MS-Fit Search Results

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Sample ID (comment): HSG304
Database searched: SwissProt.r36
Molecular weight search (1000 - 100000 Da) selects 69977 entries.
Full pI range: 74019 entries.
Species search ( HUMAN MOUSE ) selects 8141 entries.
Combined molecular weight, pI and species searches select 7373 entries.
MS-Fit search selects 438 entries (results displayed for top 5 matches).

Considered modifications: | Oxidation of M | Protein N-terminus Acetylated | Acrylamide Modified Cys |

Min. # Peptides
to Match
4

Peptide Mass
Tolerance (+/-)
50.000 ppm

Peptide Masses
are
monoisotopic

Digest
Used
Trypsin

Max. # Missed
Cleavages
4

Cysteines
Modified by
unmodified

Peptide
N terminus
Hydrogen (H)

Peptide
C terminus
Free Acid (O H)

Input #
Peptide Masses
30

Result Summary

Rank	MOWSE Score	# (%) Masses Matched	Protein MW (Da)/pI	Species	Protein Name
1	6.76e+005	14/30 (46%)	46426.5 / 8.36	HUMAN	ARGININOSUCCINATE SYNTHASE (EC 6.3.4.5) (CITRULLINE--ASPARTATE LIGASE).
2	4.05e+004	9/30 (30%)	46584.7 / 8.36	MOUSE	ARGININOSUCCINATE SYNTHASE (EC 6.3.4.5) (CITRULLINE--ASPARTATE LIGASE).
3	147	9/30 (30%)	73535.2 / 5.06	MOUSE	HYALURONAN-MEDIATED MOTILITY RECEPTOR (HYALURONIC ACID RECEPTOR).
4	141	7/30 (23%)	67889.1 / 9.01	HUMAN	ZINC FINGER PROTEIN 132.
5	66.1	7/30 (23%)	77054.4 / 8.55	HUMAN	KRUEPPEL-RELATED ZINC FINGER PROTEIN 3 (HKR3 PROTEIN).

Detailed Results

1. 14/30 matches (46%). 46426.5 Da, pI = 8.36. Acc. # P00966. HUMAN. ARGININOSUCCINATE SYNTHASE (EC 6.3.4.5) (CITRULLINE--ASPARTATE LIGASE)..

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
741.4589	741.4412	23.9150	141	146	(K)(M)
858.5094	858.4685	47.6250	266	272	(R)(F)
905.5332	905.5209	13.5642	356	363	(K)(E)
956.4434	956.4300	13.9995	147	153	(R)(F)
972.4261	972.4249	1.1887	147	153	(R)MPEFYNR(F)	1Met-ox
1486.7898	1486.7793	7.0214	216	228	(K)(K)
1569.8041	1569.7987	3.4572	128	140	(R)FELSCYSLAPQIK(V)	1Cys-am
1604.9053	1604.8947	6.6073	87	100	(R)YLLGTSLARPCIAR(K)	1Cys-am
1659.0490	1658.9958	32.1066	349	363	(K)(E)
1781.8838	1781.9074	-13.2245	26	41	(K)(E)
2429.2045	2429.1665	15.6244	67	86	(R)(Y)
2704.3013	2704.2387	23.1528	177	199	(K)NPWSMDENLMHISYEAGILENPK(N)	1Met-ox
2720.2682	2720.2336	12.6945	177	199	(K)NPWSMDENLMHISYEAGILENPK(N)	2Met-ox
2834.4272	2834.3823	15.8295	280	304	(R)(E)

16 unmatched masses: 993.5810 1110.5940 1591.8564 1592.8071 2211.1323 2355.1787 2371.1354 2386.1227 2388.1678 2389.1760 2410.1522 2428.1806 2452.1279 2659.3006 2885.4747 2888.3340

The matched peptides cover 38% (159/412 AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

2. 9/30 matches (30%). 46584.7 Da, pI = 8.36. Acc. # P16460. MOUSE. ARGININOSUCCINATE SYNTHASE (EC 6.3.4.5) (CITRULLINE--ASPARTATE LIGASE)..

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
741.4589 741.4412 23.9150 141 146 (K)(M)
858.5094 858.4685 47.6250 266 272 (R)(F)
905.5332 905.5209 13.5642 356 363 (K)(E)
956.4434 956.4300 13.9995 147 153 (R)(F)
972.4261 972.4249 1.1887 147 153 (R)MPEFYNR(F) 1Met-ox
1604.9053 1604.8947 6.6073 87 100 (R)YLLGTSLARPCIAR(R) 1Cys-am
1781.8838 1781.9074 -13.2245 26 41 (K)(E)
2388.1678 2388.1123 23.2279 317 335 (K)FAELVYTGFWHSPECEFVR(H) 1Cys-am
2834.4272 2834.3823 15.8295 280 304 (R)(E)

21 unmatched masses: 993.5810 1110.5940 1486.7898 1569.8041 1591.8564 1592.8071 1659.0490 2211.1323 2355.1787 2371.1354 2386.1227 2389.1760 2410.1522 2428.1806 2429.2045 2452.1279 2659.3006 2704.3013 2720.2682 2885.4747 2888.3340

The matched peptides cover 24% (102/412 AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

3. 9/30 matches (30%). 73535.2 Da, pI = 5.06. Acc. # Q00547. MOUSE. HYALURONAN-MEDIATED MOTILITY RECEPTOR (HYALURONIC ACID RECEPTOR)..

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
1486.7898 1486.7654 16.3833 544 556 (R)(Q)
1591.8564 1591.8001 35.3421 148 161 (R)(L)
1592.8071 1592.7895 11.0290 611 624 (K)(E)
1604.9053 1604.8495 34.7200 565 578 (K)(L)
2355.1787 2355.1325 19.6493 483 501 (R)TAEKENVMTELTMEINKWR(L) 2Met-ox
2389.1760 2389.2224 -19.4251 463 481 (K)(R)
2452.1279 2452.0848 17.5632 611 631 (K)AFCHASKENFTPLKEGNPNCC(-) 2Cys-am
2704.3013 2704.3430 -15.4029 366 389 (K)(E)
2885.4747 2885.4885 -4.7669 600 624 (K)ALGIRHFDPSKAFCHASKENFTPLK(E) 1Cys-am

21 unmatched masses: 741.4589 858.5094 905.5332 956.4434 972.4261 993.5810 1110.5940 1569.8041 1659.0490 1781.8838 2211.1323 2371.1354 2386.1227 2388.1678 2410.1522 2428.1806 2429.2045 2659.3006 2720.2682 2834.4272 2888.3340

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
1486.7898	1486.7654	16.3833	544	556	(R)(Q)
1591.8564	1591.8001	35.3421	148	161	(R)(L)
1592.8071	1592.7895	11.0290	611	624	(K)(E)
1604.9053	1604.8495	34.7200	565	578	(K)(L)
2355.1787	2355.1325	19.6493	483	501	(R)TAEKENVMTELTMEINKWR(L)	2Met-ox
2389.1760	2389.2224	-19.4251	463	481	(K)(R)
2452.1279	2452.0848	17.5632	611	631	(K)AFCHASKENFTPLKEGNPNCC(-)	2Cys-am
2704.3013	2704.3430	-15.4029	366	389	(K)(E)
2885.4747	2885.4885	-4.7669	600	624	(K)ALGIRHFDPSKAFCHASKENFTPLK(E)	1Cys-am

The matched peptides cover 21% (134/631 AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

4. 7/30 matches (23%). 67889.1 Da, pI = 9.01. Acc. # P52740. HUMAN. ZINC FINGER PROTEIN 132..

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
905.5332 905.5070 28.9356 556 562 (R)(E)
1486.7898 1486.7477 28.3280 251 262 (K)VHTGERPFECLK(C) 1Cys-am
2410.1522 2410.1106 17.2563 565 585 (R)(K)
2429.2045 2429.1494 22.6529 167 188 (K)(L)
2452.1279 2452.1502 -9.0985 531 550 (R)VHTQERPYECIQCGKAFSER(S) 1Cys-am
2885.4747 2885.3311 49.7941 8 32 (K)DILHLAEHQGTQSEEKPYTCGACGR(D) 2Cys-am
2888.3340 2888.4372 -35.7215 551 573 (R)STLVRHQKVHTRERTYECSQCGK(L) 2Cys-am

23 unmatched masses: 741.4589 858.5094 956.4434 972.4261 993.5810 1110.5940 1569.8041 1591.8564 1592.8071 1604.9053 1659.0490 1781.8838 2211.1323 2355.1787 2371.1354 2386.1227 2388.1678 2389.1760 2428.1806 2659.3006 2704.3013 2720.2682 2834.4272

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
905.5332	905.5070	28.9356	556	562	(R)(E)
1486.7898	1486.7477	28.3280	251	262	(K)VHTGERPFECLK(C)	1Cys-am
2410.1522	2410.1106	17.2563	565	585	(R)(K)
2429.2045	2429.1494	22.6529	167	188	(K)(L)
2452.1279	2452.1502	-9.0985	531	550	(R)VHTQERPYECIQCGKAFSER(S)	1Cys-am
2885.4747	2885.3311	49.7941	8	32	(K)DILHLAEHQGTQSEEKPYTCGACGR(D)	2Cys-am
2888.3340	2888.4372	-35.7215	551	573	(R)STLVRHQKVHTRERTYECSQCGK(L)	2Cys-am

The matched peptides cover 19% (114/589 AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

5. 7/30 matches (23%). 77054.4 Da, pI = 8.55. Acc. # P10074. HUMAN. KRUEPPEL-RELATED ZINC FINGER PROTEIN 3 (HKR3 PROTEIN)..

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
1110.5940 1110.5544 35.7025 332 340 (K)(N)
1781.8838 1781.8328 28.6352 544 557 (R)HQEGRPHFCQICGK(T) 2Cys-am
2371.1354 2371.2305 -40.0788 1 20 (-)(E)
2386.1227 2386.1623 -16.5797 312 331 (R)(E)
2389.1760 2389.1691 2.8733 361 379 (R)RRMELRVHMVSHTGEMPYK(C) 2Met-ox
2389.1760 2389.0773 41.3263 380 399 (K)CSSCSQQFMQKKDLQSHMIK(L) 2Met-ox
2428.1806 2428.1246 23.0808 380 399 (K)CSSCSQQFMQKKDLQSHMIK(L) 1Cys-am
2429.2045 2429.2512 -19.2387 415 434 (K)(L)

23 unmatched masses: 741.4589 858.5094 905.5332 956.4434 972.4261 993.5810 1486.7898 1569.8041 1591.8564 1592.8071 1604.9053 1659.0490 2211.1323 2355.1787 2388.1678 2410.1522 2452.1279 2659.3006 2704.3013 2720.2682 2834.4272 2885.4747 2888.3340

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
1110.5940	1110.5544	35.7025	332	340	(K)(N)
1781.8838	1781.8328	28.6352	544	557	(R)HQEGRPHFCQICGK(T)	2Cys-am
2371.1354	2371.2305	-40.0788	1	20	(-)(E)
2386.1227	2386.1623	-16.5797	312	331	(R)(E)
2389.1760	2389.1691	2.8733	361	379	(R)RRMELRVHMVSHTGEMPYK(C)	2Met-ox
2389.1760	2389.0773	41.3263	380	399	(K)CSSCSQQFMQKKDLQSHMIK(L)	2Met-ox
2428.1806	2428.1246	23.0808	380	399	(K)CSSCSQQFMQKKDLQSHMIK(L)	1Cys-am
2429.2045	2429.2512	-19.2387	415	434	(K)(L)

The matched peptides cover 17% (122/688 AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):