MS-Fit Search Results

MS-Fit Search Results

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Parameters

Database searched: SwissProt.10.25.2002
Digest Used: Trypsin
Max. # Missed Cleavages: 1
Peptide N terminus: Hydrogen
Peptide C terminus: Free Acid
Cysteine Modification: acrylamide
Instrument Name: MALDI-TOF
Sample ID (comment): 087
Minimum Matches: 4
Sort Type: Score Sort
Considered modifications: | Peptide N-terminal Gln to pyroGlu | Oxidation of M | Protein N-terminus Acetylated |
Min Parent Ion Matches: 1
MOWSE On: 1
MOWSE P Factor: 0.4

Pre Search Results

Number of entries in the database: 116819
Molecular weight search (1000 - 100000 Da) selects 110413 entries.
Full pI range: 116819 entries.
Species search ( HUMAN MOUSE ) selects 14257 entries.
Combined molecular weight, pI and species searches select 12612 entries.
Pre searches select 12612 entries.

Data Set 1 Results

MS-Fit search selects 228 entries (results displayed for top 5 matches).

Results Summary

	MOWSE Score	#/61(%) Masses Matched	% Cov	% TIC	Mean Err ppm	Data Tol ppm	MS-Digest Index #	Protein MW (Da)/pI	Accession #	Species	Protein Name
1	1.190e+004	10 (16)	30.0	16.4	-61.6	57.0		36689/8.4		HUMAN	L-lactate dehydrogenase A chain (LDH-A) (LDH muscle subunit) (LDH-M)
2	1357	5 (8)	9.0	8.2	-12.5	117		84198/5.8		MOUSE	Meprin A alpha-subunit precursor (Endopeptidase-2) (MEP-1)
3	1150	4 (6)	16.0	6.6	19.0	73.0		49478/5.8		HUMAN	Alpha enolase, lung specific (2-phospho-D-glycerate hydro-lyase) (Non-neural enolase) (NNE) (Phosphopyruvate hydratase) (HLE1)
4	1080	7 (11)	13.0	11.5	12.3	81.9		78505/9.2		HUMAN	Sp110 nuclear body protein (Speckled 110 kDa) (Transcriptional coactivator Sp110) (Interferon-induced protein 41/75)
5	731	4 (6)	8.0	6.6	10.2	134		86612/6.8		MOUSE	Putative pre-mRNA splicing factor RNA helicase (DEAH box protein 15)

92337	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	x	.	.	x	x	.	.	.	.	.	x	.	.	.	.	.	x	x	x	.	x	.	x	.	x	.	.	.	.	.	.	.	.	.	.	.
77029	x	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	x	.	.	.	.	x	.	.	.	.	x	.	.	x	.	.	.	.	.	.
26128	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	x	.	.	.	x	.	.	.	.	.	.	.	x	.	.	x	.	.	.
44148	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	x	.	.	x	.	.	.	.	x	x	x	.	.	.	.	.	x	x	.	.	.	.	.	.	.
104682	.	.	.	.	.	.	.	.	.	.	.	.	.	x	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	x	.	x	.	.	.	x	.

Detailed Results

1. 10/61 matches (16%).
Acc. #: Species: HUMAN Name: L-lactate dehydrogenase A chain (LDH-A) (LDH muscle subunit) (LDH-M)
Index: MW: 36689 Da pI: 8.4

m/z Submitted	MH⁺ Matched	Delta ppm	Modifications	Start	End	Missed Cleavages	Database Sequence
1087.4312	1087.5359	-96	1Met-ox	170	177	1	(R)FRYLMGER(L)
1118.4885	1118.5846	-86		319	328	0	(K) (E)
1134.4570	1134.5643	-95		306	315	0	(K) (L)
1262.5191	1262.6163	-77		158	169	0	(R) (F)
1532.7231	1532.7603	-24		156	169	1	(K) (F)
1556.7461	1556.8145	-44		100	112	1	(R) (N)
1574.8318	1574.9158	-53	AcetN	1	14	1	(-)ATLKDQLIYNLLK(E)
1635.8160	1635.8382	-14		319	332	1	(K) (-)
1657.7609	1657.8536	-56		43	57	0	(K) (L)
1692.7933	1692.9147	-72		119	132	1	(K) (L)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 30% (102/332AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

2. 5/61 matches (8%).
Acc. #: Species: MOUSE Name: Meprin A alpha-subunit precursor (Endopeptidase-2) (MEP-1)
Index: MW: 84198 Da pI: 5.8

m/z
Submitted MH⁺
Matched Delta
ppm Modifications Start End Missed
Cleavages Database
Sequence
900.2673 900.3344 -75 557 562 0 (K) (S)
1532.7231 1532.8412 -77 92 104 1 (K) (S)
1638.8155 1638.7739 25 309 323 0 (K) (G)
1764.8496 1764.8153 19 25 40 0 (K) (D)
2235.2302 2235.1311 44 150 168 0 (K) (T)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 9% (69/747AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

3. 4/61 matches (6%).
Acc. #: Species: HUMAN Name: Alpha enolase, lung specific (2-phospho-D-glycerate hydro-lyase) (Non-neural enolase) (NNE) (Phosphopyruvate hydratase) (HLE1)
Index: MW: 49478 Da pI: 5.8

m/z
Submitted MH⁺
Matched Delta
ppm Modifications Start End Missed
Cleavages Database
Sequence
1556.7461 1556.7783 -21 249 262 0 (K) (D)
1638.8155 1638.8175 -1.2 380 394 0 (K) (L)
2235.2302 2235.1443 38 112 134 0 (K) (G)
2390.3626 2390.2203 60 36 58 1 (R) (T)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 16% (75/458AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

4. 7/61 matches (11%).
Acc. #: Species: HUMAN Name: Sp110 nuclear body protein (Speckled 110 kDa) (Transcriptional coactivator Sp110) (Interferon-induced protein 41/75)
Index: MW: 78505 Da pI: 9.2

m/z
Submitted MH⁺
Matched Delta
ppm Modifications Start End Missed
Cleavages Database
Sequence
1319.5787 1319.5434 27 1Met-ox 47 56 0 (R)MYMESLEACR(N)
1475.6885 1475.6445 30 1Met-ox 46 56 1 (K)RMYMESLEACR(N)
1628.7514 1628.8511 -61 626 638 0 (R) (D)
1635.8160 1635.8567 -25 373 388 1 (R) (L)
1638.8155 1638.7169 60 348 362 0 (R) (R)
1940.9638 1940.9104 28 608 623 0 (R) (E)
2211.1440 2211.0821 28 653 672 1 (K) (D)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 13% (91/689AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

5. 4/61 matches (6%).
Acc. #: Species: MOUSE Name: Putative pre-mRNA splicing factor RNA helicase (DEAH box protein 15)
Index: MW: 86612 Da pI: 6.8

m/z
Submitted MH⁺
Matched Delta
ppm Modifications Start End Missed
Cleavages Database
Sequence
957.3238 957.3988 -78 223 230 0 (R) (T)
2211.1440 2211.1351 4.0 617 635 0 (R) (Q)
2268.1593 2268.0827 34 235 253 1 (K) (Y)
2529.4215 2529.2158 81 517 538 0 (K) (A)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 8% (68/758AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

MS-Fit in ProteinProspector 4.0.4
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