MS-Fit Search Results

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Sample ID (comment): HSG302
Database searched: SwissProt.8.28.2000
Molecular weight search (1000 - 100000 Da) selects 83787 entries.
Full pI range: 88628 entries.
Species search ( HUMAN MOUSE ) selects 9907 entries.
Combined molecular weight, pI and species searches select 8922 entries.
Pre searches select 8922 entries.
MS-Fit search selects 160 entries (results displayed for top 5 matches).

Parameters Used in Search

Considered modifications: | Oxidation of M | Protein N-terminus Acetylated | Acrylamide Modified Cys |

4	50.000 ppm	monoisotopic	Trypsin	4	unmodified	Hydrogen (H)	Free Acid (O H)
Min. # Peptides to Match	Peptide Mass Tolerance (+/-)	Peptide Masses are	Digest Used	Max. # Missed Cleavages	Cysteines Modified by	Peptide N terminus	Peptide C terminus

Result Summary

Rank	MOWSE Score	# (%) Masses Matched	Protein MW (Da)/pI	Species	Protein Name
1	5.26e+003	11/26 (42%)	36426.4 / 6.91	HUMAN	MALATE DEHYDROGENASE, CYTOPLASMIC
2	196	7/26 (26%)	36477.5 / 6.16	MOUSE	MALATE DEHYDROGENASE, CYTOPLASMIC
3	60.4	5/26 (19%)	66018.2 / 8.16	HUMAN	KERATIN, TYPE II CYTOSKELETAL 1 (CYTOKERATIN 1) (K1) (CK 1) (67 KD CYTOKERATIN) (HAIR ALPHA PROTEIN)
4	34	5/26 (19%)	95522.0 / 8.31	HUMAN	VOLTAGE-GATED POTASSIUM CHANNEL PROTEIN KV2.1 (DHK1)
5	22.6	6/26 (23%)	91679.6 / 9.51	HUMAN	PRO-NEUREGULIN-2 PRECURSOR (PRO-NRG2) [CONTAINS: NEUREGULIN-2 (NRG-2) (NEURAL-AND THYMUS-DERIVED ACTIVATOR FOR ERBB KINASES) (NTAK) (DIVERGENT OF NEUREGULIN 1) (DON-1)]

Detailed Results

1. 11/26 matches (42%). 36426.4 Da, pI = 6.91. Acc. # P40925. HUMAN. MALATE DEHYDROGENASE, CYTOPLASMIC.

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
643.3348	643.3415	-10.4808	1	6	(-)SEPIR(V)	Acet N
842.5223	842.5464	-28.5926	163	170	(R)(L)
1040.5148	1040.4835	30.0386	150	157	(K)ENFSCLTR(L)	1Cys-am
1164.6285	1164.6013	23.3228	221	230	(K)(G)
1385.7479	1385.7463	1.1893	80	92	(K)(R)
1393.7565	1393.7116	32.1953	299	310	(K)(E)
1401.8063	1401.7412	46.4925	80	92	(K)DLDVAILVGSMPR(R)	1Met-ox
1751.9514	1751.8856	37.5920	206	220	(K)(G)
2214.1580	2214.2280	-31.6254	122	142	(K)KSVKVIVVGNPANTNCLTASK(S)	1Cys-am
2280.2150	2280.1162	43.3354	180	199	(K)(V)
2308.2669	2308.2090	25.0978	294	312	(K)(M)

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The matched peptides cover 35% (120/334AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 42.3 Mean Error = 15.3241 ppm Data Tolerance = 56.2538 ppm Mean Missed Cleavages = 0.6

2. 7/26 matches (26%). 36477.5 Da, pI = 6.16. Acc. # P14152. MOUSE. MALATE DEHYDROGENASE, CYTOPLASMIC.

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
643.3348 643.3415 -10.4808 1 6 (-)SEPIR(V) Acet N
1040.5148 1040.4835 30.0386 150 157 (K)ENFSCLTR(L) 1Cys-am
1393.7565 1393.7116 32.1953 299 310 (K)(E)
1751.9514 1751.8856 37.5920 206 220 (K)(G)
2214.1580 2214.2280 -31.6254 122 142 (K)KSVKVIVVGNPANTNCLTASK(S) 1Cys-am
2280.2150 2280.1162 43.3354 180 199 (K)(V)
2308.2669 2308.2090 25.0978 294 312 (K)(M)

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The matched peptides cover 26% (89/334AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 26.9 Mean Error = 18.0218 ppm Data Tolerance = 55.9546 ppm Mean Missed Cleavages = 0.9

3. 5/26 matches (19%). 66018.2 Da, pI = 8.16. Acc. # P04264. HUMAN. KERATIN, TYPE II CYTOSKELETAL 1 (CYTOKERATIN 1) (K1) (CK 1) (67 KD CYTOKERATIN) (HAIR ALPHA PROTEIN).

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
1179.6394 1179.6010 32.5594 377 386 (K)(H)
1393.7565 1393.7327 17.0346 278 289 (R)(D)
1475.8057 1475.7858 13.4535 200 211 (R)(W)
1475.8057 1475.7494 38.1094 212 223 (K)(T)
2306.2360 2306.1794 24.5119 224 241 (R)(V)
2384.0488 2383.9524 40.4501 519 549 (R)(G)

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m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)
1179.6394	1179.6010	32.5594	377	386	(K)(H)
1393.7565	1393.7327	17.0346	278	289	(R)(D)
1475.8057	1475.7858	13.4535	200	211	(R)(W)
1475.8057	1475.7494	38.1094	212	223	(K)(T)
2306.2360	2306.1794	24.5119	224	241	(R)(V)
2384.0488	2383.9524	40.4501	519	549	(R)(G)

The matched peptides cover 14% (95/644AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 19.2 Mean Error = 27.6865 ppm Data Tolerance = 22.2964 ppm Mean Missed Cleavages = 0.5

4. 5/26 matches (19%). 95522.0 Da, pI = 8.31. Acc. # Q14721. HUMAN. VOLTAGE-GATED POTASSIUM CHANNEL PROTEIN KV2.1 (DHK1).

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
1179.6394 1179.6122 23.0361 829 838 (K)(V)
1416.7228 1416.7599 -26.2212 309 321 (R)(R)
1475.8057 1475.7892 11.1694 532 544 (K)MAKTQSQPILNTK(E) 1Met-ox
2280.2150 2280.2002 6.4930 472 489 (K)(T)
2705.3211 2705.3325 -4.2097 359 382 (K)(T)

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m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
1179.6394	1179.6122	23.0361	829	838	(K)(V)
1416.7228	1416.7599	-26.2212	309	321	(R)(R)
1475.8057	1475.7892	11.1694	532	544	(K)MAKTQSQPILNTK(E)	1Met-ox
2280.2150	2280.2002	6.4930	472	489	(K)(T)
2705.3211	2705.3325	-4.2097	359	382	(K)(T)

The matched peptides cover 9% (78/854AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 19.2 Mean Error = 2.0535 ppm Data Tolerance = 37.1721 ppm Mean Missed Cleavages = 1.0

5. 6/26 matches (23%). 91679.6 Da, pI = 9.51. Acc. # O14511. HUMAN. PRO-NEUREGULIN-2 PRECURSOR (PRO-NRG2) [CONTAINS: NEUREGULIN-2 (NRG-2) (NEURAL-AND THYMUS-DERIVED ACTIVATOR FOR ERBB KINASES) (NTAK) (DIVERGENT OF NEUREGULIN 1) (DON-1)].

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
842.5223 842.4848 44.4483 748 755 (R)(A)
1475.8057 1475.8130 -4.9604 432 442 (K)(Q)
2302.1627 2302.0682 41.0761 443 462 (R)QNMCPAHQNRSLANGPSHPR(L) 1Met-ox 1Cys-am
2306.2360 2306.2006 15.3497 324 344 (R)(C)
2384.0488 2384.0725 -9.9296 351 371 (K)SYCVNGGVCYYIEGINQLSCK(C) 1Cys-am
2708.2335 2708.3003 -24.6756 257 279 (K)CEAAAGNPQPSYRWFKDGKELNR(S) 1Cys-am

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m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
842.5223	842.4848	44.4483	748	755	(R)(A)
1475.8057	1475.8130	-4.9604	432	442	(K)(Q)
2302.1627	2302.0682	41.0761	443	462	(R)QNMCPAHQNRSLANGPSHPR(L)	1Met-ox 1Cys-am
2306.2360	2306.2006	15.3497	324	344	(R)(C)
2384.0488	2384.0725	-9.9296	351	371	(K)SYCVNGGVCYYIEGINQLSCK(C)	1Cys-am
2708.2335	2708.3003	-24.6756	257	279	(K)CEAAAGNPQPSYRWFKDGKELNR(S)	1Cys-am

The matched peptides cover 12% (104/850AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 23.1 Mean Error = 10.2181 ppm Data Tolerance = 56.5978 ppm Mean Missed Cleavages = 1.5