MS-Fit Search Results

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Sample ID (comment): HSG093
Database searched: SwissProt.1.27.2002
Molecular weight search (1000 - 100000 Da) selects 98142 entries.
Full pI range: 103988 entries.
Species search ( HUMAN MOUSE ) selects 12515 entries.
Combined molecular weight, pI and species searches select 11105 entries.
Pre searches select 11105 entries.
MS-Fit search selects 1183 entries (results displayed for top 5 matches).

Parameters Used in Search

Considered modifications: | Oxidation of M | Protein N-terminus Acetylated | Acrylamide Modified Cys |

4	50.000 ppm	monoisotopic	Trypsin	2	carbamidomethylation	Hydrogen (H)	Free Acid (O H)
Min. # Peptides to Match	Peptide Mass Tolerance (+/-)	Peptide Masses are	Digest Used	Max. # Missed Cleavages	Cysteines Modified by	Peptide N terminus	Peptide C terminus

Result Summary

Rank	MOWSE Score	# (%) Masses Matched	Protein MW (Da)/pI	Species	Protein Name
1	2.1e+014	33/54 (61%)	57488.7 / 6.01	HUMAN	T-complex protein 1, beta subunit (TCP-1-beta) (CCT-beta)
2	9.47e+010	25/54 (46%)	57447.7 / 5.97	MOUSE	T-COMPLEX PROTEIN 1, BETA SUBUNIT (TCP-1-BETA) (CCT-BETA)
3	6.05e+004	11/54 (20%)	88997.7 / 6.29	HUMAN	Diacylglycerol kinase, gamma (Diglyceride kinase) (DGK-gamma) (DAG kinase gamma)
4	4.6e+003	5/54 (9%)	49974.0 / 4.71	HUMAN	Placental ribonuclease inhibitor (Ribonuclease/angiogenin inhibitor) (RAI) (RI)
5	4.48e+003	9/54 (16%)	97623.2 / 5.56	HUMAN	Coatomer gamma-2 subunit (Gamma-2 coat protein) (Gamma-2 COP)

Detailed Results

1. 33/54 matches (61%). 57488.7 Da, pI = 6.01. Acc. # P78371. HUMAN. T-complex protein 1, beta subunit (TCP-1-beta) (CCT-beta).

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
745.4349	745.4572	-29.9001	51	57	(K)(D)
870.5175	870.5413	-27.3637	182	189	(K)(L)
1130.5269	1130.5529	-23.0270	285	293	(K)(Q)
1144.5579	1144.5686	-9.3016	285	293	(K)HGINCFINR(Q)	1Cys-am
1291.7094	1291.7275	-14.0276	121	131	(K)(E)
1291.7094	1291.6568	40.7366	237	248	(K)(I)
1291.7094	1291.6455	49.4340	348	358	(K)LIEEVMIGEDK(L)	1Met-ox
1301.7480	1301.7469	0.8305	1	13	(-)ASLSLAPVNIFK(A)	Acet N
1307.7122	1307.6517	46.2930	237	248	(K)ILIANTGMDTDK(I)	1Met-ox
1330.6456	1330.6603	-11.0710	377	388	(R)(S)
1419.8095	1419.8225	-9.1370	120	131	(K)(E)
1435.8161	1435.8021	9.7532	251	263	(K)(V)
1517.8767	1517.8943	-11.6262	26	40	(R)(S)
1530.6764	1530.6971	-13.5114	467	481	(R)(E)
1568.8437	1568.8470	-2.1446	389	402	(R)SLHDALCVLAQTVK(D)	1Cys-am
1582.9006	1582.9168	-10.2411	502	516	(R)(V)
1739.0105	1739.0179	-4.2689	501	516	(K)(V)
1781.8350	1781.9220	-48.8430	155	170	(K)(L)
1956.0687	1956.0741	-2.7578	359	376	(K)(G)
1970.0717	1970.0897	-9.1491	359	376	(K)LIHFSGVALGEACTIVLR(G)	1Cys-am
2008.9988	2009.0265	-13.7724	482	500	(R)(R)
2025.0387	2025.0214	8.5467	482	500	(R)EGTIGDMAILGITESFQVK(R)	1Met-ox
2041.0630	2041.0494	6.6691	204	222	(K)(K)
2041.0630	2041.0494	6.6691	205	223	(K)(I)
2097.1283	2097.1232	2.4440	323	342	(R)(L)
2169.1279	2169.1443	-7.5837	204	223	(K)(I)
2228.2269	2228.2668	-17.9039	171	189	(K)(L)
2265.3182	2265.3182	0.0185	502	522	(R)(A)
2288.1754	2288.1622	5.7645	90	111	(R)(E)
2347.2017	2347.1968	2.1143	445	466	(R)(A)
2363.1980	2363.1917	2.6612	445	466	(R)MLPTIIADNAGYDSADLVAQLR(A)	1Met-ox
2380.1361	2380.1212	6.2777	406	427	(R)TVYGGGCSEMLMAHAVTQLANR(T)	1Cys-am
2396.0775	2396.1161	-16.1202	406	427	(R)TVYGGGCSEMLMAHAVTQLANR(T)	1Met-ox 1Cys-am
2412.0908	2412.1110	-8.3784	406	427	(R)TVYGGGCSEMLMAHAVTQLANR(T)	2Met-ox 1Cys-am
3209.5887	3209.5730	4.9073	294	322	(R)(L)
3225.6771	3225.5679	33.8368	294	322	(R)QLIYNYPEQLFGAAGVMAIEHADFAGVER(L)	1Met-ox

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Click individual mass to do a non-specific cleavage search.

The matched peptides cover 67% (362/535AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 61.1 Mean Error = -1.7548 ppm Data Tolerance = 40.1923 ppm Mean Missed Cleavages = 0.3

2. 25/54 matches (46%). 57447.7 Da, pI = 5.97. Acc. # P80314. MOUSE. T-COMPLEX PROTEIN 1, BETA SUBUNIT (TCP-1-BETA) (CCT-BETA).

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
745.4349 745.4572 -29.9001 51 57 (K)(D)
1130.5269 1130.5529 -23.0270 285 293 (K)(Q)
1144.5579 1144.5686 -9.3016 285 293 (K)HGINCFINR(Q) 1Cys-am
1291.7094 1291.6568 40.7366 237 248 (K)(I)
1291.7094 1291.6455 49.4340 348 358 (K)LIEEVMIGEDK(L) 1Met-ox
1301.7480 1301.7469 0.8305 1 13 (-)ASLSLAPVNIFK(A) Acet N
1307.7122 1307.7224 -7.8017 121 131 (K)(E)
1307.7122 1307.6517 46.2930 237 248 (K)ILIANTGMDTDK(I) 1Met-ox
1330.6456 1330.6603 -11.0710 377 388 (R)(S)
1435.8161 1435.8174 -0.8723 120 131 (K)(E)
1435.8161 1435.8021 9.7532 251 263 (K)(V)
1568.8437 1568.8470 -2.1446 389 402 (R)SLHDALCVLAQTVK(D) 1Cys-am
1582.9006 1582.9168 -10.2411 502 516 (R)(V)
1739.0105 1739.0179 -4.2689 501 516 (K)(V)
1956.0687 1956.0741 -2.7578 359 376 (K)(G)
1970.0717 1970.0897 -9.1491 359 376 (K)LIHFSGVALGEACTIVLR(G) 1Cys-am
2041.0630 2041.0494 6.6691 204 222 (K)(K)
2041.0630 2041.0494 6.6691 205 223 (K)(I)
2097.1283 2097.1232 2.4440 323 342 (R)(L)
2169.1279 2169.1443 -7.5837 204 223 (K)(I)
2265.3182 2265.3182 0.0185 502 522 (R)(A)
2288.1754 2288.1622 5.7645 90 111 (R)(E)
2347.2017 2347.1968 2.1143 445 466 (R)(A)
2363.1980 2363.1917 2.6612 445 466 (R)MLPTIIADNAGYDSADLVAQLR(A) 1Met-ox
2380.1361 2380.1212 6.2777 406 427 (R)TVYGGGCSEMLMAHAVTQLANR(T) 1Cys-am
2396.0775 2396.1161 -16.1202 406 427 (R)TVYGGGCSEMLMAHAVTQLANR(T) 1Met-ox 1Cys-am
2412.0908 2412.1110 -8.3784 406 427 (R)TVYGGGCSEMLMAHAVTQLANR(T) 2Met-ox 1Cys-am
3209.5887 3209.5730 4.9073 294 322 (R)(L)
3225.6771 3225.5679 33.8368 294 322 (R)QLIYNYPEQLFGAAGVMAIEHADFAGVER(L) 1Met-ox

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The matched peptides cover 51% (278/535AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 46.3 Mean Error = 2.6135 ppm Data Tolerance = 37.4044 ppm Mean Missed Cleavages = 0.3

3. 11/54 matches (20%). 88997.7 Da, pI = 6.29. Acc. # P49619. HUMAN. Diacylglycerol kinase, gamma (Diglyceride kinase) (DGK-gamma) (DAG kinase gamma).

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
1565.8638 1565.8328 19.8113 531 545 (R)(D)
2008.9988 2009.0642 -32.5485 528 545 (R)CLRWGGGYEGGSLTKILK(D) 1Cys-am
2041.0630 2040.9644 48.2933 281 296 (K)KPTYCNFCHIMLMGVR(K) 1Cys-am
2169.1279 2169.0594 31.5818 281 297 (K)KPTYCNFCHIMLMGVRK(Q) 1Cys-am
2211.0998 2211.0374 28.2446 354 370 (K)(M)
2225.1422 2225.0530 40.0664 354 370 (K)SIKCYQSVTARHCVWCR(M) 1Cys-am
2233.0827 2233.0777 2.2390 1 19 (-)GEERWVSLTPEEFDQLQK(Y) Acet N
2285.1980 2285.1065 40.0490 43 60 (K)(A)
2303.1833 2303.0697 49.3359 743 761 (K)(I)
2347.2017 2347.0959 45.1014 743 761 (K)LLPMQVDGEPWMQPCCTIK(I) 1Met-ox 2Cys-am
2363.1980 2363.0908 45.3574 743 761 (K)LLPMQVDGEPWMQPCCTIK(I) 2Met-ox 2Cys-am

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Click individual mass to do a non-specific cleavage search.

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
1565.8638	1565.8328	19.8113	531	545	(R)(D)
2008.9988	2009.0642	-32.5485	528	545	(R)CLRWGGGYEGGSLTKILK(D)	1Cys-am
2041.0630	2040.9644	48.2933	281	296	(K)KPTYCNFCHIMLMGVR(K)	1Cys-am
2169.1279	2169.0594	31.5818	281	297	(K)KPTYCNFCHIMLMGVRK(Q)	1Cys-am
2211.0998	2211.0374	28.2446	354	370	(K)(M)
2225.1422	2225.0530	40.0664	354	370	(K)SIKCYQSVTARHCVWCR(M)	1Cys-am
2233.0827	2233.0777	2.2390	1	19	(-)GEERWVSLTPEEFDQLQK(Y)	Acet N
2285.1980	2285.1065	40.0490	43	60	(K)(A)
2303.1833	2303.0697	49.3359	743	761	(K)(I)
2347.2017	2347.0959	45.1014	743	761	(K)LLPMQVDGEPWMQPCCTIK(I)	1Met-ox 2Cys-am
2363.1980	2363.0908	45.3574	743	761	(K)LLPMQVDGEPWMQPCCTIK(I)	2Met-ox 2Cys-am

The matched peptides cover 13% (108/791AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 20.4 Mean Error = 28.8665 ppm Data Tolerance = 49.6104 ppm Mean Missed Cleavages = 0.9

4. 5/54 matches (9%). 49974.0 Da, pI = 4.71. Acc. # P13489. HUMAN. Placental ribonuclease inhibitor (Ribonuclease/angiogenin inhibitor) (RAI) (RI).

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
1530.6764 1530.7400 -41.5437 288 302 (K)(L)
2008.9988 2008.9948 1.9951 239 257 (K)(L)
2025.0387 2024.9898 24.1900 239 257 (K)LGDVGMAELCPGLLHPSSR(L) 1Met-ox
2288.1754 2288.1671 3.6269 303 321 (R)(S)
2412.0908 2412.1652 -30.8244 65 86 (R)SNELGDVGVHCVLQGLQTPSCK(I) 1Cys-am

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m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
1530.6764	1530.7400	-41.5437	288	302	(K)(L)
2008.9988	2008.9948	1.9951	239	257	(K)(L)
2025.0387	2024.9898	24.1900	239	257	(K)LGDVGMAELCPGLLHPSSR(L)	1Met-ox
2288.1754	2288.1671	3.6269	303	321	(R)(S)
2412.0908	2412.1652	-30.8244	65	86	(R)SNELGDVGVHCVLQGLQTPSCK(I)	1Cys-am

The matched peptides cover 16% (75/461AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 9.3 Mean Error = -8.5112 ppm Data Tolerance = 54.0010 ppm Mean Missed Cleavages = 0.0

5. 9/54 matches (16%). 97623.2 Da, pI = 5.56. Acc. # Q9UBF2. HUMAN. Coatomer gamma-2 subunit (Gamma-2 coat protein) (Gamma-2 COP).

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
1781.8350 1781.8777 -24.0120 152 168 (K)VSSVSSSALVSSLHMMK(I) 2Met-ox
2211.0998 2211.1998 -45.2163 25 43 (K)(C)
2283.2136 2283.2535 -17.4586 284 304 (R)(Y)
2284.1970 2284.1616 15.4816 127 145 (R)ALCRITDGTMLQAIERYMK(Q) 1Cys-am
2303.1833 2303.1640 8.3601 76 93 (R)LFQSNDQTLRRMCYLTIK(E) 1Met-ox
2335.1884 2335.1426 19.6227 793 812 (K)(S)
2396.0775 2396.1855 -45.0826 263 283 (K)(E)
2412.0908 2412.1804 -37.1489 263 283 (K)HEMVIYEAASAIIHLPNCTAR(E) 1Met-ox
3225.6771 3225.6360 12.7251 310 338 (R)(S)

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m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
1781.8350	1781.8777	-24.0120	152	168	(K)VSSVSSSALVSSLHMMK(I)	2Met-ox
2211.0998	2211.1998	-45.2163	25	43	(K)(C)
2283.2136	2283.2535	-17.4586	284	304	(R)(Y)
2284.1970	2284.1616	15.4816	127	145	(R)ALCRITDGTMLQAIERYMK(Q)	1Cys-am
2303.1833	2303.1640	8.3601	76	93	(R)LFQSNDQTLRRMCYLTIK(E)	1Met-ox
2335.1884	2335.1426	19.6227	793	812	(K)(S)
2396.0775	2396.1855	-45.0826	263	283	(K)(E)
2412.0908	2412.1804	-37.1489	263	283	(K)HEMVIYEAASAIIHLPNCTAR(E)	1Met-ox
3225.6771	3225.6360	12.7251	310	338	(R)(S)

The matched peptides cover 18% (164/871AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 16.7 Mean Error = -12.5254 ppm Data Tolerance = 53.7724 ppm Mean Missed Cleavages = 0.9