MS-Fit Search Results

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Sample ID (comment): HSG086
Database searched: SwissProt.12.15.2000
Molecular weight search (1000 - 100000 Da) selects 86001 entries.
Full pI range: 90939 entries.
Species search ( HUMAN MOUSE ) selects 10164 entries.
Combined molecular weight, pI and species searches select 9152 entries.
Pre searches select 9152 entries.
MS-Fit search selects 427 entries (results displayed for top 5 matches).

Parameters Used in Search

Considered modifications: | Oxidation of M | Protein N-terminus Acetylated |

4	100.000 ppm	monoisotopic	Trypsin	3	acrylamide	Hydrogen (H)	Free Acid (O H)
Min. # Peptides to Match	Peptide Mass Tolerance (+/-)	Peptide Masses are	Digest Used	Max. # Missed Cleavages	Cysteines Modified by	Peptide N terminus	Peptide C terminus

Result Summary

Rank	MOWSE Score	# (%) Masses Matched	Protein MW (Da)/pI	Species	Protein Name
1	2.07e+007	14/33 (42%)	73780.3 / 5.97	HUMAN	MITOCHONDRIAL STRESS-70 PROTEIN PRECURSOR (75 KDA GLUCOSE REGULATED PROTEIN) (GRP 75) (PEPTIDE-BINDING PROTEIN 74) (PBP74) (MORTALIN) (MOT)
2	1.15e+007	14/33 (42%)	73528.9 / 5.91	MOUSE	MITOCHONDRIAL STRESS-70 PROTEIN PRECURSOR (75 KDA GLUCOSE REGULATED PROTEIN) (GRP 75) (PEPTIDE-BINDING PROTEIN 74) (PBP74) (P66 MOT) (MORTALIN)
3	370	9/33 (27%)	86828.8 / 6.47	MOUSE	DNA-REPAIR PROTEIN COMPLEMENTING XP-D CELLS (XERODERMA PIGMENTOSUM GROUP D COMPLEMENTING PROTEIN) (DNA EXCISION REPAIR PROTEIN ERCC-2)
4	262	5/33 (15%)	64460.6 / 8.23	HUMAN	TNF RECEPTOR ASSOCIATED FACTOR 3 (CD40 RECEPTOR ASSOCIATED FACTOR 1) (CRAF1) (CD40 BINDING PROTEIN) (CD40BP) (LMP1 ASSOCIATED PROTEIN) (LAP1)
5	107	8/33 (24%)	58309.5 / 8.55	HUMAN	DNA NUCLEOTIDYLEXOTRANSFERASE (TERMINAL ADDITION ENZYME) (TERMINAL DEOXYNUCLEOTIDYLTRANSFERASE) (TERMINAL TRANSFERASE)

Detailed Results

1. 14/33 matches (42%). 73780.3 Da, pI = 5.97. Acc. # P38646. HUMAN. MITOCHONDRIAL STRESS-70 PROTEIN PRECURSOR (75 KDA GLUCOSE REGULATED PROTEIN) (GRP 75) (PEPTIDE-BINDING PROTEIN 74) (PBP74) (MORTALIN) (MOT).

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
1242.7791	1242.6806	79.2181	207	218	(K)(V)
1290.7797	1290.6806	76.7934	395	405	(K)(A)
1361.8263	1361.7429	61.2714	349	360	(R)(R)
1446.8681	1446.7626	72.8950	378	391	(K)(M)
1450.8243	1450.7178	73.4019	86	99	(R)(L)
1462.8644	1462.7576	73.0388	378	391	(K)SDIGEVILVGGMTR(M)	1Met-ox
1593.0783	1592.9528	78.7579	499	513	(K)(G)
1646.0135	1645.8801	81.0199	219	234	(R)(S)
1694.9740	1694.8502	73.0208	188	202	(K)(Q)
1867.9816	1867.8206	86.1665	596	610	(K)(L)
2056.1276	2055.9623	80.3779	266	284	(K)(H)
2092.1583	2092.0419	55.6673	53	73	(K)(Q)
2251.4208	2251.2226	88.0431	239	259	(K)(G)
2309.4250	2309.2141	91.3055	514	535	(R)(D)

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The matched peptides cover 30% (207/679AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 42.4 Mean Error = 76.4984 ppm Data Tolerance = 19.2675 ppm Mean Missed Cleavages = 0.1

2. 14/33 matches (42%). 73528.9 Da, pI = 5.91. Acc. # P38647. MOUSE. MITOCHONDRIAL STRESS-70 PROTEIN PRECURSOR (75 KDA GLUCOSE REGULATED PROTEIN) (GRP 75) (PEPTIDE-BINDING PROTEIN 74) (PBP74) (P66 MOT) (MORTALIN).

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
1242.7791 1242.6806 79.2181 207 218 (K)(V)
1290.7797 1290.6806 76.7934 395 405 (K)(A)
1446.8681 1446.7626 72.8950 378 391 (K)(M)
1450.8243 1450.7178 73.4019 86 99 (R)(L)
1462.8644 1462.7576 73.0388 378 391 (K)SDIGEVILVGGMTR(M) 1Met-ox
1593.0783 1592.9528 78.7579 499 513 (K)(G)
1646.0135 1645.8801 81.0199 219 234 (R)(S)
1694.9740 1694.8502 73.0208 188 202 (K)(Q)
1867.9816 1867.8206 86.1665 596 610 (K)(L)
2056.1276 2055.9623 80.3779 266 284 (K)(H)
2079.0951 2079.0511 21.1522 127 143 (R)(I)
2092.1583 2092.0419 55.6673 53 73 (K)(Q)
2251.4208 2251.2226 88.0431 239 259 (K)(G)
2309.4250 2309.2141 91.3055 514 535 (R)(D)

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The matched peptides cover 31% (212/679AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 42.4 Mean Error = 73.6327 ppm Data Tolerance = 34.7425 ppm Mean Missed Cleavages = 0.3

3. 9/33 matches (27%). 86828.8 Da, pI = 6.47. Acc. # O08811. MOUSE. DNA-REPAIR PROTEIN COMPLEMENTING XP-D CELLS (XERODERMA PIGMENTOSUM GROUP D COMPLEMENTING PROTEIN) (DNA EXCISION REPAIR PROTEIN ERCC-2).

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
632.3353 632.3368 -2.2642 294 299 (R)(E)
842.5771 842.5212 66.2886 287 293 (R)(E)
1446.8681 1446.8684 -0.2239 49 61 (K)(A)
1462.8644 1462.7840 54.9285 114 125 (K)(F)
1941.0867 1941.0380 25.0689 667 683 (R)(F)
2079.0951 2079.1378 -20.5437 451 469 (R)(I)
2092.1583 2092.1708 -5.9489 325 341 (R)(W)
2097.1473 2097.0513 45.7909 470 487 (K)ILDFHPVTMATFTMTLAR(V) 2Met-ox
2309.4250 2309.2076 94.1315 114 133 (K)(C)

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m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
632.3353	632.3368	-2.2642	294	299	(R)(E)
842.5771	842.5212	66.2886	287	293	(R)(E)
1446.8681	1446.8684	-0.2239	49	61	(K)(A)
1462.8644	1462.7840	54.9285	114	125	(K)(F)
1941.0867	1941.0380	25.0689	667	683	(R)(F)
2079.0951	2079.1378	-20.5437	451	469	(R)(I)
2092.1583	2092.1708	-5.9489	325	341	(R)(W)
2097.1473	2097.0513	45.7909	470	487	(K)ILDFHPVTMATFTMTLAR(V)	2Met-ox
2309.4250	2309.2076	94.1315	114	133	(K)(C)

The matched peptides cover 15% (117/760AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 27.3 Mean Error = 28.5809 ppm Data Tolerance = 77.8079 ppm Mean Missed Cleavages = 0.9

4. 5/33 matches (15%). 64460.6 Da, pI = 8.23. Acc. # Q13114. HUMAN. TNF RECEPTOR ASSOCIATED FACTOR 3 (CD40 RECEPTOR ASSOCIATED FACTOR 1) (CRAF1) (CD40 BINDING PROTEIN) (CD40BP) (LMP1 ASSOCIATED PROTEIN) (LAP1).

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
1350.7764 1350.6588 87.0087 388 398 (R)(L)
1468.8721 1468.7470 85.2133 7 20 (K)(L)
1484.8408 1484.7419 66.5834 7 20 (K)MDSPGALQTNPPLK(L) 1Met-ox
1867.9816 1867.9958 -7.6244 406 421 (R)(I)
2705.3693 2705.4011 -11.7542 250 273 (K)(V)

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m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
1350.7764	1350.6588	87.0087	388	398	(R)(L)
1468.8721	1468.7470	85.2133	7	20	(K)(L)
1484.8408	1484.7419	66.5834	7	20	(K)MDSPGALQTNPPLK(L)	1Met-ox
1867.9816	1867.9958	-7.6244	406	421	(R)(I)
2705.3693	2705.4011	-11.7542	250	273	(K)(V)

The matched peptides cover 11% (65/568AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 15.2 Mean Error = 43.8854 ppm Data Tolerance = 99.1556 ppm Mean Missed Cleavages = 0.4

5. 8/33 matches (24%). 58309.5 Da, pI = 8.55. Acc. # P04053. HUMAN. DNA NUCLEOTIDYLEXOTRANSFERASE (TERMINAL ADDITION ENZYME) (TERMINAL DEOXYNUCLEOTIDYLTRANSFERASE) (TERMINAL TRANSFERASE).

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
1350.7764 1350.7673 6.6984 32 42 (K)(K)
1361.8263 1361.8116 10.7853 273 284 (R)(F)
1446.8681 1446.7263 98.0470 18 31 (R)(F)
1462.8644 1462.7212 97.9159 18 31 (R)QTGALMASSPQDIK(F) 1Met-ox
1468.8721 1468.7946 52.7901 279 290 (R)(A)
1484.8408 1484.7895 34.5101 279 290 (R)SDKSLKFTRMQK(A) 1Met-ox
1617.9734 1617.8787 58.5736 429 441 (K)(R)
2705.3693 2705.2616 39.8061 340 363 (K)MGHDVDFLITSPGSTEDEEQLLQK(V) 1Met-ox

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m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
1350.7764	1350.7673	6.6984	32	42	(K)(K)
1361.8263	1361.8116	10.7853	273	284	(R)(F)
1446.8681	1446.7263	98.0470	18	31	(R)(F)
1462.8644	1462.7212	97.9159	18	31	(R)QTGALMASSPQDIK(F)	1Met-ox
1468.8721	1468.7946	52.7901	279	290	(R)(A)
1484.8408	1484.7895	34.5101	279	290	(R)SDKSLKFTRMQK(A)	1Met-ox
1617.9734	1617.8787	58.5736	429	441	(K)(R)
2705.3693	2705.2616	39.8061	340	363	(K)MGHDVDFLITSPGSTEDEEQLLQK(V)	1Met-ox

The matched peptides cover 15% (80/508AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 24.2 Mean Error = 49.8908 ppm Data Tolerance = 69.4563 ppm Mean Missed Cleavages = 1.3