MS-Fit Search Results

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Sample ID (comment): HSG314
Database searched: SwissProt.8.28.2000
Molecular weight search (1000 - 100000 Da) selects 83787 entries.
Full pI range: 88628 entries.
Species search ( HUMAN MOUSE ) selects 9907 entries.
Combined molecular weight, pI and species searches select 8922 entries.
Pre searches select 8922 entries.
MS-Fit search selects 428 entries (results displayed for top 5 matches).

Parameters Used in Search

Considered modifications: | Oxidation of M | Protein N-terminus Acetylated | Acrylamide Modified Cys |

4	50.000 ppm	monoisotopic	Trypsin	4	unmodified	Hydrogen (H)	Free Acid (O H)
Min. # Peptides to Match	Peptide Mass Tolerance (+/-)	Peptide Masses are	Digest Used	Max. # Missed Cleavages	Cysteines Modified by	Peptide N terminus	Peptide C terminus

Result Summary

Rank	MOWSE Score	# (%) Masses Matched	Protein MW (Da)/pI	Species	Protein Name
1	6.42e+005	15/40 (37%)	34196.5 / 9.27	MOUSE	HETEROGENEOUS NUCLEAR RIBONUCLEOPROTEIN A1 (HELIX-DESTABILIZING PROTEIN) (SINGLE-STRAND BINDING PROTEIN) (HNRNP CORE PROTEIN A1) (HDP-1) (TOPOISOMERASE-INHIBITOR SUPPRESSED)
2	5.65e+005	15/40 (37%)	38846.1 / 9.26	HUMAN	HETEROGENEOUS NUCLEAR RIBONUCLEOPROTEIN A1 (HELIX-DESTABILIZING PROTEIN) (SINGLE-STRAND BINDING PROTEIN) (HNRNP CORE PROTEIN A1)
3	565	5/40 (12%)	51295.6 / 4.88	HUMAN	GA BINDING PROTEIN ALPHA CHAIN (GABP-ALPHA SUBUNIT) (TRANSCRIPTION FACTOR E4TF1-60) (NUCLEAR RESPIRATORY FACTOR-2 SUBUNIT ALPHA)
4	232	6/40 (15%)	66018.2 / 8.16	HUMAN	KERATIN, TYPE II CYTOSKELETAL 1 (CYTOKERATIN 1) (K1) (CK 1) (67 KD CYTOKERATIN) (HAIR ALPHA PROTEIN)
5	220	8/40 (20%)	91679.6 / 9.51	HUMAN	PRO-NEUREGULIN-2 PRECURSOR (PRO-NRG2) [CONTAINS: NEUREGULIN-2 (NRG-2) (NEURAL-AND THYMUS-DERIVED ACTIVATOR FOR ERBB KINASES) (NTAK) (DIVERGENT OF NEUREGULIN 1) (DON-1)]

Detailed Results

1. 15/40 matches (37%). 34196.5 Da, pI = 9.27. Acc. # P49312. MOUSE. HETEROGENEOUS NUCLEAR RIBONUCLEOPROTEIN A1 (HELIX-DESTABILIZING PROTEIN) (SINGLE-STRAND BINDING PROTEIN) (HNRNP CORE PROTEIN A1) (HDP-1) (TOPOISOMERASE-INHIBITOR SUPPRESSED).

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
1049.4319	1049.4580	-24.8653	123	130	(R)(I)
1234.6282	1234.6353	-5.7309	131	140	(K)IEVIEIMTDR(G)	1Met-ox
1299.6560	1299.6545	1.1918	4	14	(K)(K)
1437.7116	1437.7450	-23.2687	93	105	(R)(K)
1628.7716	1628.7821	-6.4381	285	300	(R)(N)
1694.6681	1694.7006	-19.2211	301	318	(R)(R)
1699.7496	1699.7604	-6.3313	147	161	(R)(I)
1784.8783	1784.9071	-16.0977	16	31	(K)(S)
1879.9486	1879.9666	-9.5935	107	122	(K)(D)
1912.9900	1913.0020	-6.3068	15	31	(R)(S)
1979.9086	1979.9108	-1.0929	32	47	(R)SHFEQWGTLTDCVVMR(D)	1Cys-am
1995.9476	1995.9057	20.9782	32	47	(R)SHFEQWGTLTDCVVMR(D)	1Met-ox 1Cys-am
2195.9922	2195.9634	13.1179	114	130	(K)(I)
2510.2123	2510.2138	-0.6121	56	78	(R)(V)
2526.2665	2526.2087	22.8552	56	78	(R)GFGFVTYATVEEVDAAMNARPHK(V)	1Met-ox

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The matched peptides cover 50% (163/320AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 37.5 Mean Error = -4.0943 ppm Data Tolerance = 28.8867 ppm Mean Missed Cleavages = 0.3

2. 15/40 matches (37%). 38846.1 Da, pI = 9.26. Acc. # P09651. HUMAN. HETEROGENEOUS NUCLEAR RIBONUCLEOPROTEIN A1 (HELIX-DESTABILIZING PROTEIN) (SINGLE-STRAND BINDING PROTEIN) (HNRNP CORE PROTEIN A1).

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
1049.4319 1049.4580 -24.8653 123 130 (R)(I)
1234.6282 1234.6353 -5.7309 131 140 (K)IEVIEIMTDR(G) 1Met-ox
1299.6560 1299.6545 1.1918 4 14 (K)(K)
1437.7116 1437.7450 -23.2687 93 105 (R)(K)
1628.7716 1628.7821 -6.4381 337 352 (R)(N)
1694.6681 1694.7006 -19.2211 353 370 (R)(R)
1699.7496 1699.7604 -6.3313 147 161 (R)(I)
1784.8783 1784.9071 -16.0977 16 31 (K)(S)
1879.9486 1879.9666 -9.5935 107 122 (K)(D)
1912.9900 1913.0020 -6.3068 15 31 (R)(S)
1979.9086 1979.9108 -1.0929 32 47 (R)SHFEQWGTLTDCVVMR(D) 1Cys-am
1995.9476 1995.9057 20.9782 32 47 (R)SHFEQWGTLTDCVVMR(D) 1Met-ox 1Cys-am
2195.9922 2195.9634 13.1179 114 130 (K)(I)
2510.2123 2510.2138 -0.6121 56 78 (R)(V)
2526.2665 2526.2087 22.8552 56 78 (R)GFGFVTYATVEEVDAAMNARPHK(V) 1Met-ox

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Click individual mass to do a non-specific cleavage search.

The matched peptides cover 43% (163/372AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 37.5 Mean Error = -4.0943 ppm Data Tolerance = 28.8867 ppm Mean Missed Cleavages = 0.3

3. 5/40 matches (12%). 51295.6 Da, pI = 4.88. Acc. # Q06546. HUMAN. GA BINDING PROTEIN ALPHA CHAIN (GABP-ALPHA SUBUNIT) (TRANSCRIPTION FACTOR E4TF1-60) (NUCLEAR RESPIRATORY FACTOR-2 SUBUNIT ALPHA).

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
1307.6463 1307.6959 -37.9920 405 416 (K)(L)
1628.7716 1628.7379 20.7289 221 233 (R)(V)
1699.7496 1699.7750 -14.9231 221 233 (R)ELCSLNQEDFFQR(V) 1Cys-am
1912.9900 1912.9326 29.9724 204 220 (K)(E)
2510.2123 2510.2019 4.1227 59 79 (R)LQCSLDAHEICLQDIQLDPER(S) 1Cys-am

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m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
1307.6463	1307.6959	-37.9920	405	416	(K)(L)
1628.7716	1628.7379	20.7289	221	233	(R)(V)
1699.7496	1699.7750	-14.9231	221	233	(R)ELCSLNQEDFFQR(V)	1Cys-am
1912.9900	1912.9326	29.9724	204	220	(K)(E)
2510.2123	2510.2019	4.1227	59	79	(R)LQCSLDAHEICLQDIQLDPER(S)	1Cys-am

The matched peptides cover 13% (63/454AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 12.5 Mean Error = 0.3818 ppm Data Tolerance = 54.8670 ppm Mean Missed Cleavages = 0.0

4. 6/40 matches (15%). 66018.2 Da, pI = 8.16. Acc. # P04264. HUMAN. KERATIN, TYPE II CYTOSKELETAL 1 (CYTOKERATIN 1) (K1) (CK 1) (67 KD CYTOKERATIN) (HAIR ALPHA PROTEIN).

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
1179.5823 1179.6010 -15.7976 377 386 (K)(H)
1340.6354 1340.6698 -25.6549 365 376 (K)(Y)
1475.7534 1475.7858 -21.9650 200 211 (R)(W)
1475.7534 1475.7494 2.6901 212 223 (K)(T)
1993.9598 1993.9772 -8.7284 224 239 (R)(R)
2384.0091 2383.9524 23.8063 519 549 (R)(G)
2526.2665 2526.3164 -19.7518 433 455 (R)(E)

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m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)
1179.5823	1179.6010	-15.7976	377	386	(K)(H)
1340.6354	1340.6698	-25.6549	365	376	(K)(Y)
1475.7534	1475.7858	-21.9650	200	211	(R)(W)
1475.7534	1475.7494	2.6901	212	223	(K)(T)
1993.9598	1993.9772	-8.7284	224	239	(R)(R)
2384.0091	2383.9524	23.8063	519	549	(R)(G)
2526.2665	2526.3164	-19.7518	433	455	(R)(E)

The matched peptides cover 18% (116/644AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 15.0 Mean Error = -9.3430 ppm Data Tolerance = 34.8276 ppm Mean Missed Cleavages = 0.6

5. 8/40 matches (20%). 91679.6 Da, pI = 9.51. Acc. # O14511. HUMAN. PRO-NEUREGULIN-2 PRECURSOR (PRO-NRG2) [CONTAINS: NEUREGULIN-2 (NRG-2) (NEURAL-AND THYMUS-DERIVED ACTIVATOR FOR ERBB KINASES) (NTAK) (DIVERGENT OF NEUREGULIN 1) (DON-1)].

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
842.4877 842.4848 3.4423 748 755 (R)(A)
1049.4319 1049.4508 -18.0266 547 556 (K)(A)
1475.7534 1475.8130 -40.3781 432 442 (K)(Q)
1784.8783 1784.8861 -4.3812 1 16 (-)MRQVCCSALPPPPLEK(G) 1Met-ox
2195.9922 2196.0296 -17.0487 257 275 (K)CEAAAGNPQPSYRWFKDGK(E) 1Cys-am
2211.0780 2211.1118 -15.3005 135 157 (K)(V)
2384.0091 2384.0725 -26.5725 351 371 (K)SYCVNGGVCYYIEGINQLSCK(C) 1Cys-am
2526.2665 2526.1467 47.4017 351 371 (K)SYCVNGGVCYYIEGINQLSCK(C) 3Cys-am

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Click individual mass to do a non-specific cleavage search.

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
842.4877	842.4848	3.4423	748	755	(R)(A)
1049.4319	1049.4508	-18.0266	547	556	(K)(A)
1475.7534	1475.8130	-40.3781	432	442	(K)(Q)
1784.8783	1784.8861	-4.3812	1	16	(-)MRQVCCSALPPPPLEK(G)	1Met-ox
2195.9922	2196.0296	-17.0487	257	275	(K)CEAAAGNPQPSYRWFKDGK(E)	1Cys-am
2211.0780	2211.1118	-15.3005	135	157	(K)(V)
2384.0091	2384.0725	-26.5725	351	371	(K)SYCVNGGVCYYIEGINQLSCK(C)	1Cys-am
2526.2665	2526.1467	47.4017	351	371	(K)SYCVNGGVCYYIEGINQLSCK(C)	3Cys-am

The matched peptides cover 12% (108/850AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 20.0 Mean Error = -8.8580 ppm Data Tolerance = 52.5709 ppm Mean Missed Cleavages = 0.9