MS-Fit Search Results


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Sample ID (comment): HSG318
Database searched: NCBInr.1.2.2001
Molecular weight search (1000 - 100000 Da) selects 571223 entries.
Full pI range: 599452 entries.
Species search ( HUMAN MOUSE ) selects 56190 entries.
Combined molecular weight, pI and species searches select 52818 entries.
Pre searches select 52818 entries.
MS-Fit search selects 1266 entries (results displayed for top 5 matches).

Parameters Used in Search

Considered modifications: | Oxidation of M | Protein N-terminus Acetylated | Acrylamide Modified Cys |
Min. # Peptides
to Match
Peptide Mass
Tolerance (+/-)
Peptide Masses
are
Digest
Used
Max. # Missed
Cleavages
Cysteines
Modified by
Peptide
N terminus
Peptide
C terminus
4 75.000 ppm monoisotopic Trypsin 3 carbamidomethylation Hydrogen (H) Free Acid (O H)

Result Summary

RankMOWSE
Score
# (%)
Masses
Matched
Protein
MW (Da)/pI
SpeciesAccession #Protein Name
11.78e+0038/32 (25%) 23635.3 / 9.57HOMO SAPIENS(U72511) B-cell receptor associated protein
21928/32 (25%) 81463.7 / 8.31HOMO SAPIENS(AF223470) KIAA0971-I protein
31284/32 (12%) 22926.7 / 6.97HOMO SAPIENS(AF191795) immunoglobulin lambda light chain
472.24/32 (12%) 24859.9 / 8.21HUMANIg lambda chain
569.37/32 (21%) 80497.3 / 6.18HOMO SAPIENS(AF151685) dynamin-like protein DYNIV-11

Detailed Results

1. 8/32 matches (25%). 23635.3 Da, pI = 9.57. Acc. # 1673514. HOMO SAPIENS. (U72511) B-cell receptor associated protein .
m/z
submitted
MH+
matched
Delta
ppm
startendPeptide Sequence
(Click for Fragment Ions)
Modifications
994.5268994.484642.4429 36 43(R)(V)
1177.69021177.632948.6317 60 69(K)(A)
1204.70081204.636053.8426 10 19(K)DLQMVNISLR(V)1Met-ox
1889.02271888.970327.7243 20 35(R)(L)
1905.04281904.965340.7198 20 35(R)VLSRPNAQELPSMYQR(L)1Met-ox
2225.24242225.145443.5887 183 201(R)(G)
2240.27612240.181442.2462 84 103(R)(E)
2301.34272301.212456.6208 135 156(R)(N)

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Click individual mass to do a non-specific cleavage search.

The matched peptides cover 49% (105/211AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 25.0 Mean Error = 44.4771 ppm Data Tolerance = 17.8425 ppm Mean Missed Cleavages = 0.4

2. 8/32 matches (25%). 81463.7 Da, pI = 8.31. Acc. # 7578791. HOMO SAPIENS. (AF223470) KIAA0971-I protein .
m/z
submitted
MH+
matched
Delta
ppm
startendPeptide Sequence
(Click for Fragment Ions)
Modifications
994.5268994.52095.8554 549 556(K)(D)
1177.69021177.613864.8483 359 368(R)(V)
1723.99591723.930438.0004 1 16(-)(M)
1889.02271889.002010.9545 693 710(K)(-)
1905.04281904.940853.5817 595 609(K)(M)
2211.20252211.151722.9684 1 20(-)(A)
2247.23282247.080367.8806 340 358(R)FSVLNSQHMFEVLAAMNHR(S)1Met-ox
2284.24712284.100764.0835 661 679(R)HLNAMGFHVILVNNWEMDK(L)1Met-ox

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Click individual mass to do a non-specific cleavage search.

The matched peptides cover 15% (109/710AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 25.0 Mean Error = 41.0216 ppm Data Tolerance = 50.4520 ppm Mean Missed Cleavages = 0.1

3. 4/32 matches (12%). 22926.7 Da, pI = 6.97. Acc. # 6467839. HOMO SAPIENS. (AF191795) immunoglobulin lambda light chain .
m/z
submitted
MH+
matched
Delta
ppm
startendPeptide Sequence
(Click for Fragment Ions)
Modifications
1723.99591723.897956.8803 1 17(-)(V)
1767.91701767.9605-24.5862 63 80(R)(S)
2211.20252211.152422.6735 134 153(K)(A)
2225.24242225.168033.4094 134 153(K)ATLVCLISDFYPGAVTVAWK(A)1Cys-am

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Click individual mass to do a non-specific cleavage search.

The matched peptides cover 25% (55/216AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 12.5 Mean Error = 22.0943 ppm Data Tolerance = 68.4836 ppm Mean Missed Cleavages = 0.3

4. 4/32 matches (12%). 24859.9 Da, pI = 8.21. Acc. # 106645. HUMAN. Ig lambda chain.
m/z
submitted
MH+
matched
Delta
ppm
startendPeptide Sequence
(Click for Fragment Ions)
Modifications
1859.96831860.0166-25.9248 67 82(K)(F)
1876.01701876.01152.9317 67 82(K)LMIYEVTKRPSGVPNR(F)1Met-ox
2211.20252211.152422.6735 153 172(K)(A)
2225.24242225.168033.4094 153 172(K)ATLVCLISDFYPGAVTVAWK(A)1Cys-am

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Click individual mass to do a non-specific cleavage search.

The matched peptides cover 15% (36/235AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 12.5 Mean Error = 8.2725 ppm Data Tolerance = 52.1183 ppm Mean Missed Cleavages = 0.5

5. 7/32 matches (21%). 80497.3 Da, pI = 6.18. Acc. # 5081794. HOMO SAPIENS. (AF151685) dynamin-like protein DYNIV-11 .
m/z
submitted
MH+
matched
Delta
ppm
startendPeptide Sequence
(Click for Fragment Ions)
Modifications
1723.99591723.924441.5136 273 287(K)(T)
1853.01941853.0609-22.3874 239 255(K)(K)
1876.01701875.938741.7236 442 456(R)IIQHCSNYSTQELLR(F)1Cys-am
1889.02271888.966329.8539 624 638(R)(L)
2211.20252211.2236-9.5495 377 397(R)(N)
2223.24682223.092769.3371 682 700(K)SSLLDDLLTESEDMAQRRK(E)1Met-ox
2284.24712284.2705-10.2670 398 418(R)(R)
2284.24712284.106661.5120 424 441(R)LEEPSLRCVELVHEEMQR(I)1Met-ox 1Cys-am

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Click individual mass to do a non-specific cleavage search.

The matched peptides cover 19% (141/725AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 21.9 Mean Error = 25.2170 ppm Data Tolerance = 69.9284 ppm Mean Missed Cleavages = 1.1


MS-Fit 3.3.1, ProteinProspector 3.4.1
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