MS-Fit Search Results


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Sample ID (comment): HSG097
Database searched: SwissProt.1.27.2002
Molecular weight search (1000 - 100000 Da) selects 98142 entries.
Full pI range: 103988 entries.
Species search ( HUMAN MOUSE ) selects 12515 entries.
Combined molecular weight, pI and species searches select 11105 entries.
Pre searches select 11105 entries.
MS-Fit search selects 1624 entries (results displayed for top 5 matches).

Parameters Used in Search

Considered modifications: | Oxidation of M | Protein N-terminus Acetylated | Acrylamide Modified Cys |
Min. # Peptides
to Match
Peptide Mass
Tolerance (+/-)
Peptide Masses
are
Digest
Used
Max. # Missed
Cleavages
Cysteines
Modified by
Peptide
N terminus
Peptide
C terminus
4 50.000 ppm monoisotopic Trypsin 2 carbamidomethylation Hydrogen (H) Free Acid (O H)

Result Summary

RankMOWSE
Score
# (%)
Masses
Matched
Protein
MW (Da)/pI
SpeciesAccession #Protein Name
14.68e+01126/65 (40%) 56665.1 / 6.29HUMAND-3-phosphoglycerate dehydrogenase (PGDH)
24.59e+00718/65 (27%) 59266.3 / 6.42HUMANGlucose-6-phosphate 1-dehydrogenase (G6PD)
32.61e+00410/65 (15%) 59263.1 / 6.06MOUSEGLUCOSE-6-PHOSPHATE 1-DEHYDROGENASE X (G6PD)
41.93e+0049/65 (13%) 51449.0 / 6.51MOUSED-3-phosphoglycerate dehydrogenase (PGDH) (A10)
51.86e+0048/65 (12%) 41598.0 / 4.69HUMANX/potassium-transporting ATPase beta-m chain (X,K-ATPase beta-m subunit)

Detailed Results

1. 26/65 matches (40%). 56665.1 Da, pI = 6.29. Acc. # O43175. HUMAN. D-3-phosphoglycerate dehydrogenase (PGDH).
m/z
submitted
MH+
matched
Delta
ppm
startendPeptide Sequence
(Click for Fragment Ions)
Modifications
671.4346671.420421.0601 70 75(K)(A)
733.4207733.399728.5992 1 7(-)AFANLR(K)Acet N
861.5191861.494728.3631 1 8(-)AFANLRK(V)Acet N
899.5865899.567820.7129 147 155(K)(I)
986.6315986.603928.0219 462 469(R)(T)
1099.64221099.611128.2021 237 247(R)(A)
1130.63911130.605729.5037 59 69(K)(L)
1345.79841345.769121.7943 352 364(K)(N)
1390.68061390.7000-13.9448 120 132(R)QIPQATASMKDGK(W)1Met-ox
1390.68061390.654418.8663 523 533(K)(-)
1462.73861462.703424.0371 9 20(K)VLISDSLDPCCR(K)2Cys-am
1488.76251488.729422.2338 76 90(R)(K)
1590.82911590.798419.3206 8 20(R)KVLISDSLDPCCR(K)2Cys-am
1590.82911590.798419.3206 9 21(K)VLISDSLDPCCRK(I)2Cys-am
1638.81701638.787118.2498 295 308(R)CGEEIAVQFVDMVK(G)1Cys-am
1639.90471639.9205-9.6522 365 380(K)NAGNCLSPAVIVGLLK(E)1Cys-am
1654.81571654.782120.3549 295 308(R)CGEEIAVQFVDMVK(G)1Met-ox 1Cys-am
1718.90281718.89335.5240 8 21(R)KVLISDSLDPCCRK(I)2Cys-am
1886.96841886.95347.9794 39 54(K)(S)
1901.01101900.969022.1139 39 54(K)EELIAELQDCEGLIVR(S)1Cys-am
2062.07682062.039118.2637 271 289(R)ALVDHENVISCPHLGASTK(E)1Cys-am
2231.10592231.076713.1285 248 268(R)ALQSGQCAGAALDVFTEEPPR(D)1Cys-am
2272.20472272.168116.1019 470 491(R)(L)
2288.21392288.180814.4530 39 58(K)EELIAELQDCEGLIVRSATK(V)1Cys-am
2288.21392288.163022.2142 470 491(R)TQTSDPAMLPTMIGLLAEAGVR(L)1Met-ox
2304.18692304.157912.5590 470 491(R)TQTSDPAMLPTMIGLLAEAGVR(L)2Met-ox
2457.25122457.2659-5.9910 34 54(K)(S)
2471.31402471.281613.1136 34 54(K)QNLSKEELIAELQDCEGLIVR(S)1Cys-am
2502.26372502.204723.5851 248 270(R)ALQSGQCAGAALDVFTEEPPRDR(A)1Cys-am

Click link below to search for another component.

Click individual mass to do a non-specific cleavage search.

The matched peptides cover 44% (237/533AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 40.0 Mean Error = 16.8306 ppm Data Tolerance = 22.0012 ppm Mean Missed Cleavages = 0.3

2. 18/65 matches (27%). 59266.3 Da, pI = 6.42. Acc. # P11413. HUMAN. Glucose-6-phosphate 1-dehydrogenase (G6PD).
m/z
submitted
MH+
matched
Delta
ppm
startendPeptide Sequence
(Click for Fragment Ions)
Modifications
899.5865899.542748.6745 387 393(K)(V)
1002.58541002.552532.8216 220 227(R)(D)
1058.65961058.636222.0858 167 175(R)(D)
1102.62501102.604918.1542 349 357(R)(C)
1138.64731138.626118.6624 96 104(K)(N)
1173.66791173.638025.4737 183 192(R)(E)
1273.65511273.621726.2296 247 257(R)(D)
1394.77951394.762512.1688 48 57(K)(D)
1447.69401447.7520-40.0939 349 360(R)(A)
1664.88941664.864814.7771 58 72(R)(S)
1745.83301745.785227.3955 153 166(K)NIHESCMSQIGWNR(I)1Cys-am
1761.80681761.780115.1574 153 166(K)NIHESCMSQIGWNR(I)1Met-ox 1Cys-am
1807.80851807.788710.9096 105 120(R)(L)
1835.93141835.93010.7415 440 454(R)LILDVFCGSQMHFVR(S)1Cys-am
1928.03901927.976532.4213 331 348(R)(W)
1956.96151957.0112-25.3763 1 17(-)MAEQVALSRTHVCGILR(E)1Met-ox
1956.96151956.939011.4758 371 386(R)LQFHDVAGDIFHQQCK(R)1Cys-am
2181.18162181.101436.7661 228 246(R)DNIACVILTFKEPFGTEGR(G)1Cys-am
2344.09012344.0920-0.7828 408 427(K)(Y)

Click link below to search for another component.

Click individual mass to do a non-specific cleavage search.

The matched peptides cover 43% (226/515AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 27.7 Mean Error = 15.1401 ppm Data Tolerance = 41.7578 ppm Mean Missed Cleavages = 0.3

3. 10/65 matches (15%). 59263.1 Da, pI = 6.06. Acc. # Q00612. MOUSE. GLUCOSE-6-PHOSPHATE 1-DEHYDROGENASE X (G6PD).
m/z
submitted
MH+
matched
Delta
ppm
startendPeptide Sequence
(Click for Fragment Ions)
Modifications
899.5865899.542748.6745 387 393(K)(V)
1002.58541002.552532.8216 220 227(R)(D)
1058.65961058.636222.0858 167 175(R)(D)
1102.62501102.604918.1542 349 357(R)(C)
1273.65511273.621726.2296 247 257(R)(D)
1394.77951394.762512.1688 48 57(K)(D)
1447.69401447.7520-40.0939 349 360(R)(A)
1835.93141835.93010.7415 440 454(R)LILDVFCGSQMHFVR(S)1Cys-am
2181.18162181.101436.7661 228 246(R)DNIACVILTFKEPFGTEGR(G)1Cys-am
2344.09012344.0920-0.7828 408 427(K)(Y)

Click link below to search for another component.

Click individual mass to do a non-specific cleavage search.

The matched peptides cover 21% (111/515AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 15.4 Mean Error = 15.6765 ppm Data Tolerance = 49.9012 ppm Mean Missed Cleavages = 0.3

4. 9/65 matches (13%). 51449.0 Da, pI = 6.51. Acc. # Q61753. MOUSE. D-3-phosphoglycerate dehydrogenase (PGDH) (A10).
m/z
submitted
MH+
matched
Delta
ppm
startendPeptide Sequence
(Click for Fragment Ions)
Modifications
671.4346671.420421.0601 22 27(K)(A)
899.5865899.567820.7129 99 107(K)(I)
1099.64221099.611128.2021 189 199(R)(A)
1130.63911130.605729.5037 11 21(K)(L)
1638.81701638.787118.2498 247 260(R)CGEEIAVQFVDMVK(G)1Cys-am
1654.81571654.782120.3549 247 260(R)CGEEIAVQFVDMVK(G)1Met-ox 1Cys-am
2062.07682062.039118.2637 223 241(R)ALVDHENVISCPHLGASTK(E)1Cys-am
2231.10592231.076713.1285 200 220(R)ALQSGQCAGAALDVFTEEPPR(D)1Cys-am
2502.26372502.204723.5851 200 222(R)ALQSGQCAGAALDVFTEEPPRDR(A)1Cys-am

Click link below to search for another component.

Click individual mass to do a non-specific cleavage search.

The matched peptides cover 19% (93/485AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 13.8 Mean Error = 21.4512 ppm Data Tolerance = 10.1623 ppm Mean Missed Cleavages = 0.1

5. 8/65 matches (12%). 41598.0 Da, pI = 4.69. Acc. # Q9UN42. HUMAN. X/potassium-transporting ATPase beta-m chain (X,K-ATPase beta-m subunit).
m/z
submitted
MH+
matched
Delta
ppm
startendPeptide Sequence
(Click for Fragment Ions)
Modifications
1298.75821298.704341.4801 219 228(K)ACQFKRSFLK(N)1Cys-am
1426.87091426.805845.5908 254 266(R)(V)
1835.93141835.911410.8873 86 99(K)(M)
1901.01101901.0319-10.9610 254 270(R)(V)
2272.20472272.180010.8710 302 321(K)(N)
2288.21392288.174917.0198 302 321(K)LTHVNYTSPLVAMHFTDVVK(N)1Met-ox
2454.28542454.242617.4461 86 104(K)(T)
2457.25122457.143044.0230 229 250(K)(M)

Click link below to search for another component.

Click individual mass to do a non-specific cleavage search.

The matched peptides cover 24% (88/357AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 12.3 Mean Error = 22.0446 ppm Data Tolerance = 40.0022 ppm Mean Missed Cleavages = 0.8


MS-Fit 3.3.1, ProteinProspector 3.4.1
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