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NiceProt View of Swiss-Prot: P02248

[General] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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General information about the entry
Entry name UBIQ_HUMAN
Primary accession number P02248
Secondary accession numbers P02249 P02250 Q29120 Q91887 Q91888
Entered in Swiss-Prot in Release 01, July 1986
Sequence was last modified in Release 05, August 1987
Annotations were last modified in    Release 41, February 2003
Name and origin of the protein
Protein name Ubiquitin
Synonyms None
Gene names
 UBA52 or UBCEP2
and UBB
and UBC
and UBA80 or UBCEP1 or RPS27A
From
Homo sapiens (Human) [TaxID: 9606]
Mus musculus (Mouse) [TaxID: 10090]
Rattus norvegicus (Rat) [TaxID: 10116]
Bos taurus (Bovine) [TaxID: 9913]
Cavia porcellus (Guinea pig) [TaxID: 10141]
Cricetulus griseus (Chinese hamster) [TaxID: 10029]
Cricetulus longicaudatus (Long-tailed hamster) (Chinese hamster) [TaxID: 10030]
Oryctolagus cuniculus (Rabbit) [TaxID: 9986]
Sus scrofa (Pig) [TaxID: 9823]
Gallus gallus (Chicken) [TaxID: 9031]
Xenopus laevis (African clawed frog) [TaxID: 8355]
Taxonomy Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo.
References
[1]
SEQUENCE FROM NUCLEIC ACID.
SPECIES=Human;
MEDLINE=85230546; PubMed=2988935; [NCBI, ExPASy, EBI, Israel, Japan]
Wiborg O., Pedersen M.S., Wind A., Berglund L.E., Marcker K.A., Vuust J.;
"The human ubiquitin multigene family: some genes contain multiple directly repeated ubiquitin coding sequences.";
EMBO J. 4:755-759(1985).
[2]
SEQUENCE FROM NUCLEIC ACID.
SPECIES=Human;
MEDLINE=87326412; PubMed=2820408; [NCBI, ExPASy, EBI, Israel, Japan]
Einspanier R., Sharma H.S., Scheit K.H.;
"Cloning and sequence analysis of a cDNA encoding poly-ubiquitin in human ovarian granulosa cells.";
Biochem. Biophys. Res. Commun. 147:581-587(1987).
[3]
SEQUENCE FROM NUCLEIC ACID (UBB).
SPECIES=Human;
TISSUE=Lymphocytes;
MEDLINE=87146371; PubMed=3029682; [NCBI, ExPASy, EBI, Israel, Japan]
Baker R.T., Board P.G.;
"The human ubiquitin gene family: structure of a gene and pseudogenes from the Ub B subfamily.";
Nucleic Acids Res. 15:443-463(1987).
[4]
SEQUENCE FROM NUCLEIC ACID (UBA52).
SPECIES=Human;
MEDLINE=91212181; PubMed=1850507; [NCBI, ExPASy, EBI, Israel, Japan]
Baker R.T., Board P.G.;
"The human ubiquitin-52 amino acid fusion protein gene shares several structural features with mammalian ribosomal protein genes.";
Nucleic Acids Res. 19:1035-1040(1991).
[5]
SEQUENCE OF 5-76 FROM NUCLEIC ACID (UBA80).
SPECIES=Human;
MEDLINE=85207809; PubMed=2581967; [NCBI, ExPASy, EBI, Israel, Japan]
Lund P.K., Moats-Staats B.M., Simmons J.G., Hoyt E., D'Ercole A.J., Martin F., van Wyk J.J.;
"Nucleotide sequence analysis of a cDNA encoding human ubiquitin reveals that ubiquitin is synthesized as a precursor.";
J. Biol. Chem. 260:7609-7613(1985).
[6]
SEQUENCE OF 1-74.
SPECIES=Human;
MEDLINE=75156547; PubMed=124018; [NCBI, ExPASy, EBI, Israel, Japan]
Schlesinger D.H., Goldstein G.;
"Hybrid troponin reconstituted from vertebrate and arthropod subunits.";
Nature 255:423-424(1975).
[7]
SEQUENCE FROM NUCLEIC ACID.
SPECIES=Mouse;
STRAIN=C57BL/6;
MEDLINE=90245601; PubMed=2159627; [NCBI, ExPASy, EBI, Israel, Japan]
Finch J.S., Bonham K., Krieg P., Bowden G.T.;
"Murine polyubiquitin mRNA sequence.";
Nucleic Acids Res. 18:1907-1907(1990).
[8]
SEQUENCE.
SPECIES=Rat;
TISSUE=Duodenum;
STRAIN=Wistar;
MEDLINE=94304928; PubMed=8031840; [NCBI, ExPASy, EBI, Israel, Japan]
Hubbard M.J., Carne A.;
"Differential feeding-related regulation of ubiquitin and calbindin9kDa in rat duodenum.";
Biochim. Biophys. Acta 1200:191-196(1994).
[9]
SEQUENCE FROM NUCLEIC ACID (UBA52).
SPECIES=Rat;
STRAIN=Sprague-Dawley;
MEDLINE=96011832; PubMed=7488009; [NCBI, ExPASy, EBI, Israel, Japan]
Chan Y.-L., Suzuki K., Wool I.G.;
"The carboxyl extensions of two rat ubiquitin fusion proteins are ribosomal proteins S27a and L40.";
Biochem. Biophys. Res. Commun. 215:682-690(1995).
[10]
SEQUENCE FROM NUCLEIC ACID.
SPECIES=Bovine;
TISSUE=Seminal vesicle;
MEDLINE=93176814; PubMed=8382528; [NCBI, ExPASy, EBI, Israel, Japan]
Wempe F., Scheit K.H.;
"Characterization of a full-length cDNA encoding a bovine four tandem-repeat ubiquitin.";
Biochim. Biophys. Acta 1172:209-211(1993).
[11]
SEQUENCE OF 1-74.
SPECIES=Bovine;
MEDLINE=75205496; PubMed=1170880; [NCBI, ExPASy, EBI, Israel, Japan]
Schlesinger D.H., Goldstein G., Niall H.D.;
"The complete amino acid sequence of ubiquitin, an adenylate cyclase stimulating polypeptide probably universal in living cells.";
Biochemistry 14:2214-2218(1975).
[12]
SEQUENCE OF 1-50.
SPECIES=Bovine;
MEDLINE=81062406; PubMed=6254502; [NCBI, ExPASy, EBI, Israel, Japan]
Hamilton J.W., Rouse J.B.;
"The biosynthesis of ubiquitin by parathyroid gland.";
Biochem. Biophys. Res. Commun. 96:114-120(1980).
[13]
SEQUENCE FROM NUCLEIC ACID.
SPECIES=Pig;
MEDLINE=88054463; PubMed=2824145; [NCBI, ExPASy, EBI, Israel, Japan]
Einspanier R., Sharma H.S., Scheit K.H.;
"An mRNA encoding poly-ubiquitin in porcine corpus luteum: identification by cDNA cloning and sequencing.";
DNA 6:395-400(1987).
[14]
SEQUENCE FROM NUCLEIC ACID.
SPECIES=C.porcellus;
TISSUE=Spleen;
Tsukagoshi N.;
"Ascorbate-dependent expression of ubiquitin genes in guinea pig.";
Submitted (FEB-1996) to the EMBL/GenBank/DDBJ databases.
[15]
SEQUENCE FROM NUCLEIC ACID.
SPECIES=C.griseus;
TISSUE=Lung fibroblast;
STRAIN=V79;
MEDLINE=92223105; PubMed=1314094; [NCBI, ExPASy, EBI, Israel, Japan]
Nenoi M., Mita K., Ichimura S.;
"Evolutionarily conserved structure of the 3' non-translated region of a Chinese hamster polyubiquitin gene.";
Biochim. Biophys. Acta 1130:247-252(1992).
[16]
SEQUENCE FROM NUCLEIC ACID.
SPECIES=C.longicaudatus;
TISSUE=Lung fibroblast;
STRAIN=V79;
MEDLINE=89160248; PubMed=2537950; [NCBI, ExPASy, EBI, Israel, Japan]
Fornace A.J. Jr., Alamo I. Jr., Hollander M.C., Lamoreaux E.;
"Ubiquitin mRNA is a major stress-induced transcript in mammalian cells.";
Nucleic Acids Res. 17:1215-1230(1989).
[17]
SEQUENCE.
SPECIES=Rabbit;
TISSUE=Brain;
MEDLINE=93149997; PubMed=1337207; [NCBI, ExPASy, EBI, Israel, Japan]
Wajih N., Siddiqi A.R., Kaiser R., Persson B., Zaidi Z.H., Joernvall H.;
"Structural characterization of rabbit brain ubiquitin.";
Protein Seq. Data Anal. 5:31-32(1992).
[18]
SEQUENCE FROM NUCLEIC ACID.
SPECIES=Chicken;
MEDLINE=85213498; PubMed=2987683; [NCBI, ExPASy, EBI, Israel, Japan]
Bond U., Schlesinger M.J.;
"Ubiquitin is a heat shock protein in chicken embryo fibroblasts.";
Mol. Cell. Biol. 5:949-956(1985).
[19]
SEQUENCE FROM NUCLEIC ACID.
SPECIES=Chicken;
MEDLINE=87089821; PubMed=3025663; [NCBI, ExPASy, EBI, Israel, Japan]
Bond U., Schlesinger M.J.;
"The chicken ubiquitin gene contains a heat shock promoter and expresses an unstable mRNA in heat-shocked cells.";
Mol. Cell. Biol. 6:4602-4610(1986).
[20]
SEQUENCE FROM NUCLEIC ACID.
SPECIES=Chicken;
TISSUE=Spermatid;
MEDLINE=89098351; PubMed=2850543; [NCBI, ExPASy, EBI, Israel, Japan]
Mezquita J., Pau M., Mezquita C.;
"cDNA encoding a chicken ubiquitin-fusion protein identical to the corresponding human protein.";
Nucleic Acids Res. 16:11838-11838(1988).
[21]
SEQUENCE FROM NUCLEIC ACID.
SPECIES=X.laevis;
MEDLINE=85048942; PubMed=6209017; [NCBI, ExPASy, EBI, Israel, Japan]
Dworkin-Rastl E., Shrutkowski A., Dworkin M.B.;
"Multiple ubiquitin mRNAs during Xenopus laevis development contain tandem repeats of the 76 amino acid coding sequence.";
Cell 39:321-325(1984).
[22]
X-RAY CRYSTALLOGRAPHY (1.8 ANGSTROMS).
SPECIES=Human;
MEDLINE=87311725; PubMed=3041007; [NCBI, ExPASy, EBI, Israel, Japan]
Vijay-Kumar S., Bugg C.E., Cook W.J.;
"Structure of ubiquitin refined at 1.8-A resolution.";
J. Mol. Biol. 194:531-544(1987).
[23]
X-RAY CRYSTALLOGRAPHY (1.8 ANGSTROMS).
SPECIES=Human;
MEDLINE=94220022; PubMed=8166633; [NCBI, ExPASy, EBI, Israel, Japan]
Ramage R., Green J., Muir T.W., Ogunjobi O.M., Love S., Shaw K.;
"Synthetic, structural and biological studies of the ubiquitin system: the total chemical synthesis of ubiquitin.";
Biochem. J. 299:151-158(1994).
[24]
X-RAY CRYSTALLOGRAPHY (1.8 ANGSTROMS).
SPECIES=Human;
MEDLINE=94149762; PubMed=8107144; [NCBI, ExPASy, EBI, Israel, Japan]
Cook W.J., Jeffrey L.C., Kasperek E., Pickart C.M.;
"Structure of tetraubiquitin shows how multiubiquitin chains can be formed.";
J. Mol. Biol. 236:601-609(1994).
[25]
X-RAY CRYSTALLOGRAPHY (2.3 ANGSTROMS).
SPECIES=Bovine;
MEDLINE=92355616; PubMed=1322903; [NCBI, ExPASy, EBI, Israel, Japan]
Cook W.J., Jeffrey L.C., Carson M., Chen Z., Pickart C.M.;
"Structure of a diubiquitin conjugate and a model for interaction with ubiquitin conjugating enzyme (E2).";
J. Biol. Chem. 267:16467-16471(1992).
Comments
  • FUNCTION: INVOLVED IN THE ATP-DEPENDENT SELECTIVE DEGRADATION OF CELLULAR PROTEINS, THE MAINTENANCE OF CHROMATIN STRUCTURE, THE REGULATION OF GENE EXPRESSION, THE STRESS RESPONSE, AND RIBOSOME BIOGENESIS.
  • SUBCELLULAR LOCATION: Nuclear and cytoplasmic.
  • MISCELLANEOUS: UBIQUITIN IS SYNTHESIZED AS A POLYUBIQUITIN PRECURSOR WITH EXACT HEAD TO TAIL REPEATS, THE NUMBER OF REPEAT DIFFER BETWEEN SPECIES (UP TO 12 IN XENOPUS). IN SOME SPECIES THERE IS A FINAL AMINO-ACID AFTER THE LAST REPEAT, IN HUMAN: VAL, IN CHICKEN: TYR. SOME UBIQUITIN GENES CONTAIN A SINGLE COPY OF UBIQUITIN FUSED TO A RIBOSOMAL PROTEIN (EITHER L40 OR S27A).
Copyright
This SWISS-PROT entry is copyright. It is produced through a collaboration between the Swiss Institute of Bioinformatics and the EMBL outstation - the European Bioinformatics Institute. There are no restrictions on its use by non-profit institutions as long as its content is in no way modified and this statement is not removed. Usage by and for commercial entities requires a license agreement (See http://www.isb-sib.ch/announce/ or send an email to license@isb-sib.ch).
Cross-references
EMBL
M26880; AAA36789.1; ALT_TERM.[EMBL / GenBank / DDBJ] [CoDingSequence]
X04803; CAA28495.1; ALT_TERM.[EMBL / GenBank / DDBJ] [CoDingSequence]
X56997; CAA40312.1; ALT_TERM.[EMBL / GenBank / DDBJ] [CoDingSequence]
X56998; CAA40313.1; ALT_TERM.[EMBL / GenBank / DDBJ] [CoDingSequence]
X56999; CAA40314.1; ALT_TERM.[EMBL / GenBank / DDBJ] [CoDingSequence]
M10939; AAA36788.1; ALT_TERM.[EMBL / GenBank / DDBJ] [CoDingSequence]
X51703; CAA35999.1; ALT_TERM.[EMBL / GenBank / DDBJ] [CoDingSequence]
X82636; CAA57958.1; ALT_TERM.[EMBL / GenBank / DDBJ] [CoDingSequence]
Z18245; CAA79146.1; ALT_TERM.[EMBL / GenBank / DDBJ] [CoDingSequence]
M18159; AAA31133.1; ALT_SEQ.[EMBL / GenBank / DDBJ] [CoDingSequence]
D83208; BAA11842.1; ALT_TERM.[EMBL / GenBank / DDBJ] [CoDingSequence]
D83209; BAA11843.1; ALT_TERM.[EMBL / GenBank / DDBJ] [CoDingSequence]
X60390; CAA42941.1; ALT_TERM.[EMBL / GenBank / DDBJ] [CoDingSequence]
X08013; CAA30815.1; ALT_TERM.[EMBL / GenBank / DDBJ] [CoDingSequence]
X02650; CAA26488.1; -.[EMBL / GenBank / DDBJ] [CoDingSequence]
M14693; AAA49128.1; ALT_TERM.[EMBL / GenBank / DDBJ] [CoDingSequence]
X13493; CAA31848.1; ALT_SEQ.[EMBL / GenBank / DDBJ] [CoDingSequence]
M11512; AAA49978.1; ALT_INIT.[EMBL / GenBank / DDBJ] [CoDingSequence]
PIR A02574; UQHU.
A90388; UQBO.
A02577; UQBOP.
S02825; UQHY.
A22632; UQHUR7.
A22005; A22005.
A26437; A26437.
A29526; A29526.
A29584; A29584.
S12583; S12583.
S28203; S28203.
PDB
1UBQ; 16-JUL-87.[ExPASy / RCSB]
1UBI; 31-MAY-94.[ExPASy / RCSB]
1AAR; 31-OCT-93.[ExPASy / RCSB]
1TBE; 30-APR-94.[ExPASy / RCSB]
Detailed list of linked structures.
SWISS-2DPAGE P02248; HUMAN.
Aarhus/Ghent-2DPAGE 13; IEF.
HSC-2DPAGE P02248; HUMAN.
Siena-2DPAGE P02248; -.
Genew HGNC:12463; UBB.
CleanEx HGNC:12463; UBB.
MIM 191321 [NCBI / EBI].
191339 [NCBI / EBI].
191340 [NCBI / EBI].
191343 [NCBI / EBI].
191320 [NCBI / EBI].
191344 [NCBI / EBI].
GeneCards UBA52.
GeneLynx UBA52; Homo sapiens.
MGD MGI:98888; Ubb.
GeneLynx UBA52; Mus musculus.
SOURCE UBA52; Homo sapiens.
UBA52; Mus musculus.
Ensembl P02248; Homo sapiens. [Entry / Contig view]
P02248; Mus musculus. [Entry / Contig view]
InterPro IPR000626; Ubiquitin.
Graphical view of domain structure.
Pfam PF00240; ubiquitin; 1.
PRINTS PR00348; UBIQUITIN.
SMART SM00213; UBQ; 1.
PROSITE PS00299; UBIQUITIN_1; 1.
PS50053; UBIQUITIN_2; 1.
ProDom [Domain structure / List of seq. sharing at least 1 domain].
BLOCKS P02248.
ProtoNet P02248.
ProtoMap P02248.
PRESAGE P02248.
DIP P02248.
ModBase P02248.
Keywords
Nuclear protein; Polyprotein; 3D-structure.
Features
KeyFrom  To Length Description
SITE   48   48  1     NECESSARY FOR BRANCHED-CHAIN MULTIUBIQUITIN ADDUCTS.
BINDING   54   54        CONJUGATION TO ACTIVATING ENZYME.
SITE   68   68  1     ESSENTIAL FOR FUNCTION.
BINDING   72   72        CONJUGATION TO ACTIVATING ENZYME.
BINDING   76   76        CONJUGATION TO ACCEPTOR PROTEINS.
STRAND   2    7  6     
TURN   8    9  2     
STRAND   12   16  5     
TURN   19   20  2     
STRAND   22   22  1     
HELIX   23   34  12     
HELIX   38   40  3     
STRAND   41   45  5     
TURN   46   47  2     
STRAND   48   49  2     
TURN   52   53  2     
STRAND   55   55  1     
HELIX   56   59  4     
TURN   60   60  1     
TURN   63   64  2     
STRAND   66   71  6     
SEVIEWER logo Feature table viewer
Sequence information
Length: 76 AA Molecular weight: 8565 Da CRC64: C42A35397FFD9B52 [This is a checksum on the sequence]
        10         20         30         40         50         60 
         |          |          |          |          |          | 
MQIFVKTLTG KTITLEVEPS DTIENVKAKI QDKEGIPPDQ QRLIFAGKQL EDGRTLSDYN 

        70 
         | 
IQKESTLHLV LRLRGG 

P02248 in FASTA format

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View entry in raw text format (no links)
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