MS-Fit Search Results

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Sample ID (comment): HSG301
Database searched: SwissProt.8.28.2000
Molecular weight search (1000 - 100000 Da) selects 83787 entries.
Full pI range: 88628 entries.
Species search ( HUMAN MOUSE ) selects 9907 entries.
Combined molecular weight, pI and species searches select 8922 entries.
Pre searches select 8922 entries.
MS-Fit search selects 1426 entries (results displayed for top 5 matches).

Parameters Used in Search

Considered modifications: | Oxidation of M | Protein N-terminus Acetylated | Acrylamide Modified Cys |

4	50.000 ppm	monoisotopic	Trypsin	4	unmodified	Hydrogen (H)	Free Acid (O H)
Min. # Peptides to Match	Peptide Mass Tolerance (+/-)	Peptide Masses are	Digest Used	Max. # Missed Cleavages	Cysteines Modified by	Peptide N terminus	Peptide C terminus

Result Summary

Rank	MOWSE Score	# (%) Masses Matched	Protein MW (Da)/pI	Species	Protein Name
1	1.57e+008	23/60 (38%)	95339.0 / 6.41	HUMAN	ELONGATION FACTOR 2 (EF-2)
2	1.9e+003	8/60 (13%)	66018.2 / 8.16	HUMAN	KERATIN, TYPE II CYTOSKELETAL 1 (CYTOKERATIN 1) (K1) (CK 1) (67 KD CYTOKERATIN) (HAIR ALPHA PROTEIN)
3	945	10/60 (16%)	54497.2 / 6.88	MOUSE	HEPARIN COFACTOR II PRECURSOR (HC-II) (PROTEASE INHIBITOR LEUSERPIN 2)
4	558	8/60 (13%)	82792.1 / 5.94	MOUSE	DIACYLGLYCEROL KINASE, ALPHA (DIGLYCERIDE KINASE) (DGK-ALPHA) (DAG KINASE ALPHA) (80 KDA DIACYLGLYCEROL KINASE)
5	360	10/60 (16%)	91547.1 / 5.36	MOUSE	DNA REPLICATION LICENSING FACTOR MCM3 (DNA POLYMERASE ALPHA HOLOENZYME-ASSOCIATED PROTEIN P1) (P1-MCM3)

Detailed Results

1. 23/60 matches (38%). 95339.0 Da, pI = 6.41. Acc. # P13639. HUMAN. ELONGATION FACTOR 2 (EF-2).

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
760.4073	760.3782	38.2578	410	415	(R)(V)
890.5186	890.5100	9.7168	499	506	(K)(V)
912.4427	912.4171	28.0494	252	258	(K)KVEDMMK(K)	2Met-ox
912.4427	912.4171	28.0494	253	259	(K)VEDMMKK(L)	2Met-ox
1013.5480	1013.5016	45.7994	240	249	(K)(A)
1055.5346	1055.5269	7.2314	392	400	(K)GPLMMYISK(M)	1Met-ox
1084.5832	1084.5501	30.4575	300	308	(K)(K)
1138.5469	1138.5744	-24.1906	273	283	(K)(K)
1138.5469	1138.5169	26.3255	639	647	(K)(K)
1307.7029	1307.6530	38.1254	21	32	(R)(S)
1308.7089	1308.6588	38.2627	677	688	(K)(E)
1392.7738	1392.7309	30.7452	728	739	(R)CLYASVLTAQPR(L)	1Cys-am
1475.7627	1475.7901	-18.5411	151	162	(R)(A)
1487.8266	1487.8586	-21.4937	499	512	(K)(N)
1615.8311	1615.7651	40.8538	482	495	(K)(V)
1799.9106	1799.8968	7.6734	786	801	(K)(S)
1962.0501	1961.9795	35.9910	768	785	(R)(A)
1977.9756	1977.9744	0.6107	768	785	(R)GHVFEESQVAGTPMFVVK(A)	1Met-ox
2037.9607	2037.9149	22.4677	369	386	(R)CELLYEGPPDDEAAMGIK(S)	1Met-ox 1Cys-am
2119.1409	2119.0534	41.3111	649	667	(K)IWCFGPDGTGPNILTDITK(G)	1Cys-am
2143.1713	2143.0784	43.3396	606	625	(K)(Q)
2144.1153	2144.1506	-16.4592	145	162	(R)(A)
2220.2134	2220.1552	26.1873	163	180	(R)(I)
2604.4251	2604.3377	33.5554	121	144	(R)VTDGALVVVDCVSGVCVQTETVLR(Q)	2Cys-am
2773.4443	2773.4486	-1.5533	457	481	(R)YVEPIEDVPCGNIVGLVGVDQFLVK(T)	1Cys-am

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The matched peptides cover 35% (302/858AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 38.3 Mean Error = 19.6309 ppm Data Tolerance = 44.1967 ppm Mean Missed Cleavages = 0.3

2. 8/60 matches (13%). 66018.2 Da, pI = 8.16. Acc. # P04264. HUMAN. KERATIN, TYPE II CYTOSKELETAL 1 (CYTOKERATIN 1) (K1) (CK 1) (67 KD CYTOKERATIN) (HAIR ALPHA PROTEIN).

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
1179.6329 1179.6010 27.0838 377 386 (K)(H)
1308.7089 1308.6548 41.3368 268 277 (R)(T)
1383.7310 1383.6909 29.0480 186 197 (K)(V)
1475.7627 1475.7858 -15.6687 200 211 (R)(W)
1475.7627 1475.7494 8.9866 212 223 (K)(T)
1487.8266 1487.8043 15.0204 240 251 (R)(L)
1716.9008 1716.8517 28.5911 418 432 (K)(G)
1994.0188 1993.9772 20.8542 224 239 (R)(R)
2384.0190 2383.9524 27.9330 519 549 (R)(G)

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m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)
1179.6329	1179.6010	27.0838	377	386	(K)(H)
1308.7089	1308.6548	41.3368	268	277	(R)(T)
1383.7310	1383.6909	29.0480	186	197	(K)(V)
1475.7627	1475.7858	-15.6687	200	211	(R)(W)
1475.7627	1475.7494	8.9866	212	223	(K)(T)
1487.8266	1487.8043	15.0204	240	251	(R)(L)
1716.9008	1716.8517	28.5911	418	432	(K)(G)
1994.0188	1993.9772	20.8542	224	239	(R)(R)
2384.0190	2383.9524	27.9330	519	549	(R)(G)

The matched peptides cover 20% (130/644AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 13.3 Mean Error = 20.3539 ppm Data Tolerance = 32.7261 ppm Mean Missed Cleavages = 0.6

3. 10/60 matches (16%). 54497.2 Da, pI = 6.88. Acc. # P49182. MOUSE. HEPARIN COFACTOR II PRECURSOR (HC-II) (PROTEASE INHIBITOR LEUSERPIN 2).

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
538.2366 538.2513 -27.2322 213 216 (R)(A)
1091.5994 1091.6101 -9.8222 381 389 (K)(S)
1179.6329 1179.6162 14.1503 208 216 (K)(A)
1307.7029 1307.6643 29.5346 360 369 (R)(E)
1392.7738 1392.7276 33.1656 172 182 (K)(K)
1979.0305 1979.0020 14.3943 400 417 (K)(H)
2119.1409 2119.1118 13.7591 3 20 (K)HPLCTLLSLITFMCIGSK(G) 2Cys-am
2144.1153 2144.1749 -27.7844 341 359 (R)KLSGMKTLEAQLTPQVVER(W) 1Met-ox
2211.1524 2211.2211 -31.0926 381 399 (K)(N)
2510.2400 2510.2675 -10.9464 255 275 (K)EALENIDPATQMLILNCIYFK(G) 1Cys-am

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m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
538.2366	538.2513	-27.2322	213	216	(R)(A)
1091.5994	1091.6101	-9.8222	381	389	(K)(S)
1179.6329	1179.6162	14.1503	208	216	(K)(A)
1307.7029	1307.6643	29.5346	360	369	(R)(E)
1392.7738	1392.7276	33.1656	172	182	(K)(K)
1979.0305	1979.0020	14.3943	400	417	(K)(H)
2119.1409	2119.1118	13.7591	3	20	(K)HPLCTLLSLITFMCIGSK(G)	2Cys-am
2144.1153	2144.1749	-27.7844	341	359	(R)KLSGMKTLEAQLTPQVVER(W)	1Met-ox
2211.1524	2211.2211	-31.0926	381	399	(K)(N)
2510.2400	2510.2675	-10.9464	255	275	(K)EALENIDPATQMLILNCIYFK(G)	1Cys-am

The matched peptides cover 26% (125/478AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 16.7 Mean Error = -0.1874 ppm Data Tolerance = 48.3747 ppm Mean Missed Cleavages = 0.8

4. 8/60 matches (13%). 82792.1 Da, pI = 5.94. Acc. # O88673. MOUSE. DIACYLGLYCEROL KINASE, ALPHA (DIGLYCERIDE KINASE) (DGK-ALPHA) (DAG KINASE ALPHA) (80 KDA DIACYLGLYCEROL KINASE).

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
782.3894 782.3983 -11.4215 543 548 (K)(N)
1962.0501 1962.0655 -7.8612 184 202 (R)AGATTVPLLVLLGMDVTMK(D) 2Met-ox
1977.9756 1978.0319 -28.4661 649 666 (R)(L)
1977.9756 1978.0180 -21.4296 669 686 (K)(T)
1994.0188 1994.0269 -4.0468 649 666 (R)LEVVGIEGAIEMGQIYTR(L) 1Met-ox
2024.9925 2024.9250 33.3153 42 58 (R)FCQGDAIGYLGFEQFMK(M) 1Cys-am
2285.2381 2285.1310 46.8538 327 347 (R)(H)
2704.2000 2704.3155 -42.7085 547 568 (R)MKNKLWYFEFATSESIFSTCKK(L) 1Met-ox
2705.2889 2705.2955 -2.4489 92 115 (K)(L)

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m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
782.3894	782.3983	-11.4215	543	548	(K)(N)
1962.0501	1962.0655	-7.8612	184	202	(R)AGATTVPLLVLLGMDVTMK(D)	2Met-ox
1977.9756	1978.0319	-28.4661	649	666	(R)(L)
1977.9756	1978.0180	-21.4296	669	686	(K)(T)
1994.0188	1994.0269	-4.0468	649	666	(R)LEVVGIEGAIEMGQIYTR(L)	1Met-ox
2024.9925	2024.9250	33.3153	42	58	(R)FCQGDAIGYLGFEQFMK(M)	1Cys-am
2285.2381	2285.1310	46.8538	327	347	(R)(H)
2704.2000	2704.3155	-42.7085	547	568	(R)MKNKLWYFEFATSESIFSTCKK(L)	1Met-ox
2705.2889	2705.2955	-2.4489	92	115	(K)(L)

The matched peptides cover 19% (143/730AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 13.3 Mean Error = -4.2459 ppm Data Tolerance = 56.7940 ppm Mean Missed Cleavages = 0.7

5. 10/60 matches (16%). 91547.1 Da, pI = 5.36. Acc. # P25206. MOUSE. DNA REPLICATION LICENSING FACTOR MCM3 (DNA POLYMERASE ALPHA HOLOENZYME-ASSOCIATED PROTEIN P1) (P1-MCM3).

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
1300.6125 1300.6425 -23.1096 82 93 (K)(Q)
1392.7738 1392.7236 36.0541 36 46 (K)(L)
1487.8266 1487.8157 7.3471 141 153 (K)(T)
2037.9607 2037.8792 39.9863 414 430 (K)MSDMDRTAIHEVMEQGR(V) 2Met-ox
2143.1713 2143.1632 3.7363 134 152 (K)(K)
2143.1713 2143.1922 -9.7912 141 158 (K)(Y)
2144.1153 2144.0805 16.2283 115 133 (R)TLTSCFLSCVVCVEGIVTK(C) 2Cys-am
2283.2180 2283.1873 13.4560 577 596 (K)(L)
2285.2381 2285.2375 0.2607 134 152 (K)CSLVRPKVVRSVHYCPATK(K) 2Cys-am
2285.2381 2285.2916 -23.4317 229 248 (K)VKPGDRIQVVGTYRCLPGKK(G) 1Cys-am
2510.2400 2510.3156 -30.1023 294 315 (K)(A)
2704.2000 2704.2015 -0.5361 14 35 (R)(V)

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m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
1300.6125	1300.6425	-23.1096	82	93	(K)(Q)
1392.7738	1392.7236	36.0541	36	46	(K)(L)
1487.8266	1487.8157	7.3471	141	153	(K)(T)
2037.9607	2037.8792	39.9863	414	430	(K)MSDMDRTAIHEVMEQGR(V)	2Met-ox
2143.1713	2143.1632	3.7363	134	152	(K)(K)
2143.1713	2143.1922	-9.7912	141	158	(K)(Y)
2144.1153	2144.0805	16.2283	115	133	(R)TLTSCFLSCVVCVEGIVTK(C)	2Cys-am
2283.2180	2283.1873	13.4560	577	596	(K)(L)
2285.2381	2285.2375	0.2607	134	152	(K)CSLVRPKVVRSVHYCPATK(K)	2Cys-am
2285.2381	2285.2916	-23.4317	229	248	(K)VKPGDRIQVVGTYRCLPGKK(G)	1Cys-am
2510.2400	2510.3156	-30.1023	294	315	(K)(A)
2704.2000	2704.2015	-0.5361	14	35	(R)(V)

The matched peptides cover 20% (168/812AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 16.7 Mean Error = 2.5081 ppm Data Tolerance = 44.3996 ppm Mean Missed Cleavages = 1.5