MS-Fit Search Results

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Sample ID (comment): HSG315
Database searched: SwissProt.8.28.2000
Molecular weight search (1000 - 100000 Da) selects 83787 entries.
Full pI range: 88628 entries.
Species search ( HUMAN MOUSE ) selects 9907 entries.
Combined molecular weight, pI and species searches select 8922 entries.
Pre searches select 8922 entries.
MS-Fit search selects 655 entries (results displayed for top 5 matches).

Parameters Used in Search

Considered modifications: | Oxidation of M | Protein N-terminus Acetylated | Acrylamide Modified Cys |

4	50.000 ppm	monoisotopic	Trypsin	4	unmodified	Hydrogen (H)	Free Acid (O H)
Min. # Peptides to Match	Peptide Mass Tolerance (+/-)	Peptide Masses are	Digest Used	Max. # Missed Cleavages	Cysteines Modified by	Peptide N terminus	Peptide C terminus

Result Summary

Rank	MOWSE Score	# (%) Masses Matched	Protein MW (Da)/pI	Species	Protein Name
1	4.49e+003	8/40 (20%)	66018.2 / 8.16	HUMAN	KERATIN, TYPE II CYTOSKELETAL 1 (CYTOKERATIN 1) (K1) (CK 1) (67 KD CYTOKERATIN) (HAIR ALPHA PROTEIN)
2	4.13e+003	9/40 (22%)	70643.4 / 9.48	MOUSE	POLYADENYLATE-BINDING PROTEIN 1 (POLY(A) BINDING PROTEIN 1) (PABP 1)
3	4.13e+003	10/40 (25%)	70671.5 / 9.51	HUMAN	POLYADENYLATE-BINDING PROTEIN 1 (POLY(A) BINDING PROTEIN 1) (PABP 1)
4	290	7/40 (17%)	51295.6 / 4.88	HUMAN	GA BINDING PROTEIN ALPHA CHAIN (GABP-ALPHA SUBUNIT) (TRANSCRIPTION FACTOR E4TF1-60) (NUCLEAR RESPIRATORY FACTOR-2 SUBUNIT ALPHA)
5	243	7/40 (17%)	62630.8 / 9.16	MOUSE	ZINC FINGER PROTEIN 37 (ZFP-37) (MALE GERM CELL SPECIFIC ZINC FINGER PROTEIN)

Detailed Results

1. 8/40 matches (20%). 66018.2 Da, pI = 8.16. Acc. # P04264. HUMAN. KERATIN, TYPE II CYTOSKELETAL 1 (CYTOKERATIN 1) (K1) (CK 1) (67 KD CYTOKERATIN) (HAIR ALPHA PROTEIN).

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)
1179.6365	1179.6010	30.1552	377	386	(K)(H)
1277.7267	1277.7105	12.6285	473	483	(K)(T)
1383.6997	1383.6909	6.4020	186	197	(K)(V)
1475.7776	1475.7858	-5.5351	200	211	(R)(W)
1475.7776	1475.7494	19.1204	212	223	(K)(T)
1487.7522	1487.8043	-35.0023	240	251	(R)(L)
1716.8580	1716.8517	3.7089	418	432	(K)(G)
1994.0117	1993.9772	17.2910	224	239	(R)(R)
2384.0377	2383.9524	35.7746	519	549	(R)(G)

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The matched peptides cover 20% (131/644AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 20.0 Mean Error = 9.3937 ppm Data Tolerance = 42.0094 ppm Mean Missed Cleavages = 0.3

2. 9/40 matches (22%). 70643.4 Da, pI = 9.48. Acc. # P29341. MOUSE. POLYADENYLATE-BINDING PROTEIN 1 (POLY(A) BINDING PROTEIN 1) (PABP 1).

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
1045.5129 1045.5107 2.0599 232 240 (K)(H)
1157.6708 1157.6683 2.1797 31 41 (K)(V)
1542.9310 1542.9372 -4.0139 357 370 (R)(K)
1676.7898 1676.7630 15.9556 334 348 (K)GFGFVCFSSPEEATK(A) 1Cys-am
1740.8254 1740.7982 15.6289 139 153 (K)(A)
1820.8900 1820.8893 0.3936 114 129 (K)ALYDTFSAFGNILSCK(V) 1Cys-am
1820.8900 1820.8529 20.3762 332 348 (R)(A)
1928.9600 1928.9143 23.7146 51 67 (R)(A)
2085.0299 2085.0154 6.9738 50 67 (R)(A)
3264.6547 3264.6725 -5.4715 285 311 (K)(K)

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m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
1045.5129	1045.5107	2.0599	232	240	(K)(H)
1157.6708	1157.6683	2.1797	31	41	(K)(V)
1542.9310	1542.9372	-4.0139	357	370	(R)(K)
1676.7898	1676.7630	15.9556	334	348	(K)GFGFVCFSSPEEATK(A)	1Cys-am
1740.8254	1740.7982	15.6289	139	153	(K)(A)
1820.8900	1820.8893	0.3936	114	129	(K)ALYDTFSAFGNILSCK(V)	1Cys-am
1820.8900	1820.8529	20.3762	332	348	(R)(A)
1928.9600	1928.9143	23.7146	51	67	(R)(A)
2085.0299	2085.0154	6.9738	50	67	(R)(A)
3264.6547	3264.6725	-5.4715	285	311	(K)(K)

The matched peptides cover 19% (127/636AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 22.5 Mean Error = 7.7797 ppm Data Tolerance = 20.8168 ppm Mean Missed Cleavages = 0.6

3. 10/40 matches (25%). 70671.5 Da, pI = 9.51. Acc. # P11940. HUMAN. POLYADENYLATE-BINDING PROTEIN 1 (POLY(A) BINDING PROTEIN 1) (PABP 1).

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
1045.5129 1045.5107 2.0599 232 240 (K)(H)
1157.6708 1157.6683 2.1797 31 41 (K)(V)
1542.9310 1542.9372 -4.0139 357 370 (R)(K)
1676.7898 1676.7630 15.9556 334 348 (K)GFGFVCFSSPEEATK(A) 1Cys-am
1740.8254 1740.7982 15.6289 139 153 (K)(A)
1820.8900 1820.8893 0.3936 114 129 (K)ALYDTFSAFGNILSCK(V) 1Cys-am
1820.8900 1820.8529 20.3762 332 348 (R)(A)
1928.9600 1928.9143 23.7146 51 67 (R)(A)
2085.0299 2085.0154 6.9738 50 67 (R)(A)
2284.2336 2284.2171 7.2510 158 176 (K)MNGMLLNDRKVFVGRFKSR(K) 1Met-ox
3264.6547 3264.6725 -5.4715 285 311 (K)(K)

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m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
1045.5129	1045.5107	2.0599	232	240	(K)(H)
1157.6708	1157.6683	2.1797	31	41	(K)(V)
1542.9310	1542.9372	-4.0139	357	370	(R)(K)
1676.7898	1676.7630	15.9556	334	348	(K)GFGFVCFSSPEEATK(A)	1Cys-am
1740.8254	1740.7982	15.6289	139	153	(K)(A)
1820.8900	1820.8893	0.3936	114	129	(K)ALYDTFSAFGNILSCK(V)	1Cys-am
1820.8900	1820.8529	20.3762	332	348	(R)(A)
1928.9600	1928.9143	23.7146	51	67	(R)(A)
2085.0299	2085.0154	6.9738	50	67	(R)(A)
2284.2336	2284.2171	7.2510	158	176	(K)MNGMLLNDRKVFVGRFKSR(K)	1Met-ox
3264.6547	3264.6725	-5.4715	285	311	(K)(K)

The matched peptides cover 22% (146/636AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 25.0 Mean Error = 7.7316 ppm Data Tolerance = 19.7511 ppm Mean Missed Cleavages = 0.9

4. 7/40 matches (17%). 51295.6 Da, pI = 4.88. Acc. # Q06546. HUMAN. GA BINDING PROTEIN ALPHA CHAIN (GABP-ALPHA SUBUNIT) (TRANSCRIPTION FACTOR E4TF1-60) (NUCLEAR RESPIRATORY FACTOR-2 SUBUNIT ALPHA).

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
1307.6855 1307.6959 -7.9697 405 416 (K)(L)
1383.6997 1383.7457 -33.2222 296 307 (K)(S)
1699.8073 1699.7750 19.0296 221 233 (R)ELCSLNQEDFFQR(V) 1Cys-am
1716.8580 1716.8404 10.2520 151 165 (K)(W)
1820.8900 1820.9805 -49.6976 365 379 (K)(Y)
1928.9600 1928.9275 16.8366 204 220 (K)EFSMTDIDLTTLNISGR(E) 1Met-ox
2510.2389 2510.2019 14.7095 59 79 (R)LQCSLDAHEICLQDIQLDPER(S) 1Cys-am

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m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
1307.6855	1307.6959	-7.9697	405	416	(K)(L)
1383.6997	1383.7457	-33.2222	296	307	(K)(S)
1699.8073	1699.7750	19.0296	221	233	(R)ELCSLNQEDFFQR(V)	1Cys-am
1716.8580	1716.8404	10.2520	151	165	(K)(W)
1820.8900	1820.9805	-49.6976	365	379	(K)(Y)
1928.9600	1928.9275	16.8366	204	220	(K)EFSMTDIDLTTLNISGR(E)	1Met-ox
2510.2389	2510.2019	14.7095	59	79	(R)LQCSLDAHEICLQDIQLDPER(S)	1Cys-am

The matched peptides cover 23% (105/454AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 17.5 Mean Error = -4.2945 ppm Data Tolerance = 54.6203 ppm Mean Missed Cleavages = 0.6

5. 7/40 matches (17%). 62630.8 Da, pI = 9.16. Acc. # P17141. MOUSE. ZINC FINGER PROTEIN 37 (ZFP-37) (MALE GERM CELL SPECIFIC ZINC FINGER PROTEIN).

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
1179.6365 1179.5945 35.6870 365 374 (K)(I)
1709.7725 1709.7263 26.9937 263 277 (R)(A)
1762.8459 1762.7893 32.1112 375 389 (R)IHTGEKPFECNECGK(T) 1Cys-am
1820.8900 1820.8311 32.3388 459 473 (R)VHTGEKPYECVECGK(A) 2Cys-am
1928.9600 1928.9071 27.4355 172 187 (K)CEKVCRHSASHTKEDK(I) 1Cys-am
2384.0377 2384.0651 -11.5036 235 254 (R)THTGEKPYECNECGIAFSQK(S) 2Cys-am
2717.1584 2717.2940 -49.9032 1 25 (-)MGNQDPKQDIVSVLEEEEPSSGKGK(K) 1Met-ox

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m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
1179.6365	1179.5945	35.6870	365	374	(K)(I)
1709.7725	1709.7263	26.9937	263	277	(R)(A)
1762.8459	1762.7893	32.1112	375	389	(R)IHTGEKPFECNECGK(T)	1Cys-am
1820.8900	1820.8311	32.3388	459	473	(R)VHTGEKPYECVECGK(A)	2Cys-am
1928.9600	1928.9071	27.4355	172	187	(K)CEKVCRHSASHTKEDK(I)	1Cys-am
2384.0377	2384.0651	-11.5036	235	254	(R)THTGEKPYECNECGIAFSQK(S)	2Cys-am
2717.1584	2717.2940	-49.9032	1	25	(-)MGNQDPKQDIVSVLEEEEPSSGKGK(K)	1Met-ox

The matched peptides cover 20% (116/553AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 17.5 Mean Error = 13.3085 ppm Data Tolerance = 64.3672 ppm Mean Missed Cleavages = 0.9