MS-Fit Search Results

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Sample ID (comment): HSG095
Database searched: SwissProt.1.27.2002
Molecular weight search (1000 - 100000 Da) selects 98142 entries.
Full pI range: 103988 entries.
Species search ( HUMAN MOUSE ) selects 12515 entries.
Combined molecular weight, pI and species searches select 11105 entries.
Pre searches select 11105 entries.
MS-Fit search selects 991 entries (results displayed for top 5 matches).

Parameters Used in Search

Considered modifications: | Oxidation of M | Protein N-terminus Acetylated | Acrylamide Modified Cys |

4	50.000 ppm	monoisotopic	Trypsin	2	carbamidomethylation	Hydrogen (H)	Free Acid (O H)
Min. # Peptides to Match	Peptide Mass Tolerance (+/-)	Peptide Masses are	Digest Used	Max. # Missed Cleavages	Cysteines Modified by	Peptide N terminus	Peptide C terminus

Result Summary

Rank	MOWSE Score	# (%) Masses Matched	Protein MW (Da)/pI	Species	Protein Name
1	5.75e+012	24/51 (47%)	56158.1 / 7.13	HUMAN	Succinyl-CoA:3-ketoacid-coenzyme A transferase, mitochondrial precursor (Succinyl CoA:3-oxoacid CoA-transferase)
2	6.49e+004	15/51 (29%)	82437.2 / 9.14	HUMAN	ZINC FINGER PROTEIN 225
3	7.27e+003	11/51 (21%)	73052.2 / 9.33	HUMAN	RAF proto-oncogene serine/threonine-protein kinase (RAF-1) (C-RAF)
4	6.92e+003	9/51 (17%)	71389.5 / 8.88	HUMAN	ZINC FINGER PROTEIN 221
5	3.1e+003	8/51 (15%)	79223.9 / 8.51	MOUSE	LONG-CHAIN-FATTY-ACID--COA LIGASE 4 (LONG-CHAIN ACYL-COA SYNTHETASE 4) (LACS 4) (MACS4)

Detailed Results

1. 24/51 matches (47%). 56158.1 Da, pI = 7.13. Acc. # P55809. HUMAN. Succinyl-CoA:3-ketoacid-coenzyme A transferase, mitochondrial precursor (Succinyl CoA:3-oxoacid CoA-transferase).

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
776.4316	776.4419	-13.2611	218	224	(R)(K)
1037.4878	1037.4912	-3.2735	229	236	(R)NFNLPMCK(A)	1Cys-am
1045.5637	1045.5174	44.1925	512	520	(K)(-)
1053.4765	1053.4861	-9.1552	229	236	(R)NFNLPMCK(A)	1Met-ox 1Cys-am
1100.5933	1100.6064	-11.8622	177	187	(K)(E)
1100.5933	1100.5774	14.4799	437	446	(K)(G)
1168.5876	1168.5890	-1.2342	42	51	(K)(D)
1168.5876	1168.5308	48.5435	501	511	(K)(L)
1182.5434	1182.5465	-2.6278	501	511	(K)STGCDFAVSPK(L)	1Cys-am
1364.6628	1364.6673	-3.3117	407	418	(K)(M)
1380.6713	1380.6622	6.6128	407	418	(K)YGDLANWMIPGK(M)	1Met-ox
1617.7222	1617.7219	0.2029	111	124	(R)(Q)
1633.7083	1633.7168	-5.2251	111	124	(R)MVSSYVGENAEFER(Q)	1Met-ox
1643.8421	1643.8249	10.4208	391	406	(R)(Y)
1659.8107	1659.8198	-5.5046	391	406	(R)GGHVDLTMLGAMQVSK(Y)	1Met-ox
1675.7797	1675.8148	-20.8909	391	406	(R)GGHVDLTMLGAMQVSK(Y)	2Met-ox
1773.7740	1773.8230	-27.6368	110	124	(K)(Q)
2029.0505	2029.0857	-17.3785	483	500	(K)(S)
2101.1385	2101.1406	-0.9771	84	104	(K)(S)
2211.0961	2211.0248	32.2243	501	520	(K)STGCDFAVSPKLMPMQQIAN(-)	1Met-ox
2233.1251	2233.1352	-4.5456	125	144	(R)(I)
2378.1497	2378.2032	-22.4913	191	211	(R)(A)
2405.1538	2405.1699	-6.6885	369	390	(K)(G)
2421.1669	2421.1648	0.8837	369	390	(K)ETVTILPGASFFSSDESFAMIR(G)	1Met-ox
2595.3150	2595.3095	2.1108	147	173	(R)(Y)
3000.5686	3000.5107	19.2824	147	176	(R)(D)

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The matched peptides cover 46% (241/520AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 47.1 Mean Error = 0.8804 ppm Data Tolerance = 37.1577 ppm Mean Missed Cleavages = 0.1

2. 15/51 matches (29%). 82437.2 Da, pI = 9.14. Acc. # Q9UK10. HUMAN. ZINC FINGER PROTEIN 225.

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
776.4316 776.4055 33.6023 383 389 (R)(H)
1037.4878 1037.5090 -20.3900 35 42 (R)(N)
1053.4765 1053.5039 -26.0117 35 42 (R)EVMLENFR(N) 1Met-ox
1168.5876 1168.6339 -39.6838 383 392 (R)(V)
1182.5434 1182.5425 0.7740 145 154 (K)TETSEGRTCK(K) 1Cys-am
1316.6676 1316.7075 -30.3368 324 335 (K)(M)
1380.6713 1380.6660 3.8316 521 531 (R)(R)
1397.7065 1397.7290 -16.0978 390 401 (R)(C)
1643.8421 1643.7679 45.1255 408 420 (K)(A)
2216.1164 2216.1927 -34.4019 12 30 (K)(K)
2216.1164 2216.1311 -6.6348 156 174 (K)(S)
2216.1164 2216.0730 19.6127 660 678 (K)SIVHSSCLKDQQRDQSGEK(T) 1Cys-am
2283.1815 2283.0837 42.8326 281 298 (R)(S)
2300.1381 2300.0301 46.9628 514 531 (K)(R)
2357.1033 2357.0225 34.2857 542 560 (K)(V)
2405.1538 2405.1627 -3.7044 649 668 (R)EKLLQCEDCGKSIVHSSCLK(D) 1Cys-am
2421.1669 2421.0464 49.7746 402 420 (K)CEECGKGFYTNSQRYSHQR(A) 1Cys-am

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m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
776.4316	776.4055	33.6023	383	389	(R)(H)
1037.4878	1037.5090	-20.3900	35	42	(R)(N)
1053.4765	1053.5039	-26.0117	35	42	(R)EVMLENFR(N)	1Met-ox
1168.5876	1168.6339	-39.6838	383	392	(R)(V)
1182.5434	1182.5425	0.7740	145	154	(K)TETSEGRTCK(K)	1Cys-am
1316.6676	1316.7075	-30.3368	324	335	(K)(M)
1380.6713	1380.6660	3.8316	521	531	(R)(R)
1397.7065	1397.7290	-16.0978	390	401	(R)(C)
1643.8421	1643.7679	45.1255	408	420	(K)(A)
2216.1164	2216.1927	-34.4019	12	30	(K)(K)
2216.1164	2216.1311	-6.6348	156	174	(K)(S)
2216.1164	2216.0730	19.6127	660	678	(K)SIVHSSCLKDQQRDQSGEK(T)	1Cys-am
2283.1815	2283.0837	42.8326	281	298	(R)(S)
2300.1381	2300.0301	46.9628	514	531	(K)(R)
2357.1033	2357.0225	34.2857	542	560	(K)(V)
2405.1538	2405.1627	-3.7044	649	668	(R)EKLLQCEDCGKSIVHSSCLK(D)	1Cys-am
2421.1669	2421.0464	49.7746	402	420	(K)CEECGKGFYTNSQRYSHQR(A)	1Cys-am

The matched peptides cover 27% (191/706AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 29.4 Mean Error = 5.8553 ppm Data Tolerance = 62.7764 ppm Mean Missed Cleavages = 1.1

3. 11/51 matches (21%). 73052.2 Da, pI = 9.33. Acc. # P04049. HUMAN. RAF proto-oncogene serine/threonine-protein kinase (RAF-1) (C-RAF).

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
776.4316 776.4419 -13.2611 101 106 (R)(G)
1099.5632 1099.5359 24.8166 1 9 (-)(T)
1168.5876 1168.5495 32.6025 149 157 (K)LAFCDICQK(F) 1Cys-am
1182.5434 1182.5651 -18.3791 149 157 (K)LAFCDICQK(F) 2Cys-am
1364.6628 1364.6455 12.6494 90 100 (R)GLQPECCAVFR(L) 2Cys-am
1397.7065 1397.7251 -13.3507 440 450 (K)FQMFQLIDIAR(Q) 1Met-ox
1617.7222 1617.7915 -42.8290 74 87 (R)(V)
1633.7083 1633.7864 -47.8355 74 87 (R)NGMSLHDCLMKALK(V) 1Met-ox
1643.8421 1643.8289 7.9736 145 157 (K)(F)
2029.0505 2029.0024 23.6847 257 275 (R)(M)
2300.1381 2300.2041 -28.7145 68 87 (R)TVVNVRNGMSLHDCLMKALK(V) 1Cys-am

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m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
776.4316	776.4419	-13.2611	101	106	(R)(G)
1099.5632	1099.5359	24.8166	1	9	(-)(T)
1168.5876	1168.5495	32.6025	149	157	(K)LAFCDICQK(F)	1Cys-am
1182.5434	1182.5651	-18.3791	149	157	(K)LAFCDICQK(F)	2Cys-am
1364.6628	1364.6455	12.6494	90	100	(R)GLQPECCAVFR(L)	2Cys-am
1397.7065	1397.7251	-13.3507	440	450	(K)FQMFQLIDIAR(Q)	1Met-ox
1617.7222	1617.7915	-42.8290	74	87	(R)(V)
1633.7083	1633.7864	-47.8355	74	87	(R)NGMSLHDCLMKALK(V)	1Met-ox
1643.8421	1643.8289	7.9736	145	157	(K)(F)
2029.0505	2029.0024	23.6847	257	275	(R)(M)
2300.1381	2300.2041	-28.7145	68	87	(R)TVVNVRNGMSLHDCLMKALK(V)	1Cys-am

The matched peptides cover 13% (89/648AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 21.6 Mean Error = -5.6948 ppm Data Tolerance = 55.6617 ppm Mean Missed Cleavages = 0.5

4. 9/51 matches (17%). 71389.5 Da, pI = 8.88. Acc. # Q9UK13. HUMAN. ZINC FINGER PROTEIN 221.

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
1037.4878 1037.4838 3.8529 558 565 (R)GERPYNCK(E) 1Cys-am
1168.5876 1168.5825 4.3496 486 495 (K)(H)
1168.5876 1168.5825 4.3496 570 579 (K)(H)
1182.5434 1182.5981 -46.2957 486 495 (K)SFGWASCLLK(H) 1Cys-am
1182.5434 1182.5981 -46.2957 570 579 (K)SFGWASCLLK(H) 1Cys-am
1397.7065 1397.6782 20.2284 312 322 (K)(S)
1595.8056 1595.7791 16.6204 430 442 (K)(S)
2101.1385 2101.0817 27.0333 34 52 (K)(K)
2217.0673 2217.0003 30.2098 384 401 (K)HQMVHTGEKPYNCKECGK(T) 1Cys-am
2283.1815 2283.0689 49.3097 514 532 (K)(L)
2299.1770 2299.1624 6.3409 65 84 (R)(E)

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m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
1037.4878	1037.4838	3.8529	558	565	(R)GERPYNCK(E)	1Cys-am
1168.5876	1168.5825	4.3496	486	495	(K)(H)
1168.5876	1168.5825	4.3496	570	579	(K)(H)
1182.5434	1182.5981	-46.2957	486	495	(K)SFGWASCLLK(H)	1Cys-am
1182.5434	1182.5981	-46.2957	570	579	(K)SFGWASCLLK(H)	1Cys-am
1397.7065	1397.6782	20.2284	312	322	(K)(S)
1595.8056	1595.7791	16.6204	430	442	(K)(S)
2101.1385	2101.0817	27.0333	34	52	(K)(K)
2217.0673	2217.0003	30.2098	384	401	(K)HQMVHTGEKPYNCKECGK(T)	1Cys-am
2283.1815	2283.0689	49.3097	514	532	(K)(L)
2299.1770	2299.1624	6.3409	65	84	(R)(E)

The matched peptides cover 20% (128/617AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 17.6 Mean Error = 6.3367 ppm Data Tolerance = 58.9850 ppm Mean Missed Cleavages = 0.5

5. 8/51 matches (15%). 79223.9 Da, pI = 8.51. Acc. # Q9QUJ7. MOUSE. LONG-CHAIN-FATTY-ACID--COA LIGASE 4 (LONG-CHAIN ACYL-COA SYNTHETASE 4) (LACS 4) (MACS4).

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
776.4316 776.4266 6.3878 93 99 (K)(E)
1380.6713 1380.6734 -1.5234 125 134 (K)WINYLEVNCR(V) 1Cys-am
1617.7222 1617.7430 -12.8581 100 113 (R)(V)
1633.7083 1633.7379 -18.1582 100 113 (R)EILSEENEMQPNGK(V) 1Met-ox
1643.8421 1643.8467 -2.8295 389 401 (K)VQEMNYVQKTLFK(I) 1Met-ox
2217.0673 2217.1082 -18.4222 361 380 (K)(I)
2233.1251 2233.1031 9.8497 361 380 (K)GDCTVLKPTLMAAVPEIMDR(I) 1Met-ox
2617.2818 2617.3516 -26.6430 357 380 (K)(I)

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m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
776.4316	776.4266	6.3878	93	99	(K)(E)
1380.6713	1380.6734	-1.5234	125	134	(K)WINYLEVNCR(V)	1Cys-am
1617.7222	1617.7430	-12.8581	100	113	(R)(V)
1633.7083	1633.7379	-18.1582	100	113	(R)EILSEENEMQPNGK(V)	1Met-ox
1643.8421	1643.8467	-2.8295	389	401	(K)VQEMNYVQKTLFK(I)	1Met-ox
2217.0673	2217.1082	-18.4222	361	380	(K)(I)
2233.1251	2233.1031	9.8497	361	380	(K)GDCTVLKPTLMAAVPEIMDR(I)	1Met-ox
2617.2818	2617.3516	-26.6430	357	380	(K)(I)

The matched peptides cover 9% (68/711AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 15.7 Mean Error = -8.0246 ppm Data Tolerance = 25.9369 ppm Mean Missed Cleavages = 0.5