MS-Fit Search Results

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Sample ID (comment): HSG312
Database searched: SwissProt.8.28.2000
Molecular weight search (1000 - 100000 Da) selects 83787 entries.
Full pI range: 88628 entries.
Species search ( HUMAN MOUSE ) selects 9907 entries.
Combined molecular weight, pI and species searches select 8922 entries.
Pre searches select 8922 entries.
MS-Fit search selects 843 entries (results displayed for top 5 matches).

Parameters Used in Search

Considered modifications: | Oxidation of M | Protein N-terminus Acetylated | Acrylamide Modified Cys |

4	50.000 ppm	monoisotopic	Trypsin	4	unmodified	Hydrogen (H)	Free Acid (O H)
Min. # Peptides to Match	Peptide Mass Tolerance (+/-)	Peptide Masses are	Digest Used	Max. # Missed Cleavages	Cysteines Modified by	Peptide N terminus	Peptide C terminus

Result Summary

Rank	MOWSE Score	# (%) Masses Matched	Protein MW (Da)/pI	Species	Protein Name
1	516	9/35 (25%)	50141.3 / 9.10	HUMAN	ELONGATION FACTOR 1-ALPHA 1 (EF-1-ALPHA-1) (ELONGATION FACTOR TU) (EF-TU)
2	516	9/35 (25%)	50164.3 / 9.10	MOUSE	ELONGATION FACTOR 1-ALPHA 1 (EF-1-ALPHA-1) (ELONGATION FACTOR TU) (EF-TU)
3	85.7	7/35 (20%)	70695.9 / 5.82	MOUSE	HEAT SHOCK-RELATED 70 KD PROTEIN
4	62.3	4/35 (11%)	31660.5 / 9.03	HUMAN	INSULIN-LIKE GROWTH FACTOR BINDING PROTEIN 3 PRECURSOR (IGFBP-3) (IBP-3) (IGF-BINDING PROTEIN 3)
5	54.3	4/35 (11%)	52004.0 / 6.10	MOUSE	ANTITHROMBIN-III PRECURSOR (ATIII)

Detailed Results

1. 9/35 matches (25%). 50141.3 Da, pI = 9.10. Acc. # P04720. HUMAN. ELONGATION FACTOR 1-ALPHA 1 (EF-1-ALPHA-1) (ELONGATION FACTOR TU) (EF-TU).

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
1025.6308	1025.6108	19.5418	256	266	(K)(V)
1314.7524	1314.7422	7.7925	135	146	(R)(Q)
1404.7560	1404.7276	20.2243	85	96	(K)(D)
1404.7560	1404.7963	-28.7150	372	382	(K)(S)
2515.3912	2515.3846	2.6532	267	290	(R)(S)
2531.4461	2531.3795	26.3190	267	290	(R)VETGVLKPGMVVTFAPVNVTTEVK(S)	1Met-ox
3009.4401	3009.4160	7.9833	396	423	(K)SGDAAIVDMVPGKPMCVESFSDYPPLGR(F)	1Cys-am
3025.4861	3025.4109	24.8378	396	423	(K)SGDAAIVDMVPGKPMCVESFSDYPPLGR(F)	1Met-ox 1Cys-am
3036.5791	3036.4876	30.1308	155	179	(K)MDSTEPPYSQKRYEEIVKEVSTYIK(K)	1Met-ox
3041.4246	3041.4059	6.1787	396	423	(K)SGDAAIVDMVPGKPMCVESFSDYPPLGR(F)	2Met-ox 1Cys-am

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The matched peptides cover 26% (123/462AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 25.7 Mean Error = 11.6946 ppm Data Tolerance = 34.2212 ppm Mean Missed Cleavages = 0.6

2. 9/35 matches (25%). 50164.3 Da, pI = 9.10. Acc. # P10126. MOUSE. ELONGATION FACTOR 1-ALPHA 1 (EF-1-ALPHA-1) (ELONGATION FACTOR TU) (EF-TU).

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
1025.6308 1025.6108 19.5418 256 266 (K)(V)
1314.7524 1314.7422 7.7925 135 146 (R)(Q)
1404.7560 1404.7276 20.2243 85 96 (K)(D)
1404.7560 1404.7963 -28.7150 372 382 (K)(S)
2515.3912 2515.3846 2.6532 267 290 (R)(S)
2531.4461 2531.3795 26.3190 267 290 (R)VETGVLKPGMVVTFAPVNVTTEVK(S) 1Met-ox
3009.4401 3009.4160 7.9833 396 423 (K)SGDAAIVDMVPGKPMCVESFSDYPPLGR(F) 1Cys-am
3025.4861 3025.4109 24.8378 396 423 (K)SGDAAIVDMVPGKPMCVESFSDYPPLGR(F) 1Met-ox 1Cys-am
3036.5791 3036.4876 30.1308 155 179 (K)MDSTEPPYSQKRYEEIVKEVSTYIK(K) 1Met-ox
3041.4246 3041.4059 6.1787 396 423 (K)SGDAAIVDMVPGKPMCVESFSDYPPLGR(F) 2Met-ox 1Cys-am

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The matched peptides cover 26% (123/462AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 25.7 Mean Error = 11.6946 ppm Data Tolerance = 34.2212 ppm Mean Missed Cleavages = 0.6

3. 7/35 matches (20%). 70695.9 Da, pI = 5.82. Acc. # P16627. MOUSE. HEAT SHOCK-RELATED 70 KD PROTEIN.

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
1314.7524 1314.7606 -6.2360 253 263 (K)(R)
1404.7560 1404.6867 49.3255 600 611 (K)(L)
1475.7383 1475.7238 9.8088 600 611 (K)ELENMCNPIITK(L) 1Cys-am
1487.7589 1487.7018 38.3806 39 51 (R)(L)
2283.2058 2283.1733 14.2202 174 195 (R)(H)
2348.2275 2348.1882 16.7242 111 130 (K)GEKKAFYPEEISSMVLTKMK(E) 2Met-ox
2515.3912 2515.3230 27.1184 571 591 (K)KILDKCNEVLSWLEANQLAEK(D) 1Cys-am

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Click individual mass to do a non-specific cleavage search.

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
1314.7524	1314.7606	-6.2360	253	263	(K)(R)
1404.7560	1404.6867	49.3255	600	611	(K)(L)
1475.7383	1475.7238	9.8088	600	611	(K)ELENMCNPIITK(L)	1Cys-am
1487.7589	1487.7018	38.3806	39	51	(R)(L)
2283.2058	2283.1733	14.2202	174	195	(R)(H)
2348.2275	2348.1882	16.7242	111	130	(K)GEKKAFYPEEISSMVLTKMK(E)	2Met-ox
2515.3912	2515.3230	27.1184	571	591	(K)KILDKCNEVLSWLEANQLAEK(D)	1Cys-am

The matched peptides cover 15% (99/641AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 20.0 Mean Error = 21.3345 ppm Data Tolerance = 37.1695 ppm Mean Missed Cleavages = 1.3

4. 4/35 matches (11%). 31660.5 Da, pI = 9.03. Acc. # P17936. HUMAN. INSULIN-LIKE GROWTH FACTOR BINDING PROTEIN 3 PRECURSOR (IGFBP-3) (IBP-3) (IGF-BINDING PROTEIN 3).

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
1487.7589 1487.7090 33.5336 145 159 (R)(V)
1779.9978 1779.9250 40.9403 47 63 (R)ALAQCAPPPAVCAELVR(E) 1Cys-am
2348.2275 2348.1208 45.4331 260 280 (R)(E)
2717.1434 2717.2703 -46.7292 268 291 (K)(-)

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Click individual mass to do a non-specific cleavage search.

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
1487.7589	1487.7090	33.5336	145	159	(R)(V)
1779.9978	1779.9250	40.9403	47	63	(R)ALAQCAPPPAVCAELVR(E)	1Cys-am
2348.2275	2348.1208	45.4331	260	280	(R)(E)
2717.1434	2717.2703	-46.7292	268	291	(K)(-)

The matched peptides cover 21% (64/291AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 11.4 Mean Error = 18.2944 ppm Data Tolerance = 87.2517 ppm Mean Missed Cleavages = 1.0

5. 4/35 matches (11%). 52004.0 Da, pI = 6.10. Acc. # P32261. MOUSE. ANTITHROMBIN-III PRECURSOR (ATIII).

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
1363.7174 1363.7408 -17.1328 447 458 (R)(V)
2299.1390 2299.1280 4.7674 104 124 (K)(L)
2531.4461 2531.4125 13.2766 233 255 (K)(G)
3036.5791 3036.5181 20.0821 134 158 (K)(L)

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m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)
1363.7174	1363.7408	-17.1328	447	458	(R)(V)
2299.1390	2299.1280	4.7674	104	124	(K)(L)
2531.4461	2531.4125	13.2766	233	255	(K)(G)
3036.5791	3036.5181	20.0821	134	158	(K)(L)

The matched peptides cover 17% (81/465AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 11.4 Mean Error = 5.2483 ppm Data Tolerance = 32.3654 ppm Mean Missed Cleavages = 0.5