MS-Fit Search Results

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Sample ID (comment): HSG305
Database searched: SwissProt.r36
Molecular weight search (1000 - 100000 Da) selects 69977 entries.
Full pI range: 74019 entries.
Species search ( HUMAN MOUSE ) selects 8141 entries.
Combined molecular weight, pI and species searches select 7373 entries.
MS-Fit search selects 525 entries (results displayed for top 5 matches).

Considered modifications: | Oxidation of M | Protein N-terminus Acetylated | Acrylamide Modified Cys |

Min. # Peptides
to Match
4

Peptide Mass
Tolerance (+/-)
50.000 ppm

Peptide Masses
are
monoisotopic

Digest
Used
Trypsin

Max. # Missed
Cleavages
4

Cysteines
Modified by
unmodified

Peptide
N terminus
Hydrogen (H)

Peptide
C terminus
Free Acid (O H)

Input #
Peptide Masses
30

Result Summary

Rank	MOWSE Score	# (%) Masses Matched	Protein MW (Da)/pI	Species	Protein Name
1	5.55e+008	21/30 (70%)	57747.0 / 7.57	HUMAN	PYRUVATE KINASE, M1 (MUSCLE) ISOZYME (EC 2.7.1.40) (CYTOSOLIC THYROID HORMONE-BINDING PROTEIN) (CTHBP) (THBP1).
2	3.03e+008	21/30 (70%)	57783.0 / 7.95	HUMAN	PYRUVATE KINASE, M2 ISOZYME (EC 2.7.1.40).
3	3.14e+006	17/30 (56%)	57756.1 / 7.42	MOUSE	PYRUVATE KINASE, M2 ISOZYME (EC 2.7.1.40).
4	176	11/30 (36%)	93488.3 / 9.35	HUMAN	ZINC FINGER PROTEIN 43 (ZINC PROTEIN HTF6).
5	150	7/30 (23%)	83674.3 / 6.73	HUMAN	PROTEIN KINASE C, EPSILON TYPE (EC 2.7.1.-) (NPKC-EPSILON).

Detailed Results

1. 21/30 matches (70%). 57747.0 Da, pI = 7.57. Acc. # P14618. HUMAN. PYRUVATE KINASE, M1 (MUSCLE) ISOZYME (EC 2.7.1.40) (CYTOSOLIC THYROID HORMONE-BINDING PROTEIN) (CTHBP) (THBP1)..

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
840.5490	840.5307	21.7104	447	454	(R)(N)
1197.6475	1197.6479	-0.3578	32	42	(R)(N)
1373.7495	1373.7211	20.6823	43	55	(R)NTGIICTIGPASR(S)	1Cys-am
1462.8629	1462.8157	32.2596	173	185	(K)(Q)
1482.7296	1482.6939	24.0795	151	161	(K)CDENILWLDYK(N)	1Cys-am
1642.7983	1642.7713	16.4361	475	488	(K)(V)
1821.8970	1821.9169	-10.9443	278	293	(R)(G)
1837.9132	1837.9118	0.7464	278	293	(R)RFDEILEASDGIMVAR(G)	1Met-ox
1859.9109	1859.9002	5.7815	230	245	(K)(K)
1875.9099	1875.8951	7.8839	230	245	(K)FGVEQDVDMVFASFIR(K)	1Met-ox
1932.0286	1931.9867	21.6794	383	398	(R)(R)
2088.1212	2088.0878	15.9798	383	399	(R)(L)
2175.1032	2175.1185	-7.0226	400	421	(R)(C)
2263.1880	2263.1657	9.8545	505	525	(K)(V)
2279.1754	2279.1607	6.4501	505	525	(K)GDVVIVLTGWRPGSGFTNTMR(V)	1Met-ox
2349.2321	2349.2666	-14.6893	207	229	(K)(F)
2391.2936	2391.2607	13.7598	504	525	(K)(V)
2407.3223	2407.2556	27.7111	504	525	(K)KGDVVIVLTGWRPGSGFTNTMR(V)	1Met-ox
2465.3370	2465.2928	17.9168	92	114	(R)(G)
2477.3504	2477.3108	16.0024	43	65	(R)NTGIICTIGPASRSVETLKEMIK(S)	1Met-ox
2477.3504	2477.3615	-4.4928	206	229	(K)(F)
2571.3680	2571.2917	29.6547	467	488	(R)GIFPVLCKDPVQEAWAEDVDLR(V)	1Cys-am

9 unmatched masses: 1815.0635 2047.9664 2278.2324 2409.2624 2421.3425 2487.3005 2570.3065 2593.3738 3019.6401

The matched peptides cover 43% (228/530 AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

2. 21/30 matches (70%). 57783.0 Da, pI = 7.95. Acc. # P14786. HUMAN. PYRUVATE KINASE, M2 ISOZYME (EC 2.7.1.40)..

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
840.5490 840.5307 21.7104 447 454 (R)(N)
1197.6475 1197.6479 -0.3578 32 42 (R)(N)
1373.7495 1373.7211 20.6823 43 55 (R)NTGIICTIGPASR(S) 1Cys-am
1462.8629 1462.8157 32.2596 173 185 (K)(Q)
1482.7296 1482.6939 24.0795 151 161 (K)CDENILWLDYK(N) 1Cys-am
1642.7983 1642.7713 16.4361 475 488 (K)(V)
1821.8970 1821.9169 -10.9443 278 293 (R)(G)
1837.9132 1837.9118 0.7464 278 293 (R)RFDEILEASDGIMVAR(G) 1Met-ox
1859.9109 1859.9002 5.7815 230 245 (K)(K)
1875.9099 1875.8951 7.8839 230 245 (K)FGVEQDVDMVFASFIR(K) 1Met-ox
1932.0286 1931.9867 21.6794 383 398 (R)(R)
2088.1212 2088.0878 15.9798 383 399 (R)(L)
2175.1032 2175.1185 -7.0226 400 421 (R)(C)
2263.1880 2263.1657 9.8545 505 525 (K)(V)
2279.1754 2279.1607 6.4501 505 525 (K)GDVVIVLTGWRPGSGFTNTMR(V) 1Met-ox
2349.2321 2349.2666 -14.6893 207 229 (K)(F)
2391.2936 2391.2607 13.7598 504 525 (K)(V)
2407.3223 2407.2556 27.7111 504 525 (K)KGDVVIVLTGWRPGSGFTNTMR(V) 1Met-ox
2465.3370 2465.2928 17.9168 92 114 (R)(G)
2477.3504 2477.3108 16.0024 43 65 (R)NTGIICTIGPASRSVETLKEMIK(S) 1Met-ox
2477.3504 2477.3615 -4.4928 206 229 (K)(F)
2571.3680 2571.3937 -10.0057 319 341 (R)AGKPVICATQMLESMIKKPRPTR(A) 1Met-ox
2571.3680 2571.2917 29.6547 467 488 (R)GIFPVLCKDPVQEAWAEDVDLR(V) 1Cys-am

9 unmatched masses: 1815.0635 2047.9664 2278.2324 2409.2624 2421.3425 2487.3005 2570.3065 2593.3738 3019.6401

The matched peptides cover 47% (251/530 AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

3. 17/30 matches (56%). 57756.1 Da, pI = 7.42. Acc. # P52480. MOUSE. PYRUVATE KINASE, M2 ISOZYME (EC 2.7.1.40)..

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
840.5490 840.5307 21.7104 447 454 (R)(N)
1373.7495 1373.7211 20.6823 43 55 (R)NTGIICTIGPASR(S) 1Cys-am
1462.8629 1462.8157 32.2596 173 185 (K)(E)
1482.7296 1482.6939 24.0795 151 161 (K)CDENILWLDYK(N) 1Cys-am
1821.8970 1821.9169 -10.9443 278 293 (R)(G)
1837.9132 1837.9118 0.7464 278 293 (R)RFDEILEASDGIMVAR(G) 1Met-ox
1859.9109 1859.9002 5.7815 230 245 (K)(K)
1875.9099 1875.8951 7.8839 230 245 (K)FGVEQDVDMVFASFIR(K) 1Met-ox
1932.0286 1931.9867 21.6794 383 398 (R)(R)
2088.1212 2088.0878 15.9798 383 399 (R)(L)
2263.1880 2263.1657 9.8545 505 525 (K)(V)
2279.1754 2279.1607 6.4501 505 525 (K)GDVVIVLTGWRPGSGFTNTMR(V) 1Met-ox
2349.2321 2349.2666 -14.6893 207 229 (K)(F)
2391.2936 2391.2607 13.7598 504 525 (K)(V)
2407.3223 2407.2556 27.7111 504 525 (K)KGDVVIVLTGWRPGSGFTNTMR(V) 1Met-ox
2409.2624 2409.2126 20.6646 316 335 (R)CNRAGKPVICSTQMLEIMIK(K) 2Met-ox 2Cys-am
2477.3504 2477.3615 -4.4928 206 229 (K)(F)

13 unmatched masses: 1197.6475 1642.7983 1815.0635 2047.9664 2175.1032 2278.2324 2421.3425 2465.3370 2487.3005 2570.3065 2571.3680 2593.3738 3019.6401

The matched peptides cover 30% (160/530 AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

4. 11/30 matches (36%). 93488.3 Da, pI = 9.35. Acc. # P28160. HUMAN. ZINC FINGER PROTEIN 43 (ZINC PROTEIN HTF6)..

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
1197.6475 1197.6124 29.2988 167 175 (K)LFKCKECGK(S) 2Cys-am
1482.7296 1482.7350 -3.6360 176 187 (K)SFCMLPHLAQHK(I) 1Cys-am
1859.9109 1859.8784 17.4927 301 315 (K)IHPGEKPYKCEECGK(A) 2Cys-am
1875.9099 1875.9461 -19.2971 497 511 (K)IHIEKKPYKCEECGK(A) 1Cys-am
2047.9664 2048.0686 -49.8955 176 192 (K)SFCMLPHLAQHKIIHTR(V) 1Met-ox
2279.1754 2279.2044 -12.7581 496 514 (K)(W)
2407.3223 2407.3125 4.1034 783 803 (K)(-)
2409.2624 2409.2423 8.3601 384 402 (K)KIHTEKKPYKCEECGKAFK(W) 2Cys-am
2421.3425 2421.2787 26.3819 496 514 (K)KIHIEKKPYKCEECGKAFK(W) 2Cys-am
2465.3370 2465.2368 40.6172 172 192 (K)ECGKSFCMLPHLAQHKIIHTR(V) 1Met-ox
2571.3680 2571.3605 2.9178 683 704 (K)LSSTLSTHKIIHTGEKPYKCEK(C) 1Cys-am

19 unmatched masses: 840.5490 1373.7495 1462.8629 1642.7983 1815.0635 1821.8970 1837.9132 1932.0286 2088.1212 2175.1032 2263.1880 2278.2324 2349.2321 2391.2936 2477.3504 2487.3005 2570.3065 2593.3738 3019.6401

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
1197.6475	1197.6124	29.2988	167	175	(K)LFKCKECGK(S)	2Cys-am
1482.7296	1482.7350	-3.6360	176	187	(K)SFCMLPHLAQHK(I)	1Cys-am
1859.9109	1859.8784	17.4927	301	315	(K)IHPGEKPYKCEECGK(A)	2Cys-am
1875.9099	1875.9461	-19.2971	497	511	(K)IHIEKKPYKCEECGK(A)	1Cys-am
2047.9664	2048.0686	-49.8955	176	192	(K)SFCMLPHLAQHKIIHTR(V)	1Met-ox
2279.1754	2279.2044	-12.7581	496	514	(K)(W)
2407.3223	2407.3125	4.1034	783	803	(K)(-)
2409.2624	2409.2423	8.3601	384	402	(K)KIHTEKKPYKCEECGKAFK(W)	2Cys-am
2421.3425	2421.2787	26.3819	496	514	(K)KIHIEKKPYKCEECGKAFK(W)	2Cys-am
2465.3370	2465.2368	40.6172	172	192	(K)ECGKSFCMLPHLAQHKIIHTR(V)	1Met-ox
2571.3680	2571.3605	2.9178	683	704	(K)LSSTLSTHKIIHTGEKPYKCEK(C)	1Cys-am

The matched peptides cover 15% (121/803 AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

5. 7/30 matches (23%). 83674.3 Da, pI = 6.73. Acc. # Q02156. HUMAN. PROTEIN KINASE C, EPSILON TYPE (EC 2.7.1.-) (NPKC-EPSILON)..

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
1642.7983 1642.7502 29.2986 112 124 (R)(V)
2047.9664 2047.8932 35.7504 60 77 (K)(K)
2047.9664 2047.9305 17.5587 172 187 (K)FMATYLRQPTYCSHCR(D) 1Cys-am
2278.2324 2278.1203 49.2275 125 145 (R)(V)
2409.2624 2409.3578 -39.5779 298 320 (R)(K)
2487.3005 2487.2513 19.7596 344 365 (R)(A)
2570.3065 2570.2381 26.5869 378 402 (R)(L)
2571.3680 2571.3558 4.7488 705 726 (R)(G)

23 unmatched masses: 840.5490 1197.6475 1373.7495 1462.8629 1482.7296 1815.0635 1821.8970 1837.9132 1859.9109 1875.9099 1932.0286 2088.1212 2175.1032 2263.1880 2279.1754 2349.2321 2391.2936 2407.3223 2421.3425 2465.3370 2477.3504 2593.3738 3019.6401

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
1642.7983	1642.7502	29.2986	112	124	(R)(V)
2047.9664	2047.8932	35.7504	60	77	(K)(K)
2047.9664	2047.9305	17.5587	172	187	(K)FMATYLRQPTYCSHCR(D)	1Cys-am
2278.2324	2278.1203	49.2275	125	145	(R)(V)
2409.2624	2409.3578	-39.5779	298	320	(R)(K)
2487.3005	2487.2513	19.7596	344	365	(R)(A)
2570.3065	2570.2381	26.5869	378	402	(R)(L)
2571.3680	2571.3558	4.7488	705	726	(R)(G)

The matched peptides cover 21% (160/737 AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):