MS-Fit Search Results

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Sample ID (comment): HSG317
Database searched: SwissProt.8.28.2000
Molecular weight search (1000 - 100000 Da) selects 83787 entries.
Full pI range: 88628 entries.
Species search ( HUMAN MOUSE ) selects 9907 entries.
Combined molecular weight, pI and species searches select 8922 entries.
Pre searches select 8922 entries.
MS-Fit search selects 479 entries (results displayed for top 5 matches).

Parameters Used in Search

Considered modifications: | Oxidation of M | Protein N-terminus Acetylated | Acrylamide Modified Cys |

4	50.000 ppm	monoisotopic	Trypsin	4	unmodified	Hydrogen (H)	Free Acid (O H)
Min. # Peptides to Match	Peptide Mass Tolerance (+/-)	Peptide Masses are	Digest Used	Max. # Missed Cleavages	Cysteines Modified by	Peptide N terminus	Peptide C terminus

Result Summary

Rank	MOWSE Score	# (%) Masses Matched	Protein MW (Da)/pI	Species	Protein Name
1	7.71e+006	16/34 (47%)	49542.0 / 7.26	HUMAN	ELONGATION FACTOR TU, MITOCHONDRIAL PRECURSOR (P43)
2	141	5/34 (14%)	41523.1 / 6.08	MOUSE	GUANINE NUCLEOTIDE-BINDING PROTEIN, ALPHA-14 SUBUNIT
3	135	4/34 (11%)	36297.6 / 7.08	HUMAN	L-LACTATE DEHYDROGENASE X CHAIN (LDH-C)
4	66.4	4/34 (11%)	54872.2 / 8.12	HUMAN	HYPOTHETICAL PROTEIN DJ845O24.3
5	60.1	5/34 (14%)	19209.5 / 9.51	HUMAN	ZINC FINGER PROTEIN 92 (ZINC FINGER PROTEIN HTF12)

Detailed Results

1. 16/34 matches (47%). 49542.0 Da, pI = 7.26. Acc. # P49411. HUMAN. ELONGATION FACTOR TU, MITOCHONDRIAL PRECURSOR (P43).

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
775.4958	775.4830	16.5032	406	411	(K)(Q)
851.5385	851.5103	33.0960	163	169	(R)(Q)
1032.5802	1032.5689	10.8879	272	281	(R)(G)
1149.6434	1149.6519	-7.4621	352	361	(K)(E)
1185.6537	1185.6228	26.1179	316	327	(R)(G)
1364.6295	1364.5970	23.7896	92	102	(K)(A)
1483.8549	1483.8273	18.6272	170	182	(R)(A)
1483.8549	1483.8233	21.3383	316	330	(R)(R)
1492.7500	1492.6920	38.8588	91	102	(K)(A)
1542.8843	1542.8532	20.2036	239	252	(K)(D)
1673.8986	1673.8611	22.3825	105	120	(R)(H)
1872.0017	1871.9537	25.6686	210	227	(K)GEETPVIVGSALCALEGR(D)	1Cys-am
1875.0079	1875.0050	1.5521	396	411	(K)ELAMPGEDLKFNLILR(Q)	1Met-ox
2046.0534	2046.0065	22.9134	183	200	(K)(E)
2062.0451	2062.0014	21.1976	183	200	(K)ADAVQDSEMVELVELEIR(E)	1Met-ox
2129.2278	2129.1647	29.6668	253	271	(R)(G)
2624.4213	2624.3605	23.1538	210	234	(K)GEETPVIVGSALCALEGRDPELGLK(S)	1Cys-am

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The matched peptides cover 38% (175/452AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 47.1 Mean Error = 20.4997 ppm Data Tolerance = 21.9049 ppm Mean Missed Cleavages = 0.2

2. 5/34 matches (14%). 41523.1 Da, pI = 6.08. Acc. # P30677. MOUSE. GUANINE NUCLEOTIDE-BINDING PROTEIN, ALPHA-14 SUBUNIT.

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
1542.8843 1542.8128 46.3954 104 116 (K)(V)
1940.9452 1940.9176 14.2065 319 334 (K)VIYSHFTCATDTENIR(F) 1Cys-am
2046.0534 2046.0047 23.8179 279 295 (K)(Q)
2062.0451 2061.9996 22.0950 279 295 (K)IMYSHLISYFPEYTGPK(Q) 1Met-ox
2079.0959 2079.1636 -32.5755 38 56 (K)(I)

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m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
1542.8843	1542.8128	46.3954	104	116	(K)(V)
1940.9452	1940.9176	14.2065	319	334	(K)VIYSHFTCATDTENIR(F)	1Cys-am
2046.0534	2046.0047	23.8179	279	295	(K)(Q)
2062.0451	2061.9996	22.0950	279	295	(K)IMYSHLISYFPEYTGPK(Q)	1Met-ox
2079.0959	2079.1636	-32.5755	38	56	(K)(I)

The matched peptides cover 18% (65/355AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 14.7 Mean Error = 14.7879 ppm Data Tolerance = 58.1171 ppm Mean Missed Cleavages = 0.6

3. 4/34 matches (11%). 36297.6 Da, pI = 7.08. Acc. # P07864. HUMAN. L-LACTATE DEHYDROGENASE X CHAIN (LDH-C).

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
842.5435 842.5100 39.7597 150 157 (K)(V)
1364.6295 1364.6004 21.3194 12 22 (K)LIEDDENSQCK(I) 1Cys-am
1475.8225 1475.7932 19.8530 285 297 (K)(N)
2002.1436 2002.1445 -0.4221 23 42 (K)ITIVGTGAVGMACAISILLK(D) 1Cys-am

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m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
842.5435	842.5100	39.7597	150	157	(K)(V)
1364.6295	1364.6004	21.3194	12	22	(K)LIEDDENSQCK(I)	1Cys-am
1475.8225	1475.7932	19.8530	285	297	(K)(N)
2002.1436	2002.1445	-0.4221	23	42	(K)ITIVGTGAVGMACAISILLK(D)	1Cys-am

The matched peptides cover 15% (52/332AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 11.8 Mean Error = 20.1275 ppm Data Tolerance = 32.8472 ppm Mean Missed Cleavages = 0.0

4. 4/34 matches (11%). 54872.2 Da, pI = 8.12. Acc. # O60811. HUMAN. HYPOTHETICAL PROTEIN DJ845O24.3.

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
1466.8334 1466.8405 -4.8606 80 92 (K)(D)
1673.8986 1673.9379 -23.4946 276 289 (K)(C)
1851.9211 1851.8562 35.0502 116 131 (R)WPGAWALSCFPEAMSK(R) 1Cys-am
2624.4213 2624.3481 27.8782 425 445 (R)(Q)

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m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
1466.8334	1466.8405	-4.8606	80	92	(K)(D)
1673.8986	1673.9379	-23.4946	276	289	(K)(C)
1851.9211	1851.8562	35.0502	116	131	(R)WPGAWALSCFPEAMSK(R)	1Cys-am
2624.4213	2624.3481	27.8782	425	445	(R)(Q)

The matched peptides cover 13% (64/474AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 11.8 Mean Error = 8.6433 ppm Data Tolerance = 55.1665 ppm Mean Missed Cleavages = 0.5

5. 5/34 matches (14%). 19209.5 Da, pI = 9.51. Acc. # Q03936. HUMAN. ZINC FINGER PROTEIN 92 (ZINC FINGER PROTEIN HTF12).

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
1179.6248 1179.6196 4.4024 1 10 (-)MGPLTFRDVK(I) 1Met-ox
1872.0017 1871.9624 21.0044 149 163 (K)SFCMLSQLTQHKKIH(-) 1Cys-am
2062.0451 2061.9915 25.9969 93 111 (K)(I)
2384.0362 2384.1233 -36.5107 11 30 (K)(D)
2624.4213 2624.3924 11.0175 112 132 (K)(I)

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m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
1179.6248	1179.6196	4.4024	1	10	(-)MGPLTFRDVK(I)	1Met-ox
1872.0017	1871.9624	21.0044	149	163	(K)SFCMLSQLTQHKKIH(-)	1Cys-am
2062.0451	2061.9915	25.9969	93	111	(K)(I)
2384.0362	2384.1233	-36.5107	11	30	(K)(D)
2624.4213	2624.3924	11.0175	112	132	(K)(I)

The matched peptides cover 52% (85/163AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 14.7 Mean Error = 5.1821 ppm Data Tolerance = 49.5636 ppm Mean Missed Cleavages = 1.6