MS-Fit Search Results

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Sample ID (comment): HSG313
Database searched: SwissProt.8.28.2000
Molecular weight search (1000 - 100000 Da) selects 83787 entries.
Full pI range: 88628 entries.
Species search ( HUMAN MOUSE ) selects 9907 entries.
Combined molecular weight, pI and species searches select 8922 entries.
Pre searches select 8922 entries.
MS-Fit search selects 464 entries (results displayed for top 5 matches).

Parameters Used in Search

Considered modifications: | Oxidation of M | Protein N-terminus Acetylated | Acrylamide Modified Cys |

4	50.000 ppm	monoisotopic	Trypsin	4	unmodified	Hydrogen (H)	Free Acid (O H)
Min. # Peptides to Match	Peptide Mass Tolerance (+/-)	Peptide Masses are	Digest Used	Max. # Missed Cleavages	Cysteines Modified by	Peptide N terminus	Peptide C terminus

Result Summary

Rank	MOWSE Score	# (%) Masses Matched	Protein MW (Da)/pI	Species	Protein Name
1	340	10/30 (33%)	60045.4 / 8.09	HUMAN	KERATIN, TYPE II CYTOSKELETAL 6A (CYTOKERATIN 6A) (CK 6A) (K6A KERATIN)
2	339	10/30 (33%)	60200.6 / 8.09	HUMAN	KERATIN, TYPE II CYTOSKELETAL 6C (CYTOKERATIN 6C) (CK 6C) (K6C KERATIN)
3	229	7/30 (23%)	42468.7 / 5.29	HUMAN	KERATIN, TYPE II CYTOSKELETAL 6D (CYTOKERATIN 6D) (CK 6D) (K6D KERATIN)
4	222	9/30 (30%)	59999.4 / 8.09	HUMAN	KERATIN, TYPE II CYTOSKELETAL 6B (CYTOKERATIN 6B) (CK 6B) (K6B KERATIN)
5	200	9/30 (30%)	60223.7 / 8.09	HUMAN	KERATIN, TYPE II CYTOSKELETAL 6E (CYTOKERATIN 6E) (CK 6E) (K6E KERATIN)

Detailed Results

1. 10/30 matches (33%). 60045.4 Da, pI = 8.09. Acc. # P02538. HUMAN. KERATIN, TYPE II CYTOSKELETAL 6A (CYTOKERATIN 6A) (CK 6A) (K6A KERATIN).

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
878.4294	878.4583	-32.9241	1	9	(-)ASTSTTIR(S)	Acet N
1107.5586	1107.5435	13.6653	339	347	(K)(S)
1165.5928	1165.5853	6.3952	360	369	(K)(H)
1263.7368	1263.6949	33.1723	456	466	(K)(K)
1455.6788	1455.6868	-5.5100	348	359	(R)(Y)
1890.9544	1890.9714	-8.9703	208	222	(R)(R)
2047.0719	2047.0725	-0.2997	208	223	(R)(Q)
2151.1100	2151.1926	-38.4181	165	182	(R)(F)
2350.1480	2350.1825	-14.6854	233	252	(R)(Y)
3042.4377	3042.5458	-35.5314	261	287	(R)TAAENEFVTLKKDVDAAYMNKVELQAK(A)	1Met-ox

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Click individual mass to do a non-specific cleavage search.

The matched peptides cover 23% (132/564AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 33.3 Mean Error = -8.3106 ppm Data Tolerance = 46.1224 ppm Mean Missed Cleavages = 1.1

2. 10/30 matches (33%). 60200.6 Da, pI = 8.09. Acc. # P48666. HUMAN. KERATIN, TYPE II CYTOSKELETAL 6C (CYTOKERATIN 6C) (CK 6C) (K6C KERATIN).

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
878.4294 878.4583 -32.9241 1 9 (-)ASTSTTIR(S) Acet N
1107.5586 1107.5435 13.6653 339 347 (K)(S)
1165.5928 1165.5853 6.3952 360 369 (K)(H)
1263.7368 1263.6949 33.1723 456 466 (K)(K)
1455.6788 1455.6868 -5.5100 348 359 (R)(Y)
1890.9544 1890.9714 -8.9703 208 222 (R)(R)
2047.0719 2047.0725 -0.2997 208 223 (R)(Q)
2151.1100 2151.1926 -38.4181 165 182 (R)(F)
2350.1480 2350.1825 -14.6854 233 252 (R)(Y)
3042.4377 3042.5458 -35.5314 261 287 (R)TAAENEFVTLKKDVDAAYMNKVELQAK(A) 1Met-ox

Click link below to search for another component.

Click individual mass to do a non-specific cleavage search.

The matched peptides cover 23% (132/564AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 33.3 Mean Error = -8.3106 ppm Data Tolerance = 46.1224 ppm Mean Missed Cleavages = 1.1

3. 7/30 matches (23%). 42468.7 Da, pI = 5.29. Acc. # P48667. HUMAN. KERATIN, TYPE II CYTOSKELETAL 6D (CYTOKERATIN 6D) (CK 6D) (K6D KERATIN).

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
1107.5586 1107.5435 13.6653 159 167 (K)(S)
1165.5928 1165.5853 6.3952 180 189 (K)(H)
1263.7368 1263.6949 33.1723 276 286 (K)(K)
1455.6788 1455.6868 -5.5100 168 179 (R)(Y)
1890.9544 1890.9714 -8.9703 28 42 (R)(R)
2047.0719 2047.0725 -0.2997 28 43 (R)(Q)
3042.4377 3042.5458 -35.5314 81 107 (R)TAAENEFVTLKKDVDAAYMNKVELQAK(A) 1Met-ox

Click link below to search for another component.

Click individual mass to do a non-specific cleavage search.

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
1107.5586	1107.5435	13.6653	159	167	(K)(S)
1165.5928	1165.5853	6.3952	180	189	(K)(H)
1263.7368	1263.6949	33.1723	276	286	(K)(K)
1455.6788	1455.6868	-5.5100	168	179	(R)(Y)
1890.9544	1890.9714	-8.9703	28	42	(R)(R)
2047.0719	2047.0725	-0.2997	28	43	(R)(Q)
3042.4377	3042.5458	-35.5314	81	107	(R)TAAENEFVTLKKDVDAAYMNKVELQAK(A)	1Met-ox

The matched peptides cover 22% (85/384AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 23.3 Mean Error = 0.4174 ppm Data Tolerance = 42.4281 ppm Mean Missed Cleavages = 0.7

4. 9/30 matches (30%). 59999.4 Da, pI = 8.09. Acc. # P04259. HUMAN. KERATIN, TYPE II CYTOSKELETAL 6B (CYTOKERATIN 6B) (CK 6B) (K6B KERATIN).

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
878.4294 878.4583 -32.9241 1 9 (-)ASTSTTIR(S) Acet N
1107.5586 1107.5435 13.6653 339 347 (K)(S)
1165.5928 1165.5853 6.3952 360 369 (K)(H)
1263.7368 1263.6949 33.1723 456 466 (K)(K)
1455.6788 1455.6868 -5.5100 348 359 (R)(Y)
1890.9544 1890.9714 -8.9703 208 222 (R)(R)
2047.0719 2047.0725 -0.2997 208 223 (R)(Q)
2151.1100 2151.1926 -38.4181 165 182 (R)(F)
3042.4377 3042.5458 -35.5314 261 287 (R)TAAENEFVTLKKDVDAAYMNKVELQAK(A) 1Met-ox

Click link below to search for another component.

Click individual mass to do a non-specific cleavage search.

The matched peptides cover 19% (112/564AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 30.0 Mean Error = -7.6023 ppm Data Tolerance = 48.6889 ppm Mean Missed Cleavages = 0.9

5. 9/30 matches (30%). 60223.7 Da, pI = 8.09. Acc. # P48668. HUMAN. KERATIN, TYPE II CYTOSKELETAL 6E (CYTOKERATIN 6E) (CK 6E) (K6E KERATIN).

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
878.4294 878.4583 -32.9241 1 9 (-)ASTSTTIR(S) Acet N
1107.5586 1107.5435 13.6653 339 347 (K)(S)
1165.5928 1165.5853 6.3952 360 369 (K)(H)
1263.7368 1263.6949 33.1723 456 466 (K)(K)
1455.6788 1455.6868 -5.5100 348 359 (R)(Y)
1890.9544 1890.9714 -8.9703 208 222 (R)(R)
2047.0719 2047.0725 -0.2997 208 223 (R)(Q)
2151.1100 2151.1926 -38.4181 165 182 (R)(F)
3042.4377 3042.5458 -35.5314 261 287 (R)TAAENEFVTLKKDVDAAYMNKVELQAK(A) 1Met-ox

Click link below to search for another component.

Click individual mass to do a non-specific cleavage search.

The matched peptides cover 19% (112/564AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 30.0 Mean Error = -7.6023 ppm Data Tolerance = 48.6889 ppm Mean Missed Cleavages = 0.9