MS-Fit Search Results


.

Parameters

Database searched: SwissProt.8.17.2002
Digest Used: Trypsin
Max. # Missed Cleavages: 2
Peptide N terminus: Hydrogen
Peptide C terminus: Free Acid
Cysteine Modification: acrylamide
Instrument Name: MALDI-TOF
Sample ID (comment): H100
Minimum Matches: 4
Sort Type: Score Sort
Considered modifications: | Peptide N-terminal Gln to pyroGlu | Oxidation of M | Protein N-terminus Acetylated |
Min Parent Ion Matches: 1
MOWSE On: 1
MOWSE P Factor: 0.4

Pre Search Results

Number of entries in the database: 113193
Molecular weight search (1000 - 100000 Da) selects 106912 entries.
Full pI range: 113193 entries.
Species search ( HUMAN MOUSE ) selects 13762 entries.
Combined molecular weight, pI and species searches select 12196 entries.
Pre searches select 12196 entries.

Data Set 1 Results

MS-Fit search selects 5011 entries (results displayed for top 10 matches).

Results Summary

MOWSE
Score		#/99(%)
Masses
Matched 		%
Cov		%
TIC		Mean
Err
ppm		Data
Tol
ppm		MS-Digest Index #Protein MW (Da)/pIAccession #SpeciesProtein Name
11.811e+00924 (24)39.024.228.174.8 66018/8.2HUMANKeratin, type II cytoskeletal 1 (Cytokeratin 1) (K1) (CK 1) (67 kDa cytokeratin) (Hair alpha protein)
21.862e+00615 (15)27.015.212.489.4 96493/5.4MOUSEHeat-shock protein 105 kDa (Heat shock-related 100 kDa protein E7I) (HSP-E7I) (Heat shock 110 kDa protein) (42 degrees C-HSP)
31.139e+00614 (14)22.014.110.586.8 96866/5.3HUMANHeat-shock protein 105 kDa (Heat shock 110 kDa protein) (Antigen NY-CO-25)
42.297e+00514 (14)32.014.113.372.9 65866/8.1HUMANKeratin, type II cytoskeletal 2 epidermal (Cytokeratin 2e) (K2e) (CK 2e)
51.099e+00514 (14)23.014.134.671.0 59519/5.1HUMANKeratin, type I cytoskeletal 10 (Cytokeratin 10) (K10) (CK 10)
69.975e+00414 (14)30.014.122.082.1 80646/6.7MOUSEProtein-tyrosine phosphatase epsilon precursor (R-PTP-epsilon)
77.666e+00414 (14)17.014.123.079.0 89615/8.6HUMANTumor necrosis factor, alpha-induced protein 3 (Putative DNA binding protein A20) (Zinc finger protein A20)
85.257e+00412 (12)29.012.17.08137 57118/8.7HUMANEBP50-PDZ interactor of 64 kDa (EPI64 protein)
94.873e+00415 (15)26.015.2-0.25293.9 97623/5.6HUMANCoatomer gamma-2 subunit (Gamma-2 coat protein) (Gamma-2 COP)
102.472e+00416 (16)20.016.2-12.093.1 87799/6.7HUMANGlutaminyl-tRNA synthetase (Glutamine--tRNA ligase) (GlnRS)
45313x.x......x.x........x.x...x......xx.x.x....x...x.....xxx.x..x.....x.x........x...x..........xx.....
99000.....................x........x..x....x.........x.x.......x..x.xx.x.x......x......x.......x........
27666........x............x........x..x.............x..x............xx.x...x...........x.......xx..x....
110808..x.......x...x..x......xx......x...x..xx......x....x..............x........................x......
108237x.....x.....x..x..x.......x..x......x.....x...x..xx.......x.....................x..................
65413.....x...................x..........x.........xx......x..x...xx.......x...x........x........x...x..
42717................x....xx...xx...........x.......x.x.......x..x...x....xx......x.....................
7014........x....x...x............x..........x..x.x.............x............x.......x....x...x........
89213.x..........x......x........x......................x......x.x..x....x....x..........x...xx..xx.....
54254x.......x.x...x..........x...x.............xxx........x.....x.x........x.........xx..x.............


Detailed Results

1. 24/99 matches (24%).
Acc. #: Species: HUMAN Name: Keratin, type II cytoskeletal 1 (Cytokeratin 1) (K1) (CK 1) (67 kDa cytokeratin) (Hair alpha protein)
Index: MW: 66018 Da pI: 8.2
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
932.4516932.5053-584104171(R) (Q)
973.5072973.5318-253964030(K) (V)
1033.52181033.51665.14844920(R) (M)
1045.56391045.5754-111781852(K) (S)
1127.54461127.54073.42892981(K) (V)
1179.62481179.6010203773860(K) (H)
1201.62151201.6905-574084172(R) (Q)
1265.62501265.6378-102782880(R) (K)
1277.73461277.6337792472571(K) (N)
1277.73461277.7105194734830(K) (T)
1300.60091300.5302542582670(K) (N)
1308.70911308.6548422682772(R) (T)
1383.73581383.6909321861970(K) (V)
1475.80951475.7494412122230(K) (T)
1475.80951475.7858162002110(R) (W)
1638.94891638.8604541861991(K) (F)
1657.87081657.79344713290(R) (R)
1688.04891687.97074666821(R) (G)
1699.92661699.825160pyroGlu 4184320(K)QISNLQQSISDAEQR(G)
1716.91581716.8517374184320(K) (G)
1813.94501813.89452813301(R) (T)
1845.00301844.9466314174321(K) (G)
1994.09721993.9772602242390(R) (R)
2150.22862150.0783702242401(R) (R)
2384.13982383.9524795195490(R) (G)
2501.42362501.2524683653862(K) (H)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 39% (256/644AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

2. 15/99 matches (15%).
Acc. #: Species: MOUSE Name: Heat-shock protein 105 kDa (Heat shock-related 100 kDa protein E7I) (HSP-E7I) (Heat shock 110 kDa protein) (42 degrees C-HSP)
Index: MW: 96493 Da pI: 5.4
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1133.49471133.5591-577077150(R) (V)
1236.59021236.6952-855795891(K) (I)
1265.62501265.5472617417500(K) (K)
1308.70911308.65104485950(K) (N)
1487.78251487.77068.03323460(K) (I)
1563.81621563.80278.63753880(R) (V)
1707.86721707.9468-473173310(K) (A)
1754.94541754.8925308168312(R) (N)
1765.86161765.8067317387512(K) (V)
1765.86161765.8431111Met-ox 7417542(K)YNHIDESEMKKVEK(S)
1791.85861791.9353-434624772(R) (V)
1813.94501813.93117.62402532(K) (L)
1845.00301844.93673654691(K) (F)
1941.04851940.9718403573741(K) (G)
2182.20792182.0854561351530(K) (R)
2343.22382343.0671672762950(K) (D)
2343.22382343.1266411972170(R) (G)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 27% (232/858AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

3. 14/99 matches (14%).
Acc. #: Species: HUMAN Name: Heat-shock protein 105 kDa (Heat shock 110 kDa protein) (Antigen NY-CO-25)
Index: MW: 96866 Da pI: 5.3
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1009.47071009.4777-6.97157220(K) (L)
1133.49471133.5591-577067140(R) (M)
1236.59021236.6952-855785881(K) (I)
1265.62501265.5472617407490(K) (K)
1475.80951475.7342513613740(K) (G)
1563.81621563.80278.63753880(R) (V)
1765.86161765.8067317377502(K) (V)
1765.86161765.8431111Met-ox 7407532(K)YNHIDESEMKKVEK(S)
1765.86161765.8696-4.51Met-ox 7157281(K)MFEELGQRLQHYAK(I)
1791.85861791.9353-434624772(R) (V)
1813.94501813.93117.62402532(K) (L)
1867.96321867.95882.47918060(K) (I)
2182.20792182.0854561351530(K) (R)
2343.22382343.0671672762950(K) (D)
2369.25762369.19752554732(K) (A)
2663.55862663.4442437898112(K) (L)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 22% (191/858AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

4. 14/99 matches (14%).
Acc. #: Species: HUMAN Name: Keratin, type II cytoskeletal 2 epidermal (Cytokeratin 2e) (K2e) (CK 2e)
Index: MW: 65866 Da pI: 8.1
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
973.5072973.5318-254004070(K) (V)
1037.50761037.5267-182452530(R) (T)
1082.56261082.5999-341952031(K) (F)
1107.54121107.5435-2.13603680(K) (S)
1193.62251193.61664.93813900(K) (H)
1193.62251193.6279-4.52442531(K) (T)
1194.60581194.6119-5.02732812(K) (T)
1254.68271254.60796021340(R) (R)
1300.60091300.5812151Met-ox 1101(-)MSCQISCKSR(G)
1320.63761320.58344146610(R) (S)
1329.72701329.6399654254360(K) (G)
1329.72701329.72660.303483590(R) (A)
1475.80951475.7858162042150(R) (W)
1588.81701588.6839846106290(R) (G)
1839.03061838.91496371920(K) (G)
2384.13982384.1404-0.252542732(R) (Y)
2384.13982384.2172-324684871(R) (K)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 32% (212/645AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

5. 14/99 matches (14%).
Acc. #: Species: HUMAN Name: Keratin, type I cytoskeletal 10 (Cytokeratin 10) (K10) (CK 10)
Index: MW: 59519 Da pI: 5.1
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
932.4516932.3784791Met-ox 2862920(K)NHEEEMK(D)
993.4691993.5005-322382450(K) (Q)
1060.56711060.4733881Met-ox 2852921(K)KNHEEEMK(D)
1060.56711060.5097541Met-ox 191(-)MSVRYSSSK(H)
1090.52351090.5315-7.41481560(K) (L)
1109.50261109.4904113353430(K) (S)
1201.62151201.61773.12462560(R) (R)
1234.69021234.67968.62362451(R) (Q)
1300.60091300.59564.12862951(K) (N)
1365.68271365.6399313233330(R) (K)
1434.82511434.7705384404501(K) (S)
1493.81151493.7348513233341(R) (D)
1563.81621563.69347916350(R) (I)
1707.86721707.77275541590(K) (G)
2097.09852096.9810564234390(R) (I)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 23% (138/593AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

6. 14/99 matches (14%).
Acc. #: Species: MOUSE Name: Protein-tyrosine phosphatase epsilon precursor (R-PTP-epsilon)
Index: MW: 80646 Da pI: 6.7
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
992.5678992.5053634234300(K) (K)
1194.60581194.5544432112190(K) (M)
1300.60091300.7160-884444552(R) (V)
1434.82511434.7957216366480(R) (V)
1434.82511434.81099.95675781(R) (Q)
1475.80951475.7099674404521(K) (K)
1475.80951475.8256-112262381(R) (K)
1657.87081657.8194312752871(R) (A)
1699.92661699.84374984971(K) (V)
1754.94541754.9117191111240(K) (V)
1755.96681755.9288221Met-ox 5745872(K)QPLARQEEQVRMVR(Q)
1867.96321867.9298182602740(R) (K)
1940.06811939.9561581601742(R) (V)
2199.18302199.0136772Met-ox 3413590(R)TGTFIVIDAMMDMIHSEQK(V)
2384.13982384.1531-5.66666830(R) (V)
2717.36252717.4165-202913140(R) (F)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 30% (212/699AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

7. 14/99 matches (14%).
Acc. #: Species: HUMAN Name: Tumor necrosis factor, alpha-induced protein 3 (Putative DNA binding protein A20) (Zinc finger protein A20)
Index: MW: 89615 Da pI: 8.6
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1096.47821096.47671.31Met-ox 1741830(K)MASTDTPMAR(S)
1133.49471133.4976-2.63563640(K) (R)
1179.62481179.5581576776850(K) (F)
1201.62151201.5424667647740(R) (C)
1207.65961207.7163-4762711(R) (N)
1320.63761320.6548-137507611(R) (C)
1475.80951475.6841851Met-ox 2882990(K)VHFLTDPENEMK(E)
1493.81151493.6921806226320(K) (E)
1699.92661699.9343-4.567811(K) (K)
1716.91581716.8267522883011(K) (L)
1791.85861791.8601-0.836776901(K) (R)
1852.03031851.9751304214390(K) (G)
1867.96321867.9700-3.61Met-ox 4214390(K)LNSKPGPEGLPGMALGASR(G)
1994.09721994.05002425410(R) (T)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 17% (137/790AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

8. 12/99 matches (12%).
Acc. #: Species: HUMAN Name: EBP50-PDZ interactor of 64 kDa (EPI64 protein)
Index: MW: 57118 Da pI: 8.7
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1009.47071009.5107-401201280(R) (V)
1065.52091065.6057-803133221(K) (A)
1107.54121107.6414-904124220(R) (A)
1236.59021236.6741-681201301(R) (L)
1350.72731350.6152831Met-ox 86950(K)WLDMLNNWDK(W)
1424.77331424.6785671611710(R) (G)
1434.82511434.81814.93603701(R) (R)
1716.91581716.7452994945080(R) (-)
1930.00881930.0901-422352510(K) (V)
2150.22862150.1028593233402(K) (I)
2230.32952230.1793673413590(K) (Q)
2343.22382343.1655251291492(K) (W)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 29% (152/508AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

9. 15/99 matches (15%).
Acc. #: Species: HUMAN Name: Coatomer gamma-2 subunit (Gamma-2 coat protein) (Gamma-2 COP)
Index: MW: 97623 Da pI: 5.6
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
937.3642937.4227-623513590(K) (L)
1060.56711060.6155-464664741(R) (F)
1111.59061111.58227.61Met-ox 1431511(R)YMKQAIVDK(V)
1232.62961232.7479-964554651(K) (T)
1566.81661566.7595363944060(K) (D)
1707.86721707.8488111121262(K) (A)
1716.91581716.9431-168418561(R) (S)
1765.86161765.8828-121Met-ox 1521680(K)VSSVSSSALVSSLHMMK(I)
1845.00301845.0420-213773921(K) (K)
1930.00881929.9843134164320(R) (E)
2211.26912211.19983125432(K) (C)
2284.32472284.1616711271452(R) (Q)
2297.33652297.2691292843040(R) (Y)
2384.13982384.1994-255395600(R) (A)
2501.42362501.2366752Met-ox 941161(K)EMATISEDVIIVTSSLTKDMTGK(E)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 26% (228/871AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

10. 16/99 matches (16%).
Acc. #: Species: HUMAN Name: Glutaminyl-tRNA synthetase (Glutamine--tRNA ligase) (GlnRS)
Index: MW: 87799 Da pI: 6.7
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
932.4516932.4365166216280(K) (R)
1009.47071009.4954-243954030(K) (M)
1037.50761037.5645-554204271(R) (T)
1082.56261082.5226372Met-ox 4044121(R)MKLVMEDGK(M)
1194.60581194.6119-5.03934031(K) (M)
1234.69021234.7159-2151620(K) (L)
1383.73581383.8000-4668791(R) (K)
1424.77331424.9065-944975092(K) (D)
1427.85841427.84876.84874982(R) (I)
1657.87081657.9349-3920342(K) (E)
1716.91581716.9648-297607742(K) (V)
1755.96681755.9757-5.165792(R) (K)
1924.85381925.0245-894995151(K) (L)
2150.22862150.1102553773942(R) (F)
2182.20792182.1000492Met-ox 3773942(R)DRPMEESLLLFEAMRKGK(F)
2225.25322225.1422505395580(R) (D)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 20% (158/775AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

MS-Fit in ProteinProspector 4.0.4
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