	304	(K)(K)
1652.7885	1652.7669	13.0962	105	119	(K)(T)
1664.7759	1664.7590	10.1639	434	448	(K)(G)
1680.7457	1680.7539	-4.9163	434	448	(K)MDATANDVPSPYEVR(G)	1Met-ox
1758.9121	1758.9026	5.4044	131	146	(K)(K)
1887.0218	1886.9976	12.8382	130	146	(K)(K)
1887.0218	1886.9976	12.8382	131	147	(K)(F)
2015.0876	2015.0926	-2.4705	130	147	(K)(F)
2575.3463	2575.3044	16.2643	306	329	(K)(T)
2703.4317	2703.3994	11.9766	305	329	(R)(T)

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The matched peptides cover 36% (186/505AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 58.5 Mean Error = 1.1734 ppm Data Tolerance = 33.3395 ppm Mean Missed Cleavages = 0.6

2. 16/41 matches (39%). 56621.8 Da, pI = 5.99. Acc. # P27773. MOUSE. PROTEIN DISULFIDE ISOMERASE A3 PRECURSOR (DISULFIDE ISOMERASE ER-60) (ERP60) (58 KDA MICROSOMAL PROTEIN) (P58) (ERP57).

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
782.3409 782.3572 -20.8644 140 145 (R)(K)
782.3409 782.3586 -22.5738 274 279 (K)(N)
877.4688 877.4896 -23.6288 296 303 (K)(K)
1084.5481 1084.5679 -18.2145 94 103 (K)(I)
1172.5447 1172.5410 3.1409 335 343 (K)(D)
1188.5357 1188.5359 -0.1614 335 343 (K)FVMQEEFSR(D) 1Met-ox
1191.5959 1191.6010 -4.2421 63 73 (R)(L)
1194.6290 1194.6734 -37.2031 415 424 (K)(D)
1341.7025 1341.6843 13.5579 448 459 (K)(K)
1347.6705 1347.7021 -23.4658 62 73 (K)(L)
1488.7303 1488.6793 34.2716 335 346 (K)FVMQEEFSRDGK(A) 1Met-ox
1645.8937 1645.8814 7.4208 289 303 (K)(K)
1652.7885 1652.7478 24.6504 433 447 (K)MDATANDVPSPYEVK(G) 1Met-ox
1758.9121 1758.9026 5.4044 130 145 (K)(K)
1887.0218 1886.9976 12.8382 129 145 (K)(K)
1887.0218 1886.9976 12.8382 130 146 (K)(F)
2015.0876 2015.0926 -2.4705 129 146 (K)(F)
2575.3463 2575.2932 20.6263 252 273 (K)(G)

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Click individual mass to do a non-specific cleavage search.

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
782.3409	782.3572	-20.8644	140	145	(R)(K)
782.3409	782.3586	-22.5738	274	279	(K)(N)
877.4688	877.4896	-23.6288	296	303	(K)(K)
1084.5481	1084.5679	-18.2145	94	103	(K)(I)
1172.5447	1172.5410	3.1409	335	343	(K)(D)
1188.5357	1188.5359	-0.1614	335	343	(K)FVMQEEFSR(D)	1Met-ox
1191.5959	1191.6010	-4.2421	63	73	(R)(L)
1194.6290	1194.6734	-37.2031	415	424	(K)(D)
1341.7025	1341.6843	13.5579	448	459	(K)(K)
1347.6705	1347.7021	-23.4658	62	73	(K)(L)
1488.7303	1488.6793	34.2716	335	346	(K)FVMQEEFSRDGK(A)	1Met-ox
1645.8937	1645.8814	7.4208	289	303	(K)(K)
1652.7885	1652.7478	24.6504	433	447	(K)MDATANDVPSPYEVK(G)	1Met-ox
1758.9121	1758.9026	5.4044	130	145	(K)(K)
1887.0218	1886.9976	12.8382	129	145	(K)(K)
1887.0218	1886.9976	12.8382	130	146	(K)(F)
2015.0876	2015.0926	-2.4705	129	146	(K)(F)
2575.3463	2575.2932	20.6263	252	273	(K)(G)

The matched peptides cover 26% (132/504AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 39.0 Mean Error = -1.0042 ppm Data Tolerance = 39.3802 ppm Mean Missed Cleavages = 0.8

3. 15/41 matches (36%). 77957.3 Da, pI = 5.79. Acc. # O00548. HUMAN. DELTA-LIKE PROTEIN 1 PRECURSOR (DROSOPHILA DELTA HOMOLOG 1) (DELTA1) (H-DELTA-1).

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
877.4688 877.4783 -10.8272 29 35 (K)(K)
1084.5481 1084.5977 -45.7453 58 65 (R)TFFRVCLK(H) 1Cys-am
1108.4803 1108.5057 -22.9032 691 700 (K)RPDSGCSTSK(D) 1Cys-am
1188.5357 1188.5836 -40.2250 208 217 (R)GEKVCNPGWK(G) 1Cys-am
1236.5317 1236.5254 5.1079 500 509 (R)GHGYVCECAR(G) 2Cys-am
1347.6705 1347.6738 -2.4624 36 46 (K)(G)
1369.6851 1369.6244 44.3215 420 430 (K)CVDLGDAYLCR(C) 2Cys-am
1488.7303 1488.7858 -37.2463 573 584 (R)LQKHRPPADPCR(G) 1Cys-am
1651.7795 1651.7168 37.9707 585 598 (R)GETETMNNLANCQR(E) 1Cys-am
1664.7759 1664.7790 -1.8284 47 61 (R)GGAGPPPCACRTFFR(V) 1Cys-am
1872.9241 1872.8741 26.6707 619 635 (K)(Y)
2211.1465 2211.1562 -4.4032 630 648 (K)(G)
2233.1048 2232.9953 49.0599 420 438 (K)CVDLGDAYLCRCQAGFSGR(H) 2Cys-am
2300.1449 2300.0381 46.4313 37 57 (K)(T)
2752.3578 2752.2466 40.3886 576 598 (K)HRPPADPCRGETETMNNLANCQR(E) 2Cys-am

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m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
877.4688	877.4783	-10.8272	29	35	(K)(K)
1084.5481	1084.5977	-45.7453	58	65	(R)TFFRVCLK(H)	1Cys-am
1108.4803	1108.5057	-22.9032	691	700	(K)RPDSGCSTSK(D)	1Cys-am
1188.5357	1188.5836	-40.2250	208	217	(R)GEKVCNPGWK(G)	1Cys-am
1236.5317	1236.5254	5.1079	500	509	(R)GHGYVCECAR(G)	2Cys-am
1347.6705	1347.6738	-2.4624	36	46	(K)(G)
1369.6851	1369.6244	44.3215	420	430	(K)CVDLGDAYLCR(C)	2Cys-am
1488.7303	1488.7858	-37.2463	573	584	(R)LQKHRPPADPCR(G)	1Cys-am
1651.7795	1651.7168	37.9707	585	598	(R)GETETMNNLANCQR(E)	1Cys-am
1664.7759	1664.7790	-1.8284	47	61	(R)GGAGPPPCACRTFFR(V)	1Cys-am
1872.9241	1872.8741	26.6707	619	635	(K)(Y)
2211.1465	2211.1562	-4.4032	630	648	(K)(G)
2233.1048	2232.9953	49.0599	420	438	(K)CVDLGDAYLCRCQAGFSGR(H)	2Cys-am
2300.1449	2300.0381	46.4313	37	57	(K)(T)
2752.3578	2752.2466	40.3886	576	598	(K)HRPPADPCRGETETMNNLANCQR(E)	2Cys-am

The matched peptides cover 19% (142/723AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 36.6 Mean Error = 5.6207 ppm Data Tolerance = 66.8308 ppm Mean Missed Cleavages = 0.9

4. 8/41 matches (19%). 62784.2 Da, pI = 9.32. Acc. # P52737. HUMAN. ZINC FINGER PROTEIN 136.

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
1172.5447 1172.5080 31.2983 167 175 (K)(T)
1175.5307 1175.5809 -42.6982 347 356 (R)(T)
1363.6788 1363.6316 34.5650 301 311 (R)THTGEKPYECK(Q) 1Cys-am
1363.6788 1363.6316 34.5650 357 367 (R)THTGEKPYECK(E) 1Cys-am
1363.6788 1363.6316 34.5650 441 451 (R)THTGEKPYECK(Q) 1Cys-am
1370.7052 1370.6639 30.1253 497 507 (R)THTGQKPYHCK(E) 1Cys-am
2015.0876 2014.9921 47.3686 256 272 (K)(I)
2575.3463 2575.3026 16.9698 232 253 (K)(C)
2703.4317 2703.3288 38.0800 386 408 (K)HTGEGPYKCKVCGKPFHSLSPFR(I) 1Cys-am
2752.3578 2752.2724 31.0127 512 534 (K)AYSCRASFQRHMLTHAEDGPPYK(C) 1Met-ox 1Cys-am

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m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
1172.5447	1172.5080	31.2983	167	175	(K)(T)
1175.5307	1175.5809	-42.6982	347	356	(R)(T)
1363.6788	1363.6316	34.5650	301	311	(R)THTGEKPYECK(Q)	1Cys-am
1363.6788	1363.6316	34.5650	357	367	(R)THTGEKPYECK(E)	1Cys-am
1363.6788	1363.6316	34.5650	441	451	(R)THTGEKPYECK(Q)	1Cys-am
1370.7052	1370.6639	30.1253	497	507	(R)THTGQKPYHCK(E)	1Cys-am
2015.0876	2014.9921	47.3686	256	272	(K)(I)
2575.3463	2575.3026	16.9698	232	253	(K)(C)
2703.4317	2703.3288	38.0800	386	408	(K)HTGEGPYKCKVCGKPFHSLSPFR(I)	1Cys-am
2752.3578	2752.2724	31.0127	512	534	(K)AYSCRASFQRHMLTHAEDGPPYK(C)	1Met-ox 1Cys-am

The matched peptides cover 27% (148/540AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 19.5 Mean Error = 25.5851 ppm Data Tolerance = 50.2973 ppm Mean Missed Cleavages = 0.8

5. 7/41 matches (17%). 70438.9 Da, pI = 9.20. Acc. # P10078. MOUSE. ZINC FINGER PROTEIN 28 (ZFP-28) (MKR5 PROTEIN).

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
1112.4764 1112.4869 -9.4528 109 116 (K)LFQCNECK(K) 1Cys-am
1359.6560 1359.6731 -12.6001 328 338 (R)IHTGEKPFECK(V) 1Cys-am
1363.6788 1363.6316 34.5650 412 422 (K)THTGEKPYECK(E) 1Cys-am
1515.7723 1515.7266 30.1637 25 36 (R)(D)
1619.8150 1619.8505 -21.9397 117 130 (K)(I)
1872.9241 1872.8737 26.9212 216 230 (R)IHTGEKPYTCEVCHK(S) 1Cys-am
2752.3578 2752.3225 12.8016 14 36 (R)(D)

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Click individual mass to do a non-specific cleavage search.

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
1112.4764	1112.4869	-9.4528	109	116	(K)LFQCNECK(K)	1Cys-am
1359.6560	1359.6731	-12.6001	328	338	(R)IHTGEKPFECK(V)	1Cys-am
1363.6788	1363.6316	34.5650	412	422	(K)THTGEKPYECK(E)	1Cys-am
1515.7723	1515.7266	30.1637	25	36	(R)(D)
1619.8150	1619.8505	-21.9397	117	130	(K)(I)
1872.9241	1872.8737	26.9212	216	230	(R)IHTGEKPYTCEVCHK(S)	1Cys-am
2752.3578	2752.3225	12.8016	14	36	(R)(D)

The matched peptides cover 13% (82/614AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 17.1 Mean Error = 8.6370 ppm Data Tolerance = 46.1917 ppm Mean Missed Cleavages = 0.7