MS-Fit Search Results

Press stop on your browser if you wish to abort this MS-Fit search prematurely.
Sample ID (comment): HSG311
Database searched: SwissProt.8.28.2000
Molecular weight search (1000 - 100000 Da) selects 83787 entries.
Full pI range: 88628 entries.
Species search ( HUMAN MOUSE ) selects 9907 entries.
Combined molecular weight, pI and species searches select 8922 entries.
Pre searches select 8922 entries.
MS-Fit search selects 15 entries (results displayed for top 5 matches).

Parameters Used in Search

Considered modifications: | Oxidation of M | Protein N-terminus Acetylated | Acrylamide Modified Cys |

4	50.000 ppm	monoisotopic	Trypsin	4	unmodified	Hydrogen (H)	Free Acid (O H)
Min. # Peptides to Match	Peptide Mass Tolerance (+/-)	Peptide Masses are	Digest Used	Max. # Missed Cleavages	Cysteines Modified by	Peptide N terminus	Peptide C terminus

Result Summary

Rank	MOWSE Score	# (%) Masses Matched	Protein MW (Da)/pI	Species	Protein Name
1	39.2	4/35 (11%)	47983.8 / 8.68	MOUSE	TESTIN 2 (TES2) [CONTAINS: TESTIN 1 (TES1)]
2	33.9	4/35 (11%)	6068.4 / 8.38	HUMAN	METALLOTHIONEIN-IL (MT-1L) (MT1X)
3	28.3	4/35 (11%)	6133.4 / 8.38	HUMAN	METALLOTHIONEIN-IA (MT-1A)
4	15.5	4/35 (11%)	37375.0 / 5.85	HUMAN	FOLLISTATIN PRECURSOR (FS) (ACTIVIN-BINDING PROTEIN)
5	14.6	5/35 (14%)	51755.9 / 6.64	MOUSE	HEPATOCYTE NUCLEAR FACTOR 4-ALPHA (HNF-4-ALPHA) (TRANSCRIPTION FACTOR HNF-4) (TRANSCRIPTION FACTOR 14)

Detailed Results

1. 4/35 matches (11%). 47983.8 Da, pI = 8.68. Acc. # P47226. MOUSE. TESTIN 2 (TES2) [CONTAINS: TESTIN 1 (TES1)].

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
1705.7167	1705.7462	-17.3201	405	419	(K)(R)
1721.6972	1721.7411	-25.4813	405	419	(K)FMPVEGMVFCSVECK(R)	1Met-ox
2113.8531	2113.9357	-39.0452	226	242	(K)DKSAESKKTQYSCYCCK(H)	2Cys-am
2210.9435	2210.9603	-7.5819	405	423	(K)(-)

Click link below to search for another component.

Click individual mass to do a non-specific cleavage search.

The matched peptides cover 8% (36/423AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 11.4 Mean Error = -22.3571 ppm Data Tolerance = 26.6316 ppm Mean Missed Cleavages = 1.3

2. 4/35 matches (11%). 6068.4 Da, pI = 8.38. Acc. # P80297. HUMAN. METALLOTHIONEIN-IL (MT-1L) (MT1X).

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
2114.8941 2114.8074 41.0256 1 20 (-)DPNCSCSPVGSCACAGSCK(C) 4Cys-am Acet N
2135.8003 2135.8475 -22.0621 44 61 (K)CAQGCICKGTSDKCSCCA(-) 5Cys-am
2166.7597 2166.7879 -12.9946 1 22 (-)MDPNCSCSPVGSCACAGSCKCK(E) 1Met-ox
2454.8733 2454.9863 -46.0165 23 43 (K)ECKCTSCKKSCCSCCPVGCAK(C) 4Cys-am
2454.8733 2454.9499 -31.1959 32 56 (K)(C)

Click link below to search for another component.

Click individual mass to do a non-specific cleavage search.

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
2114.8941	2114.8074	41.0256	1	20	(-)DPNCSCSPVGSCACAGSCK(C)	4Cys-am Acet N
2135.8003	2135.8475	-22.0621	44	61	(K)CAQGCICKGTSDKCSCCA(-)	5Cys-am
2166.7597	2166.7879	-12.9946	1	22	(-)MDPNCSCSPVGSCACAGSCKCK(E)	1Met-ox
2454.8733	2454.9863	-46.0165	23	43	(K)ECKCTSCKKSCCSCCPVGCAK(C)	4Cys-am
2454.8733	2454.9499	-31.1959	32	56	(K)(C)

The matched peptides cover 100% (61/61AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 11.4 Mean Error = -14.2487 ppm Data Tolerance = 66.4400 ppm Mean Missed Cleavages = 1.6

3. 4/35 matches (11%). 6133.4 Da, pI = 8.38. Acc. # P04731. HUMAN. METALLOTHIONEIN-IA (MT-1A).

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
1705.7167 1705.6663 29.5512 31 43 (K)KSCCSCCPMSCAK(C) 5Cys-am
1721.6972 1721.6612 20.9541 31 43 (K)KSCCSCCPMSCAK(C) 1Met-ox 5Cys-am
2454.8733 2454.9321 -23.9623 32 51 (K)SCCSCCPMSCAKCAQGCICK(G) 6Cys-am
2527.8782 2527.9477 -27.4601 1 22 (-)MDPNCSCATGGSCTCTGSCKCK(E) 1Met-ox 5Cys-am
2527.8782 2527.9849 -42.1963 31 51 (K)KSCCSCCPMSCAKCAQGCICK(G) 1Met-ox 5Cys-am

Click link below to search for another component.

Click individual mass to do a non-specific cleavage search.

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
1705.7167	1705.6663	29.5512	31	43	(K)KSCCSCCPMSCAK(C)	5Cys-am
1721.6972	1721.6612	20.9541	31	43	(K)KSCCSCCPMSCAK(C)	1Met-ox 5Cys-am
2454.8733	2454.9321	-23.9623	32	51	(K)SCCSCCPMSCAKCAQGCICK(G)	6Cys-am
2527.8782	2527.9477	-27.4601	1	22	(-)MDPNCSCATGGSCTCTGSCKCK(E)	1Met-ox 5Cys-am
2527.8782	2527.9849	-42.1963	31	51	(K)KSCCSCCPMSCAKCAQGCICK(G)	1Met-ox 5Cys-am

The matched peptides cover 70% (43/61AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 11.4 Mean Error = -8.6227 ppm Data Tolerance = 63.6347 ppm Mean Missed Cleavages = 1.2

4. 4/35 matches (11%). 37375.0 Da, pI = 5.85. Acc. # P19883. HUMAN. FOLLISTATIN PRECURSOR (FS) (ACTIVIN-BINDING PROTEIN).

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
1312.4919 1312.5223 -23.2133 267 278 (R)(S)
1683.7461 1683.7868 -24.1823 239 254 (K)(K)
2210.9435 2211.0143 -31.9873 93 111 (K)ETCENVDCGPGKKCRMNKK(N) 1Cys-am
2260.0251 2260.0565 -13.8655 242 261 (K)(V)

Click link below to search for another component.

Click individual mass to do a non-specific cleavage search.

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
1312.4919	1312.5223	-23.2133	267	278	(R)(S)
1683.7461	1683.7868	-24.1823	239	254	(K)(K)
2210.9435	2211.0143	-31.9873	93	111	(K)ETCENVDCGPGKKCRMNKK(N)	1Cys-am
2260.0251	2260.0565	-13.8655	242	261	(K)(V)

The matched peptides cover 15% (54/338AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 11.4 Mean Error = -23.3121 ppm Data Tolerance = 14.8443 ppm Mean Missed Cleavages = 2.3

5. 5/35 matches (14%). 51755.9 Da, pI = 6.64. Acc. # P49698. MOUSE. HEPATOCYTE NUCLEAR FACTOR 4-ALPHA (HNF-4-ALPHA) (TRANSCRIPTION FACTOR HNF-4) (TRANSCRIPTION FACTOR 14).

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
1705.7167 1705.7215 -2.8495 62 76 (K)HYGASSCDGCKGFFR(R) 1Cys-am
2114.8941 2114.9686 -35.2279 82 97 (K)NHMYSCRFSRQCVVDK(D) 2Cys-am
2130.8862 2130.9636 -36.2829 82 97 (K)NHMYSCRFSRQCVVDK(D) 1Met-ox 2Cys-am
2147.8878 2147.9867 -46.0635 58 77 (R)(S)
2260.0251 2260.0370 -5.2395 416 438 (R)(L)

Click link below to search for another component.

Click individual mass to do a non-specific cleavage search.

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
1705.7167	1705.7215	-2.8495	62	76	(K)HYGASSCDGCKGFFR(R)	1Cys-am
2114.8941	2114.9686	-35.2279	82	97	(K)NHMYSCRFSRQCVVDK(D)	2Cys-am
2130.8862	2130.9636	-36.2829	82	97	(K)NHMYSCRFSRQCVVDK(D)	1Met-ox 2Cys-am
2147.8878	2147.9867	-46.0635	58	77	(R)(S)
2260.0251	2260.0370	-5.2395	416	438	(R)(L)

The matched peptides cover 12% (59/465AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 14.3 Mean Error = -25.1327 ppm Data Tolerance = 39.4540 ppm Mean Missed Cleavages = 1.6