MS-Fit Search Results

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Sample ID (comment): HSG091
Database searched: SwissProt.1.20.2002
Molecular weight search (1000 - 100000 Da) selects 98142 entries.
Full pI range: 103987 entries.
Species search ( HUMAN MOUSE ) selects 12504 entries.
Combined molecular weight, pI and species searches select 11102 entries.
Pre searches select 11102 entries.
MS-Fit search selects 1585 entries (results displayed for top 5 matches).

Parameters Used in Search

Considered modifications: | Oxidation of M | Protein N-terminus Acetylated | Acrylamide Modified Cys |

4	100.000 ppm	monoisotopic	Trypsin	3	carbamidomethylation	Hydrogen (H)	Free Acid (O H)
Min. # Peptides to Match	Peptide Mass Tolerance (+/-)	Peptide Masses are	Digest Used	Max. # Missed Cleavages	Cysteines Modified by	Peptide N terminus	Peptide C terminus

Result Summary

Rank	MOWSE Score	# (%) Masses Matched	Protein MW (Da)/pI	Species	Protein Name
1	2.95e+006	14/43 (32%)	47033.3 / 5.30	HUMAN	CARGO SELECTION PROTEIN TIP47 (47 KDA MANNOSE 6-PHOSPHATE RECEPTOR-BINDING PROTEIN) (47 KDA MPR-BINDING PROTEIN) (PLACENTAL PROTEIN 17)
2	1.57e+003	6/43 (13%)	48252.4 / 6.78	HUMAN	Sialidase 3 (Membrane sialidase) (Ganglioside sialidase) (N-acetyl-alpha-neuraminidase 3)
3	1.16e+003	11/43 (25%)	67255.0 / 9.06	MOUSE	ZINC FINGER PROTEIN 37 (ZFP-37) (MALE GERM CELL SPECIFIC ZINC FINGER PROTEIN)
4	1.11e+003	11/43 (25%)	76839.7 / 6.57	HUMAN	PROTEIN KINASE C, BETA-I TYPE (PKC-BETA-1)
5	1.11e+003	11/43 (25%)	77012.8 / 6.59	HUMAN	PROTEIN KINASE C, BETA-II TYPE (PKC-BETA-2)

Detailed Results

1. 14/43 matches (32%). 47033.3 Da, pI = 5.30. Acc. # O60664. HUMAN. CARGO SELECTION PROTEIN TIP47 (47 KDA MANNOSE 6-PHOSPHATE RECEPTOR-BINDING PROTEIN) (47 KDA MPR-BINDING PROTEIN) (PLACENTAL PROTEIN 17).

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
1056.4310	1056.5114	-76.1228	231	238	(R)(L)
1363.6008	1363.7004	-73.0229	167	180	(K)(L)
1438.6414	1438.7555	-79.3356	246	257	(R)(L)
1438.6414	1438.7555	-79.3356	248	259	(R)(A)
1470.6240	1470.7316	-73.2016	297	307	(K)(Q)
1486.6634	1486.7265	-42.4884	297	307	(K)LHQMWLSWNQK(Q)	1Met-ox
1571.7116	1571.7818	-44.6339	85	98	(K)(G)
1576.6925	1576.7819	-56.6834	141	155	(K)(G)
1834.8391	1834.9299	-49.4698	214	230	(R)(Q)
2065.0068	2065.1181	-53.8852	99	116	(R)(V)
2066.9431	2066.9783	-17.0497	196	213	(K)(I)
2073.1005	2073.1266	-12.5861	265	283	(R)(Q)
2076.0050	2076.0726	-32.5331	308	325	(K)(A)
2813.2829	2813.3077	-8.8329	1	28	(-)SADGAEADGSTQVTVEEPVQQPSVVDR(V)	Acet N
2994.4175	2994.4809	-21.1654	365	391	(R)(E)

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The matched peptides cover 50% (221/434AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 32.6 Mean Error = -48.0231 ppm Data Tolerance = 50.3569 ppm Mean Missed Cleavages = 0.3

2. 6/43 matches (13%). 48252.4 Da, pI = 6.78. Acc. # Q9UQ49. HUMAN. Sialidase 3 (Membrane sialidase) (Ganglioside sialidase) (N-acetyl-alpha-neuraminidase 3).

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
1834.8391 1834.9023 -34.3992 392 405 (K)(E)
2058.9696 2059.0680 -47.8016 217 234 (R)(A)
2073.1005 2073.0837 8.1126 217 234 (R)LIRPMVTVECEVAEVTGR(A) 1Cys-am
2075.0233 2075.0629 -19.0830 217 234 (R)LIRPMVTVECEVAEVTGR(A) 1Met-ox
2384.9515 2385.1621 -88.2964 406 426 (R)EILSHLQGDCTSPGRNPSQFK(S) 1Cys-am
2813.2829 2813.3875 -37.2092 174 195 (R)(T)

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m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
1834.8391	1834.9023	-34.3992	392	405	(K)(E)
2058.9696	2059.0680	-47.8016	217	234	(R)(A)
2073.1005	2073.0837	8.1126	217	234	(R)LIRPMVTVECEVAEVTGR(A)	1Cys-am
2075.0233	2075.0629	-19.0830	217	234	(R)LIRPMVTVECEVAEVTGR(A)	1Met-ox
2384.9515	2385.1621	-88.2964	406	426	(R)EILSHLQGDCTSPGRNPSQFK(S)	1Cys-am
2813.2829	2813.3875	-37.2092	174	195	(R)(T)

The matched peptides cover 17% (75/428AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 14.0 Mean Error = -36.4461 ppm Data Tolerance = 63.9320 ppm Mean Missed Cleavages = 0.3

3. 11/43 matches (25%). 67255.0 Da, pI = 9.06. Acc. # P17141. MOUSE. ZINC FINGER PROTEIN 37 (ZFP-37) (MALE GERM CELL SPECIFIC ZINC FINGER PROTEIN).

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
868.4510 868.4416 10.8208 26 32 (R)(L)
1250.5755 1250.6493 -59.0222 131 141 (K)(K)
1363.6008 1363.6164 -11.4233 1 13 (-)(A)
1834.8391 1834.8580 -10.2847 332 346 (R)IHTGEKPYKCNECGK(T) 1Cys-am
1838.7785 1838.7260 28.5608 472 486 (R)THTGDCPFECNQCGK(A) 2Cys-am
2058.9696 2059.0559 -41.9246 49 67 (K)(A)
2211.0431 2211.0940 -23.0500 216 234 (K)(R)
2283.0700 2283.1078 -16.5491 152 171 (K)(K)
2283.0700 2283.0538 7.0860 416 434 (R)IHTGEKPFECNECGKTFSK(K) 1Cys-am
2299.0912 2299.1278 -15.9461 81 100 (K)(S)
2813.2829 2813.3252 -15.0397 520 542 (K)(A)
2994.4175 2994.4024 5.0255 406 430 (K)YGSSLTKHMRIHTGEKPFECNECGK(T) 2Cys-am

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m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
868.4510	868.4416	10.8208	26	32	(R)(L)
1250.5755	1250.6493	-59.0222	131	141	(K)(K)
1363.6008	1363.6164	-11.4233	1	13	(-)(A)
1834.8391	1834.8580	-10.2847	332	346	(R)IHTGEKPYKCNECGK(T)	1Cys-am
1838.7785	1838.7260	28.5608	472	486	(R)THTGDCPFECNQCGK(A)	2Cys-am
2058.9696	2059.0559	-41.9246	49	67	(K)(A)
2211.0431	2211.0940	-23.0500	216	234	(K)(R)
2283.0700	2283.1078	-16.5491	152	171	(K)(K)
2283.0700	2283.0538	7.0860	416	434	(R)IHTGEKPFECNECGKTFSK(K)	1Cys-am
2299.0912	2299.1278	-15.9461	81	100	(K)(S)
2813.2829	2813.3252	-15.0397	520	542	(K)(A)
2994.4175	2994.4024	5.0255	406	430	(K)YGSSLTKHMRIHTGEKPFECNECGK(T)	2Cys-am

The matched peptides cover 32% (191/594AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 25.6 Mean Error = -11.8122 ppm Data Tolerance = 47.0865 ppm Mean Missed Cleavages = 1.4

4. 11/43 matches (25%). 76839.7 Da, pI = 6.57. Acc. # P05771. HUMAN. PROTEIN KINASE C, BETA-I TYPE (PKC-BETA-1).

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
1470.6240 1470.7263 -69.5573 469 481 (K)(I)
1486.6634 1486.7212 -38.8832 469 481 (K)LDNVMLDSEGHIK(I) 1Met-ox
1486.6634 1486.7436 -53.9940 583 595 (K)(E)
1838.7785 1838.8504 -39.0990 63 76 (K)QGFQCQVCCFVVHK(R) 3Cys-am
1838.7785 1838.8318 -28.9696 77 91 (K)RCHEFVTFSCPGADK(G) 2Cys-am
1838.7785 1838.8745 -52.2048 321 336 (K)(M)
2065.0068 2064.9582 23.5600 376 392 (K)DVVIQDDDVECTMVEKR(V) 1Cys-am
2066.9431 2066.9374 2.7267 376 392 (K)DVVIQDDDVECTMVEKR(V) 1Met-ox
2073.1005 2073.0327 32.7095 179 197 (R)(L)
2076.0050 2075.9425 30.1204 142 158 (K)RCVMNVPSLCGTDHTER(R) 1Met-ox 2Cys-am
2076.0050 2075.9425 30.1204 143 159 (R)CVMNVPSLCGTDHTERR(G) 1Met-ox 2Cys-am
2082.9766 2083.0071 -14.6548 217 232 (K)CSLNPEWNETFRFQLK(E) 1Cys-am
2088.0648 2087.9108 73.7585 46 62 (K)(Q)
2097.8713 2098.0100 -66.0910 321 338 (K)(L)
2813.2829 2813.4004 -41.7946 1 27 (-)MADPAAGPPPSEGEESTVRFARKGALR(Q) 1Met-ox
2813.2829 2813.3317 -17.3590 217 238 (K)CSLNPEWNETFRFQLKESDKDR(R) 1Cys-am

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m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
1470.6240	1470.7263	-69.5573	469	481	(K)(I)
1486.6634	1486.7212	-38.8832	469	481	(K)LDNVMLDSEGHIK(I)	1Met-ox
1486.6634	1486.7436	-53.9940	583	595	(K)(E)
1838.7785	1838.8504	-39.0990	63	76	(K)QGFQCQVCCFVVHK(R)	3Cys-am
1838.7785	1838.8318	-28.9696	77	91	(K)RCHEFVTFSCPGADK(G)	2Cys-am
1838.7785	1838.8745	-52.2048	321	336	(K)(M)
2065.0068	2064.9582	23.5600	376	392	(K)DVVIQDDDVECTMVEKR(V)	1Cys-am
2066.9431	2066.9374	2.7267	376	392	(K)DVVIQDDDVECTMVEKR(V)	1Met-ox
2073.1005	2073.0327	32.7095	179	197	(R)(L)
2076.0050	2075.9425	30.1204	142	158	(K)RCVMNVPSLCGTDHTER(R)	1Met-ox 2Cys-am
2076.0050	2075.9425	30.1204	143	159	(R)CVMNVPSLCGTDHTERR(G)	1Met-ox 2Cys-am
2082.9766	2083.0071	-14.6548	217	232	(K)CSLNPEWNETFRFQLK(E)	1Cys-am
2088.0648	2087.9108	73.7585	46	62	(K)(Q)
2097.8713	2098.0100	-66.0910	321	338	(K)(L)
2813.2829	2813.4004	-41.7946	1	27	(-)MADPAAGPPPSEGEESTVRFARKGALR(Q)	1Met-ox
2813.2829	2813.3317	-17.3590	217	238	(K)CSLNPEWNETFRFQLKESDKDR(R)	1Cys-am

The matched peptides cover 28% (193/671AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 25.6 Mean Error = -14.3507 ppm Data Tolerance = 84.1931 ppm Mean Missed Cleavages = 1.3

5. 11/43 matches (25%). 77012.8 Da, pI = 6.59. Acc. # P05127. HUMAN. PROTEIN KINASE C, BETA-II TYPE (PKC-BETA-2).

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
1470.6240 1470.7263 -69.5573 469 481 (K)(I)
1486.6634 1486.7212 -38.8832 469 481 (K)LDNVMLDSEGHIK(I) 1Met-ox
1486.6634 1486.7436 -53.9940 583 595 (K)(E)
1838.7785 1838.8504 -39.0990 63 76 (K)QGFQCQVCCFVVHK(R) 3Cys-am
1838.7785 1838.8318 -28.9696 77 91 (K)RCHEFVTFSCPGADK(G) 2Cys-am
1838.7785 1838.8745 -52.2048 321 336 (K)(M)
2065.0068 2064.9582 23.5600 376 392 (K)DVVIQDDDVECTMVEKR(V) 1Cys-am
2066.9431 2066.9374 2.7267 376 392 (K)DVVIQDDDVECTMVEKR(V) 1Met-ox
2073.1005 2073.0327 32.7095 179 197 (R)(L)
2076.0050 2075.9425 30.1204 142 158 (K)RCVMNVPSLCGTDHTER(R) 1Met-ox 2Cys-am
2076.0050 2075.9425 30.1204 143 159 (R)CVMNVPSLCGTDHTERR(G) 1Met-ox 2Cys-am
2082.9766 2083.0071 -14.6548 217 232 (K)CSLNPEWNETFRFQLK(E) 1Cys-am
2088.0648 2087.9108 73.7585 46 62 (K)(Q)
2097.8713 2098.0100 -66.0910 321 338 (K)(L)
2813.2829 2813.4004 -41.7946 1 27 (-)MADPAAGPPPSEGEESTVRFARKGALR(Q) 1Met-ox
2813.2829 2813.3317 -17.3590 217 238 (K)CSLNPEWNETFRFQLKESDKDR(R) 1Cys-am

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Click individual mass to do a non-specific cleavage search.

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
1470.6240	1470.7263	-69.5573	469	481	(K)(I)
1486.6634	1486.7212	-38.8832	469	481	(K)LDNVMLDSEGHIK(I)	1Met-ox
1486.6634	1486.7436	-53.9940	583	595	(K)(E)
1838.7785	1838.8504	-39.0990	63	76	(K)QGFQCQVCCFVVHK(R)	3Cys-am
1838.7785	1838.8318	-28.9696	77	91	(K)RCHEFVTFSCPGADK(G)	2Cys-am
1838.7785	1838.8745	-52.2048	321	336	(K)(M)
2065.0068	2064.9582	23.5600	376	392	(K)DVVIQDDDVECTMVEKR(V)	1Cys-am
2066.9431	2066.9374	2.7267	376	392	(K)DVVIQDDDVECTMVEKR(V)	1Met-ox
2073.1005	2073.0327	32.7095	179	197	(R)(L)
2076.0050	2075.9425	30.1204	142	158	(K)RCVMNVPSLCGTDHTER(R)	1Met-ox 2Cys-am
2076.0050	2075.9425	30.1204	143	159	(R)CVMNVPSLCGTDHTERR(G)	1Met-ox 2Cys-am
2082.9766	2083.0071	-14.6548	217	232	(K)CSLNPEWNETFRFQLK(E)	1Cys-am
2088.0648	2087.9108	73.7585	46	62	(K)(Q)
2097.8713	2098.0100	-66.0910	321	338	(K)(L)
2813.2829	2813.4004	-41.7946	1	27	(-)MADPAAGPPPSEGEESTVRFARKGALR(Q)	1Met-ox
2813.2829	2813.3317	-17.3590	217	238	(K)CSLNPEWNETFRFQLKESDKDR(R)	1Cys-am

The matched peptides cover 28% (193/673AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 25.6 Mean Error = -14.3507 ppm Data Tolerance = 84.1931 ppm Mean Missed Cleavages = 1.3