MS-Fit Search Results

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Sample ID (comment): HSG094
Database searched: SwissProt.1.27.2002
Molecular weight search (1000 - 100000 Da) selects 98142 entries.
Full pI range: 103988 entries.
Species search ( HUMAN MOUSE ) selects 12515 entries.
Combined molecular weight, pI and species searches select 11105 entries.
Pre searches select 11105 entries.
MS-Fit search selects 3232 entries (results displayed for top 5 matches).

Parameters Used in Search

Considered modifications: | Oxidation of M | Protein N-terminus Acetylated | Acrylamide Modified Cys |

4	75.000 ppm	monoisotopic	Trypsin	2	carbamidomethylation	Hydrogen (H)	Free Acid (O H)
Min. # Peptides to Match	Peptide Mass Tolerance (+/-)	Peptide Masses are	Digest Used	Max. # Missed Cleavages	Cysteines Modified by	Peptide N terminus	Peptide C terminus

Result Summary

Rank	MOWSE Score	# (%) Masses Matched	Protein MW (Da)/pI	Species	Protein Name
1	1.18e+009	23/78 (29%)	60331.2 / 6.23	HUMAN	T-COMPLEX PROTEIN 1, GAMMA SUBUNIT (TCP-1-GAMMA) (CCT-GAMMA)
2	6.49e+008	22/78 (28%)	60630.6 / 6.28	MOUSE	T-COMPLEX PROTEIN 1, GAMMA SUBUNIT (TCP-1-GAMMA) (CCT-GAMMA) (MATRICIN)
3	4.89e+007	19/78 (24%)	66018.2 / 8.16	HUMAN	Keratin, type II cytoskeletal 1 (Cytokeratin 1) (K1) (CK 1) (67 kDa cytokeratin) (Hair alpha protein)
4	1.05e+005	11/78 (14%)	59519.1 / 5.13	HUMAN	Keratin, type I cytoskeletal 10 (Cytokeratin 10) (K10) (CK 10)
5	2.37e+004	14/78 (17%)	51050.6 / 5.32	HUMAN	Keratin, type I cytoskeletal 16 (Cytokeratin 16) (K16) (CK 16)

Detailed Results

1. 23/78 matches (29%). 60331.2 Da, pI = 6.23. Acc. # P49368. HUMAN. T-COMPLEX PROTEIN 1, GAMMA SUBUNIT (TCP-1-GAMMA) (CCT-GAMMA).

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
677.3212	677.3656	-65.6367	216	221	(R)GVMINK(D)	1Met-ox
677.3212	677.2855	52.7171	529	534	(K)(Q)
702.4546	702.4514	4.5561	461	466	(R)(A)
860.5126	860.5028	11.3803	370	376	(K)ACTILLR(G)	1Cys-am
1109.5264	1109.5050	19.3545	181	189	(K)(K)
1166.7073	1166.6897	15.0740	438	448	(R)(T)
1185.7589	1185.7207	32.2259	507	517	(K)(I)
1189.6936	1189.6727	17.5695	370	380	(K)(E)
1234.7186	1234.6717	37.9607	127	137	(R)(K)
1234.7186	1234.6717	37.9607	128	138	(K)(I)
1248.6517	1248.5829	55.0634	389	398	(R)NLQDAMQVCR(N)	1Cys-am
1254.7238	1254.7098	11.1388	491	501	(K)(L)
1337.7126	1337.6312	60.8178	427	437	(K)(A)
1347.7677	1347.7055	46.1779	449	460	(R)TLIQNCGASTIR(L)	1Cys-am
1353.6794	1353.6261	39.3419	427	437	(K)AMTGVEQWPYR(A)	1Met-ox
1403.7548	1403.7105	31.5221	294	305	(K)(A)
1407.7901	1407.8099	-14.0389	237	248	(R)(G)
1416.7751	1416.7222	37.3226	339	352	(R)(K)
1442.8241	1442.7677	39.0827	203	215	(K)IPGGIIEDSCVLR(G)	1Cys-am
1561.8528	1561.8048	30.7353	167	180	(R)WSSLACNIALDAVK(M)	1Cys-am
1784.9611	1784.9580	1.6893	200	215	(R)(G)
1798.9997	1798.9737	14.4612	200	215	(R)VEKIPGGIIEDSCVLR(G)	1Cys-am
1911.9910	1912.0980	-55.9752	20	37	(R)(T)
1963.0484	1962.9159	67.4914	354	369	(K)IGDEYFTFITDCKDPK(A)	1Cys-am
2496.4286	2496.3310	39.1051	330	352	(R)(K)

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The matched peptides cover 41% (227/544AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 29.5 Mean Error = 22.6839 ppm Data Tolerance = 63.5413 ppm Mean Missed Cleavages = 0.4

2. 22/78 matches (28%). 60630.6 Da, pI = 6.28. Acc. # P80318. MOUSE. T-COMPLEX PROTEIN 1, GAMMA SUBUNIT (TCP-1-GAMMA) (CCT-GAMMA) (MATRICIN).

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
677.3212 677.3656 -65.6367 217 222 (R)GVMINK(D) 1Met-ox
702.4546 702.4514 4.5561 462 467 (R)(A)
860.5126 860.5028 11.3803 371 377 (K)ACTILLR(G) 1Cys-am
1166.7073 1166.6897 15.0740 439 449 (R)(T)
1185.7589 1185.7207 32.2259 508 518 (K)(I)
1189.6936 1189.6727 17.5695 371 381 (K)(E)
1248.6517 1248.6072 35.6137 250 260 (K)(E)
1248.6517 1248.5829 55.0634 390 399 (R)NLQDAMQVCR(N) 1Cys-am
1254.7238 1254.7098 11.1388 492 502 (K)(L)
1337.7126 1337.6312 60.8178 428 438 (K)(A)
1347.7677 1347.7055 46.1779 450 461 (R)TLIQNCGASTIR(L) 1Cys-am
1353.6794 1353.6261 39.3419 428 438 (K)AMTGVEQWPYR(A) 1Met-ox
1403.7548 1403.7105 31.5221 295 306 (K)(A)
1407.7901 1407.8099 -14.0389 238 249 (R)(G)
1416.7751 1416.7222 37.3226 340 353 (R)(K)
1442.8241 1442.7677 39.0827 204 216 (K)IPGGIIEDSCVLR(G) 1Cys-am
1561.8528 1561.8048 30.7353 168 181 (R)WSSLACNIALDAVK(T) 1Cys-am
1784.9611 1784.9580 1.6893 201 216 (R)(G)
1798.9997 1798.9737 14.4612 201 216 (R)VEKIPGGIIEDSCVLR(G) 1Cys-am
1911.9910 1912.0980 -55.9752 21 38 (R)(T)
1963.0484 1962.9159 67.4914 355 370 (K)IGDEYFTFITDCKDPK(A) 1Cys-am
2154.1434 2154.0315 51.9509 249 266 (K)(I)
2496.4286 2496.3310 39.1051 331 353 (R)(K)

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Click individual mass to do a non-specific cleavage search.

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
677.3212	677.3656	-65.6367	217	222	(R)GVMINK(D)	1Met-ox
702.4546	702.4514	4.5561	462	467	(R)(A)
860.5126	860.5028	11.3803	371	377	(K)ACTILLR(G)	1Cys-am
1166.7073	1166.6897	15.0740	439	449	(R)(T)
1185.7589	1185.7207	32.2259	508	518	(K)(I)
1189.6936	1189.6727	17.5695	371	381	(K)(E)
1248.6517	1248.6072	35.6137	250	260	(K)(E)
1248.6517	1248.5829	55.0634	390	399	(R)NLQDAMQVCR(N)	1Cys-am
1254.7238	1254.7098	11.1388	492	502	(K)(L)
1337.7126	1337.6312	60.8178	428	438	(K)(A)
1347.7677	1347.7055	46.1779	450	461	(R)TLIQNCGASTIR(L)	1Cys-am
1353.6794	1353.6261	39.3419	428	438	(K)AMTGVEQWPYR(A)	1Met-ox
1403.7548	1403.7105	31.5221	295	306	(K)(A)
1407.7901	1407.8099	-14.0389	238	249	(R)(G)
1416.7751	1416.7222	37.3226	340	353	(R)(K)
1442.8241	1442.7677	39.0827	204	216	(K)IPGGIIEDSCVLR(G)	1Cys-am
1561.8528	1561.8048	30.7353	168	181	(R)WSSLACNIALDAVK(T)	1Cys-am
1784.9611	1784.9580	1.6893	201	216	(R)(G)
1798.9997	1798.9737	14.4612	201	216	(R)VEKIPGGIIEDSCVLR(G)	1Cys-am
1911.9910	1912.0980	-55.9752	21	38	(R)(T)
1963.0484	1962.9159	67.4914	355	370	(K)IGDEYFTFITDCKDPK(A)	1Cys-am
2154.1434	2154.0315	51.9509	249	266	(K)(I)
2496.4286	2496.3310	39.1051	331	353	(R)(K)

The matched peptides cover 39% (217/545AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 28.2 Mean Error = 22.0291 ppm Data Tolerance = 65.8635 ppm Mean Missed Cleavages = 0.4

3. 19/78 matches (24%). 66018.2 Da, pI = 8.16. Acc. # P04264. HUMAN. Keratin, type II cytoskeletal 1 (Cytokeratin 1) (K1) (CK 1) (67 kDa cytokeratin) (Hair alpha protein).

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
670.3421 670.3273 22.1121 387 392 (R)(N)
832.4961 832.4892 8.2864 75 82 (K)(G)
973.5736 973.5318 42.9347 396 403 (K)(V)
1045.6227 1045.5754 45.1810 178 185 (K)(S)
1179.6593 1179.6010 49.4041 377 386 (K)(H)
1277.7403 1277.7105 23.2851 473 483 (K)(T)
1302.7209 1302.7157 3.9929 344 355 (R)(A)
1302.7209 1302.7018 14.6775 393 403 (R)(V)
1308.6872 1308.6548 24.7564 268 277 (R)(T)
1421.7611 1421.7389 15.6482 277 288 (K)(K)
1475.8243 1475.7858 26.0535 200 211 (R)(W)
1475.8243 1475.7494 50.7098 212 223 (K)(T)
1549.7810 1549.8338 -34.0633 277 289 (K)(D)
1638.8639 1638.8604 2.1335 186 199 (K)(F)
1657.8589 1657.7934 39.4759 13 29 (R)(R)
1716.9123 1716.8517 35.3238 418 432 (K)(G)
1765.7965 1765.7353 34.7032 46 65 (R)(S)
1797.9622 1797.9057 31.4173 289 304 (K)KDVDGAYMTKVDLQAK(L) 1Met-ox
1798.9997 1799.0139 -7.8988 393 407 (R)(L)
1994.0613 1993.9772 42.1754 224 239 (R)(R)
2384.0439 2383.9524 38.3967 519 549 (R)(G)

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Click individual mass to do a non-specific cleavage search.

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
670.3421	670.3273	22.1121	387	392	(R)(N)
832.4961	832.4892	8.2864	75	82	(K)(G)
973.5736	973.5318	42.9347	396	403	(K)(V)
1045.6227	1045.5754	45.1810	178	185	(K)(S)
1179.6593	1179.6010	49.4041	377	386	(K)(H)
1277.7403	1277.7105	23.2851	473	483	(K)(T)
1302.7209	1302.7157	3.9929	344	355	(R)(A)
1302.7209	1302.7018	14.6775	393	403	(R)(V)
1308.6872	1308.6548	24.7564	268	277	(R)(T)
1421.7611	1421.7389	15.6482	277	288	(K)(K)
1475.8243	1475.7858	26.0535	200	211	(R)(W)
1475.8243	1475.7494	50.7098	212	223	(K)(T)
1549.7810	1549.8338	-34.0633	277	289	(K)(D)
1638.8639	1638.8604	2.1335	186	199	(K)(F)
1657.8589	1657.7934	39.4759	13	29	(R)(R)
1716.9123	1716.8517	35.3238	418	432	(K)(G)
1765.7965	1765.7353	34.7032	46	65	(R)(S)
1797.9622	1797.9057	31.4173	289	304	(K)KDVDGAYMTKVDLQAK(L)	1Met-ox
1798.9997	1799.0139	-7.8988	393	407	(R)(L)
1994.0613	1993.9772	42.1754	224	239	(R)(R)
2384.0439	2383.9524	38.3967	519	549	(R)(G)

The matched peptides cover 37% (244/644AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 24.4 Mean Error = 24.2241 ppm Data Tolerance = 42.2363 ppm Mean Missed Cleavages = 0.6

4. 11/78 matches (14%). 59519.1 Da, pI = 5.13. Acc. # P13645. HUMAN. Keratin, type I cytoskeletal 10 (Cytokeratin 10) (K10) (CK 10).

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
807.4069 807.4001 8.3957 229 235 (R)(L)
1090.5749 1090.5315 39.7530 148 156 (K)(L)
1109.5264 1109.4904 32.5178 335 343 (K)(S)
1234.7186 1234.6796 31.5923 236 245 (R)(Q)
1434.7906 1434.7705 14.0023 440 450 (K)(S)
1493.7978 1493.7348 42.1235 323 334 (R)(D)
1549.7810 1549.6777 66.6574 16 35 (R)(I)
1707.8393 1707.7727 39.0165 41 59 (K)(G)
2083.1105 2082.9654 69.6615 423 439 (R)(I)
2176.0836 2176.0998 -7.4461 238 256 (K)(R)
2872.5394 2872.3933 50.8668 296 322 (R)(S)

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Click individual mass to do a non-specific cleavage search.

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)
807.4069	807.4001	8.3957	229	235	(R)(L)
1090.5749	1090.5315	39.7530	148	156	(K)(L)
1109.5264	1109.4904	32.5178	335	343	(K)(S)
1234.7186	1234.6796	31.5923	236	245	(R)(Q)
1434.7906	1434.7705	14.0023	440	450	(K)(S)
1493.7978	1493.7348	42.1235	323	334	(R)(D)
1549.7810	1549.6777	66.6574	16	35	(R)(I)
1707.8393	1707.7727	39.0165	41	59	(K)(G)
2083.1105	2082.9654	69.6615	423	439	(R)(I)
2176.0836	2176.0998	-7.4461	238	256	(K)(R)
2872.5394	2872.3933	50.8668	296	322	(R)(S)

The matched peptides cover 25% (152/593AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 14.1 Mean Error = 35.1946 ppm Data Tolerance = 46.9577 ppm Mean Missed Cleavages = 0.4

5. 14/78 matches (17%). 51050.6 Da, pI = 5.32. Acc. # P30654. HUMAN. Keratin, type I cytoskeletal 16 (Cytokeratin 16) (K16) (CK 16).

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
807.4069 807.4001 8.3957 198 204 (R)(T)
860.5126 860.5205 -9.2569 313 319 (K)(V)
1029.6030 1029.5944 8.3577 227 235 (R)(T)
1036.5750 1036.5468 27.2949 304 312 (R)(T)
1090.5749 1090.5315 39.7530 120 128 (K)(L)
1353.6794 1353.5857 69.1958 1 12 (-)STCSHQFTSSR(S) 1Cys-am Acet N
1377.7077 1377.7338 -18.9594 319 331 (K)(S)
1379.7723 1379.7283 31.9217 409 419 (K)(R)
1657.8589 1657.7896 41.7922 164 176 (K)DCSPYFKTIEDLR(H) 1Cys-am
1758.8925 1758.7903 58.0965 1 15 (-)MSTCSHQFTSSRSTK(G) 1Cys-am
1768.9240 1768.8692 30.9805 157 170 (R)QRPSEIKDCSPYFK(T) 1Cys-am
2064.2384 2064.1453 45.0908 179 197 (K)(L)
2176.0836 2176.1573 -33.8755 320 339 (K)(V)
2496.4286 2496.2557 69.2608 157 176 (R)QRPSEIKDCSPYFKTIEDLR(H) 1Cys-am

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Click individual mass to do a non-specific cleavage search.

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
807.4069	807.4001	8.3957	198	204	(R)(T)
860.5126	860.5205	-9.2569	313	319	(K)(V)
1029.6030	1029.5944	8.3577	227	235	(R)(T)
1036.5750	1036.5468	27.2949	304	312	(R)(T)
1090.5749	1090.5315	39.7530	120	128	(K)(L)
1353.6794	1353.5857	69.1958	1	12	(-)STCSHQFTSSR(S)	1Cys-am Acet N
1377.7077	1377.7338	-18.9594	319	331	(K)(S)
1379.7723	1379.7283	31.9217	409	419	(K)(R)
1657.8589	1657.7896	41.7922	164	176	(K)DCSPYFKTIEDLR(H)	1Cys-am
1758.8925	1758.7903	58.0965	1	15	(-)MSTCSHQFTSSRSTK(G)	1Cys-am
1768.9240	1768.8692	30.9805	157	170	(R)QRPSEIKDCSPYFK(T)	1Cys-am
2064.2384	2064.1453	45.0908	179	197	(K)(L)
2176.0836	2176.1573	-33.8755	320	339	(K)(V)
2496.4286	2496.2557	69.2608	157	176	(R)QRPSEIKDCSPYFKTIEDLR(H)	1Cys-am

The matched peptides cover 26% (126/474AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 17.9 Mean Error = 26.2891 ppm Data Tolerance = 63.4921 ppm Mean Missed Cleavages = 0.7