MS-Fit Search Results

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Sample ID (comment): HSG088
Database searched: SwissProt.12.15.2000
Molecular weight search (1000 - 100000 Da) selects 86001 entries.
Full pI range: 90939 entries.
Species search ( HUMAN MOUSE ) selects 10164 entries.
Combined molecular weight, pI and species searches select 9152 entries.
Pre searches select 9152 entries.
MS-Fit search selects 112 entries (results displayed for top 5 matches).

Parameters Used in Search

Considered modifications: | Oxidation of M | Protein N-terminus Acetylated |

4	50.000 ppm	monoisotopic	Trypsin	3	acrylamide	Hydrogen (H)	Free Acid (O H)
Min. # Peptides to Match	Peptide Mass Tolerance (+/-)	Peptide Masses are	Digest Used	Max. # Missed Cleavages	Cysteines Modified by	Peptide N terminus	Peptide C terminus

Result Summary

Rank	MOWSE Score	# (%) Masses Matched	Protein MW (Da)/pI	Species	Protein Name
1	8.09e+007	19/31 (61%)	72116.2 / 5.03	HUMAN	78 KD GLUCOSE-REGULATED PROTEIN PRECURSOR (GRP 78) (IMMUNOGLOBULIN HEAVY CHAIN BINDING PROTEIN) (BIP)
2	9.88e+006	17/31 (54%)	72422.6 / 5.07	MOUSE	78 KD GLUCOSE-REGULATED PROTEIN PRECURSOR (GRP 78) (IMMUNOGLOBULIN HEAVY CHAIN BINDING PROTEIN) (BIP)
3	236	5/31 (16%)	24871.6 / 5.70	HUMAN	RAS-RELATED PROTEIN RAB-28 (RAB-26)
4	115	4/31 (12%)	10071.5 / 8.96	MOUSE	CYTOCHROME C OXIDASE POLYPEPTIDE VIB (AED)
5	99.1	5/31 (16%)	42864.8 / 6.06	HUMAN	CYTOPLASMIC PROTEIN NCK1 (NCK ADAPTOR PROTEIN 1) (SH2/SH3 ADAPTOR PROTEIN NCK-ALPHA)

Detailed Results

1. 19/31 matches (61%). 72116.2 Da, pI = 5.03. Acc. # P11021. HUMAN. 78 KD GLUCOSE-REGULATED PROTEIN PRECURSOR (GRP 78) (IMMUNOGLOBULIN HEAVY CHAIN BINDING PROTEIN) (BIP).

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
986.4801	986.5159	-36.2055	532	539	(R)(M)
1191.5994	1191.6374	-31.8700	464	473	(K)(D)
1430.6470	1430.6916	-31.1443	102	113	(R)(F)
1460.7163	1460.7597	-29.7006	353	366	(K)(I)
1512.7153	1512.7521	-24.3223	324	335	(R)(S)
1528.7030	1528.7470	-28.7806	324	335	(R)AKFEELNMDLFR(S)	1Met-ox
1536.7549	1536.7984	-28.2803	139	152	(K)(M)
1552.7226	1552.7933	-45.5178	139	152	(K)TFAPEEISAMVLTK(M)	1Met-ox
1566.7325	1566.7804	-30.5951	61	74	(R)(L)
1588.8074	1588.8546	-29.7324	352	366	(K)(I)
1604.7894	1604.8648	-46.9767	124	138	(K)(T)
1815.9319	1815.9969	-35.7880	198	214	(R)(E)
1887.9028	1887.9717	-36.4856	165	181	(K)(Q)
1933.9588	1934.0136	-28.3052	474	491	(K)(G)
1974.8574	1974.9085	-25.9047	601	616	(K)(A)
2015.9704	2016.0667	-47.7613	164	181	(K)(Q)
2164.9297	2164.9926	-29.0542	306	323	(R)(A)
2173.9511	2174.0406	-41.1673	601	618	(K)(K)
2175.9309	2175.9934	-28.7144	633	653	(K)(-)

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The matched peptides cover 32% (210/653AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 61.3 Mean Error = -33.4898 ppm Data Tolerance = 14.2677 ppm Mean Missed Cleavages = 0.4

2. 17/31 matches (54%). 72422.6 Da, pI = 5.07. Acc. # P20029. MOUSE. 78 KD GLUCOSE-REGULATED PROTEIN PRECURSOR (GRP 78) (IMMUNOGLOBULIN HEAVY CHAIN BINDING PROTEIN) (BIP).

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
986.4801 986.5159 -36.2055 534 541 (R)(M)
1191.5994 1191.6374 -31.8700 466 475 (K)(D)
1430.6470 1430.6916 -31.1443 103 114 (R)(F)
1460.7163 1460.7597 -29.7006 355 368 (K)(I)
1512.7153 1512.7521 -24.3223 326 337 (R)(S)
1528.7030 1528.7470 -28.7806 326 337 (R)AKFEELNMDLFR(S) 1Met-ox
1536.7549 1536.7984 -28.2803 140 153 (K)(M)
1552.7226 1552.7933 -45.5178 140 153 (K)TFAPEEISAMVLTK(M) 1Met-ox
1566.7325 1566.7804 -30.5951 62 75 (R)(L)
1588.8074 1588.8546 -29.7324 354 368 (K)(I)
1604.7894 1604.8648 -46.9767 125 139 (K)(T)
1815.9319 1815.9969 -35.7880 199 215 (R)(E)
1887.9028 1887.9717 -36.4856 166 182 (K)(Q)
1933.9588 1934.0136 -28.3052 476 493 (K)(G)
1974.8574 1974.9085 -25.9047 603 618 (K)(A)
2015.9704 2016.0667 -47.7613 165 182 (K)(Q)
2173.9511 2174.0406 -41.1673 603 620 (K)(K)

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m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
986.4801	986.5159	-36.2055	534	541	(R)(M)
1191.5994	1191.6374	-31.8700	466	475	(K)(D)
1430.6470	1430.6916	-31.1443	103	114	(R)(F)
1460.7163	1460.7597	-29.7006	355	368	(K)(I)
1512.7153	1512.7521	-24.3223	326	337	(R)(S)
1528.7030	1528.7470	-28.7806	326	337	(R)AKFEELNMDLFR(S)	1Met-ox
1536.7549	1536.7984	-28.2803	140	153	(K)(M)
1552.7226	1552.7933	-45.5178	140	153	(K)TFAPEEISAMVLTK(M)	1Met-ox
1566.7325	1566.7804	-30.5951	62	75	(R)(L)
1588.8074	1588.8546	-29.7324	354	368	(K)(I)
1604.7894	1604.8648	-46.9767	125	139	(K)(T)
1815.9319	1815.9969	-35.7880	199	215	(R)(E)
1887.9028	1887.9717	-36.4856	166	182	(K)(Q)
1933.9588	1934.0136	-28.3052	476	493	(K)(G)
1974.8574	1974.9085	-25.9047	603	618	(K)(A)
2015.9704	2016.0667	-47.7613	165	182	(K)(Q)
2173.9511	2174.0406	-41.1673	603	620	(K)(K)

The matched peptides cover 26% (171/655AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 54.8 Mean Error = -34.0316 ppm Data Tolerance = 14.7358 ppm Mean Missed Cleavages = 0.4

3. 5/31 matches (16%). 24871.6 Da, pI = 5.70. Acc. # P51157. HUMAN. RAS-RELATED PROTEIN RAB-28 (RAB-26).

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
1460.7163 1460.6990 11.8326 209 221 (R)(-)
1536.7549 1536.7117 28.1306 196 208 (K)(T)
1552.7226 1552.7066 10.3110 196 208 (K)ADIVNYNQEPMSR(T) 1Met-ox
1604.7894 1604.7630 16.4334 26 39 (K)(Q)
2164.9297 2165.0351 -48.6519 144 161 (K)(T)

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m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
1460.7163	1460.6990	11.8326	209	221	(R)(-)
1536.7549	1536.7117	28.1306	196	208	(K)(T)
1552.7226	1552.7066	10.3110	196	208	(K)ADIVNYNQEPMSR(T)	1Met-ox
1604.7894	1604.7630	16.4334	26	39	(K)(Q)
2164.9297	2165.0351	-48.6519	144	161	(K)(T)

The matched peptides cover 26% (58/221AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 16.1 Mean Error = 3.6111 ppm Data Tolerance = 60.0793 ppm Mean Missed Cleavages = 0.4

4. 4/31 matches (12%). 10071.5 Da, pI = 8.96. Acc. # P56391. MOUSE. CYTOCHROME C OXIDASE POLYPEPTIDE VIB (AED).

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
1566.7325 1566.6912 26.3334 29 39 (K)(C)
1887.9028 1887.9176 -7.8074 48 62 (K)(S)
1999.9916 1999.9482 21.6836 43 59 (K)(V)
2015.9704 2015.9431 13.5194 43 59 (K)AMTAKGGDVSVCEWYRR(V) 1Met-ox

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m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
1566.7325	1566.6912	26.3334	29	39	(K)(C)
1887.9028	1887.9176	-7.8074	48	62	(K)(S)
1999.9916	1999.9482	21.6836	43	59	(K)(V)
2015.9704	2015.9431	13.5194	43	59	(K)AMTAKGGDVSVCEWYRR(V)	1Met-ox

The matched peptides cover 36% (31/86AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 12.9 Mean Error = 13.4322 ppm Data Tolerance = 30.2359 ppm Mean Missed Cleavages = 1.5

5. 5/31 matches (16%). 42864.8 Da, pI = 6.06. Acc. # P16333. HUMAN. CYTOPLASMIC PROTEIN NCK1 (NCK ADAPTOR PROTEIN 1) (SH2/SH3 ADAPTOR PROTEIN NCK-ALPHA).

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
1512.7153 1512.7045 7.1562 346 357 (K)(K)
1528.7030 1528.6994 2.3685 346 357 (K)FSTMEELVEHYK(K) 1Met-ox
1570.8028 1570.7760 17.0827 287 299 (K)VTRHQAEMALNER(G) 1Met-ox
1604.7894 1604.8225 -20.6455 29 40 (R)(V)
2186.9044 2187.0068 -46.8244 214 231 (K)(C)

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m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
1512.7153	1512.7045	7.1562	346	357	(K)(K)
1528.7030	1528.6994	2.3685	346	357	(K)FSTMEELVEHYK(K)	1Met-ox
1570.8028	1570.7760	17.0827	287	299	(K)VTRHQAEMALNER(G)	1Met-ox
1604.7894	1604.8225	-20.6455	29	40	(R)(V)
2186.9044	2187.0068	-46.8244	214	231	(K)(C)

The matched peptides cover 14% (55/377AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 16.1 Mean Error = -8.1725 ppm Data Tolerance = 51.3172 ppm Mean Missed Cleavages = 0.4