MS-Fit Search Results

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Sample ID (comment): HSG310
Database searched: SwissProt.8.28.2000
Molecular weight search (1000 - 100000 Da) selects 83787 entries.
Full pI range: 88628 entries.
Species search ( HUMAN MOUSE ) selects 9907 entries.
Combined molecular weight, pI and species searches select 8922 entries.
Pre searches select 8922 entries.
MS-Fit search selects 352 entries (results displayed for top 5 matches).

Parameters Used in Search

Considered modifications: | Oxidation of M | Protein N-terminus Acetylated | Acrylamide Modified Cys |

4	50.000 ppm	monoisotopic	Trypsin	4	unmodified	Hydrogen (H)	Free Acid (O H)
Min. # Peptides to Match	Peptide Mass Tolerance (+/-)	Peptide Masses are	Digest Used	Max. # Missed Cleavages	Cysteines Modified by	Peptide N terminus	Peptide C terminus

Result Summary

Rank	MOWSE Score	# (%) Masses Matched	Protein MW (Da)/pI	Species	Protein Name
1	1.23e+003	8/40 (20%)	66018.2 / 8.16	HUMAN	KERATIN, TYPE II CYTOSKELETAL 1 (CYTOKERATIN 1) (K1) (CK 1) (67 KD CYTOKERATIN) (HAIR ALPHA PROTEIN)
2	73.8	4/40 (10%)	36488.0 / 9.53	MOUSE	DNA-3-METHYLADENINE GLYCOSYLASE (3-METHYLADENINE DNA GLYCOSIDASE) (ADPG) (3-ALKYLADENINE DNA GLYCOSYLASE) (N-METHYLPURINE-DNA GLYCOSIRASE)
3	60.5	5/40 (12%)	61987.8 / 5.14	HUMAN	KERATIN, TYPE I CYTOSKELETAL 9 (CYTOKERATIN 9) (K9) (CK 9)
4	44.8	4/40 (10%)	41038.5 / 9.06	HUMAN	CELL DIVISION PROTEIN KINASE 10 (SERINE/THREONINE-PROTEIN KINASE PISSLRE)
5	44.8	5/40 (12%)	74935.2 / 5.61	MOUSE	VITAMIN K-DEPENDENT PROTEIN S PRECURSOR

Detailed Results

1. 8/40 matches (20%). 66018.2 Da, pI = 8.16. Acc. # P04264. HUMAN. KERATIN, TYPE II CYTOSKELETAL 1 (CYTOKERATIN 1) (K1) (CK 1) (67 KD CYTOKERATIN) (HAIR ALPHA PROTEIN).

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)
515.3227	515.3306	-15.2235	404	407	(R)(L)
1179.6061	1179.6010	4.3405	377	386	(K)(H)
1475.7866	1475.7858	0.5559	200	211	(R)(W)
1475.7866	1475.7494	25.2115	212	223	(K)(T)
1487.7465	1487.8043	-38.8153	240	251	(R)(L)
1638.8605	1638.8604	0.0497	186	199	(K)(F)
1716.8839	1716.8517	18.7906	418	432	(K)(G)
1994.0029	1993.9772	12.8712	224	239	(R)(R)
2383.9901	2383.9524	15.8162	519	549	(R)(G)

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The matched peptides cover 19% (126/644AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 20.0 Mean Error = 2.6219 ppm Data Tolerance = 39.3713 ppm Mean Missed Cleavages = 0.4

2. 4/40 matches (10%). 36488.0 Da, pI = 9.53. Acc. # Q04841. MOUSE. DNA-3-METHYLADENINE GLYCOSYLASE (3-METHYLADENINE DNA GLYCOSIDASE) (ADPG) (3-ALKYLADENINE DNA GLYCOSYLASE) (N-METHYLPURINE-DNA GLYCOSIRASE).

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
1358.7269 1358.6990 20.5510 203 214 (R)(Q)
1374.7504 1374.6939 41.0877 203 214 (R)ALEPLEGLETMR(Q) 1Met-ox
1638.8605 1638.8280 19.7972 298 311 (R)(V)
2211.1222 2211.2210 -44.6734 63 81 (R)(E)

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m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
1358.7269	1358.6990	20.5510	203	214	(R)(Q)
1374.7504	1374.6939	41.0877	203	214	(R)ALEPLEGLETMR(Q)	1Met-ox
1638.8605	1638.8280	19.7972	298	311	(R)(V)
2211.1222	2211.2210	-44.6734	63	81	(R)(E)

The matched peptides cover 13% (45/333AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 10.0 Mean Error = 9.1906 ppm Data Tolerance = 74.4787 ppm Mean Missed Cleavages = 1.0

3. 5/40 matches (12%). 61987.8 Da, pI = 5.14. Acc. # P35527. HUMAN. KERATIN, TYPE I CYTOSKELETAL 9 (CYTOKERATIN 9) (K9) (CK 9).

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
1157.6101 1157.5915 16.0973 250 260 (R)(Q)
1307.6958 1307.6782 13.4556 240 249 (R)(Q)
1315.6933 1315.6858 5.6897 212 223 (K)(T)
1851.8875 1851.9275 -21.5769 321 335 (K)(N)
2705.1771 2705.1617 5.7122 63 94 (R)(G)

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m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)
1157.6101	1157.5915	16.0973	250	260	(R)(Q)
1307.6958	1307.6782	13.4556	240	249	(R)(Q)
1315.6933	1315.6858	5.6897	212	223	(K)(T)
1851.8875	1851.9275	-21.5769	321	335	(K)(N)
2705.1771	2705.1617	5.7122	63	94	(R)(G)

The matched peptides cover 12% (80/622AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 12.5 Mean Error = 3.8756 ppm Data Tolerance = 29.9272 ppm Mean Missed Cleavages = 0.4

4. 4/40 matches (10%). 41038.5 Da, pI = 9.06. Acc. # Q15131. HUMAN. CELL DIVISION PROTEIN KINASE 10 (SERINE/THREONINE-PROTEIN KINASE PISSLRE).

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
1287.6886 1287.7135 -19.3531 187 198 (R)(V)
1303.7196 1303.7084 8.5879 187 198 (R)AYGVPVKPMTPK(V) 1Met-ox
1315.6933 1315.6435 37.8101 19 29 (K)(L)
1358.7269 1358.7140 9.4570 345 357 (K)(C)

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m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
1287.6886	1287.7135	-19.3531	187	198	(R)(V)
1303.7196	1303.7084	8.5879	187	198	(R)AYGVPVKPMTPK(V)	1Met-ox
1315.6933	1315.6435	37.8101	19	29	(K)(L)
1358.7269	1358.7140	9.4570	345	357	(K)(C)

The matched peptides cover 10% (36/360AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 10.0 Mean Error = 9.1255 ppm Data Tolerance = 46.6795 ppm Mean Missed Cleavages = 0.5

5. 5/40 matches (12%). 74935.2 Da, pI = 5.61. Acc. # Q08761. MOUSE. VITAMIN K-DEPENDENT PROTEIN S PRECURSOR.

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
1058.6225 1058.5846 35.8058 465 473 (K)(H)
1638.8605 1638.8743 -8.4431 410 424 (K)(I)
1707.7937 1707.7682 14.9576 57 69 (R)ECIEELCNKEEAR(E) 2Cys-am
1851.8875 1851.8655 11.9057 201 218 (K)(N)
1994.0029 1993.9397 31.6816 201 218 (K)DLDECALKPSVCGTAVCK(N) 2Cys-am

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m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
1058.6225	1058.5846	35.8058	465	473	(K)(H)
1638.8605	1638.8743	-8.4431	410	424	(K)(I)
1707.7937	1707.7682	14.9576	57	69	(R)ECIEELCNKEEAR(E)	2Cys-am
1851.8875	1851.8655	11.9057	201	218	(K)(N)
1994.0029	1993.9397	31.6816	201	218	(K)DLDECALKPSVCGTAVCK(N)	2Cys-am

The matched peptides cover 8% (55/675AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 12.5 Mean Error = 17.1815 ppm Data Tolerance = 35.3061 ppm Mean Missed Cleavages = 0.4