MS-Fit Search Results

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Sample ID (comment): HSG308
Database searched: SwissProt.r36
Molecular weight search (1000 - 100000 Da) selects 69977 entries.
Full pI range: 74019 entries.
Species search ( HUMAN MOUSE ) selects 8141 entries.
Combined molecular weight, pI and species searches select 7373 entries.
MS-Fit search selects 767 entries (results displayed for top 5 matches).

Considered modifications: | Oxidation of M | Protein N-terminus Acetylated | Acrylamide Modified Cys |

Min. # Peptides
to Match
4

Peptide Mass
Tolerance (+/-)
50.000 ppm

Peptide Masses
are
monoisotopic

Digest
Used
Trypsin

Max. # Missed
Cleavages
4

Cysteines
Modified by
unmodified

Peptide
N terminus
Hydrogen (H)

Peptide
C terminus
Free Acid (O H)

Input #
Peptide Masses
40

Result Summary

Rank	MOWSE Score	# (%) Masses Matched	Protein MW (Da)/pI	Species	Protein Name
1	4.74e+005	15/40 (37%)	44596.9 / 8.30	HUMAN	PHOSPHOGLYCERATE KINASE 1 (EC 2.7.2.3) (PRIMER RECOGNITION PROTEIN 2) (PRP 2).
2	4.85e+003	9/40 (22%)	44405.5 / 7.52	MOUSE	PHOSPHOGLYCERATE KINASE 1 (EC 2.7.2.3).
3	233	5/40 (12%)	15958.7 / 8.54	MOUSE	CALCIUM-DEPENDENT PHOSPHOLIPASE A2 PRECURSOR (EC 3.1.1.4) (PHOSPHATIDYLCHOLINE 2-ACYLHYDROLASE).
4	229	4/40 (10%)	25486.5 / 9.72	HUMAN	U2 SMALL NUCLEAR RIBONUCLEOPROTEIN B".
5	176	6/40 (15%)	49167.9 / 4.71	HUMAN	KERATIN, TYPE I CYTOSKELETAL 15 (CYTOKERATIN 15) (K15) (CK 15).

Detailed Results

1. 15/40 matches (37%). 44596.9 Da, pI = 8.30. Acc. # P00558. HUMAN. PHOSPHOGLYCERATE KINASE 1 (EC 2.7.2.3) (PRIMER RECOGNITION PROTEIN 2) (PRP 2)..

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
635.3583	635.3517	10.3538	146	150	(K)(A)
1046.4972	1046.4828	13.7040	97	105	(K)DCVGPEVEK(A)	1Cys-am
1634.7818	1634.7927	-6.6861	156	170	(K)(A)
1768.9649	1768.9961	-17.6893	199	215	(K)(V)
1782.9226	1782.9536	-17.3950	106	122	(K)ACANPAAGSVILLENLR(F)	1Cys-am
1796.8216	1796.8345	-7.1787	365	381	(R)GCITIIGGGDTATCCAK(W)	3Cys-am
1966.9026	1966.9432	-20.6239	246	263	(K)(I)
1982.8900	1982.9381	-24.2613	246	263	(K)VLNNMEIGTSLFDEEGAK(I)	1Met-ox
2023.0015	2023.0388	-18.4579	279	296	(K)(T)
2087.9706	2088.0734	-49.2210	171	190	(R)AHSSMVGVNLPQKAGGFLMK(K)	1Met-ox
2105.0627	2105.0609	0.8823	332	349	(K)(G)
2121.0172	2121.0729	-26.2632	151	170	(R)(A)
2212.0762	2212.0524	10.7399	361	381	(K)ATSRGCITIIGGGDTATCCAK(W)	3Cys-am
3279.6296	3279.7121	-25.1503	56	85	(K)SVVLMSHLGRPDGVPMPDKYSLEPVAVELK(S)	1Met-ox
3295.7111	3295.7070	1.2203	56	85	(K)SVVLMSHLGRPDGVPMPDKYSLEPVAVELK(S)	2Met-ox

25 unmatched masses: 515.3570 842.5308 1648.7861 1677.8956 1684.7599 1731.4033 1744.7819 1763.8041 1772.9073 1786.8724 1790.9357 1797.8785 1890.9553 1981.8697 1993.9508 2022.0325 2046.0180 2089.0519 2123.0774 2150.0837 2285.1873 2383.9557 2706.1386 2717.0924 3265.6506

The matched peptides cover 46% (193/417 AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

2. 9/40 matches (22%). 44405.5 Da, pI = 7.52. Acc. # P09411. MOUSE. PHOSPHOGLYCERATE KINASE 1 (EC 2.7.2.3)..

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
1634.7818 1634.7927 -6.6861 156 170 (K)(A)
1768.9649 1768.9961 -17.6893 199 215 (K)(V)
1796.8216 1796.8345 -7.1787 365 381 (R)GCITIIGGGDTATCCAK(W) 3Cys-am
1982.8900 1982.9381 -24.2613 246 263 (K)(I)
2023.0015 2023.0388 -18.4579 279 296 (K)(T)
2087.9706 2088.0734 -49.2210 171 190 (R)AHSSMVGVNLPQKAGGFLMK(K) 1Met-ox
2105.0627 2105.0609 0.8823 332 349 (K)(G)
2121.0172 2121.0729 -26.2632 151 170 (R)(A)
2212.0762 2212.0524 10.7399 361 381 (K)ATSRGCITIIGGGDTATCCAK(W) 3Cys-am

31 unmatched masses: 515.3570 635.3583 842.5308 1046.4972 1648.7861 1677.8956 1684.7599 1731.4033 1744.7819 1763.8041 1772.9073 1782.9226 1786.8724 1790.9357 1797.8785 1890.9553 1966.9026 1981.8697 1993.9508 2022.0325 2046.0180 2089.0519 2123.0774 2150.0837 2285.1873 2383.9557 2706.1386 2717.0924 3265.6506 3279.6296 3295.7111

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
1634.7818	1634.7927	-6.6861	156	170	(K)(A)
1768.9649	1768.9961	-17.6893	199	215	(K)(V)
1796.8216	1796.8345	-7.1787	365	381	(R)GCITIIGGGDTATCCAK(W)	3Cys-am
1982.8900	1982.9381	-24.2613	246	263	(K)(I)
2023.0015	2023.0388	-18.4579	279	296	(K)(T)
2087.9706	2088.0734	-49.2210	171	190	(R)AHSSMVGVNLPQKAGGFLMK(K)	1Met-ox
2105.0627	2105.0609	0.8823	332	349	(K)(G)
2121.0172	2121.0729	-26.2632	151	170	(R)(A)
2212.0762	2212.0524	10.7399	361	381	(K)ATSRGCITIIGGGDTATCCAK(W)	3Cys-am

The matched peptides cover 31% (132/416 AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

3. 5/40 matches (12%). 15958.7 Da, pI = 8.54. Acc. # P97391. MOUSE. CALCIUM-DEPENDENT PHOSPHOLIPASE A2 PRECURSOR (EC 3.1.1.4) (PHOSPHATIDYLCHOLINE 2-ACYLHYDROLASE)..

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
1744.7819 1744.7001 46.9300 40 53 (K)NYGFYGCYCGWGGR(G) 2Cys-am
1768.9649 1768.9025 35.2714 107 119 (R)LCACDRKLVYCLR(R) 3Cys-am
1782.9226 1782.9293 -3.7751 107 120 (R)LCACDRKLVYCLRR(N) 1Cys-am
2212.0762 2212.0214 24.7811 121 137 (R)(-)
2717.0924 2717.1767 -30.9935 90 112 (R)YTNGLVICEHDSFCPMRLCACDR(K) 1Cys-am

35 unmatched masses: 515.3570 635.3583 842.5308 1046.4972 1634.7818 1648.7861 1677.8956 1684.7599 1731.4033 1763.8041 1772.9073 1786.8724 1790.9357 1796.8216 1797.8785 1890.9553 1966.9026 1981.8697 1982.8900 1993.9508 2022.0325 2023.0015 2046.0180 2087.9706 2089.0519 2105.0627 2121.0172 2123.0774 2150.0837 2285.1873 2383.9557 2706.1386 3265.6506 3279.6296 3295.7111

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
1744.7819	1744.7001	46.9300	40	53	(K)NYGFYGCYCGWGGR(G)	2Cys-am
1768.9649	1768.9025	35.2714	107	119	(R)LCACDRKLVYCLR(R)	3Cys-am
1782.9226	1782.9293	-3.7751	107	120	(R)LCACDRKLVYCLRR(N)	1Cys-am
2212.0762	2212.0214	24.7811	121	137	(R)(-)
2717.0924	2717.1767	-30.9935	90	112	(R)YTNGLVICEHDSFCPMRLCACDR(K)	1Cys-am

The matched peptides cover 44% (61/137 AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

4. 4/40 matches (10%). 25486.5 Da, pI = 9.72. Acc. # P08579. HUMAN. U2 SMALL NUCLEAR RIBONUCLEOPROTEIN B"..

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
1768.9649 1768.9267 21.5430 81 95 (R)(G)
2089.0519 2088.9959 26.7832 1 17 (-)(I)
2105.0627 2104.9908 34.1613 1 17 (-)MDIRPNHTIYINNMNDK(I) 1Met-ox
2121.0172 2120.9857 14.8293 1 17 (-)MDIRPNHTIYINNMNDK(I) 2Met-ox

36 unmatched masses: 515.3570 635.3583 842.5308 1046.4972 1634.7818 1648.7861 1677.8956 1684.7599 1731.4033 1744.7819 1763.8041 1772.9073 1782.9226 1786.8724 1790.9357 1796.8216 1797.8785 1890.9553 1966.9026 1981.8697 1982.8900 1993.9508 2022.0325 2023.0015 2046.0180 2087.9706 2123.0774 2150.0837 2212.0762 2285.1873 2383.9557 2706.1386 2717.0924 3265.6506 3279.6296 3295.7111

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
1768.9649	1768.9267	21.5430	81	95	(R)(G)
2089.0519	2088.9959	26.7832	1	17	(-)(I)
2105.0627	2104.9908	34.1613	1	17	(-)MDIRPNHTIYINNMNDK(I)	1Met-ox
2121.0172	2120.9857	14.8293	1	17	(-)MDIRPNHTIYINNMNDK(I)	2Met-ox

The matched peptides cover 14% (32/225 AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

5. 6/40 matches (15%). 49167.9 Da, pI = 4.71. Acc. # P19012. HUMAN. KERATIN, TYPE I CYTOSKELETAL 15 (CYTOKERATIN 15) (K15) (CK 15)..

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
1763.8041 1763.7587 25.7588 144 158 (K)(T)
1797.8785 1797.8515 14.9961 274 288 (R)(N)
1966.9026 1966.9180 -7.8366 1 19 (-)(G)
1982.8900 1982.9129 -11.5772 1 19 (-)MTTTFLQTSSSTFGGGSTR(G) 1Met-ox
2150.0837 2150.1392 -25.7944 107 124 (K)(A)
3265.6506 3265.5833 20.6161 250 279 (K)EFSSQLAGQVNVEMDAAPGVDLTRVLAEMR(E) 2Met-ox

34 unmatched masses: 515.3570 635.3583 842.5308 1046.4972 1634.7818 1648.7861 1677.8956 1684.7599 1731.4033 1744.7819 1768.9649 1772.9073 1782.9226 1786.8724 1790.9357 1796.8216 1890.9553 1981.8697 1993.9508 2022.0325 2023.0015 2046.0180 2087.9706 2089.0519 2105.0627 2121.0172 2123.0774 2212.0762 2285.1873 2383.9557 2706.1386 2717.0924 3279.6296 3295.7111

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
1763.8041	1763.7587	25.7588	144	158	(K)(T)
1797.8785	1797.8515	14.9961	274	288	(R)(N)
1966.9026	1966.9180	-7.8366	1	19	(-)(G)
1982.8900	1982.9129	-11.5772	1	19	(-)MTTTFLQTSSSTFGGGSTR(G)	1Met-ox
2150.0837	2150.1392	-25.7944	107	124	(K)(A)
3265.6506	3265.5833	20.6161	250	279	(K)EFSSQLAGQVNVEMDAAPGVDLTRVLAEMR(E)	2Met-ox

The matched peptides cover 19% (91/456 AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):