MS-Fit Search Results

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Sample ID (comment): HSG092
Database searched: SwissProt.1.27.2002
Molecular weight search (1000 - 100000 Da) selects 98142 entries.
Full pI range: 103988 entries.
Species search ( HUMAN MOUSE ) selects 12515 entries.
Combined molecular weight, pI and species searches select 11105 entries.
Pre searches select 11105 entries.
MS-Fit search selects 770 entries (results displayed for top 5 matches).

Parameters Used in Search

Considered modifications: | Oxidation of M | Protein N-terminus Acetylated | Acrylamide Modified Cys |

4	50.000 ppm	monoisotopic	Trypsin	2	carbamidomethylation	Hydrogen (H)	Free Acid (O H)
Min. # Peptides to Match	Peptide Mass Tolerance (+/-)	Peptide Masses are	Digest Used	Max. # Missed Cleavages	Cysteines Modified by	Peptide N terminus	Peptide C terminus

Result Summary

Rank	MOWSE Score	# (%) Masses Matched	Protein MW (Da)/pI	Species	Protein Name
1	6.21e+009	19/51 (37%)	32854.4 / 4.79	HUMAN	40S RIBOSOMAL PROTEIN SA (P40) (34/67 KDA LAMININ RECEPTOR) (COLON CARCINOMA LAMININ-BINDING PROTEIN) (NEM/1CHD4)
2	2.92e+007	15/51 (29%)	32719.2 / 4.74	MOUSE	40S ribosomal protein SA (P40) (34/67 kDa laminin receptor)
3	3.63e+003	7/51 (13%)	65163.8 / 6.07	HUMAN	COMPLEMENT COMPONENT C8 ALPHA CHAIN PRECURSOR
4	2.29e+003	11/51 (21%)	72374.6 / 8.99	HUMAN	ZINC FINGER PROTEIN 255 (BONE MARROW ZINC FINGER 2) (BMZF-2)
5	1.17e+003	5/51 (9%)	51131.0 / 8.76	MOUSE	Eukaryotic translation initiation factor 2 subunit 3, Y-linked (Eukaryotic translation initiation factor 2 gamma subunit, Y-linked) (eIF-2-gamma Y)

Detailed Results

1. 19/51 matches (37%). 32854.4 Da, pI = 4.79. Acc. # P08865. HUMAN. 40S RIBOSOMAL PROTEIN SA (P40) (34/67 KDA LAMININ RECEPTOR) (COLON CARCINOMA LAMININ-BINDING PROTEIN) (NEM/1CHD4).

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
656.4294	656.4459	-25.1814	58	63	(K)(A)
912.5336	912.5518	-20.0042	121	128	(R)(A)
1203.6626	1203.6486	11.6107	90	102	(K)(F)
1614.8193	1614.8037	9.6321	167	180	(K)(E)
1630.7922	1630.7986	-3.9285	167	180	(K)GAHSVGLMWWMLAR(E)	1Met-ox
1646.7719	1646.7936	-13.1621	167	180	(K)GAHSVGLMWWMLAR(E)	2Met-ox
1698.8681	1698.8604	4.5451	103	117	(R)(E)
1740.9453	1740.9496	-2.4908	64	80	(R)(N)
1816.9214	1816.9366	-8.4122	1	17	(-)SGALDVLQMKEEDVLK(F)	Acet N
1832.8646	1832.9316	-36.5066	1	17	(-)SGALDVLQMKEEDVLK(F)	1Met-ox Acet N
1881.8595	1881.8634	-2.0852	192	205	(R)(D)
1897.8562	1897.8583	-1.1305	192	205	(R)EHPWEVMPDLYFYR(D)	1Met-ox
2081.0760	2081.0568	9.2210	103	120	(R)(L)
2617.2685	2617.2761	-2.8963	18	40	(K)(R)
2633.3304	2633.2710	22.5587	18	40	(K)FLAAGTHLGGTNLDFQMEQYIYK(R)	1Met-ox
2722.2723	2722.2499	8.2090	192	212	(R)(E)
2738.2492	2738.2448	1.5978	192	212	(R)EHPWEVMPDLYFYRDPEEIEK(E)	1Met-ox
2773.2880	2773.3772	-32.1761	18	41	(K)(K)
3010.5442	3010.4944	16.5232	129	155	(R)ADHQPLTEASYVNLPTIALCNTDSPLR(Y)	1Cys-am

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The matched peptides cover 55% (165/295AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 37.3 Mean Error = -3.3724 ppm Data Tolerance = 32.1489 ppm Mean Missed Cleavages = 0.3

2. 15/51 matches (29%). 32719.2 Da, pI = 4.74. Acc. # P14206. MOUSE. 40S ribosomal protein SA (P40) (34/67 kDa laminin receptor).

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
656.4294 656.4459 -25.1814 58 63 (K)(A)
912.5336 912.5518 -20.0042 121 128 (R)(A)
1203.6626 1203.6486 11.6107 90 102 (K)(F)
1614.8193 1614.8037 9.6321 167 180 (K)(E)
1630.7922 1630.7986 -3.9285 167 180 (K)GAHSVGLMWWMLAR(E) 1Met-ox
1646.7719 1646.7936 -13.1621 167 180 (K)GAHSVGLMWWMLAR(E) 2Met-ox
1698.8681 1698.8604 4.5451 103 117 (R)(E)
1740.9453 1740.9496 -2.4908 64 80 (R)(N)
1816.9214 1816.9366 -8.4122 1 17 (-)SGALDVLQMKEEDVLK(L) Acet N
1832.8646 1832.9316 -36.5066 1 17 (-)SGALDVLQMKEEDVLK(L) 1Met-ox Acet N
1881.8595 1881.8634 -2.0852 192 205 (R)(D)
1897.8562 1897.8583 -1.1305 192 205 (R)EHPWEVMPDLYFYR(D) 1Met-ox
2081.0760 2081.0568 9.2210 103 120 (R)(L)
2722.2723 2722.2499 8.2090 192 212 (R)(E)
2738.2492 2738.2448 1.5978 192 212 (R)EHPWEVMPDLYFYRDPEEIEK(E) 1Met-ox

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The matched peptides cover 38% (114/295AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 29.4 Mean Error = -4.5391 ppm Data Tolerance = 27.9410 ppm Mean Missed Cleavages = 0.3

3. 7/51 matches (13%). 65163.8 Da, pI = 6.07. Acc. # P07357. HUMAN. COMPLEMENT COMPONENT C8 ALPHA CHAIN PRECURSOR.

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
842.5112 842.5073 4.6519 480 485 (K)(A)
1630.7922 1630.7358 34.6276 486 498 (R)(C)
1646.7719 1646.7307 25.0191 486 498 (R)ALDQYLMEFNACR(C) 1Met-ox
1720.8343 1720.8176 9.7332 521 537 (R)(A)
1816.9214 1816.8474 40.6791 485 498 (R)RALDQYLMEFNACR(C) 1Met-ox 1Cys-am
2617.2685 2617.3232 -20.9107 1 23 (-)(V)
2633.3304 2633.3181 4.6531 1 23 (-)MFAVVFFILSLMTCQPGVTAQEK(V) 1Met-ox

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m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
842.5112	842.5073	4.6519	480	485	(K)(A)
1630.7922	1630.7358	34.6276	486	498	(R)(C)
1646.7719	1646.7307	25.0191	486	498	(R)ALDQYLMEFNACR(C)	1Met-ox
1720.8343	1720.8176	9.7332	521	537	(R)(A)
1816.9214	1816.8474	40.6791	485	498	(R)RALDQYLMEFNACR(C)	1Met-ox 1Cys-am
2617.2685	2617.3232	-20.9107	1	23	(-)(V)
2633.3304	2633.3181	4.6531	1	23	(-)MFAVVFFILSLMTCQPGVTAQEK(V)	1Met-ox

The matched peptides cover 10% (59/584AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 13.7 Mean Error = 14.0647 ppm Data Tolerance = 42.1930 ppm Mean Missed Cleavages = 0.4

4. 11/51 matches (21%). 72374.6 Da, pI = 8.99. Acc. # Q9UID9. HUMAN. ZINC FINGER PROTEIN 255 (BONE MARROW ZINC FINGER 2) (BMZF-2).

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
1203.6626 1203.6196 35.6983 252 261 (R)(C)
1614.8193 1614.8539 -21.4059 380 392 (K)SFSRAPCLLKHER(L) 1Cys-am
1630.7922 1630.7252 41.0960 206 218 (K)CDICGKSFCGRSR(L) 2Cys-am
1646.7719 1646.7385 20.2444 324 336 (K)(S)
1652.8236 1652.8253 -0.9870 376 389 (K)(H)
1662.8167 1662.7732 26.1739 371 383 (K)LYNCKECGKSFSR(A) 1Cys-am
1702.8175 1702.8964 -46.3163 296 308 (K)SFRWASCLLKHQR(V) 1Cys-am
1720.8343 1720.8553 -12.1881 534 547 (R)HSRETPLKCEQHGK(N) 1Cys-am
1833.9231 1833.8628 32.9059 477 491 (R)LHSGEKPFKCEECGK(R) 2Cys-am
2233.0884 2232.9953 41.6922 222 239 (R)HSMVHTAEKPFRCDTCDK(S) 1Cys-am
2722.2723 2722.2830 -3.9310 161 183 (R)SALNVHHKLHTGEKPYNCEECGK(A) 1Cys-am

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m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
1203.6626	1203.6196	35.6983	252	261	(R)(C)
1614.8193	1614.8539	-21.4059	380	392	(K)SFSRAPCLLKHER(L)	1Cys-am
1630.7922	1630.7252	41.0960	206	218	(K)CDICGKSFCGRSR(L)	2Cys-am
1646.7719	1646.7385	20.2444	324	336	(K)(S)
1652.8236	1652.8253	-0.9870	376	389	(K)(H)
1662.8167	1662.7732	26.1739	371	383	(K)LYNCKECGKSFSR(A)	1Cys-am
1702.8175	1702.8964	-46.3163	296	308	(K)SFRWASCLLKHQR(V)	1Cys-am
1720.8343	1720.8553	-12.1881	534	547	(R)HSRETPLKCEQHGK(N)	1Cys-am
1833.9231	1833.8628	32.9059	477	491	(R)LHSGEKPFKCEECGK(R)	2Cys-am
2233.0884	2232.9953	41.6922	222	239	(R)HSMVHTAEKPFRCDTCDK(S)	1Cys-am
2722.2723	2722.2830	-3.9310	161	183	(R)SALNVHHKLHTGEKPYNCEECGK(A)	1Cys-am

The matched peptides cover 22% (141/623AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 21.6 Mean Error = 10.2711 ppm Data Tolerance = 58.2653 ppm Mean Missed Cleavages = 1.4

5. 5/51 matches (9%). 51131.0 Da, pI = 8.76. Acc. # Q9Z0N2. MOUSE. Eukaryotic translation initiation factor 2 subunit 3, Y-linked (Eukaryotic translation initiation factor 2 gamma subunit, Y-linked) (eIF-2-gamma Y).

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
1646.7719 1646.8536 -49.6052 401 416 (K)(V)
1662.8167 1662.8485 -19.0951 401 416 (K)NEVLMVNIGSLSTGGR(V) 1Met-ox
1736.8025 1736.8165 -8.0552 261 275 (R)SFDVNKPGCEVDDLK(G) 1Cys-am
1881.8595 1881.8176 22.2362 104 121 (R)(G)
2738.2492 2738.3208 -26.1414 104 128 (R)SCGSSTPDEFPSDIPGTKGNFRLVR(H) 1Cys-am

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m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
1646.7719	1646.8536	-49.6052	401	416	(K)(V)
1662.8167	1662.8485	-19.0951	401	416	(K)NEVLMVNIGSLSTGGR(V)	1Met-ox
1736.8025	1736.8165	-8.0552	261	275	(R)SFDVNKPGCEVDDLK(G)	1Cys-am
1881.8595	1881.8176	22.2362	104	121	(R)(G)
2738.2492	2738.3208	-26.1414	104	128	(R)SCGSSTPDEFPSDIPGTKGNFRLVR(H)	1Cys-am

The matched peptides cover 11% (56/472AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 9.8 Mean Error = -16.1321 ppm Data Tolerance = 52.6002 ppm Mean Missed Cleavages = 0.4