MS-Fit Search Results

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Sample ID (comment): HSG096
Database searched: SwissProt.1.27.2002
Molecular weight search (1000 - 100000 Da) selects 98142 entries.
Full pI range: 103988 entries.
Species search ( HUMAN MOUSE ) selects 12515 entries.
Combined molecular weight, pI and species searches select 11105 entries.
Pre searches select 11105 entries.
MS-Fit search selects 1567 entries (results displayed for top 5 matches).

Parameters Used in Search

Considered modifications: | Oxidation of M | Protein N-terminus Acetylated | Acrylamide Modified Cys |

4	75.000 ppm	monoisotopic	Trypsin	2	carbamidomethylation	Hydrogen (H)	Free Acid (O H)
Min. # Peptides to Match	Peptide Mass Tolerance (+/-)	Peptide Masses are	Digest Used	Max. # Missed Cleavages	Cysteines Modified by	Peptide N terminus	Peptide C terminus

Result Summary

Rank	MOWSE Score	# (%) Masses Matched	Protein MW (Da)/pI	Species	Protein Name
1	5.43e+009	21/54 (38%)	56665.1 / 6.29	HUMAN	D-3-phosphoglycerate dehydrogenase (PGDH)
2	1.24e+004	9/54 (16%)	51449.0 / 6.51	MOUSE	D-3-phosphoglycerate dehydrogenase (PGDH) (A10)
3	3.17e+003	9/54 (16%)	98620.6 / 7.78	HUMAN	Metabotropic glutamate receptor 3 precursor
4	1.13e+003	9/54 (16%)	85514.7 / 5.54	HUMAN	Neprilysin (Neutral endopeptidase) (NEP) (Enkephalinase) (Common acute lymphocytic leukemia antigen) (CALLA) (Neutral endopeptidase 24.11) (CD10)
5	944	9/54 (16%)	65375.2 / 11.31	HUMAN	Alu subfamily SB2 sequence contamination warning entry

Detailed Results

1. 21/54 matches (38%). 56665.1 Da, pI = 6.29. Acc. # O43175. HUMAN. D-3-phosphoglycerate dehydrogenase (PGDH).

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
733.4326	733.3997	44.8496	1	7	(-)AFANLR(K)	Acet N
861.5294	861.4947	40.3365	1	8	(-)AFANLRK(V)	Acet N
899.6001	899.5678	35.9058	147	155	(K)(I)
986.6495	986.6039	46.2815	462	469	(R)(T)
1099.6584	1099.6111	42.9591	237	247	(R)(A)
1345.8031	1345.7691	25.2919	352	364	(K)(N)
1462.7642	1462.7034	41.5642	9	20	(K)VLISDSLDPCCR(K)	2Cys-am
1488.7825	1488.7294	35.6318	76	90	(R)(K)
1590.8657	1590.7984	42.3279	8	20	(R)KVLISDSLDPCCR(K)	2Cys-am
1590.8657	1590.7984	42.3279	9	21	(K)VLISDSLDPCCRK(I)	2Cys-am
1639.9063	1639.9205	-8.6766	365	380	(K)NAGNCLSPAVIVGLLK(E)	1Cys-am
1654.8450	1654.7821	38.0152	295	308	(R)CGEEIAVQFVDMVK(G)	1Met-ox 1Cys-am
1782.9697	1783.0078	-21.3499	59	75	(K)(A)
1812.8869	1812.9754	-48.8231	231	247	(R)VVNCARGGIVDEGALLR(A)	1Cys-am
1901.0308	1900.9690	32.4965	39	54	(K)EELIAELQDCEGLIVR(S)	1Cys-am
2048.0989	2048.0235	36.8250	271	289	(R)(E)
2062.0906	2062.0391	24.9702	271	289	(R)ALVDHENVISCPHLGASTK(E)	1Cys-am
2231.1542	2231.0767	34.7759	248	268	(R)ALQSGQCAGAALDVFTEEPPR(D)	1Cys-am
2272.2598	2272.1681	40.3303	470	491	(R)(L)
2304.1811	2304.1579	10.0449	470	491	(R)TQTSDPAMLPTMIGLLAEAGVR(L)	2Met-ox
2471.3505	2471.2816	27.8974	34	54	(K)QNLSKEELIAELQDCEGLIVR(S)	1Cys-am
2502.2972	2502.2047	36.9729	248	270	(R)ALQSGQCAGAALDVFTEEPPRDR(A)	1Cys-am

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The matched peptides cover 40% (215/533AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 38.9 Mean Error = 27.3161 ppm Data Tolerance = 48.2005 ppm Mean Missed Cleavages = 0.3

2. 9/54 matches (16%). 51449.0 Da, pI = 6.51. Acc. # Q61753. MOUSE. D-3-phosphoglycerate dehydrogenase (PGDH) (A10).

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
899.6001 899.5678 35.9058 99 107 (K)(I)
1099.6584 1099.6111 42.9591 189 199 (R)(A)
1654.8450 1654.7821 38.0152 247 260 (R)CGEEIAVQFVDMVK(G) 1Met-ox 1Cys-am
1782.9697 1783.0078 -21.3499 11 27 (K)(A)
1812.8869 1812.9754 -48.8231 183 199 (R)VVNCARGGIVDEGALLR(A) 1Cys-am
2048.0989 2048.0235 36.8250 223 241 (R)(E)
2062.0906 2062.0391 24.9702 223 241 (R)ALVDHENVISCPHLGASTK(E) 1Cys-am
2231.1542 2231.0767 34.7759 200 220 (R)ALQSGQCAGAALDVFTEEPPR(D) 1Cys-am
2502.2972 2502.2047 36.9729 200 222 (R)ALQSGQCAGAALDVFTEEPPRDR(A) 1Cys-am

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m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
899.6001	899.5678	35.9058	99	107	(K)(I)
1099.6584	1099.6111	42.9591	189	199	(R)(A)
1654.8450	1654.7821	38.0152	247	260	(R)CGEEIAVQFVDMVK(G)	1Met-ox 1Cys-am
1782.9697	1783.0078	-21.3499	11	27	(K)(A)
1812.8869	1812.9754	-48.8231	183	199	(R)VVNCARGGIVDEGALLR(A)	1Cys-am
2048.0989	2048.0235	36.8250	223	241	(R)(E)
2062.0906	2062.0391	24.9702	223	241	(R)ALVDHENVISCPHLGASTK(E)	1Cys-am
2231.1542	2231.0767	34.7759	200	220	(R)ALQSGQCAGAALDVFTEEPPR(D)	1Cys-am
2502.2972	2502.2047	36.9729	200	222	(R)ALQSGQCAGAALDVFTEEPPRDR(A)	1Cys-am

The matched peptides cover 20% (99/485AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 16.7 Mean Error = 20.0279 ppm Data Tolerance = 64.6733 ppm Mean Missed Cleavages = 0.3

3. 9/54 matches (16%). 98620.6 Da, pI = 7.78. Acc. # Q14832. HUMAN. Metabotropic glutamate receptor 3 precursor.

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
702.4297 702.4402 -14.8855 722 727 (R)(C)
1345.8031 1345.7262 57.1794 236 247 (R)NICIATAEKVGR(S) 1Cys-am
1488.7825 1488.7963 -9.3039 16 28 (K)(R)
2048.0989 2047.9579 68.8388 49 66 (K)(L)
2062.0906 2061.9736 56.7659 49 66 (K)GTGTEECGRINEDRGIQR(L) 1Cys-am
2285.2490 2285.1964 23.0337 80 99 (K)DDYLLPGVKLGVHILDTCSR(D) 1Cys-am
2299.2023 2299.2491 -20.3275 428 446 (K)(D)
2473.3629 2473.3349 11.2878 260 280 (R)(E)
2715.4474 2715.3924 20.2791 796 820 (R)(V)

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m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
702.4297	702.4402	-14.8855	722	727	(R)(C)
1345.8031	1345.7262	57.1794	236	247	(R)NICIATAEKVGR(S)	1Cys-am
1488.7825	1488.7963	-9.3039	16	28	(K)(R)
2048.0989	2047.9579	68.8388	49	66	(K)(L)
2062.0906	2061.9736	56.7659	49	66	(K)GTGTEECGRINEDRGIQR(L)	1Cys-am
2285.2490	2285.1964	23.0337	80	99	(K)DDYLLPGVKLGVHILDTCSR(D)	1Cys-am
2299.2023	2299.2491	-20.3275	428	446	(K)(D)
2473.3629	2473.3349	11.2878	260	280	(R)(E)
2715.4474	2715.3924	20.2791	796	820	(R)(V)

The matched peptides cover 15% (134/877AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 16.7 Mean Error = 21.4297 ppm Data Tolerance = 66.6636 ppm Mean Missed Cleavages = 1.1

4. 9/54 matches (16%). 85514.7 Da, pI = 5.54. Acc. # P08473. HUMAN. Neprilysin (Neutral endopeptidase) (NEP) (Enkephalinase) (Common acute lymphocytic leukemia antigen) (CALLA) (Neutral endopeptidase 24.11) (CD10).

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
1320.7300 1320.7640 -25.7222 187 198 (K)(V)
1639.9063 1639.8015 63.9246 362 374 (K)(F)
1654.8450 1654.7964 29.3203 504 516 (K)(F)
2032.0692 2032.0537 7.5904 375 391 (R)(N)
2048.0989 2048.0487 24.5436 375 391 (R)FIMDLVSSLSRTYKESR(N) 1Met-ox
2231.1542 2231.1672 -5.8005 473 492 (K)(L)
2283.2550 2283.1219 58.2991 449 467 (R)(R)
2299.2023 2299.1168 37.2044 449 467 (R)EVFIQTLDDLTWMDAETKK(R) 1Met-ox
2601.4558 2601.3201 52.1825 504 524 (K)(K)

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m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
1320.7300	1320.7640	-25.7222	187	198	(K)(V)
1639.9063	1639.8015	63.9246	362	374	(K)(F)
1654.8450	1654.7964	29.3203	504	516	(K)(F)
2032.0692	2032.0537	7.5904	375	391	(R)(N)
2048.0989	2048.0487	24.5436	375	391	(R)FIMDLVSSLSRTYKESR(N)	1Met-ox
2231.1542	2231.1672	-5.8005	473	492	(K)(L)
2283.2550	2283.1219	58.2991	449	467	(R)(R)
2299.2023	2299.1168	37.2044	449	467	(R)EVFIQTLDDLTWMDAETKK(R)	1Met-ox
2601.4558	2601.3201	52.1825	504	524	(K)(K)

The matched peptides cover 13% (102/750AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 16.7 Mean Error = 26.8380 ppm Data Tolerance = 60.5176 ppm Mean Missed Cleavages = 1.4

5. 9/54 matches (16%). 65375.2 Da, pI = 11.31. Acc. # P39191. HUMAN. Alu subfamily SB2 sequence contamination warning entry.

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
899.6001 899.5678 35.9058 53 60 (R)(L)
1462.7642 1462.7688 -3.1319 166 178 (R)MAXTREAELAVSR(D) 1Met-ox
1575.8904 1575.8694 13.3039 454 465 (R)(D)
1639.9063 1639.8080 59.9451 253 268 (R)(E)
2032.0692 2032.1126 -21.3710 199 218 (K)(G)
2062.0906 2062.1885 -47.4701 273 291 (K)(Q)
2211.1715 2211.1053 29.9579 61 80 (R)LRQENGVNPGSGACSEPRLR(H) 1Cys-am
2304.1811 2304.1710 4.3856 433 455 (R)(A)
2473.3629 2473.4003 -15.1448 269 291 (R)(Q)

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Click individual mass to do a non-specific cleavage search.

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
899.6001	899.5678	35.9058	53	60	(R)(L)
1462.7642	1462.7688	-3.1319	166	178	(R)MAXTREAELAVSR(D)	1Met-ox
1575.8904	1575.8694	13.3039	454	465	(R)(D)
1639.9063	1639.8080	59.9451	253	268	(R)(E)
2032.0692	2032.1126	-21.3710	199	218	(K)(G)
2062.0906	2062.1885	-47.4701	273	291	(K)(Q)
2211.1715	2211.1053	29.9579	61	80	(R)LRQENGVNPGSGACSEPRLR(H)	1Cys-am
2304.1811	2304.1710	4.3856	433	455	(R)(A)
2473.3629	2473.4003	-15.1448	269	291	(R)(Q)

The matched peptides cover 22% (133/603AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 16.7 Mean Error = 6.2645 ppm Data Tolerance = 65.4693 ppm Mean Missed Cleavages = 1.2