MS-Fit Search Results

Press stop on your browser if you wish to abort this MS-Fit search prematurely.
Sample ID (comment): HSG099
Database searched: SwissProt.1.27.2002
Molecular weight search (1000 - 100000 Da) selects 98142 entries.
Full pI range: 103988 entries.
Species search ( HUMAN MOUSE ) selects 12515 entries.
Combined molecular weight, pI and species searches select 11105 entries.
Pre searches select 11105 entries.
MS-Fit search selects 1125 entries (results displayed for top 5 matches).

Parameters Used in Search

Considered modifications: | Oxidation of M | Protein N-terminus Acetylated | Acrylamide Modified Cys |

4	75.000 ppm	monoisotopic	Trypsin	2	carbamidomethylation	Hydrogen (H)	Free Acid (O H)
Min. # Peptides to Match	Peptide Mass Tolerance (+/-)	Peptide Masses are	Digest Used	Max. # Missed Cleavages	Cysteines Modified by	Peptide N terminus	Peptide C terminus

Result Summary

Rank	MOWSE Score	# (%) Masses Matched	Protein MW (Da)/pI	Species	Protein Name
1	1.62e+006	15/53 (28%)	66018.2 / 8.16	HUMAN	Keratin, type II cytoskeletal 1 (Cytokeratin 1) (K1) (CK 1) (67 kDa cytokeratin) (Hair alpha protein)
2	8.24e+005	14/53 (26%)	61987.8 / 5.14	HUMAN	KERATIN, TYPE I CYTOSKELETAL 9 (CYTOKERATIN 9) (K9) (CK 9)
3	1.25e+004	9/53 (16%)	53734.0 / 9.15	HUMAN	HYPOTHETICAL PROTEIN DJ1198H6.1
4	1.2e+004	11/53 (20%)	67255.0 / 9.06	MOUSE	ZINC FINGER PROTEIN 37 (ZFP-37) (MALE GERM CELL SPECIFIC ZINC FINGER PROTEIN)
5	9.85e+003	10/53 (18%)	89614.9 / 8.61	HUMAN	Tumor necrosis factor, alpha-induced protein 3 (Putative DNA binding protein A20) (Zinc finger protein A20)

Detailed Results

1. 15/53 matches (28%). 66018.2 Da, pI = 8.16. Acc. # P04264. HUMAN. Keratin, type II cytoskeletal 1 (Cytokeratin 1) (K1) (CK 1) (67 kDa cytokeratin) (Hair alpha protein).

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
1157.5590	1157.5149	38.1403	464	472	(R)DYQELMNTK(L)	1Met-ox
1179.5518	1179.6010	-41.7140	377	386	(K)(H)
1265.5945	1265.6378	-34.1744	278	288	(R)(K)
1277.6367	1277.6337	2.3014	247	257	(K)(N)
1277.6367	1277.7105	-57.8013	473	483	(K)(T)
1300.4820	1300.5302	-37.0384	258	267	(K)(N)
1372.6783	1372.6722	4.4885	1	12	(-)SRQFSSRSGYR(S)	Acet N
1383.6152	1383.6909	-54.6412	186	197	(K)(V)
1393.6677	1393.7327	-46.6405	278	289	(R)(D)
1475.6977	1475.7858	-59.7001	200	211	(R)(W)
1475.6977	1475.7494	-35.0460	212	223	(K)(T)
1638.7841	1638.8604	-46.5223	186	199	(K)(F)
1716.7775	1716.8517	-43.1897	418	432	(K)(G)
1765.6514	1765.7353	-47.5169	46	65	(R)(S)
1993.9060	1993.9772	-35.7141	224	239	(R)(R)
2150.0158	2150.0783	-29.0586	224	240	(R)(R)
2383.8554	2383.9524	-40.6837	519	549	(R)(G)

Click link below to search for another component.

Click individual mass to do a non-specific cleavage search.

The matched peptides cover 30% (196/644AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 28.3 Mean Error = -33.2065 ppm Data Tolerance = 50.9200 ppm Mean Missed Cleavages = 0.4

2. 14/53 matches (26%). 61987.8 Da, pI = 5.14. Acc. # P35527. HUMAN. KERATIN, TYPE I CYTOSKELETAL 9 (CYTOKERATIN 9) (K9) (CK 9).

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
897.3618 897.4140 -58.2271 233 239 (R)(I)
1157.5590 1157.5915 -28.0435 250 260 (R)(Q)
1277.6367 1277.5875 38.5272 29 40 (R)(G)
1307.6245 1307.6782 -41.0670 240 249 (R)(Q)
1707.6762 1707.7509 -43.7619 46 62 (R)(G)
1791.6320 1791.7283 -53.7044 490 512 (R)(G)
1837.8562 1837.9660 -59.6985 374 389 (R)(K)
1851.8468 1851.9275 -43.5756 321 335 (K)(N)
1965.9991 1966.0609 -31.4409 374 390 (R)(A)
2299.0513 2299.1202 -29.9539 271 289 (K)(N)
2510.0429 2510.1323 -35.6298 449 471 (K)(I)
2705.1050 2705.1617 -20.9438 63 94 (R)(G)
2902.2721 2902.4111 -47.8593 199 223 (K)(T)
3264.3401 3264.5144 -53.4052 339 367 (K)(E)

Click link below to search for another component.

Click individual mass to do a non-specific cleavage search.

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)
897.3618	897.4140	-58.2271	233	239	(R)(I)
1157.5590	1157.5915	-28.0435	250	260	(R)(Q)
1277.6367	1277.5875	38.5272	29	40	(R)(G)
1307.6245	1307.6782	-41.0670	240	249	(R)(Q)
1707.6762	1707.7509	-43.7619	46	62	(R)(G)
1791.6320	1791.7283	-53.7044	490	512	(R)(G)
1837.8562	1837.9660	-59.6985	374	389	(R)(K)
1851.8468	1851.9275	-43.5756	321	335	(K)(N)
1965.9991	1966.0609	-31.4409	374	390	(R)(A)
2299.0513	2299.1202	-29.9539	271	289	(K)(N)
2510.0429	2510.1323	-35.6298	449	471	(K)(I)
2705.1050	2705.1617	-20.9438	63	94	(R)(G)
2902.2721	2902.4111	-47.8593	199	223	(K)(T)
3264.3401	3264.5144	-53.4052	339	367	(K)(E)

The matched peptides cover 38% (240/622AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 26.4 Mean Error = -36.3417 ppm Data Tolerance = 49.1658 ppm Mean Missed Cleavages = 0.5

3. 9/53 matches (16%). 53734.0 Da, pI = 9.15. Acc. # O95521. HUMAN. HYPOTHETICAL PROTEIN DJ1198H6.1.

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
1302.5854 1302.6635 -59.9338 166 174 (R)(R)
1393.6677 1393.6574 7.3684 376 386 (R)CSQLTTFYFGR(N) 1Cys-am
1393.6677 1393.6932 -18.2713 435 445 (R)AELMCTLREVR(Q) 1Met-ox
1475.6977 1475.7139 -10.9791 449 461 (K)RIFIGPTPCPSCG(-) 1Cys-am
1837.8562 1837.8583 -1.1359 116 131 (R)(R)
1851.8468 1851.8740 -14.6914 116 131 (R)WPGAWALSCFPETTSK(R) 1Cys-am
1993.9060 1993.9594 -26.8083 116 132 (R)(Q)
2175.9888 2176.1146 -57.8148 149 165 (K)VFIDICLKEIPQDECLR(Y) 2Cys-am
2233.0476 2233.0573 -4.3067 237 254 (K)LVFSRCHHYTSDNELQGR(L) 1Cys-am
2527.1521 2527.2855 -52.7724 166 184 (R)YLFQWVYQRRGLVHLCCSK(L) 1Cys-am

Click link below to search for another component.

Click individual mass to do a non-specific cleavage search.

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
1302.5854	1302.6635	-59.9338	166	174	(R)(R)
1393.6677	1393.6574	7.3684	376	386	(R)CSQLTTFYFGR(N)	1Cys-am
1393.6677	1393.6932	-18.2713	435	445	(R)AELMCTLREVR(Q)	1Met-ox
1475.6977	1475.7139	-10.9791	449	461	(K)RIFIGPTPCPSCG(-)	1Cys-am
1837.8562	1837.8583	-1.1359	116	131	(R)(R)
1851.8468	1851.8740	-14.6914	116	131	(R)WPGAWALSCFPETTSK(R)	1Cys-am
1993.9060	1993.9594	-26.8083	116	132	(R)(Q)
2175.9888	2176.1146	-57.8148	149	165	(K)VFIDICLKEIPQDECLR(Y)	2Cys-am
2233.0476	2233.0573	-4.3067	237	254	(K)LVFSRCHHYTSDNELQGR(L)	1Cys-am
2527.1521	2527.2855	-52.7724	166	184	(R)YLFQWVYQRRGLVHLCCSK(L)	1Cys-am

The matched peptides cover 22% (106/461AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 17.0 Mean Error = -23.9345 ppm Data Tolerance = 49.2340 ppm Mean Missed Cleavages = 0.7

4. 11/53 matches (20%). 67255.0 Da, pI = 9.06. Acc. # P17141. MOUSE. ZINC FINGER PROTEIN 37 (ZFP-37) (MALE GERM CELL SPECIFIC ZINC FINGER PROTEIN).

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
897.3618 897.4253 -70.7437 371 377 (K)ECGKSFR(Y) 1Cys-am
1157.5590 1157.6101 -44.1339 574 583 (K)(I)
1179.5518 1179.5945 -36.1826 406 415 (K)(I)
1493.6721 1493.6960 -15.9595 459 471 (K)(T)
1791.6320 1791.7642 -73.7487 33 48 (K)LENCSNPASMGNQDPK(Q) 1Met-ox 1Cys-am
1837.8562 1837.7849 38.8159 304 318 (R)THTGEKPYECEQCGK(A) 1Cys-am
1837.8562 1837.8213 19.0170 360 374 (R)SHTGEKPYECKECGK(S) 2Cys-am
1838.8257 1838.7260 54.2313 472 486 (R)THTGDCPFECNQCGK(A) 2Cys-am
1851.8468 1851.8006 24.9575 304 318 (R)THTGEKPYECEQCGK(A) 2Cys-am
2283.0760 2283.1078 -13.9172 152 171 (K)(K)
2283.0760 2283.0538 9.7180 416 434 (R)IHTGEKPFECNECGKTFSK(K) 1Cys-am
2784.2402 2784.3561 -41.6309 548 570 (K)SQLVIHQRSHTGEKPYECIECGK(A) 2Cys-am
2902.2721 2902.3075 -12.1722 448 471 (K)EKPYKCDECGKAFGHSSSLTYHMR(T) 1Cys-am

Click link below to search for another component.

Click individual mass to do a non-specific cleavage search.

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
897.3618	897.4253	-70.7437	371	377	(K)ECGKSFR(Y)	1Cys-am
1157.5590	1157.6101	-44.1339	574	583	(K)(I)
1179.5518	1179.5945	-36.1826	406	415	(K)(I)
1493.6721	1493.6960	-15.9595	459	471	(K)(T)
1791.6320	1791.7642	-73.7487	33	48	(K)LENCSNPASMGNQDPK(Q)	1Met-ox 1Cys-am
1837.8562	1837.7849	38.8159	304	318	(R)THTGEKPYECEQCGK(A)	1Cys-am
1837.8562	1837.8213	19.0170	360	374	(R)SHTGEKPYECKECGK(S)	2Cys-am
1838.8257	1838.7260	54.2313	472	486	(R)THTGDCPFECNQCGK(A)	2Cys-am
1851.8468	1851.8006	24.9575	304	318	(R)THTGEKPYECEQCGK(A)	2Cys-am
2283.0760	2283.1078	-13.9172	152	171	(K)(K)
2283.0760	2283.0538	9.7180	416	434	(R)IHTGEKPFECNECGKTFSK(K)	1Cys-am
2784.2402	2784.3561	-41.6309	548	570	(K)SQLVIHQRSHTGEKPYECIECGK(A)	2Cys-am
2902.2721	2902.3075	-12.1722	448	471	(K)EKPYKCDECGKAFGHSSSLTYHMR(T)	1Cys-am

The matched peptides cover 28% (170/594AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 20.8 Mean Error = -12.4422 ppm Data Tolerance = 80.7142 ppm Mean Missed Cleavages = 0.8

5. 10/53 matches (18%). 89614.9 Da, pI = 8.61. Acc. # P21580. HUMAN. Tumor necrosis factor, alpha-induced protein 3 (Putative DNA binding protein A20) (Zinc finger protein A20).

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
1179.5518 1179.5581 -5.3370 677 685 (K)CFIEAQNQR(F) 1Cys-am
1389.6959 1389.7199 -17.2173 691 701 (K)(R)
1389.6959 1389.7199 -17.2173 692 702 (R)(D)
1475.6977 1475.6841 9.2556 288 299 (K)VHFLTDPENEMK(E) 1Met-ox
1493.6721 1493.6921 -13.3889 622 632 (K)GFCTLCFIEYR(E) 2Cys-am
1640.6687 1640.6296 23.8128 775 786 (K)CNGYCNECFQFK(Q) 1Cys-am
1716.7775 1716.8267 -28.6465 288 301 (K)(L)
1838.8257 1838.8965 -38.4985 707 722 (R)(N)
1851.8468 1851.9751 -69.2869 421 439 (K)(G)
1993.9060 1994.0500 -72.2069 25 41 (R)(T)
2786.2986 2786.2462 18.8014 480 502 (R)SPGCPFTLNVQHNGFCERCHNAR(Q) 2Cys-am

Click link below to search for another component.

Click individual mass to do a non-specific cleavage search.

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
1179.5518	1179.5581	-5.3370	677	685	(K)CFIEAQNQR(F)	1Cys-am
1389.6959	1389.7199	-17.2173	691	701	(K)(R)
1389.6959	1389.7199	-17.2173	692	702	(R)(D)
1475.6977	1475.6841	9.2556	288	299	(K)VHFLTDPENEMK(E)	1Met-ox
1493.6721	1493.6921	-13.3889	622	632	(K)GFCTLCFIEYR(E)	2Cys-am
1640.6687	1640.6296	23.8128	775	786	(K)CNGYCNECFQFK(Q)	1Cys-am
1716.7775	1716.8267	-28.6465	288	301	(K)(L)
1838.8257	1838.8965	-38.4985	707	722	(R)(N)
1851.8468	1851.9751	-69.2869	421	439	(K)(G)
1993.9060	1994.0500	-72.2069	25	41	(R)(T)
2786.2986	2786.2462	18.8014	480	502	(R)SPGCPFTLNVQHNGFCERCHNAR(Q)	2Cys-am

The matched peptides cover 16% (133/790AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 18.9 Mean Error = -19.0845 ppm Data Tolerance = 63.6167 ppm Mean Missed Cleavages = 0.7