MS-Fit Search Results

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Sample ID (comment): HSG309
Database searched: SwissProt.8.28.2000
Molecular weight search (1000 - 100000 Da) selects 83787 entries.
Full pI range: 88628 entries.
Species search ( HUMAN MOUSE ) selects 9907 entries.
Combined molecular weight, pI and species searches select 8922 entries.
Pre searches select 8922 entries.
MS-Fit search selects 1184 entries (results displayed for top 5 matches).

Parameters Used in Search

Considered modifications: | Oxidation of M | Protein N-terminus Acetylated | Acrylamide Modified Cys |

4	50.000 ppm	monoisotopic	Trypsin	4	unmodified	Hydrogen (H)	Free Acid (O H)
Min. # Peptides to Match	Peptide Mass Tolerance (+/-)	Peptide Masses are	Digest Used	Max. # Missed Cleavages	Cysteines Modified by	Peptide N terminus	Peptide C terminus

Result Summary

Rank	MOWSE Score	# (%) Masses Matched	Protein MW (Da)/pI	Species	Protein Name
1	9.39e+005	15/40 (37%)	39420.4 / 8.30	HUMAN	FRUCTOSE-BISPHOSPHATE ALDOLASE A (MUSCLE-TYPE ALDOLASE)
2	2.03e+004	10/40 (25%)	39356.3 / 8.30	MOUSE	FRUCTOSE-BISPHOSPHATE ALDOLASE A (MUSCLE-TYPE ALDOLASE)
3	528	9/40 (22%)	61906.2 / 6.79	HUMAN	DIHYDROPYRIMIDINASE RELATED PROTEIN-4 (DRP-4) (ULIP4 PROTEIN)
4	232	6/40 (15%)	33850.5 / 6.38	MOUSE	H-2 CLASS I HISTOCOMPATIBILITY ANTIGEN, ALPHA CHAIN (CLONE PAG64)
5	162	9/40 (22%)	46455.2 / 9.66	HUMAN	ZINC FINGER PROTEIN 174 (AW-1)

Detailed Results

1. 15/40 matches (37%). 39420.4 Da, pI = 8.30. Acc. # P04075. HUMAN. FRUCTOSE-BISPHOSPHATE ALDOLASE A (MUSCLE-TYPE ALDOLASE).

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
1044.5724	1044.5689	3.2985	61	69	(R)(V)
1342.7255	1342.7119	10.1039	88	99	(K)(S)
1505.7914	1505.8440	-34.9280	15	28	(K)(G)
1646.8027	1646.8098	-4.2898	43	56	(K)(R)
1646.8027	1646.8098	-4.2898	44	57	(R)(F)
1675.8499	1675.8478	1.2973	244	258	(K)(R)
1691.8341	1691.8427	-5.0622	244	258	(K)FSHEEIAMATVTALR(R)	1Met-ox
1822.9609	1822.9678	-3.8155	290	304	(K)CPLLKPWALTFSYGR(A)	1Cys-am
2102.1502	2102.1109	18.6989	70	87	(R)VNPCIGGVILFHETLYQK(A)	1Cys-am
2107.1187	2107.0970	10.3053	154	173	(K)(Y)
2123.0852	2123.0919	-3.1583	154	173	(K)IGEHTPSALAIMENANVLAR(Y)	1Met-ox
2228.0413	2228.0260	6.8873	343	364	(K)(-)
2272.1211	2272.1421	-9.2482	112	134	(K)(C)
3057.5207	3057.5744	-17.5830	260	289	(R)(C)
3161.5549	3161.5876	-10.3292	216	243	(K)ALSDHHIYLEGTLLKPNMVTPGHACTQK(F)	1Met-ox 1Cys-am
3190.7445	3190.6207	38.8175	174	201	(R)YASICQQNGIVPIVEPEILPDGDHDLKR(C)	1Cys-am

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The matched peptides cover 68% (249/364AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 37.5 Mean Error = -0.2060 ppm Data Tolerance = 32.3826 ppm Mean Missed Cleavages = 0.3

2. 10/40 matches (25%). 39356.3 Da, pI = 8.30. Acc. # P05064. MOUSE. FRUCTOSE-BISPHOSPHATE ALDOLASE A (MUSCLE-TYPE ALDOLASE).

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
1044.5724 1044.5689 3.2985 61 69 (R)(V)
1342.7255 1342.7119 10.1039 88 99 (K)(S)
1505.7914 1505.8440 -34.9280 15 28 (K)(G)
1646.8027 1646.8098 -4.2898 43 56 (K)(R)
1646.8027 1646.8098 -4.2898 44 57 (R)(F)
1822.9609 1822.9678 -3.8155 290 304 (K)CPLLKPWALTFSYGR(A) 1Cys-am
2102.1502 2102.1109 18.6989 70 87 (R)VNPCIGGVILFHETLYQK(A) 1Cys-am
2107.1187 2107.0970 10.3053 154 173 (K)(Y)
2123.0852 2123.0919 -3.1583 154 173 (K)IGEHTPSALAIMENANVLAR(Y) 1Met-ox
2272.1211 2272.1421 -9.2482 112 134 (K)(C)
3190.7445 3190.6207 38.8175 174 201 (R)YASICQQNGIVPIVEPEILPDGDHDLKR(C) 1Cys-am

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The matched peptides cover 42% (154/364AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 25.0 Mean Error = 1.9540 ppm Data Tolerance = 36.8806 ppm Mean Missed Cleavages = 0.4

3. 9/40 matches (22%). 61906.2 Da, pI = 6.79. Acc. # O14531. HUMAN. DIHYDROPYRIMIDINASE RELATED PROTEIN-4 (DRP-4) (ULIP4 PROTEIN).

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
1044.5724 1044.5802 -7.4555 8 16 (K)(L)
1434.7423 1434.7680 -17.9328 459 471 (K)(K)
1646.8027 1646.8365 -20.4859 160 173 (K)(C)
1993.9436 1993.8822 30.7908 174 189 (R)CQCSDSQMYEIFSIIR(D) 1Cys-am
2123.0852 2122.9975 41.2926 76 94 (R)LQMPVLGMTPADDFCQGTK(A) 1Cys-am
2140.0387 2140.0172 10.0805 533 552 (R)(R)
2272.1211 2272.0590 27.3421 369 390 (K)(I)
2277.1979 2277.1039 41.2885 24 43 (R)(Q)
3195.5115 3195.4471 20.1702 362 390 (R)MSMVWEKCVASGKMDENEFVAVTSTNAAK(I) 2Met-ox

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Click individual mass to do a non-specific cleavage search.

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
1044.5724	1044.5802	-7.4555	8	16	(K)(L)
1434.7423	1434.7680	-17.9328	459	471	(K)(K)
1646.8027	1646.8365	-20.4859	160	173	(K)(C)
1993.9436	1993.8822	30.7908	174	189	(R)CQCSDSQMYEIFSIIR(D)	1Cys-am
2123.0852	2122.9975	41.2926	76	94	(R)LQMPVLGMTPADDFCQGTK(A)	1Cys-am
2140.0387	2140.0172	10.0805	533	552	(R)(R)
2272.1211	2272.0590	27.3421	369	390	(K)(I)
2277.1979	2277.1039	41.2885	24	43	(R)(Q)
3195.5115	3195.4471	20.1702	362	390	(R)MSMVWEKCVASGKMDENEFVAVTSTNAAK(I)	2Met-ox

The matched peptides cover 24% (140/572AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 22.5 Mean Error = 13.8989 ppm Data Tolerance = 48.3330 ppm Mean Missed Cleavages = 0.7

4. 6/40 matches (15%). 33850.5 Da, pI = 6.38. Acc. # P01895. MOUSE. H-2 CLASS I HISTOCOMPATIBILITY ANTIGEN, ALPHA CHAIN (CLONE PAG64).

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
1675.8499 1675.8992 -29.4079 23 36 (R)(T)
1993.9436 1993.9806 -18.5553 214 230 (K)(W)
2062.1615 2062.0906 34.3864 20 36 (R)(T)
2065.1062 2065.0177 42.8564 214 230 (K)YTCHVEHEGLPEPLTLR(W) 1Cys-am
2228.0413 2227.9851 25.2346 277 297 (K)GGDYALAPVSQSSDMSLPDCK(V) 1Met-ox 1Cys-am
2383.9545 2384.0545 -41.9457 69 88 (R)(T)

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Click individual mass to do a non-specific cleavage search.

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
1675.8499	1675.8992	-29.4079	23	36	(R)(T)
1993.9436	1993.9806	-18.5553	214	230	(K)(W)
2062.1615	2062.0906	34.3864	20	36	(R)(T)
2065.1062	2065.0177	42.8564	214	230	(K)YTCHVEHEGLPEPLTLR(W)	1Cys-am
2228.0413	2227.9851	25.2346	277	297	(K)GGDYALAPVSQSSDMSLPDCK(V)	1Met-ox 1Cys-am
2383.9545	2384.0545	-41.9457	69	88	(R)(T)

The matched peptides cover 25% (75/298AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 15.0 Mean Error = 2.0948 ppm Data Tolerance = 72.6532 ppm Mean Missed Cleavages = 1.2

5. 9/40 matches (22%). 46455.2 Da, pI = 9.66. Acc. # Q15697. HUMAN. ZINC FINGER PROTEIN 174 (AW-1).

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
1630.9372 1630.8562 49.6681 130 143 (K)(V)
1646.8027 1646.8511 -29.3537 130 143 (K)KPKQWVAVCMQGQK(V) 1Met-ox
1822.9609 1822.9056 30.3000 31 46 (K)(Q)
1904.9712 1905.0446 -38.5258 195 212 (K)(M)
2105.0855 2105.0012 40.0512 168 186 (R)(L)
2228.0413 2227.9436 43.8950 347 365 (R)VHTGERPYTCGECGNCFGR(Q) 2Cys-am
2277.1979 2277.1371 26.6945 110 129 (R)(K)
2284.1349 2284.1331 0.8009 108 126 (R)(A)
2284.1349 2284.1322 1.1811 149 168 (K)(D)
2284.1349 2284.1468 -5.2127 219 240 (K)ENPQQEGAKGAKPCAVSAGRSK(G) 1Cys-am
2383.9545 2384.0447 -37.8090 346 365 (K)RVHTGERPYTCGECGNCFGR(Q) 2Cys-am

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Click individual mass to do a non-specific cleavage search.

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
1630.9372	1630.8562	49.6681	130	143	(K)(V)
1646.8027	1646.8511	-29.3537	130	143	(K)KPKQWVAVCMQGQK(V)	1Met-ox
1822.9609	1822.9056	30.3000	31	46	(K)(Q)
1904.9712	1905.0446	-38.5258	195	212	(K)(M)
2105.0855	2105.0012	40.0512	168	186	(R)(L)
2228.0413	2227.9436	43.8950	347	365	(R)VHTGERPYTCGECGNCFGR(Q)	2Cys-am
2277.1979	2277.1371	26.6945	110	129	(R)(K)
2284.1349	2284.1331	0.8009	108	126	(R)(A)
2284.1349	2284.1322	1.1811	149	168	(K)(D)
2284.1349	2284.1468	-5.2127	219	240	(K)ENPQQEGAKGAKPCAVSAGRSK(G)	1Cys-am
2383.9545	2384.0447	-37.8090	346	365	(K)RVHTGERPYTCGECGNCFGR(Q)	2Cys-am

The matched peptides cover 36% (150/407AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 22.5 Mean Error = 7.4263 ppm Data Tolerance = 65.8071 ppm Mean Missed Cleavages = 1.4