MS-Fit Search Results

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Sample ID (comment): HSG087
Database searched: SwissProt.12.15.2000
Molecular weight search (1000 - 100000 Da) selects 86001 entries.
Full pI range: 90939 entries.
Species search ( HUMAN MOUSE ) selects 10164 entries.
Combined molecular weight, pI and species searches select 9152 entries.
Pre searches select 9152 entries.
MS-Fit search selects 178 entries (results displayed for top 5 matches).

Parameters Used in Search

Considered modifications: | Oxidation of M | Protein N-terminus Acetylated |

4	50.000 ppm	monoisotopic	Trypsin	3	acrylamide	Hydrogen (H)	Free Acid (O H)
Min. # Peptides to Match	Peptide Mass Tolerance (+/-)	Peptide Masses are	Digest Used	Max. # Missed Cleavages	Cysteines Modified by	Peptide N terminus	Peptide C terminus

Result Summary

Rank	MOWSE Score	# (%) Masses Matched	Protein MW (Da)/pI	Species	Protein Name
1	3.24e+007	14/31 (45%)	70871.6 / 5.38	MOUSE	HEAT SHOCK COGNATE 71 KDA PROTEIN
2	3.23e+007	14/31 (45%)	70898.7 / 5.38	HUMAN	HEAT SHOCK COGNATE 71 KDA PROTEIN
3	443	8/31 (25%)	70021.5 / 5.56	HUMAN	HEAT SHOCK-RELATED 70 KD PROTEIN 2 (HEAT SHOCK 70 KD PROTEIN 2)
4	291	5/31 (16%)	68264.7 / 5.35	HUMAN	VACUOLAR ATP SYNTHASE CATALYTIC SUBUNIT A, UBIQUITOUS ISOFORM (V-ATPASE 69 KD SUBUNIT) (ISOFORM VA68)
5	182	5/31 (16%)	89278.5 / 6.83	HUMAN	DNA-REPAIR PROTEIN COMPLEMENTING XP-B CELLS (XERODERMA PIGMENTOSUM GROUP B COMPLEMENTING PROTEIN) (DNA EXCISION REPAIR PROTEIN ERCC-3) (BASAL TRANSCRIPTION FACTOR 2 89 KDA SUBUNIT) (BTF2-P89) (TFIIH 89 KDA SUBUNIT)

Detailed Results

1. 14/31 matches (45%). 70871.6 Da, pI = 5.38. Acc. # P08109. MOUSE. HEAT SHOCK COGNATE 71 KDA PROTEIN.

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
1199.7190	1199.6748	36.8637	160	171	(K)(I)
1253.6443	1253.6166	22.0562	302	311	(R)(G)
1480.7724	1480.7549	11.8259	300	311	(R)(G)
1487.7215	1487.7018	13.2465	37	49	(R)(L)
1653.8183	1653.8324	-8.4916	89	102	(K)(V)
1669.8754	1669.8273	28.8178	89	102	(K)HWPFMVVNDAGRPK(V)	1Met-ox
1691.7496	1691.7261	13.8749	221	236	(K)(M)
1745.8040	1745.8095	-3.1075	584	597	(K)(E)
1982.0132	1981.9983	7.5007	138	155	(K)(Q)
1982.0132	1981.9653	24.1584	518	533	(R)(D)
2260.1869	2260.1461	18.0488	362	384	(K)(S)
2277.1433	2277.1338	4.1885	4	25	(K)(V)
2514.2996	2514.3092	-3.8100	470	493	(R)(S)
2774.3279	2774.3273	0.1929	424	447	(K)(A)
2997.4146	2997.4581	-14.5140	273	299	(R)(A)

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The matched peptides cover 36% (235/646AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 45.2 Mean Error = 10.0567 ppm Data Tolerance = 29.0655 ppm Mean Missed Cleavages = 0.3

2. 14/31 matches (45%). 70898.7 Da, pI = 5.38. Acc. # P11142. HUMAN. HEAT SHOCK COGNATE 71 KDA PROTEIN.

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
1199.7190 1199.6748 36.8637 160 171 (K)(I)
1253.6443 1253.6166 22.0562 302 311 (R)(G)
1480.7724 1480.7549 11.8259 300 311 (R)(G)
1487.7215 1487.7018 13.2465 37 49 (R)(L)
1653.8183 1653.8324 -8.4916 89 102 (K)(V)
1669.8754 1669.8273 28.8178 89 102 (K)HWPFMVVNDAGRPK(V) 1Met-ox
1691.7496 1691.7261 13.8749 221 236 (K)(M)
1745.8040 1745.8095 -3.1075 584 597 (K)(E)
1982.0132 1981.9983 7.5007 138 155 (K)(Q)
1982.0132 1981.9653 24.1584 518 533 (R)(D)
2260.1869 2260.1461 18.0488 362 384 (K)(S)
2277.1433 2277.1338 4.1885 4 25 (K)(V)
2514.2996 2514.3092 -3.8100 470 493 (R)(S)
2774.3279 2774.3273 0.1929 424 447 (K)(A)
2997.4146 2997.4581 -14.5140 273 299 (R)(A)

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Click individual mass to do a non-specific cleavage search.

The matched peptides cover 36% (235/646AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 45.2 Mean Error = 10.0567 ppm Data Tolerance = 29.0655 ppm Mean Missed Cleavages = 0.3

3. 8/31 matches (25%). 70021.5 Da, pI = 5.56. Acc. # P54652. HUMAN. HEAT SHOCK-RELATED 70 KD PROTEIN 2 (HEAT SHOCK 70 KD PROTEIN 2).

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
1253.6443 1253.6166 22.0562 305 314 (R)(G)
1480.7724 1480.7549 11.8259 303 314 (R)(G)
1487.7215 1487.7018 13.2465 38 50 (R)(L)
1691.7496 1691.7261 13.8749 224 239 (K)(M)
1819.8517 1819.9125 -33.4233 58 73 (K)(L)
1993.9211 1993.9231 -0.9825 79 94 (K)(V)
2514.2996 2514.2728 10.6615 539 560 (R)(L)
2757.2869 2757.3444 -20.8454 530 553 (K)(Q)

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Click individual mass to do a non-specific cleavage search.

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)
1253.6443	1253.6166	22.0562	305	314	(R)(G)
1480.7724	1480.7549	11.8259	303	314	(R)(G)
1487.7215	1487.7018	13.2465	38	50	(R)(L)
1691.7496	1691.7261	13.8749	224	239	(K)(M)
1819.8517	1819.9125	-33.4233	58	73	(K)(L)
1993.9211	1993.9231	-0.9825	79	94	(K)(V)
2514.2996	2514.2728	10.6615	539	560	(R)(L)
2757.2869	2757.3444	-20.8454	530	553	(K)(Q)

The matched peptides cover 16% (104/639AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 25.8 Mean Error = 2.0517 ppm Data Tolerance = 38.7439 ppm Mean Missed Cleavages = 1.0

4. 5/31 matches (16%). 68264.7 Da, pI = 5.35. Acc. # P38606. HUMAN. VACUOLAR ATP SYNTHASE CATALYTIC SUBUNIT A, UBIQUITOUS ISOFORM (V-ATPASE 69 KD SUBUNIT) (ISOFORM VA68).

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
1745.8040 1745.8169 -7.3425 266 280 (K)(G)
1749.8440 1749.8699 -14.8240 324 338 (R)(D)
1800.7762 1800.8227 -25.7990 599 613 (K)(S)
1819.8517 1819.8545 -1.5625 537 552 (K)(R)
2143.0682 2143.0500 8.4922 460 476 (R)(T)

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Click individual mass to do a non-specific cleavage search.

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)
1745.8040	1745.8169	-7.3425	266	280	(K)(G)
1749.8440	1749.8699	-14.8240	324	338	(R)(D)
1800.7762	1800.8227	-25.7990	599	613	(K)(S)
1819.8517	1819.8545	-1.5625	537	552	(K)(R)
2143.0682	2143.0500	8.4922	460	476	(R)(T)

The matched peptides cover 12% (78/617AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 16.1 Mean Error = -8.2072 ppm Data Tolerance = 26.0197 ppm Mean Missed Cleavages = 0.2

5. 5/31 matches (16%). 89278.5 Da, pI = 6.83. Acc. # P19447. HUMAN. DNA-REPAIR PROTEIN COMPLEMENTING XP-B CELLS (XERODERMA PIGMENTOSUM GROUP B COMPLEMENTING PROTEIN) (DNA EXCISION REPAIR PROTEIN ERCC-3) (BASAL TRANSCRIPTION FACTOR 2 89 KDA SUBUNIT) (BTF2-P89) (TFIIH 89 KDA SUBUNIT).

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
1487.7215 1487.7647 -29.0155 677 688 (R)(V)
1982.0132 1982.1115 -49.6000 558 574 (K)(L)
1982.0132 1982.0156 -1.2225 689 706 (K)(E)
1993.9211 1993.8563 32.4776 242 257 (K)(D)
2757.2869 2757.3823 -34.5921 95 117 (K)(L)
2997.4146 2997.4899 -25.1393 69 94 (K)(Y)

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Click individual mass to do a non-specific cleavage search.

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)
1487.7215	1487.7647	-29.0155	677	688	(R)(V)
1982.0132	1982.1115	-49.6000	558	574	(K)(L)
1982.0132	1982.0156	-1.2225	689	706	(K)(E)
1993.9211	1993.8563	32.4776	242	257	(K)(D)
2757.2869	2757.3823	-34.5921	95	117	(K)(L)
2997.4146	2997.4899	-25.1393	69	94	(K)(Y)

The matched peptides cover 14% (112/782AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 16.1 Mean Error = -17.8486 ppm Data Tolerance = 58.4842 ppm Mean Missed Cleavages = 0.5