MS-Fit Search Results

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Sample ID (comment): HSG098
Database searched: SwissProt.1.27.2002
Molecular weight search (1000 - 100000 Da) selects 98142 entries.
Full pI range: 103988 entries.
Species search ( HUMAN MOUSE ) selects 12515 entries.
Combined molecular weight, pI and species searches select 11105 entries.
Pre searches select 11105 entries.
MS-Fit search selects 1062 entries (results displayed for top 5 matches).

Parameters Used in Search

Considered modifications: | Oxidation of M | Protein N-terminus Acetylated | Acrylamide Modified Cys |

4	50.000 ppm	monoisotopic	Trypsin	2	carbamidomethylation	Hydrogen (H)	Free Acid (O H)
Min. # Peptides to Match	Peptide Mass Tolerance (+/-)	Peptide Masses are	Digest Used	Max. # Missed Cleavages	Cysteines Modified by	Peptide N terminus	Peptide C terminus

Result Summary

Rank	MOWSE Score	# (%) Masses Matched	Protein MW (Da)/pI	Species	Protein Name
1	4.67e+006	24/54 (44%)	62639.8 / 6.40	HUMAN	Stress-induced-phosphoprotein 1 (STI1) (Hsp70/Hsp90-organizing protein) (Transformation-sensitive protein IEF SSP 3521)
2	1.63e+004	9/54 (16%)	64466.2 / 6.39	HUMAN	Bifunctional purine biosynthesis protein PURH [Includes: Phosphoribosylaminoimidazolecarboxamide formyltransferase (AICAR transformylase); IMP cyclohydrolase (Inosinicase) (IMP synthetase) (ATIC)]
3	4.34e+003	7/54 (12%)	58628.9 / 9.06	MOUSE	T-box transcription factor TBX6 (T-box protein 6)
4	1.14e+003	7/54 (12%)	67036.3 / 9.00	MOUSE	Zinc finger protein 35 (Zfp-35)
5	1.12e+003	7/54 (12%)	51200.1 / 5.76	HUMAN	BAG-FAMILY MOLECULAR CHAPERONE REGULATOR-5 (BAG-5)

Detailed Results

1. 24/54 matches (44%). 62639.8 Da, pI = 6.40. Acc. # P31948. HUMAN. Stress-induced-phosphoprotein 1 (STI1) (Hsp70/Hsp90-organizing protein) (Transformation-sensitive protein IEF SSP 3521).

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
900.5140	900.5155	-1.5962	154	160	(R)(N)
1008.5015	1008.4607	40.5247	278	284	(K)CRELCEK(A)	1Cys-am
1008.5015	1008.4672	34.0541	454	462	(K)(E)
1047.5099	1047.4893	19.6413	110	118	(K)(L)
1065.5383	1065.5580	-18.5006	145	153	(R)(E)
1087.4080	1087.4487	-37.4441	471	478	(R)CMMAQYNR(H)	1Cys-am
1100.6507	1100.6502	0.4345	534	543	(K)(-)
1132.6512	1132.6479	2.9245	78	87	(R)(F)
1214.6198	1214.6057	11.5577	306	315	(R)(Y)
1298.6387	1298.6533	-11.2415	316	325	(K)(S)
1464.7243	1464.7348	-7.1856	33	44	(K)(S)
1488.7864	1488.7950	-5.7411	352	364	(R)(N)
1608.7941	1608.8386	-27.6550	79	92	(K)(R)
1736.9075	1736.9335	-14.9933	78	92	(R)(R)
1764.9037	1764.9397	-20.4072	79	93	(K)(T)
1803.8037	1803.8562	-29.0988	123	136	(R)KFMNPFNMPNLYQK(L)	2Met-ox
1809.7888	1809.8403	-28.4629	416	429	(K)DCEECIQLEPTFIK(G)	2Cys-am
1870.8888	1870.9299	-21.9632	94	109	(R)(E)
1887.9721	1888.0292	-30.2536	514	530	(K)(I)
1905.8484	1905.8509	-1.3205	489	505	(R)AMADPEVQQIMSDPAMR(L)	1Met-ox
1912.8630	1912.8711	-4.2466	454	470	(K)ALDLDSSCKEAADGYQR(C)	1Cys-am
1921.8403	1921.8458	-2.8823	489	505	(R)AMADPEVQQIMSDPAMR(L)	2Met-ox
1937.8422	1937.8407	0.7457	489	505	(R)AMADPEVQQIMSDPAMR(L)	3Met-ox
2080.9899	2081.0225	-15.6608	14	32	(K)ALSVGNIDDALQCYSEAIK(L)	1Cys-am
2364.0976	2364.1070	-3.9724	253	272	(K)ELDPTNMTYITNQAAVYFEK(G)	1Met-ox

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The matched peptides cover 45% (245/543AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 44.4 Mean Error = -6.9097 ppm Data Tolerance = 38.3939 ppm Mean Missed Cleavages = 0.5

2. 9/54 matches (16%). 64466.2 Da, pI = 6.39. Acc. # P31939. HUMAN. Bifunctional purine biosynthesis protein PURH [Includes: Phosphoribosylaminoimidazolecarboxamide formyltransferase (AICAR transformylase); IMP cyclohydrolase (Inosinicase) (IMP synthetase) (ATIC)].

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
890.4705 890.5100 -44.3466 90 96 (R)(V)
900.5140 900.4791 38.8106 408 415 (K)(D)
1008.5015 1008.5056 -3.9984 461 467 (K)(H)
1464.7243 1464.7334 -6.2726 553 568 (R)(V)
1623.7833 1623.7688 8.8942 49 63 (R)DVSELTGFPEMLGGR(V) 1Met-ox
1715.8395 1715.8645 -14.5203 530 544 (K)(D)
1736.9075 1736.9634 -32.1842 468 482 (R)(R)
2034.9033 2034.9197 -8.0941 177 193 (K)(K)
2553.3556 2553.3776 -8.6029 108 132 (K)(A)

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m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
890.4705	890.5100	-44.3466	90	96	(R)(V)
900.5140	900.4791	38.8106	408	415	(K)(D)
1008.5015	1008.5056	-3.9984	461	467	(K)(H)
1464.7243	1464.7334	-6.2726	553	568	(R)(V)
1623.7833	1623.7688	8.8942	49	63	(R)DVSELTGFPEMLGGR(V)	1Met-ox
1715.8395	1715.8645	-14.5203	530	544	(K)(D)
1736.9075	1736.9634	-32.1842	468	482	(R)(R)
2034.9033	2034.9197	-8.0941	177	193	(K)(K)
2553.3556	2553.3776	-8.6029	108	132	(K)(A)

The matched peptides cover 21% (125/591AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 16.7 Mean Error = -7.8127 ppm Data Tolerance = 47.1390 ppm Mean Missed Cleavages = 0.2

3. 7/54 matches (12%). 58628.9 Da, pI = 9.06. Acc. # P70327. MOUSE. T-box transcription factor TBX6 (T-box protein 6).

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
1047.5099 1047.5118 -1.8069 273 280 (R)ENGRNCKR(E) 1Cys-am
1870.8888 1870.9339 -24.1133 241 256 (R)(I)
1905.8484 1905.9315 -43.6271 120 136 (R)(Y)
1921.8403 1921.9264 -44.8367 120 136 (R)MFPACRVSVTGLDPEAR(Y) 1Met-ox
2211.0927 2211.0835 4.1992 17 37 (R)(Y)
2283.1354 2283.2171 -35.8051 373 393 (R)LQTLAPSPLLSCISGPTAWTR(G) 1Cys-am
2284.1407 2284.1616 -9.1415 394 413 (R)GLCLSGSSICTILCPTLHPR(G) 3Cys-am

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m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
1047.5099	1047.5118	-1.8069	273	280	(R)ENGRNCKR(E)	1Cys-am
1870.8888	1870.9339	-24.1133	241	256	(R)(I)
1905.8484	1905.9315	-43.6271	120	136	(R)(Y)
1921.8403	1921.9264	-44.8367	120	136	(R)MFPACRVSVTGLDPEAR(Y)	1Met-ox
2211.0927	2211.0835	4.1992	17	37	(R)(Y)
2283.1354	2283.2171	-35.8051	373	393	(R)LQTLAPSPLLSCISGPTAWTR(G)	1Cys-am
2284.1407	2284.1616	-9.1415	394	413	(R)GLCLSGSSICTILCPTLHPR(G)	3Cys-am

The matched peptides cover 19% (103/540AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 13.0 Mean Error = -22.1616 ppm Data Tolerance = 40.3618 ppm Mean Missed Cleavages = 0.6

4. 7/54 matches (12%). 67036.3 Da, pI = 9.00. Acc. # P15620. MOUSE. Zinc finger protein 35 (Zfp-35).

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
1736.9075 1736.8286 45.4132 517 530 (R)IHTGEKPYKCMCGK(A) 2Cys-am
1803.8037 1803.7907 7.2460 461 475 (R)VHSGEKPYHCSSCNK(A) 1Cys-am
1937.8422 1937.8461 -2.0203 209 223 (R)(H)
2211.0927 2210.9936 44.8346 458 475 (K)(A)
2233.0644 2232.9775 38.9319 526 543 (K)CMCGKAFSQCSAFTLHQR(I) 1Met-ox 2Cys-am
2283.1354 2283.0650 30.7991 405 423 (R)IHTGEKPYPCNSCSKSFSR(G) 2Cys-am
2364.0976 2364.0025 40.2102 77 95 (R)QYNCDECDQSFAWSTGLIR(H) 1Cys-am

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m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
1736.9075	1736.8286	45.4132	517	530	(R)IHTGEKPYKCMCGK(A)	2Cys-am
1803.8037	1803.7907	7.2460	461	475	(R)VHSGEKPYHCSSCNK(A)	1Cys-am
1937.8422	1937.8461	-2.0203	209	223	(R)(H)
2211.0927	2210.9936	44.8346	458	475	(K)(A)
2233.0644	2232.9775	38.9319	526	543	(K)CMCGKAFSQCSAFTLHQR(I)	1Met-ox 2Cys-am
2283.1354	2283.0650	30.7991	405	423	(R)IHTGEKPYPCNSCSKSFSR(G)	2Cys-am
2364.0976	2364.0025	40.2102	77	95	(R)QYNCDECDQSFAWSTGLIR(H)	1Cys-am